FastQCFastQC Report
Thu 2 Feb 2017
SRR4050274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903207
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA311453.448268226442001No Hit
CTTCCGTACTCTGCGTTGATACCAC214292.372545828364926No Hit
GAGTACGGAAGCAGTGGTATCAACG161861.7920587417945166No Hit
TCCGTACTCTGCGTTGATACCACTG152511.688538729217112No Hit
GCTTCCGTACTCTGCGTTGATACCA117151.297044863469836No Hit
GCGTTGATACCACTGCTTCCGTACT81110.8980222695351122No Hit
GTACATGGAAGCAGTGGTATCAACG77440.8573892806410934No Hit
TCCATGTACTCTGCGTTGATACCAC73100.8093382801506189No Hit
GTATCAACGCAGAGTACTTTTTTTT68800.7617301460241118No Hit
GTATCAACGCAGAGTACGGAAGCAG67800.7506584869249242No Hit
TATCAACGCAGAGTACGGAAGCAGT61220.6778069700522693No Hit
TATCAACGCAGAGTACTTTTTTTTT51480.5699690104261814No Hit
ACTCTGCGTTGATACCACTGCTTCC42630.4719848273983705No Hit
CAGTGGTATCAACGCAGAGTACGGA40660.4501736589729708No Hit
CCCATGTACTCTGCGTTGATACCAC40100.44397352987742567No Hit
ACGCAGAGTACGGAAGCAGTGGTAT37090.4106478359888708No Hit
ATACCACTGCTTCCGTACTCTGCGT36900.4085442207600251No Hit
GGTATCAACGCAGAGTACGGAAGCA36650.4057763059852282No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35020.3877295016535523No Hit
GTACAAGCAGTGGTATCAACGCAGA32940.3647004507272419No Hit
AAGCAGTGGTATCAACGCAGAGTAC32520.3600503539055831No Hit
GAAGCAGTGGTATCAACGCAGAGTA29970.33181762320265457No Hit
GGTATCAACGCAGAGTACTTTTTTT28810.31897449864759686No Hit
GTACATGGGAAGCAGTGGTATCAAC27450.3039170422727016No Hit
GTGGTATCAACGCAGAGTACGGAAG26380.2920703670365708No Hit
GAGTACATGGAAGCAGTGGTATCAA26040.288306002942847No Hit
CATGTACTCTGCGTTGATACCACTG25840.2860916711230095No Hit
GCTTGTACTCTGCGTTGATACCACT22000.24357650018212879No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19980.2212117488017697No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19970.22110103221077781No Hit
GTTGATACCACTGCTTCCGTACTCT19840.2196617165278834No Hit
ATCAACGCAGAGTACGGAAGCAGTG19720.21833311743598088No Hit
CATGGAAGCAGTGGTATCAACGCAG19350.21423660356928148No Hit
CCACTGCTTCCGTACTCTGCGTTGA19110.21157940538547643No Hit
GATACCACTGCTTCCGTACTCTGCG18230.20183634537819126No Hit
GAGTACAAGCAGTGGTATCAACGCA18180.20128276242323187No Hit
GTACTCTGCGTTGATACCACTGCTT18100.20039702969529685No Hit
AAAAAGTACTCTGCGTTGATACCAC17760.19663266560157305No Hit
CGGAAGCAGTGGTATCAACGCAGAG17220.19065396968801174No Hit
GCTTCCATGTACTCTGCGTTGATAC16710.185007423547426No Hit
GCGTTGATACCACTGCTTCCATGTA15090.16707133580674197No Hit
CTGCTTCCGTACTCTGCGTTGATAC14820.1640819878499613No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA12990.14382085169844788No Hit
GTATCAACGCAGAGTACATGGAAGC12900.14282440237952096No Hit
GCAGAGTACGGAAGCAGTGGTATCA12780.14149580328761843No Hit
CACTGCTTCCGTACTCTGCGTTGAT12240.1355171073740571No Hit
GTATCAACGCAGAGTACATGGGAAG11050.12234183304602378No Hit
TATCAACGCAGAGTACATGGAAGCA10820.11979535145321063No Hit
GTACGGGAAGCAGTGGTATCAACGC10780.11935248508924311No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10490.1161417039504787No Hit
AAAGTACTCTGCGTTGATACCACTG10490.1161417039504787No Hit
GCAGTGGTATCAACGCAGAGTACGG10240.11337378917568176No Hit
CAGTGGTATCAACGCAGAGTACATG10230.11326307258468987No Hit
CTGCTTGTACTCTGCGTTGATACCA10080.11160232371981173No Hit
CTTGTACTCTGCGTTGATACCACTG9950.11016300803691735No Hit
CCCGTACTCTGCGTTGATACCACTG9700.10739509326212042No Hit
TGATACCACTGCTTCCGTACTCTGC9670.1070629434891448No Hit
TACCACTGCTTCCGTACTCTGCGTT9440.10451646189633162No Hit
CCATGTACTCTGCGTTGATACCACT9390.10396287894137224No Hit
ACGCAGAGTACATGGAAGCAGTGGT9280.1027449964404616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGA453.427096E-516.9507221
CAACACT400.005221138214.2728064
ACTGGTC400.00529851614.2411968
CGGAACC801.2987584E-714.238831514
CATACTC954.782123E-914.0224073
CGACCAT759.739488E-713.92318410
AAACGCT551.9551851E-413.82112319
TATACGC852.7041642E-713.4079337
CGTTATT500.001480742413.3212862
CTTAGGG500.001480742413.3212863
CGCCAGT500.001507579813.28957618
CTAAGAC655.366405E-513.1748983
CCGTCGT655.4820768E-513.1449929
CGTCGTA655.4849254E-513.14426410
CGACGGG655.4877753E-513.14353714
CCTTAGG603.9649048E-412.7130411
TAGACAG1051.9879735E-812.6672575
ATATACG905.411639E-712.663756
TACGCTA905.4407974E-712.658149
CGGTCCA751.4880656E-512.6574410
Produced by FastQC (version 0.11.5)