##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050274_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	903207
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.946647889132834	31.0	31.0	34.0	27.0	34.0
2	30.755134758698727	31.0	31.0	34.0	27.0	34.0
3	31.114838569674504	33.0	31.0	34.0	27.0	34.0
4	34.63931856152576	37.0	35.0	37.0	32.0	37.0
5	34.54486513058468	37.0	35.0	37.0	32.0	37.0
6	34.53355100215122	37.0	35.0	37.0	31.0	37.0
7	34.40385648029743	37.0	35.0	37.0	31.0	37.0
8	34.406611109081304	37.0	35.0	37.0	31.0	37.0
9	35.958057233834545	39.0	37.0	39.0	31.0	39.0
10	35.92902180784693	39.0	37.0	39.0	31.0	39.0
11	35.91554538439139	39.0	37.0	39.0	31.0	39.0
12	35.85668512312238	39.0	37.0	39.0	31.0	39.0
13	35.806156285325514	39.0	37.0	39.0	30.0	39.0
14	37.017622759788175	40.0	38.0	41.0	31.0	41.0
15	37.02852391533724	40.0	37.0	41.0	31.0	41.0
16	36.95551628807129	40.0	37.0	41.0	31.0	41.0
17	36.9807342060015	40.0	37.0	41.0	31.0	41.0
18	36.92426874459564	40.0	37.0	41.0	31.0	41.0
19	37.0381640089149	40.0	38.0	41.0	31.0	41.0
20	36.993902837334076	40.0	38.0	41.0	31.0	41.0
21	36.96672745007512	40.0	37.0	41.0	31.0	41.0
22	36.91192052320232	40.0	37.0	41.0	31.0	41.0
23	36.83924172421162	40.0	37.0	41.0	31.0	41.0
24	36.7735281059602	40.0	37.0	41.0	31.0	41.0
25	36.579632354487956	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3373005572797325
1101	2	0.6845774393202078
1101	3	0.40870038580728263
1101	4	0.3622996244967567
1101	5	0.4824012909573554
1101	6	0.44556821003153857
1101	7	0.39744469822756656
1101	8	0.44528619749416976
1101	9	0.542956771658254
1101	10	0.48243651165456214
1101	11	0.4444874042129996
1101	12	0.46929711790372863
1101	13	0.4682028842389556
1101	14	0.6761452810401352
1101	15	0.6517280127396674
1101	16	0.5539591802725354
1101	17	0.5799844262425751
1101	18	0.5242462386365361
1101	19	0.6000697551998684
1101	20	0.5787613865560459
1101	21	0.2680770068600751
1101	22	0.4759844159562192
1101	23	0.5292311833638124
1101	24	0.5734648087091117
1101	25	0.4617041087531035
1102	1	0.312683326693584
1102	2	0.6865819536265754
1102	3	0.5917357159097882
1102	4	0.3731563346099378
1102	5	0.4136933513349703
1102	6	0.4234792123009825
1102	7	0.39081092993737343
1102	8	0.34826303339814757
1102	9	0.36446955604603204
1102	10	0.3486288810998843
1102	11	0.32221018744515106
1102	12	0.3362209721771947
1102	13	0.4350604745926958
1102	14	0.43786809377755986
1102	15	0.42777256078039017
1102	16	0.48636199114129397
1102	17	0.554344346283294
1102	18	0.6164680346715201
1102	19	0.523697206903428
1102	20	0.5446901755859841
1102	21	0.48225822331320245
1102	22	0.669561733287857
1102	23	0.5970478179434977
1102	24	0.6750004350937076
1102	25	0.6628587483339388
1103	1	0.2558583245500543
1103	2	0.5818847630809785
1103	3	0.5424345069584042
1103	4	0.5147794161498496
1103	5	0.5367248755282858
1103	6	0.4670714841578558
1103	7	0.328627100733911
1103	8	0.4203857005221039
1103	9	0.5107440994739676
1103	10	0.37935282291017103
1103	11	0.345946218769285
1103	12	0.4344589898398894
1103	13	0.5596424860820193
1103	14	0.6408033019858479
1103	15	0.4771952364784724
1103	16	0.5825350741102326
1103	17	0.6057176350311835
1103	18	0.5733973553151515
1103	19	0.5174309517946796
1103	20	0.577293279418619
1103	21	0.6342559940411405
1103	22	0.5570549454402496
1103	23	0.6968934837241676
1103	24	0.8372371380574961
1103	25	0.7169471761255295
1104	1	0.4692741689906512
1104	2	0.7783523644888604
1104	3	0.5558261173674985
1104	4	0.5980608194156716
1104	5	0.6845403506884082
1104	6	0.5736919907645088
1104	7	0.6408173955812941
1104	8	0.5281936746724654
1104	9	0.6740422357615401
1104	10	0.7355365399490026
1104	11	0.8053207499561736
1104	12	0.7889665196996773
1104	13	0.6538995976162667
1104	14	0.8948809662868129
1104	15	0.8850505246903424
1104	16	1.0336440695519258
1104	17	0.9443143692069427
1104	18	0.99194619842865
1104	19	0.8897649198288349
1104	20	1.1080687684267545
1104	21	0.9842813820741441
1104	22	0.9654889654044752
1104	23	0.906875596700921
1104	24	1.0356428849849664
1104	25	0.8935082516549286
1105	1	0.3337659160311155
1105	2	0.8133595348625633
1105	3	0.6523359731164504
1105	4	0.5667136327328848
1105	5	0.4575229449577307
1105	6	0.46077069616057287
1105	7	0.6857837932417823
1105	8	0.7092762605060585
1105	9	0.7713551733652295
1105	10	0.898642577122466
1105	11	0.7351731246152298
1105	12	0.7507368990265064
1105	13	0.712236633299419
1105	14	0.7542949639810459
1105	15	0.741589061934107
1105	16	0.7721318236784
1105	17	0.8838706496107349
1105	18	0.8693395566942641
1105	19	0.8391038394925303
1105	20	0.7662314656120301
1105	21	0.8112807915727842
1105	22	0.9440520787526197
1105	23	0.9654870749226632
1105	24	0.9816300064314092
1105	25	0.9106035473273906
1106	1	0.4280105219384147
1106	2	0.7905504497024545
1106	3	0.47430548582391197
1106	4	0.5121137839753303
1106	5	0.36310034173033756
1106	6	0.35971796915318066
1106	7	0.3112630593179304
1106	8	0.380580439131343
1106	9	0.6121492846389813
1106	10	0.4563181311253075
1106	11	0.45385554644013126
1106	12	0.5978338843489155
1106	13	0.5464912778133453
1106	14	0.686758800674589
1106	15	0.6488800975026194
1106	16	0.6528691969244136
1106	17	0.7802518867031623
1106	18	0.5992347041114243
1106	19	0.7377006457120459
1106	20	0.8041261000431206
1106	21	0.7348558179959852
1106	22	0.822808264435885
1106	23	0.9179640016054549
1106	24	0.9432113302007963
1106	25	0.7983635229163326
1107	1	0.16687013515851135
1107	2	0.37354483900631763
1107	3	0.2324060349558721
1107	4	0.19147060037425234
1107	5	0.12537693711217202
1107	6	0.1994017066612841
1107	7	0.19360258317617962
1107	8	0.17693689740072926
1107	9	0.2617195291903016
1107	10	0.2436296867718184
1107	11	0.20203079116198808
1107	12	0.22467569341471005
1107	13	0.1543778019103499
1107	14	0.26723349285371967
1107	15	0.22978539781981766
1107	16	0.2407260474309112
1107	17	0.29456214626181065
1107	18	0.12615623963446154
1107	19	0.23031848738467886
1107	20	0.42500761032655987
1107	21	0.2381194866067844
1107	22	0.09613145076556862
1107	23	0.04151016620861725
1107	24	0.1720731604132908
1107	25	0.25938495952740226
1108	1	-0.016863693362321186
1108	2	0.25168587726037117
1108	3	0.0811155960098695
1108	4	-0.06453170339540293
1108	5	0.17947965572433588
1108	6	-0.02581329817581235
1108	7	-0.0685239765824619
1108	8	-0.0680558879469686
1108	9	-0.10745750853904923
1108	10	-0.13954111192298768
1108	11	-0.06879331889425089
1108	12	0.0045652600436625335
1108	13	0.2006479086544104
1108	14	-0.023442183929859084
1108	15	0.2104072926498617
1108	16	0.20960671052304036
1108	17	0.2948685640229698
1108	18	0.22025723656823715
1108	19	0.15514160542623046
1108	20	0.13557502633324958
1108	21	0.3410891768686426
1108	22	0.13637260871726653
1108	23	0.1811710775503812
1108	24	0.24276855451367396
1108	25	0.12244696454486359
1109	1	-0.23166502834373048
1109	2	0.10420914163605133
1109	3	0.0461813978128518
1109	4	0.025001452500234222
1109	5	-0.04168321999519975
1109	6	-0.04953953072433137
1109	7	-0.0868354237715323
1109	8	0.05515083242890739
1109	9	0.0023809644453933743
1109	10	8.295819785573144E-4
1109	11	-0.08524095343950933
1109	12	0.03001705539703181
1109	13	0.020843752352213585
1109	14	-0.02878426011473323
1109	15	-0.05400269287999038
1109	16	0.05460764754973013
1109	17	-0.007919576803431028
1109	18	-0.2062967684271939
1109	19	-0.10732673792082892
1109	20	-0.09220592038116848
1109	21	-0.0872671963977254
1109	22	-0.008002210149847144
1109	23	0.13489168793877582
1109	24	0.05722139749359911
1109	25	0.13167590935041318
1110	1	-0.10938544724137245
1110	2	0.22430410640022203
1110	3	0.004076728271634522
1110	4	0.09258738539999456
1110	5	0.05530561661531408
1110	6	0.08172441208139247
1110	7	-0.0432360702147534
1110	8	0.05336452753470411
1110	9	-0.007516871717527351
1110	10	-0.013009708498699979
1110	11	-0.04748113015973132
1110	12	0.004329523612135233
1110	13	0.0565210774063587
1110	14	0.14026440043602406
1110	15	0.09273443020383354
1110	16	0.10235269164595451
1110	17	0.23193097511702376
1110	18	0.2632773685604448
1110	19	0.060371016852435844
1110	20	-0.0031929656062814615
1110	21	0.12025916252254376
1110	22	0.23822488122655727
1110	23	0.2394504741862633
1110	24	0.2316919709655494
1110	25	0.20215010826937885
1111	1	-0.1317734626230198
1111	2	0.1651421784484839
1111	3	0.005402578642073053
1111	4	0.11673082865171835
1111	5	-0.19082051486113727
1111	6	0.07390837815071194
1111	7	-0.057240395684047485
1111	8	-0.07384881114360553
1111	9	-0.13885920242291405
1111	10	-0.11188132520471328
1111	11	-0.17030664152026986
1111	12	-0.043897974296889686
1111	13	0.014174040154863121
1111	14	0.0047133980060394265
1111	15	0.015072955090133178
1111	16	-0.1075196723665357
1111	17	0.02073869959353658
1111	18	0.009325799959192693
1111	19	0.03667348296381334
1111	20	0.014391502296653869
1111	21	0.13740712425649093
1111	22	0.2043753620267239
1111	23	0.10518671954034176
1111	24	-0.012236782517341283
1111	25	0.045293447591063796
1112	1	-0.08296169943753995
1112	2	0.06776850489411146
1112	3	0.05899505050969722
1112	4	0.07681277271723985
1112	5	-0.18353553427020586
1112	6	-0.13084121859067466
1112	7	-0.14921734412916265
1112	8	-0.08775249814790698
1112	9	-0.032493220697588754
1112	10	-0.3458416838057303
1112	11	-0.19543551675181448
1112	12	-0.14288372502802105
1112	13	-0.10222195262716838
1112	14	-0.1340932436753448
1112	15	-0.05417073756218116
1112	16	-0.15841306371167008
1112	17	-0.22264235467047655
1112	18	-0.05206811071602857
1112	19	-0.15465490117770742
1112	20	-0.09382396464570775
1112	21	-0.09123835757173993
1112	22	-0.16260577456426972
1112	23	-0.0881601115888273
1112	24	-0.009451067205958452
1112	25	0.033504893913395506
1113	1	-0.1736295726147219
1113	2	-0.0063358350634281635
1113	3	0.09199496930225948
1113	4	-0.026832049148445947
1113	5	0.06888317128322541
1113	6	0.03510548612747755
1113	7	0.050577549083051565
1113	8	1.3483233156819097E-4
1113	9	-0.038935058769958175
1113	10	0.011558286031508658
1113	11	0.033168527378627743
1113	12	0.008243782251646792
1113	13	-0.03279052070800503
1113	14	-0.15600486678002312
1113	15	-0.2599063029399744
1113	16	-0.2642388853569386
1113	17	-0.0794272361656212
1113	18	-0.14359585465737723
1113	19	0.05051963680996607
1113	20	-0.043271174010087066
1113	21	-0.04722485393480724
1113	22	0.07658586338976647
1113	23	0.07315637700152422
1113	24	-0.17100885789958653
1113	25	0.010169588399406848
1114	1	-0.4113753698931788
1114	2	-0.6418089941043199
1114	3	-0.3131158166268335
1114	4	-0.2506982073021291
1114	5	-0.20464964969639254
1114	6	-0.13386569685653882
1114	7	-0.10983817180323285
1114	8	-0.12638811412477935
1114	9	-0.1906469116545395
1114	10	-0.09452336026006947
1114	11	-0.2251572973226601
1114	12	-0.26315512774846894
1114	13	-0.33713922849355527
1114	14	-0.4050069292533749
1114	15	-0.44120577637554703
1114	16	-0.48515536094716083
1114	17	-0.5689362753295697
1114	18	-0.36361682505685167
1114	19	-0.4174341361336076
1114	20	-0.5299872324967652
1114	21	-0.4572090685242145
1114	22	-0.5616503186148663
1114	23	-0.5510416204405502
1114	24	-0.5105182427294608
1114	25	-0.49452248087204254
1115	1	-0.8156634709198052
1115	2	-1.0083697333685109
1115	3	-0.7086829967994355
1115	4	-0.5163235745721764
1115	5	-0.5223032693947403
1115	6	-0.8279453613301442
1115	7	-0.6199309254932146
1115	8	-0.6635939812277556
1115	9	-0.888426584193688
1115	10	-0.7084417139684902
1115	11	-0.7824246929603547
1115	12	-0.7239596075788626
1115	13	-0.6382142495215675
1115	14	-0.6848184261020549
1115	15	-0.7453300317535323
1115	16	-0.96932363111965
1115	17	-0.8218001175215548
1115	18	-0.934104329233648
1115	19	-0.876260024206978
1115	20	-0.8968304960439824
1115	21	-1.0363773963076923
1115	22	-0.919346340000061
1115	23	-0.8827758263883041
1115	24	-1.0855851290860272
1115	25	-1.1315253268548275
1116	1	-0.822946813693541
1116	2	-1.1555546054873744
1116	3	-0.870672888307432
1116	4	-0.4924688405736575
1116	5	-0.5804244841566231
1116	6	-0.6978499406513095
1116	7	-0.7656670589950281
1116	8	-0.6085837420224891
1116	9	-0.754613242727082
1116	10	-0.6587928969234866
1116	11	-0.9379464567179312
1116	12	-1.0067864996532805
1116	13	-0.97037842537447
1116	14	-0.9910233844916689
1116	15	-1.1403668493213956
1116	16	-1.1494543443783272
1116	17	-1.361321271283913
1116	18	-1.190395558373993
1116	19	-1.1467554274573857
1116	20	-1.129918814635765
1116	21	-0.9946816096317264
1116	22	-1.2692561478515714
1116	23	-1.1916547423396793
1116	24	-1.3055071368416833
1116	25	-1.2100160244279223
1201	1	0.42001247276568776
1201	2	0.6077020819783634
1201	3	0.4819945778668675
1201	4	0.21141797368528614
1201	5	0.23428863311175263
1201	6	0.31956210856194645
1201	7	0.2732510528212586
1201	8	0.23333638241572174
1201	9	0.32103571405399123
1201	10	0.24941165433306622
1201	11	0.28114337113066057
1201	12	0.2799735352559125
1201	13	0.32928356496415745
1201	14	0.3856352475191116
1201	15	0.5219049991046276
1201	16	0.3334532115624711
1201	17	0.41943107726974915
1201	18	0.338592530149711
1201	19	0.37165897476224075
1201	20	0.5239265400072171
1201	21	0.35630223991834953
1201	22	0.4197739741920756
1201	23	0.39756654910262057
1201	24	0.37294385670486463
1201	25	0.37607452179015155
1202	1	0.5187855528296126
1202	2	0.6668819280605724
1202	3	0.5942828473095751
1202	4	0.46940049940649686
1202	5	0.34218902878670576
1202	6	0.3634677549441321
1202	7	0.33069294756845125
1202	8	0.3480973278238153
1202	9	0.22878273011263417
1202	10	0.2814423723743005
1202	11	0.2338583424319438
1202	12	0.29999536640496416
1202	13	0.28202427502637306
1202	14	0.3711905378874363
1202	15	0.416014566670043
1202	16	0.5081901497262677
1202	17	0.43524228111839136
1202	18	0.36866008269772976
1202	19	0.3730424331625528
1202	20	0.5916961125799887
1202	21	0.5276615506811311
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2210	19	-0.3162576799258545
2210	20	-0.36464398503758844
2210	21	-0.178365346702698
2210	22	-0.3327359360804252
2210	23	-0.14359022965154367
2210	24	-0.2147728356758023
2210	25	-0.0770812731239019
2211	1	-0.5813720477253739
2211	2	-1.022870494279747
2211	3	-0.6843394134302834
2211	4	-0.5787364182891395
2211	5	-0.5277358271020134
2211	6	-0.5839667830143753
2211	7	-0.6066439051286778
2211	8	-0.6883237125273425
2211	9	-0.6747627094710396
2211	10	-0.543057482837348
2211	11	-0.6487979925270437
2211	12	-0.6119832132525858
2211	13	-0.5631454190014722
2211	14	-0.6338171598528248
2211	15	-0.5544684014286076
2211	16	-0.6376950487379389
2211	17	-0.6183382618274962
2211	18	-0.6655995488523772
2211	19	-0.6050252569528851
2211	20	-0.5270527285924018
2211	21	-0.592356532237801
2211	22	-0.6179869227070469
2211	23	-0.6120944913666406
2211	24	-0.7005998245472824
2211	25	-0.7578963030284314
2212	1	-0.2466776641125854
2212	2	-0.8361493484527998
2212	3	-0.48271404727943334
2212	4	-0.20208490845669758
2212	5	-0.13752489449234417
2212	6	-0.3216769308492289
2212	7	-0.1367578971527621
2212	8	-0.15126841051441886
2212	9	-0.14798517130498112
2212	10	-0.2668891899686727
2212	11	-0.22360699969868136
2212	12	-0.19700218715720297
2212	13	-0.12549090194910661
2212	14	-0.2548730859706865
2212	15	-0.3275398521568178
2212	16	-0.30540281903017075
2212	17	-1.206787026712675
2212	18	-0.542537106781765
2212	19	-0.5993177615640164
2212	20	-0.5934135652645054
2212	21	-0.33108549423612743
2212	22	-0.3434945187699867
2212	23	-0.40784036177942085
2212	24	-0.37402066259390665
2212	25	-0.29243846818962993
2213	1	-0.3717151016939262
2213	2	-0.7360648529661837
2213	3	-0.4276762648185546
2213	4	-0.31492177862044457
2213	5	-0.43737904675121797
2213	6	-0.3312677585056676
2213	7	-0.38755248145493937
2213	8	-0.3009243477971708
2213	9	-0.45637872242066635
2213	10	-0.49734495735287254
2213	11	-0.4323453395938017
2213	12	-0.4143783980927864
2213	13	-0.3736235607706675
2213	14	-0.6615178255594145
2213	15	-0.569945241716411
2213	16	-0.5051252817604919
2213	17	-1.3310648217344365
2213	18	-1.2601874061916547
2213	19	-0.912561408066118
2213	20	-0.7225632375381892
2213	21	-0.5325627585064225
2213	22	-0.6840943731945188
2213	23	-0.7246476122363887
2213	24	-0.6362584406984126
2213	25	-0.7364418298575401
2214	1	-0.7465599918832311
2214	2	-2.1143710714595016
2214	3	-1.033776663168748
2214	4	-0.61584186181571
2214	5	-0.5821850713572729
2214	6	-0.5053472346575347
2214	7	-0.44075162130053513
2214	8	-0.49013055571482056
2214	9	-0.7975590552017167
2214	10	-0.7032320797133593
2214	11	-0.6122462808283302
2214	12	-0.7611228546796625
2214	13	-0.605921653625046
2214	14	-0.8091806027300663
2214	15	-0.6475512627972435
2214	16	-0.6393102502097463
2214	17	-0.7118201888864064
2214	18	-0.8335155691979637
2214	19	-0.743690889505821
2214	20	-0.5593617181670894
2214	21	-0.7347404649963565
2214	22	-0.6423213623015798
2214	23	-0.6517142742027602
2214	24	-0.6894589025026221
2214	25	-0.7741903580769502
2215	1	-0.8251829308526304
2215	2	-2.1106237822095615
2215	3	-1.098722792389772
2215	4	-0.8027338793021954
2215	5	-0.6777149154246018
2215	6	-0.738593508451892
2215	7	-0.8463383480913507
2215	8	-0.6705283905569956
2215	9	-0.7830358890456424
2215	10	-0.7584728631631563
2215	11	-0.8707141476831737
2215	12	-0.9701242659908047
2215	13	-0.8408946321799959
2215	14	-0.9710425007714392
2215	15	-1.1556972818671554
2215	16	-1.0666784991512586
2215	17	-1.0520137278351527
2215	18	-1.021433368929472
2215	19	-1.147413652921884
2215	20	-1.1652784680207944
2215	21	-1.1380217634026124
2215	22	-1.1432223639882721
2215	23	-1.3777030525580614
2215	24	-1.3808537770336216
2215	25	-1.2665292620733695
2216	1	-1.0775302406991862
2216	2	-2.2730587840870804
2216	3	-1.2273094495072172
2216	4	-0.943398395736061
2216	5	-0.8295993532597521
2216	6	-0.9610398168735301
2216	7	-0.9381634017021057
2216	8	-0.9197208098673144
2216	9	-0.974721185813415
2216	10	-1.1359783941687027
2216	11	-1.0467236339068435
2216	12	-1.0304978129879814
2216	13	-1.0676787078575813
2216	14	-1.1748500043710521
2216	15	-1.1263151878189248
2216	16	-1.2668184217793552
2216	17	-1.1569014042823085
2216	18	-1.116498692405223
2216	19	-0.998580489932742
2216	20	-1.16560796591574
2216	21	-1.2625388106704278
2216	22	-1.2548338698060846
2216	23	-1.195520557973019
2216	24	-1.3322754257499057
2216	25	-1.0759505808772971
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13611.0
3	0.0
4	175.0
5	1919.0
6	3363.0
7	2129.0
8	1647.0
9	1112.0
10	1143.0
11	1122.0
12	999.0
13	810.0
14	795.0
15	836.0
16	815.0
17	925.0
18	1135.0
19	1770.0
20	2468.0
21	2988.0
22	3493.0
23	4022.0
24	4790.0
25	5309.0
26	5718.0
27	6459.0
28	7409.0
29	8613.0
30	11282.0
31	14562.0
32	19307.0
33	25819.0
34	36160.0
35	51537.0
36	78893.0
37	143550.0
38	436522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30527263893727	17.12803459039269	14.132322893950452	23.43436987671959
2	11.707766460309568	22.949596236188267	40.20375871831979	25.138878585182372
3	19.483794966159472	29.797709716598742	28.926480862083665	21.79201445515812
4	12.522915074764418	16.318749383108997	35.28616533898784	35.872170203138744
5	15.32350973276439	35.932798950731346	31.15071716272083	17.592974153783437
6	33.209401671526436	34.97166115695155	15.599214754810292	16.21972241671172
7	25.114697444645053	33.53928290559475	23.078388586745827	18.267631063014367
8	25.018023555208558	35.17622108894389	22.77701179975304	17.028743556094508
9	27.42011390074048	19.060822729653502	17.78162639095438	35.73743697865164
10	13.959462526920714	27.755822901845278	31.15018574600134	27.134528825232668
11	31.771818992198703	25.430566244580895	20.809695976435545	21.987918786784856
12	25.103233372201572	25.2407671327533	28.167955076734312	21.488044418310814
13	30.93041255091974	21.35323876665445	27.184137068905383	20.532211613520428
14	28.054256055363318	20.26153633217993	26.0558615916955	25.628346020761246
15	29.034211691762625	24.394043401240033	26.228631532329494	20.343113374667848
16	24.572632539271304	24.420839588841503	30.451260180424356	20.555267691462838
17	22.411141631984695	28.944749099597324	28.367694227347663	20.27641504107032
18	23.982099341568432	22.315150347594738	35.30121002162295	18.40154028921388
19	22.916009286907652	31.48115548263023	23.871604183758542	21.73123104670358
20	20.386047036888595	27.832521041711416	30.4681324083321	21.313299513067893
21	21.95037965938054	30.678559486612826	24.294214429492303	23.076846424514326
22	20.024359187288933	28.91313735259924	23.673365443171125	27.389138016940706
23	22.85353032094632	25.641161908051778	23.962360662707425	27.542947108294474
24	19.27818626501195	27.97639939016626	25.004179597585026	27.74123474723676
25	24.282004014946864	27.992448973483835	24.870722479822728	22.85482453174657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1504.0
1	1504.0
2	1425.5
3	1347.0
4	1347.0
5	1347.0
6	2835.0
7	4323.0
8	4323.0
9	4323.0
10	4239.0
11	4155.0
12	4155.0
13	4155.0
14	4274.0
15	4393.0
16	4393.0
17	4393.0
18	7179.5
19	9966.0
20	9966.0
21	9966.0
22	14279.5
23	18593.0
24	18593.0
25	18593.0
26	27396.5
27	36200.0
28	36200.0
29	36200.0
30	42286.5
31	48373.0
32	48373.0
33	48373.0
34	52714.0
35	57055.0
36	57055.0
37	57055.0
38	60660.0
39	64265.0
40	64265.0
41	64265.0
42	72075.5
43	79886.0
44	79886.0
45	79886.0
46	117597.0
47	155308.0
48	155308.0
49	155308.0
50	196652.0
51	237996.0
52	237996.0
53	237996.0
54	154920.0
55	71844.0
56	71844.0
57	71844.0
58	55972.0
59	40100.0
60	40100.0
61	40100.0
62	33929.0
63	27758.0
64	27758.0
65	27758.0
66	22729.0
67	17700.0
68	17700.0
69	17700.0
70	13581.5
71	9463.0
72	9463.0
73	9463.0
74	7643.5
75	5824.0
76	5824.0
77	5824.0
78	4946.0
79	4068.0
80	4068.0
81	4068.0
82	2977.0
83	1886.0
84	1886.0
85	1886.0
86	1369.0
87	852.0
88	852.0
89	852.0
90	580.5
91	309.0
92	309.0
93	309.0
94	170.5
95	32.0
96	32.0
97	32.0
98	19.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18655745582131228
2	0.008857327279350138
3	0.0
4	0.1669606192157501
5	0.053476113449076455
6	0.06189057436445909
7	0.04395448662377506
8	0.02457908320019663
9	0.015500322738862742
10	0.010185926371252658
11	0.016828921830765262
12	0.017271788194732768
13	0.007750161369431371
14	0.009078760461333891
15	7.750161369431371E-4
16	3.321497729756302E-4
17	0.0
18	0.0
19	0.0
20	1.1071659099187672E-4
21	0.003210781138764425
22	0.006310845686536974
23	3.321497729756302E-4
24	0.0011071659099187673
25	0.11935248508924311
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	903207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.81447783973561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.59795305372525	41.47666909879556
2	7.7729658689297585	7.277746768400668
3	1.460834397812516	2.0516459863175265
4	0.586079629228505	1.0974804725935325
5	0.310443546344165	0.7266626260408923
6	0.2003443110663125	0.562740858643859
7	0.12367571226308564	0.4052869723737618
8	0.10421928890456376	0.39031772727165626
9	0.08005213700671772	0.3372839094532019
>10	0.5625999376978006	5.27945821315836
>50	0.09481758112479946	3.1929496228017844
>100	0.08409692398097794	7.902904560019941
>500	0.009052926660812217	3.107739705963625
>1k	0.010005866309318765	10.150983126419925
>5k	0.001667644384886461	5.363959847777518
>10k+	0.0011911745606331863	10.676170503968201
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	31145	3.448268226442001	No Hit
CTTCCGTACTCTGCGTTGATACCAC	21429	2.372545828364926	No Hit
GAGTACGGAAGCAGTGGTATCAACG	16186	1.7920587417945166	No Hit
TCCGTACTCTGCGTTGATACCACTG	15251	1.688538729217112	No Hit
GCTTCCGTACTCTGCGTTGATACCA	11715	1.297044863469836	No Hit
GCGTTGATACCACTGCTTCCGTACT	8111	0.8980222695351122	No Hit
GTACATGGAAGCAGTGGTATCAACG	7744	0.8573892806410934	No Hit
TCCATGTACTCTGCGTTGATACCAC	7310	0.8093382801506189	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6880	0.7617301460241118	No Hit
GTATCAACGCAGAGTACGGAAGCAG	6780	0.7506584869249242	No Hit
TATCAACGCAGAGTACGGAAGCAGT	6122	0.6778069700522693	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5148	0.5699690104261814	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4263	0.4719848273983705	No Hit
CAGTGGTATCAACGCAGAGTACGGA	4066	0.4501736589729708	No Hit
CCCATGTACTCTGCGTTGATACCAC	4010	0.44397352987742567	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	3709	0.4106478359888708	No Hit
ATACCACTGCTTCCGTACTCTGCGT	3690	0.4085442207600251	No Hit
GGTATCAACGCAGAGTACGGAAGCA	3665	0.4057763059852282	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3502	0.3877295016535523	No Hit
GTACAAGCAGTGGTATCAACGCAGA	3294	0.3647004507272419	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	3252	0.3600503539055831	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2997	0.33181762320265457	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2881	0.31897449864759686	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2745	0.3039170422727016	No Hit
GTGGTATCAACGCAGAGTACGGAAG	2638	0.2920703670365708	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2604	0.288306002942847	No Hit
CATGTACTCTGCGTTGATACCACTG	2584	0.2860916711230095	No Hit
GCTTGTACTCTGCGTTGATACCACT	2200	0.24357650018212879	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1998	0.2212117488017697	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1997	0.22110103221077781	No Hit
GTTGATACCACTGCTTCCGTACTCT	1984	0.2196617165278834	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	1972	0.21833311743598088	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1935	0.21423660356928148	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	1911	0.21157940538547643	No Hit
GATACCACTGCTTCCGTACTCTGCG	1823	0.20183634537819126	No Hit
GAGTACAAGCAGTGGTATCAACGCA	1818	0.20128276242323187	No Hit
GTACTCTGCGTTGATACCACTGCTT	1810	0.20039702969529685	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1776	0.19663266560157305	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1722	0.19065396968801174	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1671	0.185007423547426	No Hit
GCGTTGATACCACTGCTTCCATGTA	1509	0.16707133580674197	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	1482	0.1640819878499613	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1299	0.14382085169844788	No Hit
GTATCAACGCAGAGTACATGGAAGC	1290	0.14282440237952096	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1278	0.14149580328761843	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1224	0.1355171073740571	No Hit
GTATCAACGCAGAGTACATGGGAAG	1105	0.12234183304602378	No Hit
TATCAACGCAGAGTACATGGAAGCA	1082	0.11979535145321063	No Hit
GTACGGGAAGCAGTGGTATCAACGC	1078	0.11935248508924311	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1049	0.1161417039504787	No Hit
AAAGTACTCTGCGTTGATACCACTG	1049	0.1161417039504787	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1024	0.11337378917568176	No Hit
CAGTGGTATCAACGCAGAGTACATG	1023	0.11326307258468987	No Hit
CTGCTTGTACTCTGCGTTGATACCA	1008	0.11160232371981173	No Hit
CTTGTACTCTGCGTTGATACCACTG	995	0.11016300803691735	No Hit
CCCGTACTCTGCGTTGATACCACTG	970	0.10739509326212042	No Hit
TGATACCACTGCTTCCGTACTCTGC	967	0.1070629434891448	No Hit
TACCACTGCTTCCGTACTCTGCGTT	944	0.10451646189633162	No Hit
CCATGTACTCTGCGTTGATACCACT	939	0.10396287894137224	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	928	0.1027449964404616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1071659099187672E-4	0.0
4	0.0	0.0	0.0	1.1071659099187672E-4	0.0
5	0.0	0.0	0.0	1.1071659099187672E-4	0.0
6	0.0	0.0	0.0	1.1071659099187672E-4	1.1071659099187672E-4
7	0.0	0.0	0.0	1.1071659099187672E-4	1.1071659099187672E-4
8	0.0	0.0	0.0	1.1071659099187672E-4	1.1071659099187672E-4
9	0.0	0.0	0.0	1.1071659099187672E-4	1.1071659099187672E-4
10	0.0	0.0	0.0	3.321497729756302E-4	1.1071659099187672E-4
11	0.0	0.0	0.0	7.750161369431371E-4	1.1071659099187672E-4
12	0.0	0.0	0.0	0.0013285990919025207	1.1071659099187672E-4
13	0.0	0.0	0.0	0.0014393156828943973	2.2143318198375344E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACGGA	45	3.427096E-5	16.950722	1
CAACACT	40	0.0052211382	14.272806	4
ACTGGTC	40	0.005298516	14.241196	8
CGGAACC	80	1.2987584E-7	14.2388315	14
CATACTC	95	4.782123E-9	14.022407	3
CGACCAT	75	9.739488E-7	13.923184	10
AAACGCT	55	1.9551851E-4	13.821123	19
TATACGC	85	2.7041642E-7	13.407933	7
CGTTATT	50	0.0014807424	13.321286	2
CTTAGGG	50	0.0014807424	13.321286	3
CGCCAGT	50	0.0015075798	13.289576	18
CTAAGAC	65	5.366405E-5	13.174898	3
CCGTCGT	65	5.4820768E-5	13.144992	9
CGTCGTA	65	5.4849254E-5	13.144264	10
CGACGGG	65	5.4877753E-5	13.143537	14
CCTTAGG	60	3.9649048E-4	12.713041	1
TAGACAG	105	1.9879735E-8	12.667257	5
ATATACG	90	5.411639E-7	12.66375	6
TACGCTA	90	5.4407974E-7	12.65814	9
CGGTCCA	75	1.4880656E-5	12.65744	10
>>END_MODULE