FastQCFastQC Report
Thu 2 Feb 2017
SRR4050273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4810172
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA98670.20512780000382522No Hit
GTATCAACGCAGAGTACTTTTTTTT72800.1513459393967617No Hit
GAATAGGACCGCGGTTCTATTTTGT65290.13573319207712323No Hit
GATTAAGAGGGACGGCCGGGGGCAT58920.12249042237990658No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG55220.1147983897457305No Hit
GTATCTGATCGTCTTCGAACCTCCG53060.11030790582956286No Hit
CTTATGACCCGCACTTACTGGGAAT51530.10712714638894409No Hit
ATACAGGACTCTTTCGAGGCCCTGT51380.10681530722809912No Hit
ATCAGATACCGTCGTAGTTCCGACC51010.1060461039646815No Hit
CTATTGGAGCTGGAATTACCGCGGC50850.10571347552644687No Hit
GACTTAATCAACGCAAGCTTATGAC49780.1034890228457527No Hit
GAACTACGACGGTATCTGATCGTCT49770.10346823356836304No Hit
GTCCTATTCCATTATTCCTAGCTGC48750.1013477272746172No Hit
TCGTAGTTCCGACCATAAACGATGC48720.10128535944244821No Hit
GGGTAGGCACACGCTGAGCCAGTCA48580.10099430955899291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.015.8372811
CTCGACG1750.014.10326613
CGCTTAC1750.014.10267918
GTATCAA81400.013.29398251
ACGCTTA1900.012.98930917
TCGACGC2100.011.75223314
CGTCGTA8100.011.72313910
AACCGCG4950.011.5115467
ATACCGT8500.011.5115136
CGACCAT9100.011.4783710
CGGTTCT12200.011.44075812
GCTCGTA4650.011.4359699
GGTAGCG2650.011.10822711
TACCGTC8550.011.1076327
CGGTAGC2750.011.0495910
CGAGCCG11350.011.03937215
CCGGTAG2700.010.9027459
AGGCCCG12000.010.76184110
GGTTCTA13350.010.66582313
ACCGTCG9250.010.5738568
Produced by FastQC (version 0.11.5)