##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050274_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	903207
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.230786519590747	31.0	31.0	34.0	26.0	34.0
2	30.35030618673239	31.0	31.0	34.0	26.0	34.0
3	30.473131851281046	31.0	31.0	34.0	26.0	34.0
4	33.93799206604909	37.0	35.0	37.0	29.0	37.0
5	33.95006681746266	37.0	35.0	37.0	30.0	37.0
6	33.892838518744874	37.0	35.0	37.0	30.0	37.0
7	33.74398449081993	37.0	35.0	37.0	29.0	37.0
8	33.63305310964153	37.0	35.0	37.0	28.0	37.0
9	35.005956552595364	39.0	35.0	39.0	27.0	39.0
10	34.95684156566546	39.0	35.0	39.0	27.0	39.0
11	34.77365321570803	39.0	35.0	39.0	27.0	39.0
12	34.72028781884994	39.0	35.0	39.0	27.0	39.0
13	34.691349823462396	39.0	35.0	39.0	27.0	39.0
14	35.81550187277114	40.0	36.0	41.0	27.0	41.0
15	35.80741291863327	40.0	36.0	41.0	27.0	41.0
16	35.76482578190824	40.0	36.0	41.0	27.0	41.0
17	35.823804509929616	40.0	36.0	41.0	27.0	41.0
18	35.78395760883164	40.0	36.0	41.0	27.0	41.0
19	35.855698638296644	40.0	37.0	41.0	27.0	41.0
20	35.83202964547441	40.0	37.0	41.0	27.0	41.0
21	35.77461202138602	40.0	37.0	41.0	27.0	41.0
22	35.73173923585623	40.0	36.0	41.0	27.0	41.0
23	35.655106747401206	40.0	36.0	41.0	27.0	41.0
24	35.593734326682586	40.0	36.0	41.0	27.0	41.0
25	35.380705641121025	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.612586219021992
1101	2	0.5743659993123273
1101	3	0.3979259292773598
1101	4	0.5641241085583317
1101	5	0.6080693672061486
1101	6	0.6094858592461847
1101	7	0.5384472605644817
1101	8	0.6550845429749117
1101	9	0.8118774736261898
1101	10	0.683275628659068
1101	11	0.5520997621411823
1101	12	0.7676535452686863
1101	13	0.917463481329122
1101	14	0.8713350021719393
1101	15	0.9836163370970965
1101	16	1.186372426213154
1101	17	1.0209730872872953
1101	18	0.9076033264204568
1101	19	0.8663588051104796
1101	20	0.9581529479152167
1101	21	0.9820952181890803
1101	22	0.9215793064529123
1101	23	0.9764681603627352
1101	24	0.8939964043655948
1101	25	1.0197294592581372
1102	1	0.68285670858819
1102	2	0.6087328776793086
1102	3	0.6042802580693376
1102	4	0.5674981591647921
1102	5	0.724293700694389
1102	6	0.6416865590128467
1102	7	0.6326176338307405
1102	8	0.45820755290588977
1102	9	0.4933146015831724
1102	10	0.5221006304416775
1102	11	0.45671475524432026
1102	12	0.7964304042851253
1102	13	0.5950052989029473
1102	14	0.7364307590681776
1102	15	0.6915067840281779
1102	16	0.7065220671094252
1102	17	0.8118993560753864
1102	18	0.6474301550710564
1102	19	0.682922695085324
1102	20	0.6174641965536765
1102	21	0.730651304019915
1102	22	0.7020139928487694
1102	23	0.6702557027458269
1102	24	0.686572509522378
1102	25	0.6826452684278834
1103	1	0.5868765433934158
1103	2	0.6911230574500955
1103	3	0.6140761939348955
1103	4	0.6200075192493912
1103	5	0.7658996339877078
1103	6	0.6715102178770636
1103	7	0.5489484806683151
1103	8	0.6661300428312984
1103	9	0.8517486808652279
1103	10	0.6057483482958617
1103	11	0.873045602081902
1103	12	0.7474578700600816
1103	13	0.876786715919728
1103	14	0.8690009954281805
1103	15	1.0206816675630037
1103	16	0.8959315958184462
1103	17	0.9201690979942754
1103	18	0.9816923517614455
1103	19	0.9288785669307629
1103	20	1.0946784638948373
1103	21	1.2489141866401638
1103	22	1.078046460195651
1103	23	1.190569229854347
1103	24	1.211966204683712
1103	25	1.3703113920667391
1104	1	0.3896421079886494
1104	2	0.5380228324198022
1104	3	0.5282531023648787
1104	4	0.6142582837753849
1104	5	0.6349931658092984
1104	6	0.4943715645968112
1104	7	0.4853531889944378
1104	8	0.4450147003813498
1104	9	0.7236637463548163
1104	10	0.5811213792808019
1104	11	0.6278150902822333
1104	12	0.6646599934228448
1104	13	0.6040736583927497
1104	14	0.8243388293673632
1104	15	0.7703429681226979
1104	16	0.9018256584807318
1104	17	0.7724430579504258
1104	18	0.8622041514267877
1104	19	0.7472975848754828
1104	20	0.7944782446345542
1104	21	0.9188085955028953
1104	22	0.9289281173418047
1104	23	0.8654246911664458
1104	24	0.8280131725447717
1104	25	0.9048200759280292
1105	1	0.3941259816775968
1105	2	0.5423036861160604
1105	3	0.5347683007452169
1105	4	0.49613607811219396
1105	5	0.46269386597520423
1105	6	0.45659409925362127
1105	7	0.3846209673033556
1105	8	0.4649708345657544
1105	9	0.4825794733744715
1105	10	0.529547552550774
1105	11	0.6838795392882275
1105	12	0.47144119860801226
1105	13	0.5543652535900065
1105	14	0.5937739163960174
1105	15	0.5837237290258841
1105	16	0.6743364140412851
1105	17	0.8989515077437531
1105	18	0.8627327907429247
1105	19	0.9086180584013377
1105	20	0.7708851844315348
1105	21	0.8332809403556922
1105	22	0.6539305074663773
1105	23	0.654330014114457
1105	24	0.7362020205047273
1105	25	0.8087972432086303
1106	1	0.48989517332653776
1106	2	0.5427414931890766
1106	3	0.5250281989440175
1106	4	0.47488142917597287
1106	5	0.4956499039538613
1106	6	0.5299345229977988
1106	7	0.5264074412759427
1106	8	0.45046792248537315
1106	9	0.6161272142457506
1106	10	0.7113414117537928
1106	11	0.7241445231247425
1106	12	0.7458743711172602
1106	13	0.7647779862579327
1106	14	0.8761970679878175
1106	15	0.904040409475364
1106	16	0.8363394035118006
1106	17	0.7191532085578984
1106	18	0.9176377777634954
1106	19	0.8636057423134815
1106	20	0.9848158517114243
1106	21	0.7341386442123721
1106	22	0.8436568559342703
1106	23	0.7492766591413655
1106	24	0.6167702410179814
1106	25	0.82501379408491
1107	1	0.2802423031740773
1107	2	0.36121031886525756
1107	3	0.44922198113303935
1107	4	0.15052265159147282
1107	5	0.4250067203527834
1107	6	0.2771334383769002
1107	7	0.1078499359039995
1107	8	0.03064417338070058
1107	9	0.15297192245544267
1107	10	0.20877481671303144
1107	11	0.4851079768578188
1107	12	0.5045824614341257
1107	13	0.4291040205701506
1107	14	0.6583477211478836
1107	15	0.6272275918485661
1107	16	0.4919850599120892
1107	17	0.6486635090456332
1107	18	0.7124210241225128
1107	19	0.7613682853742887
1107	20	0.6953256746931089
1107	21	0.6311508206167531
1107	22	0.7123064517595026
1107	23	0.7701590221141785
1107	24	0.9182300452404988
1107	25	0.8738110757111599
1108	1	0.35630052049907945
1108	2	0.2854574944928494
1108	3	0.28063379840557445
1108	4	0.3951997959009148
1108	5	0.20538102143333958
1108	6	0.21595851585242798
1108	7	0.26356652304552597
1108	8	0.23137650158179213
1108	9	0.25092722440503934
1108	10	0.3095626895383887
1108	11	0.19655570342249007
1108	12	0.06941492572979513
1108	13	-0.05521845799085412
1108	14	0.004661279775135085
1108	15	0.0650852500380168
1108	16	0.059358024642278906
1108	17	0.21800923026491148
1108	18	0.31003608962827656
1108	19	0.29466226605870105
1108	20	0.26145964586729775
1108	21	0.38218382765104053
1108	22	0.3839670105671189
1108	23	0.44169147985107315
1108	24	0.5179849110315686
1108	25	0.4889677894370337
1109	1	0.29062519051719704
1109	2	0.10441164982217899
1109	3	0.2482628546302479
1109	4	0.00975002821184745
1109	5	0.24166750787000524
1109	6	0.18655431121399602
1109	7	0.13306993986468996
1109	8	-0.026539837808250866
1109	9	0.079629017454252
1109	10	0.11173997089114351
1109	11	0.04414959298442511
1109	12	0.22446007198276163
1109	13	0.13829137771713818
1109	14	0.25386327098244266
1109	15	0.21271764474028743
1109	16	0.30084772298112483
1109	17	0.24415688314106632
1109	18	0.07816806804491705
1109	19	0.2914241476082893
1109	20	0.38410671041358313
1109	21	0.02712700696722692
1109	22	0.2360442912030294
1109	23	0.33982797442912016
1109	24	0.3263317194572295
1109	25	0.206722038571435
1110	1	0.0012122985373288486
1110	2	0.10067849613543345
1110	3	-0.06999764207021997
1110	4	0.051427427441339546
1110	5	0.11982485003528609
1110	6	-0.14587520061570558
1110	7	-0.27590039965009083
1110	8	-0.22257867114183938
1110	9	-0.12268271307180356
1110	10	-0.10513908620811918
1110	11	-0.03531354009071919
1110	12	-0.1885670492670002
1110	13	-0.09612049562185376
1110	14	0.042364974729160565
1110	15	-0.023628376133466134
1110	16	0.023945061864402817
1110	17	0.03646794812121357
1110	18	0.07561867158328539
1110	19	0.02373497888528675
1110	20	-0.052082932574379015
1110	21	0.018618180355140623
1110	22	0.016554871604782306
1110	23	0.009030828249329659
1110	24	0.03655861980671915
1110	25	-0.02311433366483584
1111	1	-0.3563956278604081
1111	2	-0.409685308495888
1111	3	-0.3287992792158043
1111	4	-0.4114759819588798
1111	5	-0.29518759682429163
1111	6	-0.46252776249119165
1111	7	-0.4795656657357412
1111	8	-0.5553547414975242
1111	9	-0.6094989400154205
1111	10	-0.6167094691160813
1111	11	-0.5258648147184246
1111	12	-0.4387731771801384
1111	13	-0.536543837319087
1111	14	-0.48359625441678133
1111	15	-0.5949189397807189
1111	16	-0.5707168933071074
1111	17	-0.4626328095782739
1111	18	-0.4236251136477662
1111	19	-0.4372491891052519
1111	20	-0.49423002822466344
1111	21	-0.4670285861833463
1111	22	-0.4978062803359862
1111	23	-0.5795918017745763
1111	24	-0.5569769316906203
1111	25	-0.3553435340469022
1112	1	-0.22217249302125452
1112	2	-0.1498900729306314
1112	3	-0.1685660832536584
1112	4	-0.2866041392225611
1112	5	-0.1828064803364029
1112	6	-0.2637248542427031
1112	7	-0.16862431413730405
1112	8	-0.40286411097815034
1112	9	-0.3956249290161509
1112	10	-0.3851322851775052
1112	11	-0.3003793487812132
1112	12	-0.2692188437654366
1112	13	-0.47030651543494173
1112	14	-0.45310463788820243
1112	15	-0.3101116184230932
1112	16	-0.24624041154109477
1112	17	-0.17882681406796053
1112	18	-0.24753200757518812
1112	19	-0.07341658091946357
1112	20	0.06053225261564421
1112	21	-0.1703254946137207
1112	22	-0.21848758397416645
1112	23	-0.4612778775058217
1112	24	-0.3319351698932067
1112	25	-0.2737570165260905
1113	1	-0.13707359396209995
1113	2	-0.12543735306148918
1113	3	-0.10446835590183312
1113	4	-0.07319934334326206
1113	5	0.024670172524999145
1113	6	-0.15315002158803281
1113	7	-0.10915912312580645
1113	8	-0.20024273486009747
1113	9	-0.018440506923084854
1113	10	-0.010934904697450065
1113	11	0.16296059715783429
1113	12	-0.1682789256689361
1113	13	-0.12917866012340085
1113	14	-0.021711698294936355
1113	15	0.05622720221288091
1113	16	0.07089505406755592
1113	17	0.030594640937316342
1113	18	0.02652778430174152
1113	19	-0.05115460572253028
1113	20	-0.050996651431965745
1113	21	0.00224959326433094
1113	22	-0.09376455934511085
1113	23	-0.2559056574104659
1113	24	0.00996392406379698
1113	25	0.008612848553546826
1114	1	-0.6398082723199572
1114	2	-0.4769427281666303
1114	3	-0.4195400815813528
1114	4	-0.44880085966445193
1114	5	-0.38052034701088644
1114	6	-0.6167898837825447
1114	7	-0.6485159540605991
1114	8	-0.6423190348520293
1114	9	-0.826747089554523
1114	10	-0.6093038224174379
1114	11	-0.450410221724745
1114	12	-0.4808890490716706
1114	13	-0.4030230413237774
1114	14	-0.6878795306239169
1114	15	-0.5825454533079792
1114	16	-0.5549833406598879
1114	17	-0.5156741258418833
1114	18	-0.5286633113440189
1114	19	-0.6668727266825627
1114	20	-0.7504345986372556
1114	21	-0.7053608181933484
1114	22	-0.8452883699662763
1114	23	-0.6877884997061443
1114	24	-0.7830854255081547
1114	25	-0.8047390739251057
1115	1	-0.6502939329451536
1115	2	-0.7064543022140342
1115	3	-0.7214175084442047
1115	4	-0.6467586653771562
1115	5	-0.7393976240851714
1115	6	-0.842641547011759
1115	7	-0.7918709875325831
1115	8	-0.6544800229956849
1115	9	-0.8157286425163051
1115	10	-0.6272060911381274
1115	11	-0.6404292333506376
1115	12	-0.6530246883808033
1115	13	-0.6295176431742462
1115	14	-0.6705252576169656
1115	15	-0.8312304249057618
1115	16	-0.8301218950849929
1115	17	-0.957879935333871
1115	18	-0.7558450970541912
1115	19	-0.8497986735191461
1115	20	-0.7418441090590804
1115	21	-0.6601232880916044
1115	22	-0.7880018474807073
1115	23	-0.7813293862240229
1115	24	-0.6124089572921321
1115	25	-0.498505795514248
1116	1	-1.2081951137215903
1116	2	-1.1864564807683422
1116	3	-1.1385121884145768
1116	4	-1.0459987856333584
1116	5	-1.2332257797210886
1116	6	-1.422084272818914
1116	7	-1.3924326188340572
1116	8	-1.349048451386608
1116	9	-1.3652371606636606
1116	10	-1.501896518570483
1116	11	-1.365679113530156
1116	12	-1.6461374067808734
1116	13	-1.582290507101746
1116	14	-1.7460749068697226
1116	15	-1.8808870847462984
1116	16	-1.7012713003396627
1116	17	-1.708243753903865
1116	18	-1.9632578459540184
1116	19	-2.070044414445462
1116	20	-1.9194524921360738
1116	21	-2.1397028271095095
1116	22	-2.131551583875634
1116	23	-2.138573950731015
1116	24	-2.2323845574838472
1116	25	-2.226976014676815
1201	1	0.6034965969278936
1201	2	0.6098714242248171
1201	3	0.5793635356444682
1201	4	0.5130727564466682
1201	5	0.5808278427015381
1201	6	0.5361290848954425
1201	7	0.5264594100352937
1201	8	0.6458339081899709
1201	9	0.9247194205308844
1201	10	0.9406972504153472
1201	11	0.8490168855674298
1201	12	0.8345096600749855
1201	13	0.7327892160786362
1201	14	0.7419166468466969
1201	15	0.6629082215106408
1201	16	0.7372898036141393
1201	17	0.8021884353815452
1201	18	0.8539423535270956
1201	19	0.829723253386824
1201	20	0.7100336065239574
1201	21	0.7971870288405825
1201	22	0.7225348104439036
1201	23	0.767816371268701
1201	24	0.8395820135981182
1201	25	0.7588901152906757
1202	1	0.5050025484313849
1202	2	0.44841510173218424
1202	3	0.41437456821336127
1202	4	0.47599080657173687
1202	5	0.39670561634551404
1202	6	0.2972178110841668
1202	7	0.194482626397658
1202	8	0.33152327410389404
1202	9	0.5394967451504797
1202	10	0.3728212129677928
1202	11	0.4238482381861175
1202	12	0.4019966912493089
1202	13	0.4091593956181896
1202	14	0.6036059051258036
1202	15	0.38128464386966954
1202	16	0.3995288184279673
1202	17	0.6374989735322387
1202	18	0.49516567003663425
1202	19	0.5486372648663078
1202	20	0.400591108085834
1202	21	0.4243066743008512
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2210	20	-0.12314799822146938
2210	21	-0.1446993248499311
2210	22	-0.09847656440876307
2210	23	-0.093853473712592
2210	24	-0.2621128745858954
2210	25	-0.23737257001784684
2211	1	-0.6690070055163346
2211	2	-0.6636536841914733
2211	3	-0.5745725600816165
2211	4	-0.6763791391170102
2211	5	-0.7752894004353479
2211	6	-0.734409068299847
2211	7	-0.5488956503501612
2211	8	-0.5067382449029623
2211	9	-0.7086913172064229
2211	10	-0.6715262082371396
2211	11	-0.5752668862589445
2211	12	-0.606522905176611
2211	13	-0.561872518233784
2211	14	-0.6795126067416319
2211	15	-0.7405776676190214
2211	16	-0.5621882047235829
2211	17	-0.5384563691578279
2211	18	-0.6223717405643683
2211	19	-0.7508704547484513
2211	20	-0.753185055377088
2211	21	-0.8219996603456465
2211	22	-0.8154685358323732
2211	23	-0.9613193956337653
2211	24	-0.7702296145800389
2211	25	-0.8047954529299375
2212	1	-0.7421679855839827
2212	2	-0.6425334901245563
2212	3	-0.5295241640870323
2212	4	-0.48639212272868093
2212	5	-0.48006640365431963
2212	6	-0.40772078085494456
2212	7	-0.48426178262698016
2212	8	-0.5521576675687427
2212	9	-0.3775158824226068
2212	10	-0.6504673101909972
2212	11	-0.8126036856036336
2212	12	-0.7174236594891568
2212	13	-0.807731321921203
2212	14	-0.9029900042983314
2212	15	-0.8664295429986382
2212	16	-0.8212683743463955
2212	17	-0.9381449667977435
2212	18	-0.7511337838393572
2212	19	-0.8233901484169408
2212	20	-0.851661756388097
2212	21	-0.7408237611980297
2212	22	-0.8408019025998996
2212	23	-0.7948115413845613
2212	24	-0.6496608060818119
2212	25	-0.8900252479969097
2213	1	-0.3474873894143258
2213	2	-0.2863282904302267
2213	3	-0.39775885279694734
2213	4	-0.3670411134114886
2213	5	-0.45680427886964026
2213	6	-0.3838505789915061
2213	7	-0.4184206486630586
2213	8	-0.3815394074678551
2213	9	-0.47652126798885064
2213	10	-0.4925805504794596
2213	11	-0.5575965792700117
2213	12	-0.6871518581107239
2213	13	-0.7097460537229097
2213	14	-0.8012174142348556
2213	15	-0.6672452738619086
2213	16	-0.7100814804948712
2213	17	-0.6622880772844439
2213	18	-0.7550613264637462
2213	19	-0.7596300901867536
2213	20	-0.9485656457217004
2213	21	-0.786077855734959
2213	22	-0.9017271417920156
2213	23	-0.8069113676741821
2213	24	-0.7468410528340428
2213	25	-0.6806148467930981
2214	1	-0.5445535225972478
2214	2	-0.5363093423567626
2214	3	-0.4960517635735542
2214	4	-0.726662768856194
2214	5	-0.5888846892055994
2214	6	-0.4699854363962359
2214	7	-0.6639740205388307
2214	8	-0.6293149917735619
2214	9	-0.7768526389480641
2214	10	-0.6410285710667623
2214	11	-0.7462365482480493
2214	12	-0.8052481495761583
2214	13	-0.6031706315774557
2214	14	-0.683228201417144
2214	15	-0.5851287299905081
2214	16	-0.580746337547076
2214	17	-0.5757769023276254
2214	18	-0.6815520551273337
2214	19	-0.6352893068822141
2214	20	-0.513612619317783
2214	21	-0.641126856334246
2214	22	-0.5636463613076472
2214	23	-0.6777009294889638
2214	24	-0.7592956655882972
2214	25	-0.6799018704473454
2215	1	-0.7436421942436624
2215	2	-0.7439007333587924
2215	3	-0.6359583072381447
2215	4	-0.5912616639028414
2215	5	-0.7801199306774933
2215	6	-0.5006371351329406
2215	7	-0.6998604788363068
2215	8	-0.63976385756132
2215	9	-0.41068624192214287
2215	10	-0.5827405603270321
2215	11	-0.3860097057985641
2215	12	-0.5698694190677429
2215	13	-0.6258178726362829
2215	14	-0.6936581349822575
2215	15	-0.5961163035680102
2215	16	-0.5772369919135372
2215	17	-0.7342331516822114
2215	18	-0.869311082355317
2215	19	-0.9152891347710934
2215	20	-0.9124822795704404
2215	21	-0.9604179306518219
2215	22	-1.09236783389035
2215	23	-1.0714416785165355
2215	24	-1.077498137103909
2215	25	-1.3118913028248897
2216	1	-1.000375349026438
2216	2	-1.0291708926202539
2216	3	-0.9957132271183475
2216	4	-0.8297721344755686
2216	5	-1.0679104379881963
2216	6	-1.090972195118134
2216	7	-0.8969329625707161
2216	8	-0.963185313966882
2216	9	-1.1930356821072934
2216	10	-1.0171659201428724
2216	11	-0.9283491491419227
2216	12	-0.998681709712244
2216	13	-1.015746510438106
2216	14	-1.1744229182472452
2216	15	-1.2382339264417155
2216	16	-1.2788018375137256
2216	17	-1.3620194812082858
2216	18	-1.5117059808160818
2216	19	-1.4760120738757436
2216	20	-1.3333252802476707
2216	21	-1.552855560223243
2216	22	-1.4230378295219097
2216	23	-1.4300398328309711
2216	24	-1.716543347530795
2216	25	-1.7316612594140537
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26808.0
3	1.0
4	207.0
5	1550.0
6	3175.0
7	3229.0
8	2601.0
9	2237.0
10	1999.0
11	2801.0
12	2880.0
13	2600.0
14	1303.0
15	1179.0
16	1312.0
17	1352.0
18	1557.0
19	2127.0
20	2828.0
21	3864.0
22	4548.0
23	5263.0
24	5899.0
25	6541.0
26	7082.0
27	7706.0
28	8331.0
29	9599.0
30	11929.0
31	15061.0
32	19422.0
33	25253.0
34	34297.0
35	48229.0
36	73536.0
37	134138.0
38	420763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.91430479414825	15.996800795142176	13.250853601989633	22.838040808719942
2	11.74029799509432	21.80945367566041	42.094492461532504	24.35575586771277
3	19.30643123174739	30.813231696444888	28.474018027516383	21.40631904429134
4	11.994258067946197	15.349367324153333	35.76889166533751	36.887482942562954
5	15.986431097284779	35.55435701098736	30.766257598729496	17.69295429299837
6	33.562550615585224	34.07737551197214	16.158876713451807	16.201197158990823
7	26.088237901081367	33.60020386456302	22.64780180819004	17.663756426165573
8	25.32400836322177	34.90415202715055	22.526406529251886	17.24543308037579
9	28.798812438378622	18.928868160719627	17.25420687057572	35.01811253032602
10	14.312488784618147	27.25852867482371	30.54095248222708	27.888030058331065
11	31.7233532547102	26.0317753947408	20.60508535583784	21.639785994711158
12	26.027990703175373	24.676342877761456	27.724538694635843	21.571127724427328
13	30.477241299453773	21.85774949285318	27.14120615211955	20.523803055573495
14	28.939077501291393	20.27473237028197	25.415026451256654	25.37116367716998
15	29.437936528614234	24.519533211525633	25.944371515704173	20.09815874415596
16	24.61073338409272	24.76546046333525	30.537448405754514	20.08635774681752
17	22.360568579408643	29.605737001410425	27.429982333473223	20.603712085707702
18	23.910909054477365	22.95545675362177	34.876736300508284	18.256897891392583
19	22.944528317011585	31.73400223561214	22.922261263788872	22.399208183587398
20	20.384071604306055	28.70885145891547	29.4717623877008	21.43531454907767
21	21.964128655882252	31.598623929815968	23.238552231660748	23.19869518264103
22	20.055903794712183	29.45401962977929	22.52727007588534	27.962806499623188
23	23.437980094850044	26.401042014561487	22.658139068866475	27.502838821721998
24	19.20295984969235	28.321149034308966	23.84408697897652	28.631804137022165
25	24.366806102526542	29.341972568461966	23.63215202441723	22.659069304594258
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	461.0
1	461.0
2	668.5
3	876.0
4	876.0
5	876.0
6	2205.0
7	3534.0
8	3534.0
9	3534.0
10	3680.0
11	3826.0
12	3826.0
13	3826.0
14	4510.5
15	5195.0
16	5195.0
17	5195.0
18	8129.0
19	11063.0
20	11063.0
21	11063.0
22	14578.0
23	18093.0
24	18093.0
25	18093.0
26	25833.5
27	33574.0
28	33574.0
29	33574.0
30	40132.0
31	46690.0
32	46690.0
33	46690.0
34	51330.5
35	55971.0
36	55971.0
37	55971.0
38	60740.0
39	65509.0
40	65509.0
41	65509.0
42	73780.0
43	82051.0
44	82051.0
45	82051.0
46	119280.5
47	156510.0
48	156510.0
49	156510.0
50	198364.5
51	240219.0
52	240219.0
53	240219.0
54	155574.5
55	70930.0
56	70930.0
57	70930.0
58	55546.0
59	40162.0
60	40162.0
61	40162.0
62	34131.0
63	28100.0
64	28100.0
65	28100.0
66	23005.0
67	17910.0
68	17910.0
69	17910.0
70	13645.0
71	9380.0
72	9380.0
73	9380.0
74	7654.5
75	5929.0
76	5929.0
77	5929.0
78	4973.5
79	4018.0
80	4018.0
81	4018.0
82	2997.5
83	1977.0
84	1977.0
85	1977.0
86	1431.0
87	885.0
88	885.0
89	885.0
90	599.0
91	313.0
92	313.0
93	313.0
94	168.5
95	24.0
96	24.0
97	24.0
98	15.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19187185218892236
2	0.019043253650602797
3	0.02812201411193669
4	0.041518721621953775
5	0.028786313657887946
6	0.06310845686536973
7	0.07163363437174425
8	0.07573014823844368
9	0.05724047754280026
10	0.04749741753551512
11	0.6472491909385114
12	0.5830335681632228
13	0.5585652015540181
14	0.5481578420007817
15	0.561333116328815
16	0.5370861829015939
17	0.5422898626782122
18	0.5412934133592853
19	0.5556865701882293
20	0.5466078097268954
21	0.553250805186408
22	0.5400755308583747
23	0.5477149756368141
24	0.5295574547141464
25	0.5342075515358052
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	903207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.791585312481715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.69288166436382	42.38773420672289
2	7.670230620980882	7.331449621780349
3	1.4967056790802338	2.1458981144831664
4	0.5638166576661235	1.0778276758179537
5	0.3025680184436907	0.7230102633140093
6	0.19539263465972742	0.5602874261262561
7	0.143190993337155	0.4790327201836151
8	0.10361410733791963	0.3961505960333459
9	0.07429965825016963	0.3195808610356096
>10	0.5513227377200405	5.346181047767027
>50	0.10124311256153472	3.458518948718549
>100	0.08425277743728812	7.970391498975084
>500	0.008844214206083983	3.0528548213735123
>1k	0.00930969916429893	9.651207982442452
>5k	0.0011637123955373662	3.991313472103248
>10k+	0.0011637123955373662	11.108560743122906
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	35860	3.9702969529686993	No Hit
CTTCCGTACTCTGCGTTGATACCAC	21516	2.3821781717812196	No Hit
GAGTACGGAAGCAGTGGTATCAACG	16176	1.790951575884598	No Hit
TCCGTACTCTGCGTTGATACCACTG	14835	1.6424806273644912	No Hit
GCTTCCGTACTCTGCGTTGATACCA	11482	1.2712478977687285	No Hit
GTACATGGAAGCAGTGGTATCAACG	8820	0.9765203325483527	No Hit
GCGTTGATACCACTGCTTCCGTACT	7942	0.8793111656574849	No Hit
GTATCAACGCAGAGTACGGAAGCAG	7164	0.7931736578658048	No Hit
TCCATGTACTCTGCGTTGATACCAC	6636	0.7347152978220939	No Hit
TATCAACGCAGAGTACGGAAGCAGT	5321	0.589122980667776	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4776	0.5287824385772032	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3890	0.43068753895840045	No Hit
GTACAAGCAGTGGTATCAACGCAGA	3873	0.4288053569115386	No Hit
GGTATCAACGCAGAGTACGGAAGCA	3792	0.41983731304119654	No Hit
CAGTGGTATCAACGCAGAGTACGGA	3792	0.41983731304119654	No Hit
CCCATGTACTCTGCGTTGATACCAC	3630	0.40190122530051253	No Hit
ATACCACTGCTTCCGTACTCTGCGT	3470	0.38418657074181223	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	3094	0.3425571325288666	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3006	0.3328140725215814	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2918	0.32307101251429626	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2747	0.30413847545468536	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2690	0.29782762976814836	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2517	0.2786736595265537	No Hit
GTGGTATCAACGCAGAGTACGGAAG	2372	0.26261975383273156	No Hit
CATGTACTCTGCGTTGATACCACTG	2309	0.25564460860024335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2269	0.2512159449605683	No Hit
GCTTGTACTCTGCGTTGATACCACT	2182	0.24158360154427502	No Hit
GTACTCTGCGTTGATACCACTGCTT	2117	0.23438702312980303	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1884	0.20859005742869574	No Hit
GAGTACAAGCAGTGGTATCAACGCA	1861	0.2060435758358826	No Hit
GTTGATACCACTGCTTCCGTACTCT	1823	0.20183634537819126	No Hit
GATACCACTGCTTCCGTACTCTGCG	1739	0.19253615173487362	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	1700	0.18821820468619044	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	1681	0.18611458945734477	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1663	0.184121690819491	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1645	0.18212879218163722	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1620	0.17936087740684029	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1555	0.1721642989923683	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	1452	0.160760490120205	No Hit
GCGTTGATACCACTGCTTCCATGTA	1388	0.1536746282967249	No Hit
GTATCAACGCAGAGTACATGGAAGC	1310	0.1450387341993585	No Hit
GTATCAACGCAGAGTACATGGGAAG	1198	0.1326384760082683	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1182	0.13086701055239827	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1181	0.1307562939614064	No Hit
GTACGGGAAGCAGTGGTATCAACGC	1151	0.1274347962316501	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1078	0.11935248508924311	No Hit
CAGTGGTATCAACGCAGAGTACATG	1063	0.11769173622436496	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1058	0.11713815326940558	No Hit
TATCAACGCAGAGTACATGGAAGCA	1052	0.11647385372345431	No Hit
TGATACCACTGCTTCCGTACTCTGC	1039	0.11503453804055992	No Hit
CTGCTTGTACTCTGCGTTGATACCA	954	0.1056236278062504	No Hit
GCAGTGGTATCAACGCAGAGTACGG	952	0.10540219462426666	No Hit
AAAGTACTCTGCGTTGATACCACTG	945	0.1046271784873235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.1071659099187672E-4	0.0
11	1.1071659099187672E-4	0.0	0.0	5.535829549593837E-4	0.0
12	1.1071659099187672E-4	0.0	0.0	0.0011071659099187673	0.0
13	1.1071659099187672E-4	0.0	0.0	0.0011071659099187673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	40	2.728995E-4	16.649233	7
CCGCCTG	70	2.4812834E-8	16.294924	19
CGAACGT	35	0.002231957	16.216375	4
CGTCGTA	60	1.4430461E-6	15.856414	10
GCCGCCT	60	1.4565194E-6	15.842288	18
CGAGCCG	55	1.1206348E-5	15.555113	15
CCCGTCC	45	6.684163E-4	14.799318	8
CCGTCGT	65	3.3177585E-6	14.636689	9
TCGAACG	40	0.005428312	14.189328	3
CCGACCA	75	9.502237E-7	13.953644	9
TGGTTGA	90	3.6025085E-8	13.730747	14
ACCGTCG	70	7.14604E-6	13.59121	8
TACCGTC	70	7.14604E-6	13.59121	7
AGAACCG	50	0.0014817036	13.320128	5
CGTTCAG	50	0.0014823261	13.319387	10
GTCGCTC	50	0.0014916904	13.308263	16
CGACCAT	80	1.9643285E-6	13.08154	10
GCCAGTA	80	1.9643285E-6	13.08154	11
CGTCTTC	105	1.9490471E-8	12.68513	10
CCGCGGC	100	1.4292164E-7	12.356984	19
>>END_MODULE