##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050273_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4810172
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.87238481285077	31.0	30.0	34.0	26.0	34.0
2	30.014362480177425	31.0	31.0	34.0	26.0	34.0
3	30.117059223661858	31.0	31.0	34.0	26.0	34.0
4	33.660048954590394	37.0	35.0	37.0	28.0	37.0
5	33.68489962521091	37.0	35.0	37.0	28.0	37.0
6	33.70057182986388	37.0	35.0	37.0	29.0	37.0
7	33.57027253911087	37.0	35.0	37.0	28.0	37.0
8	33.576706404677424	37.0	35.0	37.0	29.0	37.0
9	35.13219360970876	39.0	35.0	39.0	29.0	39.0
10	34.99885617395802	39.0	35.0	39.0	28.0	39.0
11	34.87398849770861	39.0	35.0	39.0	27.0	39.0
12	34.805352490513854	39.0	35.0	39.0	27.0	39.0
13	34.79350426554394	39.0	35.0	39.0	27.0	39.0
14	35.90651623268357	40.0	36.0	41.0	27.0	41.0
15	35.855200604053245	40.0	36.0	41.0	27.0	41.0
16	35.86218289075734	40.0	36.0	41.0	27.0	41.0
17	35.8200779514745	40.0	36.0	41.0	27.0	41.0
18	35.772303776247504	40.0	36.0	41.0	27.0	41.0
19	35.7756009556415	40.0	36.0	41.0	27.0	41.0
20	35.73537266442863	40.0	36.0	41.0	27.0	41.0
21	35.66837817857657	40.0	36.0	41.0	27.0	41.0
22	35.5847312736426	40.0	36.0	41.0	26.0	41.0
23	35.49763355655473	40.0	36.0	41.0	26.0	41.0
24	35.42288155184472	40.0	36.0	41.0	25.0	41.0
25	35.17564299156039	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7389205622414536
1101	2	0.7214941371051147
1101	3	0.7587146026017138
1101	4	0.7777751597797291
1101	5	0.8263665765035384
1101	6	0.8302590896846027
1101	7	0.7448492468555443
1101	8	0.7943684757670866
1101	9	0.8650107462483945
1101	10	0.9229266385189732
1101	11	0.8035641299144558
1101	12	0.90104584825702
1101	13	0.8443261816460392
1101	14	1.0304943792066794
1101	15	1.0305695496818785
1101	16	1.0692258822323524
1101	17	1.0461674082581922
1101	18	1.0355246048239692
1101	19	1.1223531570349152
1101	20	1.0443785365293365
1101	21	1.078422285943148
1101	22	1.0972318454694232
1101	23	1.0795557277554622
1101	24	1.1656264311061264
1101	25	1.1928130557628762
1102	1	0.41224622704026004
1102	2	0.46718089440831534
1102	3	0.41895648481117576
1102	4	0.4931364047308904
1102	5	0.3921964027912139
1102	6	0.34180577766301923
1102	7	0.39990893977539343
1102	8	0.4812940863626025
1102	9	0.6009031065093708
1102	10	0.5602455309854832
1102	11	0.48187780685357495
1102	12	0.40007557845703445
1102	13	0.5000979087755582
1102	14	0.6379527219716934
1102	15	0.5385047120684803
1102	16	0.5566360887746242
1102	17	0.5223852500738744
1102	18	0.6112218178198319
1102	19	0.6424511849280563
1102	20	0.5341977870147758
1102	21	0.6322025808891496
1102	22	0.6397721168246733
1102	23	0.6075933836476821
1102	24	0.5757053184602796
1102	25	0.715090071563445
1103	1	0.5055603239297675
1103	2	0.48238951071334313
1103	3	0.5296839557594168
1103	4	0.49849805663235003
1103	5	0.505041684569079
1103	6	0.49838401074835303
1103	7	0.4760816533078014
1103	8	0.5302488086392572
1103	9	0.5525635449831583
1103	10	0.5414720907930288
1103	11	0.5278384915268148
1103	12	0.482017873630582
1103	13	0.5651397266420233
1103	14	0.7219304986982991
1103	15	0.6396682466186263
1103	16	0.6475233303271466
1103	17	0.5538462962751325
1103	18	0.6278158410071768
1103	19	0.6144961720774802
1103	20	0.6442563748255381
1103	21	0.5676262444180651
1103	22	0.715891445846701
1103	23	0.7505108133524416
1103	24	0.7918259764637909
1103	25	0.7742084612883886
1104	1	0.5543995830629704
1104	2	0.6015883410602996
1104	3	0.5977531287699342
1104	4	0.5624124979774834
1104	5	0.6176534795826356
1104	6	0.5631247672482189
1104	7	0.5666433558389627
1104	8	0.5659731977188187
1104	9	0.7355714142828731
1104	10	0.6556233950930945
1104	11	0.5886278408579884
1104	12	0.5771456320005584
1104	13	0.5593742762811971
1104	14	0.616176312771266
1104	15	0.5757407331034088
1104	16	0.6768639535678815
1104	17	0.6147850130251129
1104	18	0.6831906901592859
1104	19	0.7427486077454404
1104	20	0.7887840839209517
1104	21	0.6901683051794976
1104	22	0.7681333560159942
1104	23	0.7290026934080771
1104	24	0.7929185018702753
1104	25	0.8663511813712077
1105	1	0.38604677800444875
1105	2	0.4067709770529433
1105	3	0.4207383029929943
1105	4	0.4675441182295117
1105	5	0.4894922705598148
1105	6	0.39975288510103724
1105	7	0.3715111436321408
1105	8	0.43987039490254887
1105	9	0.4213582029281042
1105	10	0.5136262472389319
1105	11	0.4080970906001298
1105	12	0.3718981679887179
1105	13	0.4735755947259719
1105	14	0.48373894786701044
1105	15	0.4979151803880413
1105	16	0.4214691466258671
1105	17	0.47125136577635374
1105	18	0.5858851787013677
1105	19	0.6133911298316335
1105	20	0.582078778750315
1105	21	0.5605364651866722
1105	22	0.572741813794373
1105	23	0.5513895268983688
1105	24	0.6417846573032548
1105	25	0.6094944792769397
1106	1	0.4586403638563681
1106	2	0.43682665432187306
1106	3	0.4359043053686058
1106	4	0.5262054177763886
1106	5	0.5215159034840156
1106	6	0.5291656870015231
1106	7	0.5029770184286733
1106	8	0.4808471051220735
1106	9	0.4634393960124399
1106	10	0.57715946619102
1106	11	0.5432820274704824
1106	12	0.531283623973934
1106	13	0.47286428418090054
1106	14	0.5795092703341211
1106	15	0.5056846581936796
1106	16	0.45680798785590326
1106	17	0.558376402070401
1106	18	0.5707215871303859
1106	19	0.5341418931064581
1106	20	0.635293789088621
1106	21	0.5416603752654154
1106	22	0.6360863212951386
1106	23	0.37073421037028
1106	24	0.5825025491689857
1106	25	0.6109253182193513
1107	1	0.4015446235115441
1107	2	0.39968539288759075
1107	3	0.26496279064407346
1107	4	0.37042027867793337
1107	5	0.43531094283166283
1107	6	0.3914403259838508
1107	7	0.48948122595829346
1107	8	0.44247021974052103
1107	9	0.5585409669848573
1107	10	0.557423830002989
1107	11	0.5097038868866974
1107	12	0.3893182415567651
1107	13	0.4568050219461526
1107	14	0.5423118678179648
1107	15	0.54338058588813
1107	16	0.5118073494634885
1107	17	0.5312564104426727
1107	18	0.49306149679561173
1107	19	0.4889399825203711
1107	20	0.5298569535620175
1107	21	0.5115780486513728
1107	22	0.47973533279945
1107	23	0.4493114046352744
1107	24	0.47822844780947094
1107	25	0.5397417379912497
1108	1	0.09471460680632049
1108	2	-0.004880573183385195
1108	3	0.007341243021613764
1108	4	0.05911993887909972
1108	5	0.078741878122365
1108	6	0.04818592790056897
1108	7	-0.08263244077443233
1108	8	-0.013972474010337521
1108	9	-0.021700831805866017
1108	10	-0.10822789611998473
1108	11	-0.08776723445082268
1108	12	-0.11078537565639834
1108	13	-0.08392636962943101
1108	14	-0.16745175505133858
1108	15	-0.04225879298692092
1108	16	-0.07053106151775523
1108	17	-0.06387307808991238
1108	18	-0.09158898113557967
1108	19	0.01862046073662782
1108	20	0.00725992440748513
1108	21	0.08308083013181289
1108	22	0.0509953153771292
1108	23	-0.012903261792494902
1108	24	0.009807310267134994
1108	25	-0.07848885188823118
1109	1	-0.13486691601574208
1109	2	-0.04141813331033006
1109	3	-0.06302996001502947
1109	4	-0.03956003225801652
1109	5	0.01237559094983709
1109	6	-0.03498301963621486
1109	7	-0.03321530386117644
1109	8	-0.05449405414395869
1109	9	-0.07147772769576477
1109	10	-0.21226741054000087
1109	11	-0.08836962783976787
1109	12	-0.050978249303540224
1109	13	-0.11377164834935627
1109	14	-0.09198900105006658
1109	15	-0.05496669035144919
1109	16	-0.0823192043966543
1109	17	-0.12440962309239723
1109	18	-0.0514267895443794
1109	19	-0.011309327498594257
1109	20	-0.12264487298840265
1109	21	-0.07612378159907252
1109	22	-0.12170153765958958
1109	23	-0.12504881980971305
1109	24	-0.08279989701549795
1109	25	-0.072942003594747
1110	1	-0.11455897279427418
1110	2	-0.16526693562011374
1110	3	-0.09497032320508936
1110	4	-0.1662471490761277
1110	5	-0.10429388492474345
1110	6	-0.06320174748682206
1110	7	0.021016148956952918
1110	8	-0.03277028667359616
1110	9	0.028753730084652318
1110	10	-0.14620506266522426
1110	11	-0.1468686176000844
1110	12	-0.15222834958316867
1110	13	-0.1738442135807361
1110	14	-0.18732729281456528
1110	15	-0.15815297722644317
1110	16	-0.03295385656902283
1110	17	-0.08471796329712333
1110	18	-0.12040704707072791
1110	19	-0.14004188401288076
1110	20	-0.13930822870442228
1110	21	-0.13739282128830155
1110	22	-0.10866819685525542
1110	23	-0.09555828992804294
1110	24	-0.09228392629631799
1110	25	-0.02758368898717123
1111	1	-0.025154231969040097
1111	2	-0.029970225657152127
1111	3	-0.05971184219069414
1111	4	0.07295547201481156
1111	5	-0.07066799539987301
1111	6	-0.03278613774961059
1111	7	-0.08316510094977048
1111	8	-0.17233863594372423
1111	9	-0.08942684946427448
1111	10	-0.10177110158066682
1111	11	-0.1891337977557157
1111	12	-0.14014793730101616
1111	13	-0.17046945009160197
1111	14	-0.18561216099971745
1111	15	-0.1488220160938809
1111	16	-0.15971817820685885
1111	17	-0.18598292282356965
1111	18	-0.0826559482607152
1111	19	-0.1431552321596996
1111	20	-0.13576075329530113
1111	21	-0.1479618388995121
1111	22	-0.08769112817423519
1111	23	-0.030151019328954476
1111	24	-0.16250675371387047
1111	25	-0.06307038206211502
1112	1	-0.13659611331906163
1112	2	-0.16567687586230662
1112	3	-0.11815070342937162
1112	4	-0.24348346016111577
1112	5	-0.12283600043880227
1112	6	-0.1910207706098319
1112	7	-0.25365208518035587
1112	8	-0.17978738119617788
1112	9	-0.20817666673566748
1112	10	-0.24633476522801345
1112	11	-0.2504223905735259
1112	12	-0.2673640408011053
1112	13	-0.2736573482283191
1112	14	-0.31613673529632536
1112	15	-0.28411630179304126
1112	16	-0.258213613731769
1112	17	-0.15447446220500893
1112	18	-0.24911791914524883
1112	19	-0.4495125850657331
1112	20	-0.3041705097523888
1112	21	-0.2930029379111758
1112	22	-0.2506653137714281
1112	23	-0.3140727574486917
1112	24	-0.3629247747581985
1112	25	-0.38676186123432643
1113	1	-0.4421882434115254
1113	2	-0.45170108140006704
1113	3	-0.3082509638248432
1113	4	-0.2700295779156079
1113	5	-0.335019999533408
1113	6	-0.363664197287477
1113	7	-0.4907440339944671
1113	8	-0.4488475693201792
1113	9	-0.3355648507378106
1113	10	-0.5437316766713778
1113	11	-0.4685037660388218
1113	12	-0.652397825964421
1113	13	-0.53335088549575
1113	14	-0.5318355545265447
1113	15	-0.4826864824993322
1113	16	-0.45765587227058546
1113	17	-0.550794288040727
1113	18	-0.43485969406255265
1113	19	-0.45291413597839636
1113	20	-0.48276133210884353
1113	21	-0.530193592762437
1113	22	-0.5710183272991003
1113	23	-0.5714938190325967
1113	24	-0.6225334077005229
1113	25	-0.5375058734567446
1114	1	-0.6122891975276019
1114	2	-0.6275626706255757
1114	3	-0.681741523692363
1114	4	-0.6427430403448895
1114	5	-0.6477822207532142
1114	6	-0.6619307137265409
1114	7	-0.7011850544902885
1114	8	-0.699653941361845
1114	9	-0.7966179408211005
1114	10	-0.8565148002951588
1114	11	-0.8770829442327113
1114	12	-0.92160258389098
1114	13	-0.8820871553696037
1114	14	-1.1933050519630726
1114	15	-1.2512591112733134
1114	16	-1.1539371997485475
1114	17	-1.089343715146292
1114	18	-1.1235205219910398
1114	19	-0.866578217264923
1114	20	-1.0666050324726584
1114	21	-1.0792917664092698
1114	22	-1.1843320573240987
1114	23	-1.1148198949613644
1114	24	-1.1057433831368257
1114	25	-1.0833321466098127
1115	1	-0.4606285021537566
1115	2	-0.49192653997628
1115	3	-0.5599282757809014
1115	4	-0.3466439723000363
1115	5	-0.4224055101477262
1115	6	-0.5551997846418786
1115	7	-0.5311197506768011
1115	8	-0.6097904268948611
1115	9	-0.5597405445999115
1115	10	-0.6830413862482132
1115	11	-0.7079159175299523
1115	12	-0.7504119177067352
1115	13	-0.6974867651949239
1115	14	-0.8175796194808314
1115	15	-0.7011689769400178
1115	16	-0.7775483879819163
1115	17	-0.7816461951992508
1115	18	-0.7141534062250159
1115	19	-0.7831332384390777
1115	20	-0.719762926369441
1115	21	-0.8705776635762419
1115	22	-1.0245414206595953
1115	23	-0.9012573638948638
1115	24	-0.8285940726462044
1115	25	-0.88918879912341
1116	1	-0.3517789759950283
1116	2	-0.6162820520579935
1116	3	-0.4448837269047239
1116	4	-0.5138642216603344
1116	5	-0.48701662007748325
1116	6	-0.44260347472062733
1116	7	-0.39222649077646565
1116	8	-0.5124619418401721
1116	9	-0.5974734781629039
1116	10	-0.5145424388350293
1116	11	-0.5847582988569897
1116	12	-0.6672866208914314
1116	13	-0.7293308831130005
1116	14	-0.5914853899247561
1116	15	-0.7659232670286897
1116	16	-0.6530638719946467
1116	17	-0.7251027910555337
1116	18	-0.6337116741703852
1116	19	-0.6997300870579508
1116	20	-0.6495238438565565
1116	21	-0.7458011167697975
1116	22	-0.7528988890642694
1116	23	-0.704825526865072
1116	24	-0.7330007610025433
1116	25	-0.7683022162384034
1201	1	0.5545527553873235
1201	2	0.6829116340265564
1201	3	0.6430618355724249
1201	4	0.7307159006743191
1201	5	0.6970164493427973
1201	6	0.825828706318056
1201	7	0.7750047354468919
1201	8	0.8443427697006101
1201	9	0.9234245464929955
1201	10	0.8893716430386078
1201	11	0.7630101762221031
1201	12	0.8206690888287795
1201	13	0.8677685941874671
1201	14	0.9343821358045403
1201	15	0.9550104954122745
1201	16	0.8735145962758395
1201	17	0.9112580432130812
1201	18	0.9706567457449893
1201	19	0.958637871888719
1201	20	0.8781362908666352
1201	21	0.9102657058974941
1201	22	0.9770487604825817
1201	23	0.9892613631934708
1201	24	0.9488362458869943
1201	25	0.8416144577368243
1202	1	0.3703238633093022
1202	2	0.42720681140020034
1202	3	0.4880575945976986
1202	4	0.6159930389631469
1202	5	0.5738328230360423
1202	6	0.5243018644169766
1202	7	0.5236664678626823
1202	8	0.4573142955830036
1202	9	0.528255755990152
1202	10	0.6493546776837036
1202	11	0.521525813956444
1202	12	0.4801636269585714
1202	13	0.4549564788681906
1202	14	0.5776413046783304
1202	15	0.554104085800148
1202	16	0.5866369714197006
1202	17	0.5559573817287671
1202	18	0.6283771979884705
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2210	12	-0.07729782299098531
2210	13	-0.010968830230183357
2210	14	-0.08729240601255128
2210	15	-0.1318882105749566
2210	16	-0.04924014928994325
2210	17	-0.10141934331910818
2210	18	-0.10023515872450162
2210	19	-0.07236976551622831
2210	20	-0.10289213914828821
2210	21	-0.09256878830034054
2210	22	-0.08882404271611222
2210	23	-0.08857606234408877
2210	24	-0.013901703675614385
2210	25	-0.057360511118112356
2211	1	-0.14159381605495724
2211	2	-0.17207291733649654
2211	3	-0.14454486532383726
2211	4	-0.22592947913022243
2211	5	-0.21182093076031805
2211	6	-0.22185206539401037
2211	7	-0.13789347682990893
2211	8	-0.18741244338128382
2211	9	-0.12617050994318646
2211	10	-0.1822093963254261
2211	11	-0.11795274438336634
2211	12	-0.1180097528033599
2211	13	-0.07117490032353402
2211	14	-0.22685478150592786
2211	15	-0.11506666779677488
2211	16	-0.17166253654244912
2211	17	-0.2428753668969108
2211	18	-0.21103495331183098
2211	19	-0.3067233916205083
2211	20	-0.3347852476523272
2211	21	-0.2937084193017796
2211	22	-0.3108241928062867
2211	23	-0.16016327838215716
2211	24	-0.38328398956143417
2211	25	-0.2558117913501192
2212	1	-0.3149424376818253
2212	2	-0.23116823863333025
2212	3	-0.3561215009285732
2212	4	-0.3096862739273476
2212	5	-0.26228321963626655
2212	6	-0.3555578992126911
2212	7	-0.3701202289168819
2212	8	-0.39738744823470995
2212	9	-0.38916434609150485
2212	10	-0.37185218413361554
2212	11	-0.3050195760665346
2212	12	-0.32666947907010524
2212	13	-0.28472930723481227
2212	14	-0.3132130129173092
2212	15	-0.38261423137666384
2212	16	-0.32296810827609335
2212	17	-0.3537349087334505
2212	18	-0.5258912918868646
2212	19	-0.467513772129152
2212	20	-0.4543251428183126
2212	21	-0.3734174563819579
2212	22	-0.35222877444092404
2212	23	-0.35956145349378943
2212	24	-0.47855553877517565
2212	25	-0.4021741481740406
2213	1	-0.47946978461031264
2213	2	-0.49497410802478825
2213	3	-0.5353124253703321
2213	4	-0.5361809399171378
2213	5	-0.5018223579136887
2213	6	-0.37333101745741715
2213	7	-0.374508050883712
2213	8	-0.4587954883144931
2213	9	-0.4186353825780813
2213	10	-0.4799021650514348
2213	11	-0.30797204882942
2213	12	-0.39880458632637783
2213	13	-0.3524926143571463
2213	14	-0.5294420045139461
2213	15	-0.5204968651499655
2213	16	-0.3595575641098421
2213	17	-0.544363666663358
2213	18	-0.45271886096076486
2213	19	-0.42685193541502997
2213	20	-0.5653163002067174
2213	21	-0.5046229912951503
2213	22	-0.5642742224401758
2213	23	-0.489120205014963
2213	24	-0.574728795995803
2213	25	-0.49435720391561944
2214	1	-0.5857786195977042
2214	2	-0.6466240478226766
2214	3	-0.7063287877826347
2214	4	-0.8567907647327218
2214	5	-0.74000571492261
2214	6	-0.747405225982888
2214	7	-0.7908284045286962
2214	8	-0.797626579243115
2214	9	-0.7769179898783563
2214	10	-0.8231373446377503
2214	11	-0.7188882450168634
2214	12	-0.6876487857670952
2214	13	-0.809942291253968
2214	14	-0.9040327510156274
2214	15	-0.7684458015873261
2214	16	-0.9068463904428867
2214	17	-0.8556440587404026
2214	18	-0.937869131713768
2214	19	-0.8952612150015753
2214	20	-0.9313473365746958
2214	21	-0.9992290025565822
2214	22	-0.8915511476280216
2214	23	-0.8748690164504609
2214	24	-0.9861942677436417
2214	25	-0.8191005455986797
2215	1	-0.39158756542989437
2215	2	-0.38906336638140004
2215	3	-0.5246041212494283
2215	4	-0.4215215383361439
2215	5	-0.4315754062078625
2215	6	-0.3770188320706822
2215	7	-0.37274946242846596
2215	8	-0.4394588980267429
2215	9	-0.551163009193111
2215	10	-0.48814749013480707
2215	11	-0.4398049296018911
2215	12	-0.4278169835264336
2215	13	-0.37847188002150745
2215	14	-0.5062277187996571
2215	15	-0.4411555266826639
2215	16	-0.5473813584246372
2215	17	-0.5175940887691013
2215	18	-0.5821895687733729
2215	19	-0.6930745267340228
2215	20	-0.6123242515527139
2215	21	-0.5925908075406028
2215	22	-0.6748743478217918
2215	23	-0.42655491754607056
2215	24	-0.5531284740098741
2215	25	-0.5177267328442738
2216	1	0.15746628904171445
2216	2	-0.03959215294151619
2216	3	-0.04307769510812065
2216	4	0.18300182849140612
2216	5	0.10287582938566686
2216	6	0.19402186207706507
2216	7	0.2215598183690659
2216	8	0.07501482088561318
2216	9	0.21059029407455654
2216	10	0.2560503630708766
2216	11	0.41058836364388185
2216	12	0.2587503873098669
2216	13	0.2935133113174757
2216	14	0.31533549822700735
2216	15	0.3692303160791539
2216	16	0.08329815208515612
2216	17	0.042004178498878275
2216	18	0.176146401899409
2216	19	0.17469553682585826
2216	20	0.3651136391031926
2216	21	0.3531090104829744
2216	22	0.3644813501500934
2216	23	0.22311640402472221
2216	24	0.15895449668928308
2216	25	0.12664485509781542
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	179120.0
3	5.0
4	1495.0
5	8344.0
6	10295.0
7	9806.0
8	8582.0
9	8381.0
10	8219.0
11	9149.0
12	14169.0
13	11377.0
14	6483.0
15	6724.0
16	7001.0
17	7231.0
18	8148.0
19	9706.0
20	12304.0
21	15288.0
22	18808.0
23	22613.0
24	26000.0
25	30471.0
26	33991.0
27	38677.0
28	44745.0
29	54142.0
30	67859.0
31	86684.0
32	111530.0
33	148029.0
34	202831.0
35	286304.0
36	432709.0
37	811969.0
38	2050983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84875674505363	17.243334055026313	12.345405036306708	25.562504163613347
2	16.979927161183003	20.135449966362383	37.530418045503545	25.354204826951072
3	18.932879695064457	23.79672574126459	28.066556380756275	29.203838182914684
4	13.018813731209859	16.091387823801984	36.6501281679841	34.23967027700406
5	15.735796188796982	35.09385762731067	32.959768876126425	16.210577307765924
6	33.36460769057941	36.28485599926773	16.04474382764847	14.30579248250439
7	29.60683606368467	30.264404286413775	21.012396720000698	19.116362929900852
8	27.085566013559166	32.403560695065245	19.572236496583585	20.938636794792004
9	27.26307080597626	14.859579446688995	18.553499482936488	39.32385026439826
10	16.52448732118885	25.842523658938493	30.450178772142127	27.182810247730526
11	36.93849814324973	21.915095407931414	21.820839768972167	19.325566679846688
12	25.15862336781393	23.605751597009082	27.950959565452006	23.284665469724985
13	29.58837638654323	19.934294547225743	24.514783099195064	25.962545967035965
14	24.022460216848177	20.01243651758259	24.59007509823782	31.375028167331422
15	25.24484224392832	26.491231961139928	22.445497388377294	25.818428406554457
16	25.6433171371077	26.139498737941498	23.132884726968296	25.084299397982505
17	25.153965977363573	25.27159420562762	24.105585039329718	25.46885477767909
18	26.615633109134713	24.01459986896695	24.867758525047464	24.502008496850873
19	27.161881016064882	24.549656290625407	24.098428538936467	24.190034154373247
20	26.181240107506454	24.497120243337555	24.110920643264233	25.210719005891765
21	26.739532196852007	24.500987448658215	23.957770380892182	24.801709973597596
22	25.787244566600158	24.255606645955247	24.740319141101008	25.216829646343587
23	24.812219458884094	23.66053646323217	24.9460465668964	26.581197510987337
24	25.044920340147613	25.283435420577405	24.43580722290615	25.23583701636883
25	25.12166730336886	24.50062115075867	24.58876221328003	25.78894933259244
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1993.0
1	1993.0
2	2140.0
3	2287.0
4	2287.0
5	2287.0
6	4049.5
7	5812.0
8	5812.0
9	5812.0
10	8432.0
11	11052.0
12	11052.0
13	11052.0
14	15641.5
15	20231.0
16	20231.0
17	20231.0
18	33069.5
19	45908.0
20	45908.0
21	45908.0
22	65422.5
23	84937.0
24	84937.0
25	84937.0
26	116273.0
27	147609.0
28	147609.0
29	147609.0
30	180896.0
31	214183.0
32	214183.0
33	214183.0
34	263344.5
35	312506.0
36	312506.0
37	312506.0
38	351488.0
39	390470.0
40	390470.0
41	390470.0
42	443491.0
43	496512.0
44	496512.0
45	496512.0
46	531067.0
47	565622.0
48	565622.0
49	565622.0
50	577056.0
51	588490.0
52	588490.0
53	588490.0
54	551995.0
55	515500.0
56	515500.0
57	515500.0
58	483333.0
59	451166.0
60	451166.0
61	451166.0
62	401347.5
63	351529.0
64	351529.0
65	351529.0
66	297544.5
67	243560.0
68	243560.0
69	243560.0
70	192822.0
71	142084.0
72	142084.0
73	142084.0
74	117906.5
75	93729.0
76	93729.0
77	93729.0
78	79501.5
79	65274.0
80	65274.0
81	65274.0
82	49957.5
83	34641.0
84	34641.0
85	34641.0
86	25883.0
87	17125.0
88	17125.0
89	17125.0
90	12142.0
91	7159.0
92	7159.0
93	7159.0
94	3906.5
95	654.0
96	654.0
97	654.0
98	396.5
99	139.0
100	139.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13829027319605203
2	0.15618984102855366
3	0.16855946107540437
4	0.18816374965385851
5	0.1786006820546126
6	0.0676067300711908
7	0.054260013987025825
8	0.05812681958150353
9	0.06692068391733184
10	0.06683752680777319
11	0.6806201524602447
12	0.6396652760026045
13	0.637399244767131
14	0.638210026585328
15	0.6396444867252148
16	0.646026794883842
17	0.6383763408044452
18	0.6491451864922918
19	0.6442389170283308
20	0.650621225186958
21	0.6378773981470933
22	0.6412244718068293
23	0.6262561920862706
24	0.6361518881237511
25	0.6306219403381002
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4810172.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.099685359730984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.90279739248346	42.5360343552441
2	9.399237570027882	9.417976897595185
3	2.3156012542323103	3.480326827669115
4	0.9565866746917092	1.9169876568546387
5	0.49549755211017993	1.241213572861846
6	0.3234091612873938	0.972161833377175
7	0.2181514285211935	0.7650522550781347
8	0.17453027593613266	0.6995129528117818
9	0.1341473046952927	0.6048663981381115
>10	0.858824545462267	8.438438291701418
>50	0.1044696129240493	3.67024687166294
>100	0.09084688223485819	9.2897255856422
>500	0.01359663888494512	4.647996037664866
>1k	0.011886631711382187	11.070317572181992
>5k	3.7536731720154117E-4	1.0371390144097137
>10k+	4.1707479689060126E-5	0.21200387710689944
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	10146	0.2109280083955418	No Hit
GAATAGGACCGCGGTTCTATTTTGT	6041	0.12558802471096667	No Hit
GTATCTGATCGTCTTCGAACCTCCG	6008	0.12490197855710772	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	5679	0.11806230629590792	No Hit
GTCCTATTCCATTATTCCTAGCTGC	5646	0.11737626014204897	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	5509	0.11452812913966486	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	5336	0.11093158415125282	No Hit
CTATTGGAGCTGGAATTACCGCGGC	5183	0.10775082471063405	No Hit
GACTTAATCAACGCAAGCTTATGAC	5134	0.10673215011854045	No Hit
GTATCAACGCAGAGTACGGGCGGTG	5099	0.10600452540990218	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	4966	0.10323955151707673	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	4961	0.1031356051301284	No Hit
GAACTACGACGGTATCTGATCGTCT	4961	0.1031356051301284	No Hit
CTTATGACCCGCACTTACTGGGAAT	4841	0.1006408918433686	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0789277389665068E-5
2	0.0	0.0	0.0	0.0	2.0789277389665068E-5
3	0.0	0.0	0.0	0.0	2.0789277389665068E-5
4	0.0	0.0	0.0	4.1578554779330135E-5	2.0789277389665068E-5
5	0.0	0.0	0.0	4.1578554779330135E-5	2.0789277389665068E-5
6	2.0789277389665068E-5	0.0	0.0	4.1578554779330135E-5	2.0789277389665068E-5
7	2.0789277389665068E-5	0.0	0.0	4.1578554779330135E-5	2.0789277389665068E-5
8	2.0789277389665068E-5	0.0	0.0	4.1578554779330135E-5	2.0789277389665068E-5
9	2.0789277389665068E-5	0.0	0.0	1.0394638694832533E-4	6.23678321689952E-5
10	2.0789277389665068E-5	0.0	0.0	1.0394638694832533E-4	6.23678321689952E-5
11	2.0789277389665068E-5	0.0	0.0	1.0394638694832533E-4	6.23678321689952E-5
12	2.0789277389665068E-5	0.0	0.0	1.4552494172765548E-4	2.0789277389665067E-4
13	2.0789277389665068E-5	0.0	0.0	1.4552494172765548E-4	2.702606060656459E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2455	0.0	15.498275	1
CTCGACG	140	0.0	14.263373	13
TAGATCG	155	0.0	13.498362	5
GTATCAA	7990	0.0	13.290915	1
AACCGCG	495	0.0	12.488153	7
CGCTTAC	160	0.0	12.479538	18
ACGCTTA	165	0.0	12.10137	17
TAACACG	55	0.0031577141	12.045534	4
CGACCAT	785	0.0	11.267698	10
CCGTCCA	350	0.0	11.141347	9
CGTCGTA	790	0.0	11.075993	10
CGGTCCA	1100	0.0	10.980774	10
CGGTTCT	1155	0.0	10.866991	12
CGAACGA	520	0.0	10.788737	16
ACGGTAT	1005	0.0	10.78849	9
CCGACCA	865	0.0	10.775362	9
ATACCGT	860	0.0	10.6161585	6
CGTCTTC	1220	0.0	10.446405	10
CCGTCGT	880	0.0	10.375534	9
GAACCGC	660	0.0	10.374882	6
>>END_MODULE