FastQCFastQC Report
Thu 2 Feb 2017
SRR4050274_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050274_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903207
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA358603.9702969529686993No Hit
CTTCCGTACTCTGCGTTGATACCAC215162.3821781717812196No Hit
GAGTACGGAAGCAGTGGTATCAACG161761.790951575884598No Hit
TCCGTACTCTGCGTTGATACCACTG148351.6424806273644912No Hit
GCTTCCGTACTCTGCGTTGATACCA114821.2712478977687285No Hit
GTACATGGAAGCAGTGGTATCAACG88200.9765203325483527No Hit
GCGTTGATACCACTGCTTCCGTACT79420.8793111656574849No Hit
GTATCAACGCAGAGTACGGAAGCAG71640.7931736578658048No Hit
TCCATGTACTCTGCGTTGATACCAC66360.7347152978220939No Hit
TATCAACGCAGAGTACGGAAGCAGT53210.589122980667776No Hit
GTATCAACGCAGAGTACTTTTTTTT47760.5287824385772032No Hit
ACTCTGCGTTGATACCACTGCTTCC38900.43068753895840045No Hit
GTACAAGCAGTGGTATCAACGCAGA38730.4288053569115386No Hit
GGTATCAACGCAGAGTACGGAAGCA37920.41983731304119654No Hit
CAGTGGTATCAACGCAGAGTACGGA37920.41983731304119654No Hit
CCCATGTACTCTGCGTTGATACCAC36300.40190122530051253No Hit
ATACCACTGCTTCCGTACTCTGCGT34700.38418657074181223No Hit
ACGCAGAGTACGGAAGCAGTGGTAT30940.3425571325288666No Hit
GTACATGGGAAGCAGTGGTATCAAC30060.3328140725215814No Hit
TATCAACGCAGAGTACTTTTTTTTT29180.32307101251429626No Hit
GAGTACATGGAAGCAGTGGTATCAA27470.30413847545468536No Hit
AAGCAGTGGTATCAACGCAGAGTAC26900.29782762976814836No Hit
GAAGCAGTGGTATCAACGCAGAGTA25170.2786736595265537No Hit
GTGGTATCAACGCAGAGTACGGAAG23720.26261975383273156No Hit
CATGTACTCTGCGTTGATACCACTG23090.25564460860024335No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22690.2512159449605683No Hit
GCTTGTACTCTGCGTTGATACCACT21820.24158360154427502No Hit
GTACTCTGCGTTGATACCACTGCTT21170.23438702312980303No Hit
GGTATCAACGCAGAGTACTTTTTTT18840.20859005742869574No Hit
GAGTACAAGCAGTGGTATCAACGCA18610.2060435758358826No Hit
GTTGATACCACTGCTTCCGTACTCT18230.20183634537819126No Hit
GATACCACTGCTTCCGTACTCTGCG17390.19253615173487362No Hit
CCACTGCTTCCGTACTCTGCGTTGA17000.18821820468619044No Hit
ATCAACGCAGAGTACGGAAGCAGTG16810.18611458945734477No Hit
GCTTCCATGTACTCTGCGTTGATAC16630.184121690819491No Hit
CATGGAAGCAGTGGTATCAACGCAG16450.18212879218163722No Hit
AAAAAGTACTCTGCGTTGATACCAC16200.17936087740684029No Hit
CGGAAGCAGTGGTATCAACGCAGAG15550.1721642989923683No Hit
CTGCTTCCGTACTCTGCGTTGATAC14520.160760490120205No Hit
GCGTTGATACCACTGCTTCCATGTA13880.1536746282967249No Hit
GTATCAACGCAGAGTACATGGAAGC13100.1450387341993585No Hit
GTATCAACGCAGAGTACATGGGAAG11980.1326384760082683No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11820.13086701055239827No Hit
GCAGAGTACGGAAGCAGTGGTATCA11810.1307562939614064No Hit
GTACGGGAAGCAGTGGTATCAACGC11510.1274347962316501No Hit
CACTGCTTCCGTACTCTGCGTTGAT10780.11935248508924311No Hit
CAGTGGTATCAACGCAGAGTACATG10630.11769173622436496No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10580.11713815326940558No Hit
TATCAACGCAGAGTACATGGAAGCA10520.11647385372345431No Hit
TGATACCACTGCTTCCGTACTCTGC10390.11503453804055992No Hit
CTGCTTGTACTCTGCGTTGATACCA9540.1056236278062504No Hit
GCAGTGGTATCAACGCAGAGTACGG9520.10540219462426666No Hit
AAAGTACTCTGCGTTGATACCACTG9450.1046271784873235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG402.728995E-416.6492337
CCGCCTG702.4812834E-816.29492419
CGAACGT350.00223195716.2163754
CGTCGTA601.4430461E-615.85641410
GCCGCCT601.4565194E-615.84228818
CGAGCCG551.1206348E-515.55511315
CCCGTCC456.684163E-414.7993188
CCGTCGT653.3177585E-614.6366899
TCGAACG400.00542831214.1893283
CCGACCA759.502237E-713.9536449
TGGTTGA903.6025085E-813.73074714
ACCGTCG707.14604E-613.591218
TACCGTC707.14604E-613.591217
AGAACCG500.001481703613.3201285
CGTTCAG500.001482326113.31938710
GTCGCTC500.001491690413.30826316
CGACCAT801.9643285E-613.0815410
GCCAGTA801.9643285E-613.0815411
CGTCTTC1051.9490471E-812.6851310
CCGCGGC1001.4292164E-712.35698419
Produced by FastQC (version 0.11.5)