##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050275_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8230698
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.377313807407344	31.0	31.0	34.0	28.0	34.0
2	31.46440314053559	33.0	31.0	34.0	28.0	34.0
3	31.55457325733492	33.0	31.0	34.0	28.0	34.0
4	35.081199553184916	37.0	35.0	37.0	32.0	37.0
5	35.008484335107426	37.0	35.0	37.0	32.0	37.0
6	35.02576998937393	37.0	35.0	37.0	32.0	37.0
7	34.953944610772986	37.0	35.0	37.0	32.0	37.0
8	34.935053746352985	37.0	35.0	37.0	32.0	37.0
9	36.59034507158445	39.0	37.0	39.0	32.0	39.0
10	36.487987167066514	39.0	37.0	39.0	32.0	39.0
11	36.51126891060758	39.0	37.0	39.0	32.0	39.0
12	36.40377535416802	39.0	37.0	39.0	32.0	39.0
13	36.43025597586013	39.0	37.0	39.0	32.0	39.0
14	37.660359546662995	40.0	38.0	41.0	32.0	41.0
15	37.63374977432048	40.0	38.0	41.0	32.0	41.0
16	37.61495161163731	40.0	38.0	41.0	32.0	41.0
17	37.59095911428168	40.0	38.0	41.0	32.0	41.0
18	37.54465356401122	40.0	38.0	41.0	32.0	41.0
19	37.5590977824724	40.0	38.0	41.0	32.0	41.0
20	37.51826455058854	40.0	38.0	41.0	32.0	41.0
21	37.47754686176069	40.0	38.0	41.0	32.0	41.0
22	37.43433825417966	40.0	38.0	41.0	32.0	41.0
23	37.36504328065493	40.0	38.0	41.0	32.0	41.0
24	37.30557870061567	40.0	38.0	41.0	32.0	41.0
25	37.01900993087099	40.0	37.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5139751840409446
1101	2	0.45238437246182883
1101	3	0.4612871653412789
1101	4	0.3619327660465572
1101	5	0.46421042238961974
1101	6	0.47890604535952264
1101	7	0.5073501285968973
1101	8	0.5342887065066719
1101	9	0.5405494194229306
1101	10	0.6005713172286207
1101	11	0.5661765637822
1101	12	0.6069122038416097
1101	13	0.6072645871525495
1101	14	0.6186477237280528
1101	15	0.69681626968503
1101	16	0.6794342631424897
1101	17	0.7118074911988685
1101	18	0.7776802168337866
1101	19	0.7271138562678345
1101	20	0.7397507023872478
1101	21	0.7191807932272098
1101	22	0.7341379184320616
1101	23	0.7543462495010544
1101	24	0.8051760009158784
1101	25	0.9003607919402583
1102	1	0.41560479213884705
1102	2	0.42747586884724953
1102	3	0.4086055134418949
1102	4	0.3739404783015914
1102	5	0.35151222424173767
1102	6	0.3987027261659435
1102	7	0.3938986367701034
1102	8	0.4687175063304352
1102	9	0.4637171904518027
1102	10	0.5385687571608457
1102	11	0.4905817734769329
1102	12	0.5648507892697836
1102	13	0.5251941666930193
1102	14	0.5603124930501551
1102	15	0.5968840172890282
1102	16	0.5097781440697489
1102	17	0.5513507978937113
1102	18	0.6062471338952093
1102	19	0.644750444642554
1102	20	0.6564078076746469
1102	21	0.5562723654945145
1102	22	0.5795738211357815
1102	23	0.6169566141884673
1102	24	0.6157110834920374
1102	25	0.7771230282600783
1103	1	0.422392020030653
1103	2	0.367952346030993
1103	3	0.4359448198026179
1103	4	0.35594430675649136
1103	5	0.3312294830434155
1103	6	0.3382544106567238
1103	7	0.41789501698235654
1103	8	0.4134692126949133
1103	9	0.42202393340785704
1103	10	0.4797854503095067
1103	11	0.5075038266442604
1103	12	0.48839389882716944
1103	13	0.46486420546007423
1103	14	0.5698095898215101
1103	15	0.6117805934833811
1103	16	0.6071995872492764
1103	17	0.5705706792303502
1103	18	0.644539710701018
1103	19	0.6553924485856939
1103	20	0.6334558471334404
1103	21	0.6046919705289895
1103	22	0.6823117453982732
1103	23	0.6415370876139619
1103	24	0.6771198413308639
1103	25	0.7779598436915975
1104	1	0.3887529786596531
1104	2	0.3870272001573909
1104	3	0.3724501815657142
1104	4	0.2888779252571183
1104	5	0.36276839087098267
1104	6	0.3597111898454628
1104	7	0.40995021264580345
1104	8	0.3771401351287196
1104	9	0.43146666707739456
1104	10	0.45616617153558536
1104	11	0.439354907979876
1104	12	0.4584639204435845
1104	13	0.5260799084283576
1104	14	0.645421835536844
1104	15	0.5921241331052656
1104	16	0.603565477945061
1104	17	0.626236090565861
1104	18	0.6312500221535942
1104	19	0.6740745110821891
1104	20	0.6582632276109237
1104	21	0.6722149401138111
1104	22	0.6064411669503755
1104	23	0.6063832380271208
1104	24	0.6732039405077472
1104	25	0.8076749739221967
1105	1	0.3156685746996146
1105	2	0.2582266287103394
1105	3	0.25925442159323353
1105	4	0.22176097953519047
1105	5	0.22694136609707272
1105	6	0.33504980935085626
1105	7	0.3325742196362427
1105	8	0.38199119703968165
1105	9	0.40824657152922583
1105	10	0.4411173673376254
1105	11	0.4771365146313826
1105	12	0.5033662682299251
1105	13	0.478779614945104
1105	14	0.538525473094353
1105	15	0.5446304440547465
1105	16	0.5328414559291161
1105	17	0.5495589904971396
1105	18	0.5461528654787315
1105	19	0.5502486472393571
1105	20	0.6625701927034839
1105	21	0.663860649950827
1105	22	0.6121603777785509
1105	23	0.6190679955246168
1105	24	0.6404602983422052
1105	25	0.6572366849937552
1106	1	0.2285764661137648
1106	2	0.22548586393124737
1106	3	0.19884712311830555
1106	4	0.19056933536575116
1106	5	0.16388484441037576
1106	6	0.22073228879167317
1106	7	0.2662161371789793
1106	8	0.262853810189533
1106	9	0.29927625858522333
1106	10	0.30644400702274766
1106	11	0.30845950599788097
1106	12	0.3608866144114131
1106	13	0.3483722715722948
1106	14	0.36768334225534005
1106	15	0.3195964091084278
1106	16	0.3357405358722545
1106	17	0.35899971842341927
1106	18	0.3772671730408348
1106	19	0.4314252062414212
1106	20	0.4194499452555718
1106	21	0.3837923404287764
1106	22	0.4441067971643733
1106	23	0.45814115803820243
1106	24	0.5311717194297074
1106	25	0.6123437724538476
1107	1	0.2013574366365205
1107	2	0.17417627191784035
1107	3	0.1647409300594056
1107	4	0.18868946422075084
1107	5	0.2453653471792805
1107	6	0.26039369459376616
1107	7	0.2534474795011121
1107	8	0.2713212857155227
1107	9	0.31567878043348685
1107	10	0.3132493646722949
1107	11	0.30533520874530495
1107	12	0.345934111423567
1107	13	0.2718396270422403
1107	14	0.3418173573348966
1107	15	0.24332302109321802
1107	16	0.23186350011543766
1107	17	0.29381821820822296
1107	18	0.3158363915482596
1107	19	0.24753064746546727
1107	20	0.2575824695746647
1107	21	0.1362944504460728
1107	22	0.05564889513991744
1107	23	0.2169848608145415
1107	24	0.2472882497015192
1107	25	0.4057752866755848
1108	1	0.1705858859131979
1108	2	0.15563581036539986
1108	3	0.1454198573436969
1108	4	0.14185430870422522
1108	5	0.18831233729608243
1108	6	0.15548010948619861
1108	7	0.13751549084811643
1108	8	0.13695382556631586
1108	9	0.20101601051314333
1108	10	0.15901808874025392
1108	11	0.21237332821187493
1108	12	0.15700374809740225
1108	13	0.13315495559044876
1108	14	0.14203932549464326
1108	15	0.16369778007037894
1108	16	0.15151203580700923
1108	17	0.15846698104174095
1108	18	0.11942889384491906
1108	19	0.08004248408478531
1108	20	0.10470495965613935
1108	21	0.21287561078809603
1108	22	0.20613195035055298
1108	23	0.10932967238768043
1108	24	0.1476757791037855
1108	25	0.34203900352547834
1109	1	-0.032154451986830423
1109	2	0.01360153649372009
1109	3	-0.028540736425338054
1109	4	-0.06407007768910944
1109	5	0.0017628043131878712
1109	6	0.032268847071662776
1109	7	0.02292320200114517
1109	8	0.0085646009585858
1109	9	0.06190157900471149
1109	10	-0.012119411777121059
1109	11	0.005942554948234147
1109	12	-0.07171740948218996
1109	13	0.04569127038303833
1109	14	-0.008168683863438275
1109	15	0.038574915843355484
1109	16	-0.026231825169915624
1109	17	-0.012150188119164795
1109	18	-0.04399903040744846
1109	19	-0.004029217181944489
1109	20	0.014510914493740756
1109	21	0.023976801440269924
1109	22	0.028306344134961137
1109	23	0.015976708371354675
1109	24	0.029634792169225932
1109	25	0.21765709410649237
1110	1	-0.02794348117739176
1110	2	-0.10999380151627847
1110	3	-0.025417542775073798
1110	4	-0.025523996416396244
1110	5	0.0389637089297139
1110	6	0.1027492385669504
1110	7	0.08545294466805586
1110	8	0.07938815923087361
1110	9	0.05782469128714496
1110	10	0.007685383553777569
1110	11	0.07177735305552346
1110	12	0.09935686854676362
1110	13	0.11010042244761564
1110	14	0.1026515530935157
1110	15	0.06646429660896302
1110	16	0.14321182472614424
1110	17	0.13836748199131677
1110	18	0.1542469713431487
1110	19	0.0790534712650981
1110	20	0.10747510200342703
1110	21	0.11214309943540002
1110	22	0.10720411178331801
1110	23	0.14112830869162707
1110	24	0.15784193423743886
1110	25	0.28970246380131215
1111	1	0.024245108245285252
1111	2	-0.008305849340224825
1111	3	-0.05749605350090903
1111	4	0.006880316704240386
1111	5	0.003330841364373782
1111	6	0.011026419843986446
1111	7	0.03814305841125076
1111	8	0.03277614428604636
1111	9	0.0672288461451842
1111	10	0.05174162145618766
1111	11	0.0640641618818023
1111	12	0.04830552100381169
1111	13	0.11300685669104382
1111	14	0.06293972370971801
1111	15	0.06628533961323058
1111	16	0.05000016246372496
1111	17	0.09385108945159715
1111	18	0.07520526244383774
1111	19	0.03884366705187148
1111	20	0.09090412534439452
1111	21	0.09108742627148558
1111	22	0.054928763664797486
1111	23	0.10439218182791166
1111	24	0.011400512448794586
1111	25	0.20015590513035164
1112	1	-0.14409914024371417
1112	2	-0.14405601401137602
1112	3	-0.1770586308735176
1112	4	-0.09638037883068051
1112	5	-0.07418072842418155
1112	6	-0.013446845932946871
1112	7	-0.10091180495942353
1112	8	-0.07632968836860243
1112	9	-0.039375011253575565
1112	10	-0.07571280630458688
1112	11	-0.11632611599545584
1112	12	-0.06851996308137842
1112	13	-0.1125519136669908
1112	14	-0.13221951815871336
1112	15	-0.10806164362772108
1112	16	-0.12966866316838832
1112	17	-0.08074028398284838
1112	18	-0.06240531027163598
1112	19	-0.06889999648252143
1112	20	-0.0967792029740906
1112	21	-0.06896292576140439
1112	22	-0.04533405902755305
1112	23	-0.04069548675463608
1112	24	0.050034209994436196
1112	25	0.12650900972217016
1113	1	-0.24822795569969358
1113	2	-0.25624899944341095
1113	3	-0.24979548061865842
1113	4	-0.24409145528411358
1113	5	-0.2229572585488242
1113	6	-0.17012333698621518
1113	7	-0.24846540920570703
1113	8	-0.22299564791570248
1113	9	-0.2344747438044621
1113	10	-0.24440083110387434
1113	11	-0.27739399401104237
1113	12	-0.2681715043212378
1113	13	-0.29034810062915994
1113	14	-0.30931997429444635
1113	15	-0.3345955533009928
1113	16	-0.30463316220065195
1113	17	-0.2870082401870988
1113	18	-0.2668195680607397
1113	19	-0.21092146127221412
1113	20	-0.23389547909300035
1113	21	-0.31336527713213513
1113	22	-0.2584217978450951
1113	23	-0.1991741112768537
1113	24	-0.2618875780212804
1113	25	-0.1630092265487164
1114	1	-0.3240043975614171
1114	2	-0.2820231313901367
1114	3	-0.37890050998836244
1114	4	-0.27224792673465714
1114	5	-0.31828630913541645
1114	6	-0.30496845946831996
1114	7	-0.2987670950601853
1114	8	-0.3713584294696872
1114	9	-0.3158076040160722
1114	10	-0.4476990519032569
1114	11	-0.43040963368767393
1114	12	-0.3709070508270358
1114	13	-0.33926637108897495
1114	14	-0.377968251298725
1114	15	-0.5262040064577462
1114	16	-0.49927840034284543
1114	17	-0.4591425121676238
1114	18	-0.44087668449798656
1114	19	-0.47786016836257517
1114	20	-0.41800348761393735
1114	21	-0.37047680694957563
1114	22	-0.40906977465171934
1114	23	-0.4668846084436069
1114	24	-0.5388506314330854
1114	25	-0.47010836225704367
1115	1	-0.35984569564720204
1115	2	-0.33870484278465796
1115	3	-0.34634823426439354
1115	4	-0.2735719920723625
1115	5	-0.3708720128972871
1115	6	-0.33245877265476764
1115	7	-0.377346686648508
1115	8	-0.3570369584812241
1115	9	-0.4843977592794957
1115	10	-0.5000394631011957
1115	11	-0.4627136925100501
1115	12	-0.442117113609271
1115	13	-0.5028695851691367
1115	14	-0.6373712281953132
1115	15	-0.616783540702663
1115	16	-0.6758707747607886
1115	17	-0.6329999272677256
1115	18	-0.6324544864796025
1115	19	-0.5944475744847395
1115	20	-0.5962451005255645
1115	21	-0.6298567849198591
1115	22	-0.5801787740832154
1115	23	-0.596599278398898
1115	24	-0.6429977292236444
1115	25	-0.5629308415043539
1116	1	-0.17423830515448202
1116	2	-0.1537214100859181
1116	3	-0.1580916806747048
1116	4	-0.14655198535887592
1116	5	-0.21745191697557686
1116	6	-0.1985548593598736
1116	7	-0.2225911567256773
1116	8	-0.2959868832107304
1116	9	-0.2804139795563714
1116	10	-0.2917825910317262
1116	11	-0.3194114070025762
1116	12	-0.23257029254993
1116	13	-0.29652926682318537
1116	14	-0.30871970292703566
1116	15	-0.37085027763252043
1116	16	-0.32490264704836846
1116	17	-0.34152115449568043
1116	18	-0.290517526333538
1116	19	-0.3482044924203791
1116	20	-0.3741295934993971
1116	21	-0.3485061350389955
1116	22	-0.3846872873611531
1116	23	-0.39652776020663083
1116	24	-0.45364771426949346
1116	25	-0.31746276219916325
1201	1	0.3705284377932401
1201	2	0.39155621542925445
1201	3	0.37697901083681273
1201	4	0.2824298781018726
1201	5	0.34489204343495317
1201	6	0.3577266030261157
1201	7	0.4122653641564469
1201	8	0.3652134354671688
1201	9	0.42558013556218555
1201	10	0.41769108681327083
1201	11	0.44921737116154503
1201	12	0.5049519387496986
1201	13	0.503314241573662
1201	14	0.5622307411211622
1201	15	0.5749998487587078
1201	16	0.5321851220478919
1201	17	0.49329597111452017
1201	18	0.5359194432022463
1201	19	0.5418898234008225
1201	20	0.5458930773276762
1201	21	0.5349209147766203
1201	22	0.5708815615381866
1201	23	0.5726635935068245
1201	24	0.5610055102632643
1201	25	0.5370584322294434
1202	1	0.3029202352359519
1202	2	0.3138476098726173
1202	3	0.3303563166245098
1202	4	0.24159790734032782
1202	5	0.19504905389782579
1202	6	0.2868157638049311
1202	7	0.31910702106427635
1202	8	0.3566194147639905
1202	9	0.41353112424247485
1202	10	0.4332087849885795
1202	11	0.42728205994075097
1202	12	0.4452883286516709
1202	13	0.40889236239240034
1202	14	0.4580061956219268
1202	15	0.3826483407693715
1202	16	0.41558191286361534
1202	17	0.3906651557288683
1202	18	0.40081777134869867
1202	19	0.36022744565479314
1202	20	0.4600354557332693
1202	21	0.4468881095335462
1202	22	0.4229407031506369
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2210	12	-0.17458481328933573
2210	13	-0.165235754603259
2210	14	-0.12433145911887067
2210	15	-0.12230991288699755
2210	16	-0.1171139571848272
2210	17	-0.08494410965846555
2210	18	-0.058667352341238654
2210	19	-0.09058235335584186
2210	20	-0.024679572561574048
2210	21	-0.08407772663381508
2210	22	-0.05148720147101926
2210	23	-0.0732672849807372
2210	24	-0.10055995382184335
2210	25	-0.20715381420551893
2211	1	-0.12866046054974234
2211	2	-0.1087056608131256
2211	3	-0.09431116267760231
2211	4	-0.10265489401248118
2211	5	-0.08511797642705687
2211	6	-0.12709760757772415
2211	7	-0.13807061671818133
2211	8	-0.1645711460989645
2211	9	-0.20256758190086543
2211	10	-0.20478459456467135
2211	11	-0.19950205898168605
2211	12	-0.19293166016520757
2211	13	-0.2231265373422886
2211	14	-0.19184938972217225
2211	15	-0.2459880185975365
2211	16	-0.2185891269870126
2211	17	-0.2401617113930996
2211	18	-0.20289504549452175
2211	19	-0.22970630044859774
2211	20	-0.17155386550223994
2211	21	-0.18923100768813583
2211	22	-0.2678111004349617
2211	23	-0.23557792931040922
2211	24	-0.24754325468947513
2211	25	-0.251318753335525
2212	1	-0.27410620122004303
2212	2	-0.22460855841110217
2212	3	-0.2381088493005521
2212	4	-0.21232488279982675
2212	5	-0.21243971904162606
2212	6	-0.251703486231861
2212	7	-0.26570685968882657
2212	8	-0.25865816487801396
2212	9	-0.3394843602786537
2212	10	-0.3047468490742844
2212	11	-0.30453067350904917
2212	12	-0.269132403253586
2212	13	-0.216237695938851
2212	14	-0.29610007806835625
2212	15	-0.2762719480345126
2212	16	-0.29215435913437204
2212	17	-0.3143455596462843
2212	18	-0.31180345108769103
2212	19	-0.25476340216421534
2212	20	-0.23851047037743456
2212	21	-0.28214643858665056
2212	22	-0.3384307134629978
2212	23	-0.3221015839455035
2212	24	-0.36496843343067553
2212	25	-0.38388656072906
2213	1	-0.4413634661293848
2213	2	-0.40111482256732245
2213	3	-0.3771086334048839
2213	4	-0.3705949127499082
2213	5	-0.34019743788115875
2213	6	-0.40433666649563804
2213	7	-0.4379754093837107
2213	8	-0.3983049326358312
2213	9	-0.47175622778690496
2213	10	-0.49790466117163135
2213	11	-0.493724274717799
2213	12	-0.47370647753977835
2213	13	-0.46122505468812136
2213	14	-0.4856150777933834
2213	15	-0.5241602499256501
2213	16	-0.5109737293500274
2213	17	-0.5422172918965416
2213	18	-0.5080747359522775
2213	19	-0.5141418014910926
2213	20	-0.5024343845371035
2213	21	-0.5410769364589427
2213	22	-0.6124849775632129
2213	23	-0.5741774278326375
2213	24	-0.6164018487079019
2213	25	-0.5396815273657154
2214	1	-0.33492715086467584
2214	2	-0.4060391518015649
2214	3	-0.39185140898698734
2214	4	-0.32731489173449546
2214	5	-0.3251749660762613
2214	6	-0.3393249636113893
2214	7	-0.4067862987585613
2214	8	-0.4618943494236163
2214	9	-0.4637395672563045
2214	10	-0.46962793983236395
2214	11	-0.4964695362695011
2214	12	-0.5029789667178761
2214	13	-0.5074601956722731
2214	14	-0.5671483749510244
2214	15	-0.5998171171209563
2214	16	-0.583849356448674
2214	17	-0.5962408021506675
2214	18	-0.5985301701172077
2214	19	-0.5598554014303687
2214	20	-0.5630153049668536
2214	21	-0.5975010535183429
2214	22	-0.681637219544065
2214	23	-0.6736905839050991
2214	24	-0.7081597062003411
2214	25	-1.489854939023843
2215	1	-0.5619585541636702
2215	2	-0.5616439546590577
2215	3	-0.5549333702943215
2215	4	-0.43130967190374037
2215	5	-0.5455407374768058
2215	6	-0.6003432330190392
2215	7	-0.5917830219925548
2215	8	-0.6072116400171907
2215	9	-0.7088983850144643
2215	10	-0.6679241173219381
2215	11	-0.6963397111836542
2215	12	-0.7254637134679385
2215	13	-0.7733098745108791
2215	14	-0.8367419026674838
2215	15	-0.8301524011839945
2215	16	-0.8116840464781063
2215	17	-0.8609201389792176
2215	18	-0.8137725933144111
2215	19	-0.8092749731129771
2215	20	-0.8543869668812505
2215	21	-0.9257555911685742
2215	22	-0.8512874678884401
2215	23	-0.8950026891601865
2215	24	-0.851366357077211
2215	25	-0.9033875993745895
2216	1	-0.41390254844027297
2216	2	-0.3234559954711571
2216	3	-0.31142616443515436
2216	4	-0.24625868099997206
2216	5	-0.26275064564590167
2216	6	-0.33941095087354967
2216	7	-0.34829923279537667
2216	8	-0.3513741365762044
2216	9	-0.40204427480669125
2216	10	-0.39296390789952085
2216	11	-0.4471344056675193
2216	12	-0.3674481952888442
2216	13	-0.4396973055742279
2216	14	-0.514526336915921
2216	15	-0.48357136537702416
2216	16	-0.4287343037502822
2216	17	-0.5197761221858741
2216	18	-0.4671459769696611
2216	19	-0.43319204384495436
2216	20	-0.43868558241887
2216	21	-0.4358812773613323
2216	22	-0.5622324846056159
2216	23	-0.5516277837873105
2216	24	-0.5809094964358081
2216	25	-0.7090948464587754
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80550.0
3	7.0
4	1470.0
5	12235.0
6	14639.0
7	11375.0
8	9509.0
9	8040.0
10	7429.0
11	6908.0
12	6377.0
13	6224.0
14	6306.0
15	6494.0
16	6889.0
17	7380.0
18	8348.0
19	9721.0
20	12461.0
21	16074.0
22	20600.0
23	25498.0
24	30917.0
25	37139.0
26	43596.0
27	50552.0
28	60068.0
29	75108.0
30	99119.0
31	132849.0
32	177368.0
33	241749.0
34	339433.0
35	486426.0
36	731176.0
37	1308661.0
38	4132003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.669305480911504	18.506435026612987	13.250136303895339	25.574123188580177
2	16.999276589819623	21.225248351793617	36.57162866434472	25.203846394042035
3	18.434631769944872	24.669866291580128	28.683318179283084	28.212183759191916
4	13.04233006097952	16.943816986715746	36.9347966860496	33.07905626625513
5	15.53458112901946	35.5714980317183	32.80423724086438	16.089683598397862
6	32.561807590811256	37.24051756636791	16.127324245114373	14.07035059770646
7	29.256553677893255	30.873437953588518	21.25102783006413	18.618980538454093
8	27.144650495819562	32.099358351156916	19.780490472486875	20.97550068053665
9	27.37361923832301	15.017973391067297	18.920825917856	38.6875814527537
10	16.698074042818387	25.9133579342251	30.07332627811776	27.315241744838747
11	36.620360128648635	22.244519913553564	22.19428325550075	18.940836702297055
12	25.5019550724687	24.008450346798693	27.641025067612013	22.848569513120594
13	29.349512630821522	20.166921300892117	24.75932873768576	25.7242373306006
14	24.296636535217928	20.41239398122712	24.759045205921186	30.53192427763377
15	25.00370879546464	26.835410279043575	22.787094485285408	25.37378644020638
16	25.53021128746514	26.348839800392476	23.192485958375286	24.9284629537671
17	24.95944361204085	25.46823309741455	24.28751733184165	25.284805958702954
18	26.68858451971337	23.77149477870473	25.359637996998696	24.1802827045832
19	27.307720438776883	24.497740045862454	24.561343400037273	23.63319611532339
20	26.27018201615861	24.299070303223374	24.383268981486374	25.047478699131638
21	26.87251661100506	24.454055359649157	24.28206487720103	24.391363152144756
22	25.665065101404522	24.258647322499257	25.10217237954788	24.974115196548336
23	24.372334140029277	23.54398347745445	25.54569827838312	26.53798410413315
24	24.935044422507406	25.269721315209964	24.894026881312172	24.90120738097046
25	24.95689331030416	24.413377882794094	25.102196621993087	25.527532184908658
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1905.0
1	1905.0
2	2027.5
3	2150.0
4	2150.0
5	2150.0
6	5120.0
7	8090.0
8	8090.0
9	8090.0
10	11201.0
11	14312.0
12	14312.0
13	14312.0
14	20798.5
15	27285.0
16	27285.0
17	27285.0
18	51235.5
19	75186.0
20	75186.0
21	75186.0
22	114029.5
23	152873.0
24	152873.0
25	152873.0
26	213651.0
27	274429.0
28	274429.0
29	274429.0
30	326700.0
31	378971.0
32	378971.0
33	378971.0
34	466925.5
35	554880.0
36	554880.0
37	554880.0
38	615381.0
39	675882.0
40	675882.0
41	675882.0
42	765726.5
43	855571.0
44	855571.0
45	855571.0
46	918600.5
47	981630.0
48	981630.0
49	981630.0
50	1001662.0
51	1021694.0
52	1021694.0
53	1021694.0
54	950286.0
55	878878.0
56	878878.0
57	878878.0
58	825493.5
59	772109.0
60	772109.0
61	772109.0
62	684186.0
63	596263.0
64	596263.0
65	596263.0
66	499435.0
67	402607.0
68	402607.0
69	402607.0
70	313438.5
71	224270.0
72	224270.0
73	224270.0
74	184258.5
75	144247.0
76	144247.0
77	144247.0
78	123576.0
79	102905.0
80	102905.0
81	102905.0
82	76598.5
83	50292.0
84	50292.0
85	50292.0
86	37178.5
87	24065.0
88	24065.0
89	24065.0
90	16712.5
91	9360.0
92	9360.0
93	9360.0
94	5025.0
95	690.0
96	690.0
97	690.0
98	422.0
99	154.0
100	154.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03353300048185464
2	0.0028673145339556867
3	0.009415969338201937
4	0.16617059695301664
5	0.023655344905134414
6	0.09231294842794621
7	0.05625282327209673
8	0.023060012650202934
9	0.04296111945791232
10	0.02757967793253986
11	0.04420038251919825
12	0.034869460645986526
13	0.007192585610600705
14	0.0038392855624152407
15	0.003195354756060786
16	0.0042159243359433185
17	0.002211234089745487
18	0.0032682525831952526
19	0.0
20	0.0020046902461978316
21	1.2149637855744433E-4
22	0.0
23	6.074818927872217E-5
24	8.869235634693436E-4
25	0.15449479497364624
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8230698.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.26989245022582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.85406918913675	32.55984670409654
2	11.849689312813231	9.543714283911584
3	2.969775757881897	3.5877765111357562
4	1.2216048130820512	1.967755777579697
5	0.6421588884382234	1.292983468668191
6	0.4158803136306402	1.004847330124324
7	0.28112023000417624	0.7924476999495645
8	0.20890145723588832	0.6729951372467737
9	0.17988507927878483	0.651955751636131
>10	1.1075330764806681	8.72958708784375
>50	0.12809380553572722	3.6153842785768053
>100	0.10432703642643133	8.603588750818354
>500	0.01704037009612302	4.787797177202066
>1k	0.017495017605593716	14.67859760936343
>5k	0.0020011631920173493	5.3607444969192635
>10k+	4.244891619430741E-4	2.1499779349277235
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	19232	0.23366183524167694	No Hit
GTACAAAGGGCAGGGACTTAATCAA	18105	0.21996919337825296	No Hit
GAATAGGACCGCGGTTCTATTTTGT	14024	0.17038652128895992	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	12896	0.15668172978768022	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	12260	0.14895456011142677	No Hit
CTATTGGAGCTGGAATTACCGCGGC	12246	0.14878446518144633	No Hit
GTATCTGATCGTCTTCGAACCTCCG	12206	0.14829847966721657	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	11307	0.1373759552349023	No Hit
TATCAACGCAGAGTACTTTTTTTTT	11305	0.13735165595919083	No Hit
ATCAGATACCGTCGTAGTTCCGACC	10796	0.1311674902906169	No Hit
GTCCTATTCCATTATTCCTAGCTGC	10547	0.12814223046453654	No Hit
GAACTACGACGGTATCTGATCGTCT	10461	0.12709736160894253	No Hit
TCGTAGTTCCGACCATAAACGATGC	10353	0.12578520072052213	No Hit
CTTTAATATACGCTATTGGAGCTGG	10344	0.12567585397982042	No Hit
GTATCAACGCAGAGTACGGGCGGTG	9914	0.12045150970185033	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	9902	0.12030571404758139	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	9722	0.1181187792335474	No Hit
CTTATGACCCGCACTTACTGGGAAT	9367	0.11380565779475812	No Hit
GACTTAATCAACGCAAGCTTATGAC	8979	0.10909159830672928	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	8959	0.10884860554961437	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	8957	0.10882430627390288	No Hit
GAATAACGCCGCCGCATCGCCAGTC	8889	0.10799813089971226	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	8808	0.10701401023339696	No Hit
GCTTTGAACACTCTAATTTTTTCAA	8339	0.10131583007905283	No Hit
GAATAATGGAATAGGACCGCGGTTC	8302	0.10086629347839028	No Hit
GAATAATTGCAATCCCCGATCCCCA	8295	0.10078124601340009	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2149637855744433E-5
2	0.0	0.0	0.0	0.0	1.2149637855744433E-5
3	0.0	0.0	0.0	0.0	1.2149637855744433E-5
4	0.0	0.0	0.0	0.0	1.2149637855744433E-5
5	0.0	0.0	0.0	1.2149637855744433E-5	1.2149637855744433E-5
6	1.2149637855744433E-5	0.0	0.0	1.2149637855744433E-5	2.4299275711488866E-5
7	2.4299275711488866E-5	0.0	0.0	1.2149637855744433E-5	2.4299275711488866E-5
8	2.4299275711488866E-5	0.0	0.0	1.2149637855744433E-5	2.4299275711488866E-5
9	2.4299275711488866E-5	0.0	0.0	6.074818927872217E-5	2.4299275711488866E-5
10	2.4299275711488866E-5	0.0	0.0	8.504746499021104E-5	4.859855142297773E-5
11	2.4299275711488866E-5	0.0	0.0	8.504746499021104E-5	4.859855142297773E-5
12	2.4299275711488866E-5	0.0	0.0	8.504746499021104E-5	1.093467407016999E-4
13	2.4299275711488866E-5	0.0	0.0	8.504746499021104E-5	1.4579565426893322E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5075	0.0	16.487968	1
GTATCAA	17285	0.0	13.54815	1
CGTCGTA	2040	0.0	12.1481285	10
CTCGACG	295	0.0	11.904826	13
CCGTCGT	2095	0.0	11.784097	9
CGCCAGT	1660	0.0	11.664127	18
ATACCGT	2215	0.0	11.32252	6
ATCGCCA	1730	0.0	11.302032	16
CGCTTAC	320	0.0	11.271034	18
GTCGTAG	2165	0.0	11.227449	11
CGAACGA	970	0.0	11.154974	16
ACCGTCG	2265	0.0	11.15117	8
TACCGTC	2235	0.0	11.13213	7
TCGCCAG	1775	0.0	11.1223135	17
CGGTTCT	2480	0.0	11.025684	12
AACCGCG	1140	0.0	10.995717	7
TCGACGC	320	0.0	10.974562	14
TAATACC	560	0.0	10.876981	4
GCATCGC	1800	0.0	10.809779	14
CGGTCCA	1965	0.0	10.775598	10
>>END_MODULE