FastQCFastQC Report
Thu 2 Feb 2017
SRR4050273_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050273_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4810172
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA101460.2109280083955418No Hit
GAATAGGACCGCGGTTCTATTTTGT60410.12558802471096667No Hit
GTATCTGATCGTCTTCGAACCTCCG60080.12490197855710772No Hit
GATTAAGAGGGACGGCCGGGGGCAT56790.11806230629590792No Hit
GTCCTATTCCATTATTCCTAGCTGC56460.11737626014204897No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG55090.11452812913966486No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC53360.11093158415125282No Hit
CTATTGGAGCTGGAATTACCGCGGC51830.10775082471063405No Hit
GACTTAATCAACGCAAGCTTATGAC51340.10673215011854045No Hit
GTATCAACGCAGAGTACGGGCGGTG50990.10600452540990218No Hit
GTCTTGCGCCGGTCCAAGAATTTCA49660.10323955151707673No Hit
GGGTAGGCACACGCTGAGCCAGTCA49610.1031356051301284No Hit
GAACTACGACGGTATCTGATCGTCT49610.1031356051301284No Hit
CTTATGACCCGCACTTACTGGGAAT48410.1006408918433686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.015.4982751
CTCGACG1400.014.26337313
TAGATCG1550.013.4983625
GTATCAA79900.013.2909151
AACCGCG4950.012.4881537
CGCTTAC1600.012.47953818
ACGCTTA1650.012.1013717
TAACACG550.003157714112.0455344
CGACCAT7850.011.26769810
CCGTCCA3500.011.1413479
CGTCGTA7900.011.07599310
CGGTCCA11000.010.98077410
CGGTTCT11550.010.86699112
CGAACGA5200.010.78873716
ACGGTAT10050.010.788499
CCGACCA8650.010.7753629
ATACCGT8600.010.61615856
CGTCTTC12200.010.44640510
CCGTCGT8800.010.3755349
GAACCGC6600.010.3748826
Produced by FastQC (version 0.11.5)