##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050273_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4810172
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.776341677594896	31.0	31.0	34.0	27.0	34.0
2	30.8773295424779	31.0	31.0	34.0	27.0	34.0
3	30.985292209925134	31.0	31.0	34.0	27.0	34.0
4	34.531309275427155	37.0	35.0	37.0	31.0	37.0
5	34.409278503970334	37.0	35.0	37.0	30.0	37.0
6	34.449707827495565	37.0	35.0	37.0	31.0	37.0
7	34.386701140832386	37.0	35.0	37.0	31.0	37.0
8	34.344313675269824	37.0	35.0	37.0	30.0	37.0
9	35.959914323230024	39.0	37.0	39.0	31.0	39.0
10	35.815767918486074	39.0	35.0	39.0	30.0	39.0
11	35.82061161222509	39.0	35.0	39.0	31.0	39.0
12	35.70017433888019	39.0	35.0	39.0	30.0	39.0
13	35.7221793732116	39.0	35.0	39.0	30.0	39.0
14	36.861420963741004	40.0	37.0	41.0	31.0	41.0
15	36.827803454845274	40.0	37.0	41.0	31.0	41.0
16	36.788959937399326	40.0	37.0	41.0	31.0	41.0
17	36.75753091573441	40.0	37.0	41.0	31.0	41.0
18	36.70530180625558	40.0	37.0	41.0	31.0	41.0
19	36.713346632927056	40.0	37.0	41.0	30.0	41.0
20	36.6578301150146	40.0	37.0	41.0	30.0	41.0
21	36.60765103617916	40.0	37.0	41.0	30.0	41.0
22	36.55144369057905	40.0	37.0	41.0	30.0	41.0
23	36.46077790981279	40.0	37.0	41.0	30.0	41.0
24	36.378843417657414	40.0	37.0	41.0	30.0	41.0
25	36.06309878316202	39.0	36.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.608329885846647
1101	2	0.5582069899795243
1101	3	0.5379799723802243
1101	4	0.5569210091481551
1101	5	0.5768132421478853
1101	6	0.6080779257382574
1101	7	0.6182230659272818
1101	8	0.6524629595696112
1101	9	0.7488260290734203
1101	10	0.8320151194040903
1101	11	0.777685013748858
1101	12	0.7560719821341166
1101	13	0.7323694916141292
1101	14	0.7794684217267331
1101	15	0.8517007942318955
1101	16	0.9014941821014801
1101	17	0.8692644796288036
1101	18	0.9657973373993229
1101	19	0.9017627785797586
1101	20	0.9564238710765522
1101	21	0.9307278311635159
1101	22	0.858894164890458
1101	23	0.9502799476963801
1101	24	1.007756152046035
1101	25	1.0954327186412982
1102	1	0.4361397185093665
1102	2	0.4075942001574262
1102	3	0.4273963168298529
1102	4	0.3652292041629934
1102	5	0.3359825304370432
1102	6	0.4210788635168754
1102	7	0.4902011166720399
1102	8	0.5121864457654794
1102	9	0.5177160317989831
1102	10	0.6355525004301583
1102	11	0.5575388650316455
1102	12	0.6217622965286296
1102	13	0.6444545168756406
1102	14	0.6211188043065263
1102	15	0.6234068275823219
1102	16	0.40571110615680794
1102	17	0.5251786443975845
1102	18	0.5855733792344111
1102	19	0.6618095597200906
1102	20	0.7398626082629391
1102	21	0.660723735118971
1102	22	0.7675335851615657
1102	23	0.6826492476885306
1102	24	0.7229542481552542
1102	25	0.9414700265237244
1103	1	0.39489624234431275
1103	2	0.37579550255927785
1103	3	0.3991185191548965
1103	4	0.3451542631224669
1103	5	0.4403313407733691
1103	6	0.38992269467766505
1103	7	0.4548585435386343
1103	8	0.442601538490095
1103	9	0.5029139937270415
1103	10	0.5614975977273744
1103	11	0.5726963493372708
1103	12	0.518860949752316
1103	13	0.5170574509964538
1103	14	0.5825371203743686
1103	15	0.6728407715713871
1103	16	0.5700109469042829
1103	17	0.6269149924916206
1103	18	0.6515774697943613
1103	19	0.6328058903796929
1103	20	0.5908050588597433
1103	21	0.5891290249157706
1103	22	0.6853814685896182
1103	23	0.6764998926950483
1103	24	0.6788302609405079
1103	25	0.7550339719318657
1104	1	0.38578816659231663
1104	2	0.3719568313987196
1104	3	0.35630322033773965
1104	4	0.36424845010627394
1104	5	0.4072311577519869
1104	6	0.38255704935999546
1104	7	0.42657550102534003
1104	8	0.40602571662186193
1104	9	0.4576377901404385
1104	10	0.44630501166088266
1104	11	0.374010511278982
1104	12	0.46035011816685767
1104	13	0.5023728388253659
1104	14	0.5185435859833589
1104	15	0.4963626804000896
1104	16	0.49638846670256953
1104	17	0.5979995172900061
1104	18	0.624670470272477
1104	19	0.6757918974374917
1104	20	0.7232601330379822
1104	21	0.6808356698720033
1104	22	0.6399666779993609
1104	23	0.6705972303835281
1104	24	0.6584597131373755
1104	25	0.7918294355918363
1105	1	0.4054752556994501
1105	2	0.3277936491932394
1105	3	0.3223594022293028
1105	4	0.2602815457607903
1105	5	0.33664368746183726
1105	6	0.43469980015599674
1105	7	0.39432673650674843
1105	8	0.4016883741401358
1105	9	0.4825909629284624
1105	10	0.49573101282685883
1105	11	0.5053647603484492
1105	12	0.560176621597499
1105	13	0.5630638832668211
1105	14	0.5632675646371084
1105	15	0.5807181223481592
1105	16	0.5731909959639765
1105	17	0.5726827766289873
1105	18	0.5081838475539726
1105	19	0.5653566671581061
1105	20	0.6194075118442015
1105	21	0.7165342034385276
1105	22	0.6265870286877373
1105	23	0.570403517660985
1105	24	0.6839360663370755
1105	25	0.6713499163308896
1106	1	0.32917744214087463
1106	2	0.3151308186974404
1106	3	0.3165475505729738
1106	4	0.2941516790589276
1106	5	0.2908942703662447
1106	6	0.34603089865584025
1106	7	0.3588082287420207
1106	8	0.3957110568201756
1106	9	0.48107562264426207
1106	10	0.46125221295003627
1106	11	0.48471984371477816
1106	12	0.5608262718548573
1106	13	0.5032441481033914
1106	14	0.5464755185326666
1106	15	0.5087152188243422
1106	16	0.513387538131056
1106	17	0.537722678085153
1106	18	0.6080302599423391
1106	19	0.6391215169227493
1106	20	0.578472330813895
1106	21	0.5376458835229982
1106	22	0.6312442457041456
1106	23	0.6629991975694765
1106	24	0.7127431227798198
1106	25	0.7372970018088694
1107	1	0.3442353888225895
1107	2	0.27828879720514976
1107	3	0.33371011163550435
1107	4	0.3257182246887993
1107	5	0.3914221075003752
1107	6	0.3744897458557688
1107	7	0.3855131439336148
1107	8	0.4366374720557076
1107	9	0.5097405863301034
1107	10	0.5162974167504828
1107	11	0.4583119665141524
1107	12	0.4962794066737004
1107	13	0.39536137844986996
1107	14	0.5540628002300281
1107	15	0.42949689271074476
1107	16	0.4775991935467374
1107	17	0.46398738339024703
1107	18	0.5072472912393806
1107	19	0.41193456171057363
1107	20	0.42261711072316643
1107	21	0.2596003095867161
1107	22	0.17412684301599057
1107	23	0.4713202456343595
1107	24	0.5564977759184373
1107	25	0.7273010110935445
1108	1	0.16051911329452295
1108	2	0.08954193417654466
1108	3	0.1864041010715063
1108	4	0.1827810904466176
1108	5	0.21042062688336927
1108	6	0.2258434279258239
1108	7	0.10907513423451576
1108	8	0.11134786852459655
1108	9	0.21239100629171048
1108	10	0.13028694708816602
1108	11	0.18364811319524676
1108	12	0.17211292965006209
1108	13	0.06933011630095365
1108	14	0.02566295078688796
1108	15	0.11695514589440847
1108	16	0.15792173617472116
1108	17	0.11688216087078018
1108	18	0.17316770775084223
1108	19	0.1479650883020298
1108	20	0.13434330821036156
1108	21	0.17392189694704996
1108	22	0.17939216289700965
1108	23	0.022635615141574306
1108	24	0.09153717643637549
1108	25	0.3039685885480523
1109	1	0.051526994417198324
1109	2	0.07915293817416824
1109	3	0.04851223681382422
1109	4	0.0773527339564879
1109	5	0.11654963275478991
1109	6	0.14899929105253307
1109	7	0.1277383509687411
1109	8	0.12491577943826115
1109	9	0.14924047539717833
1109	10	0.13637003258697433
1109	11	0.1305410136921381
1109	12	0.10765412612766312
1109	13	0.1419433724375878
1109	14	0.17446784076101807
1109	15	0.24756927430259879
1109	16	0.11203510675868955
1109	17	0.12079029237668237
1109	18	0.0024134791011292123
1109	19	0.07142165982357085
1109	20	0.04946408273038827
1109	21	0.13086006416853024
1109	22	0.167034073299007
1109	23	0.20250599414730885
1109	24	0.12334333184747237
1109	25	0.2508788489393936
1110	1	0.13392424398486114
1110	2	0.018701849406230764
1110	3	0.07931914663824813
1110	4	0.20349560326877736
1110	5	0.20547222846290225
1110	6	0.20790193008144797
1110	7	0.17910543725222539
1110	8	0.10978464677059208
1110	9	0.21336966367393018
1110	10	0.22704688077147495
1110	11	0.25572429176484945
1110	12	0.3328462701598056
1110	13	0.23066175334137284
1110	14	0.19979578614935178
1110	15	0.23703122138969235
1110	16	0.2756024975614153
1110	17	0.3017328298771247
1110	18	0.3452948166700551
1110	19	0.2936497449003852
1110	20	0.28005245327125294
1110	21	0.24529075295929914
1110	22	0.2448626410413013
1110	23	0.28298669124990994
1110	24	0.3272489523860642
1110	25	0.5276020995044775
1111	1	0.17523459585010315
1111	2	0.14047081958418417
1111	3	0.12629612739026896
1111	4	0.13616615628976092
1111	5	0.2210905165659085
1111	6	0.2846507996089258
1111	7	0.28546095356688284
1111	8	0.3081606545761062
1111	9	0.3316428671493057
1111	10	0.34263229633482695
1111	11	0.3001949248899649
1111	12	0.34897793456303816
1111	13	0.3608413019429193
1111	14	0.37574494543919457
1111	15	0.36865929139247555
1111	16	0.3307382248492132
1111	17	0.31915022602510845
1111	18	0.37333397169654603
1111	19	0.3861613803677173
1111	20	0.44646786617656886
1111	21	0.34016677508352444
1111	22	0.3624660675296312
1111	23	0.3795439965567411
1111	24	0.3766534673386275
1111	25	0.5045710416096796
1112	1	-0.08673247632422587
1112	2	-0.06487059964192099
1112	3	-0.10510328508967248
1112	4	-0.08593152564080242
1112	5	-0.09003179421553398
1112	6	-0.030906647394694176
1112	7	-0.11545329119756786
1112	8	-0.05278013108590329
1112	9	0.013433778694732723
1112	10	-0.11866939014506528
1112	11	-0.08444957034038225
1112	12	-0.10209271475812187
1112	13	-0.1009011589192994
1112	14	-0.05009347301557909
1112	15	-0.1029335588003093
1112	16	-0.05323847135752402
1112	17	0.007935515578743946
1112	18	-0.03071341979271125
1112	19	-0.009110427666222165
1112	20	-0.1164335157341867
1112	21	-0.042032022477599185
1112	22	-0.09027795053402343
1112	23	-0.010017537621727968
1112	24	-0.05113190998832806
1112	25	0.12977520281873467
1113	1	-0.21082466871476058
1113	2	-0.16195722004334456
1113	3	-0.20896607018188362
1113	4	-0.25785954771363606
1113	5	-0.25281713390374705
1113	6	-0.20146225577749277
1113	7	-0.2048087636192264
1113	8	-0.21859623155399532
1113	9	-0.28137850041242984
1113	10	-0.31419148654326534
1113	11	-0.27756303002696114
1113	12	-0.20581662607193607
1113	13	-0.19806425984226905
1113	14	-0.27455052556193493
1113	15	-0.22512768264049754
1113	16	-0.21284770396044195
1113	17	-0.1915958829830018
1113	18	-0.26529783548961916
1113	19	-0.21143567348185144
1113	20	-0.19662231934753294
1113	21	-0.24667305279729845
1113	22	-0.1875029687927352
1113	23	-0.21244163225083668
1113	24	-0.13832979408116586
1113	25	-0.0348237294237137
1114	1	-0.28868308931562936
1114	2	-0.30064298476453644
1114	3	-0.34997402455800497
1114	4	-0.31064059008730993
1114	5	-0.3261246741200807
1114	6	-0.3388722995848852
1114	7	-0.3846241661736798
1114	8	-0.4420300065573741
1114	9	-0.3849209184699518
1114	10	-0.5050639236669241
1114	11	-0.44653514194966704
1114	12	-0.4546639034439153
1114	13	-0.33501973255238227
1114	14	-0.37673923201553805
1114	15	-0.5104283030373367
1114	16	-0.5494340581968515
1114	17	-0.42618974365477413
1114	18	-0.4237860432747951
1114	19	-0.5149272290230229
1114	20	-0.446778019781469
1114	21	-0.3572015936813884
1114	22	-0.3565617245512911
1114	23	-0.47614860841692774
1114	24	-0.5164315815963221
1114	25	-0.44204054286795724
1115	1	-0.6958118460364311
1115	2	-0.6448222154518923
1115	3	-0.6005439596691815
1115	4	-0.5991027653796195
1115	5	-0.7684606852035074
1115	6	-0.609015603052093
1115	7	-0.6752920725462062
1115	8	-0.6701548251567218
1115	9	-0.8337766925318775
1115	10	-0.837077073308059
1115	11	-0.8365802187770157
1115	12	-0.763587122634533
1115	13	-0.8759803707432852
1115	14	-1.0581123518647573
1115	15	-0.9310063338153185
1115	16	-0.9926012255258101
1115	17	-1.009131769624581
1115	18	-1.0559240847685984
1115	19	-0.9880854774254999
1115	20	-0.9832225914298007
1115	21	-1.0341136541751794
1115	22	-1.0677730679943096
1115	23	-1.0986976908646184
1115	24	-1.1575675152933371
1115	25	-1.0331661900193652
1116	1	-1.5022322711822689
1116	2	-1.518545821040174
1116	3	-1.408747757487454
1116	4	-1.3862576622119391
1116	5	-1.734531240416473
1116	6	-1.6154062580658817
1116	7	-1.4831287054025069
1116	8	-1.6726413399957138
1116	9	-1.9575977598619971
1116	10	-2.0129405843407255
1116	11	-2.0013309648023494
1116	12	-1.824600299462034
1116	13	-1.7865126181756068
1116	14	-2.0336639853235496
1116	15	-2.064195069364402
1116	16	-2.062115901492099
1116	17	-2.133216515267158
1116	18	-2.0114063963484696
1116	19	-2.190997279865229
1116	20	-2.148241268643602
1116	21	-2.182112321607363
1116	22	-2.3251429477088053
1116	23	-2.3780294222813225
1116	24	-2.481575709167778
1116	25	-2.446354358518313
1201	1	0.44120320400316615
1201	2	0.3983517536628156
1201	3	0.4094178836509528
1201	4	0.38784966638544915
1201	5	0.4760028689373357
1201	6	0.407994576677261
1201	7	0.49615958518741365
1201	8	0.519858110273681
1201	9	0.5789160033161806
1201	10	0.5410906251047223
1201	11	0.5122354119370343
1201	12	0.5764708596158101
1201	13	0.5532454664655333
1201	14	0.6282110218261252
1201	15	0.6155560329566683
1201	16	0.5736795258508849
1201	17	0.689610078729153
1201	18	0.687862684387575
1201	19	0.6913629137924673
1201	20	0.7594251747413523
1201	21	0.7250216563195195
1201	22	0.7049501950851536
1201	23	0.6792580141607161
1201	24	0.651986901016663
1201	25	0.6744357537029941
1202	1	0.4387129827058942
1202	2	0.4196305740726949
1202	3	0.4462122614965729
1202	4	0.3469483025540825
1202	5	0.2959186187402878
1202	6	0.3745050905890537
1202	7	0.39384171104597954
1202	8	0.5070854089740777
1202	9	0.5298769257061622
1202	10	0.5750986123041244
1202	11	0.5480390156771833
1202	12	0.6293271420787789
1202	13	0.5781264581845846
1202	14	0.6346163096238158
1202	15	0.4839050900377657
1202	16	0.572642287027918
1202	17	0.6608194987235336
1202	18	0.5895238863084202
1202	19	0.47827437253416605
1202	20	0.5589724886365985
1202	21	0.5865727596099504
1202	22	0.5965737440227841
1202	23	0.5793970916324085
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2210	24	0.10030252420060037
2210	25	-0.02277473079180936
2211	1	0.020367004874366046
2211	2	0.07302665249357077
2211	3	0.05435742203128413
2211	4	0.07950926853306584
2211	5	0.16955622871596177
2211	6	0.05036349652563388
2211	7	0.1112471985529524
2211	8	0.027562301532874756
2211	9	0.03746687051921782
2211	10	0.047277877513323574
2211	11	0.00927631150356234
2211	12	0.035039987934133876
2211	13	-0.029077040825583822
2211	14	0.01937317519816162
2211	15	-9.204915132272617E-4
2211	16	0.13034050091447114
2211	17	0.07003756210753664
2211	18	0.06290133930314568
2211	19	0.10351475296668866
2211	20	0.025805861679188524
2211	21	0.045064896507838625
2211	22	0.009364586443510348
2211	23	0.06007744505372159
2211	24	-0.0398870359731589
2211	25	-0.10638407706845499
2212	1	-0.09259043057506133
2212	2	-0.069016154728331
2212	3	-0.12486020561383526
2212	4	-0.01083119933725385
2212	5	0.028089765893213325
2212	6	0.017321368783441926
2212	7	-0.029647773297170943
2212	8	-0.044187116055944387
2212	9	-0.006290409620561377
2212	10	-0.07296212442804517
2212	11	-0.04594112200449274
2212	12	-0.09617239801033861
2212	13	-0.026453763791998597
2212	14	-0.06540890595113069
2212	15	-0.010002465887133383
2212	16	-0.0035570432517104678
2212	17	-0.015684870429836906
2212	18	-0.03346223087740441
2212	19	0.03184784362869664
2212	20	-0.056712095998932455
2212	21	-0.06407167891440935
2212	22	-0.13888453993971694
2212	23	-0.23961314900568453
2212	24	-0.14758942385900298
2212	25	-0.1624059660220567
2213	1	-0.10884894554917324
2213	2	-0.049912459265566866
2213	3	-0.051709737200248185
2213	4	-0.025567640186082485
2213	5	-0.03244310406711293
2213	6	-0.05488120119056106
2213	7	-0.09818260368177079
2213	8	-0.060998564285618784
2213	9	-0.1538192219166845
2213	10	-0.22368414202686182
2213	11	-0.18323364826949984
2213	12	-0.12603799463459353
2213	13	-0.007458530737338265
2213	14	-0.12620483100495505
2213	15	-0.21515677053958626
2213	16	-0.16961321507776717
2213	17	-0.15292892614980502
2213	18	-0.067074911168028
2213	19	-0.11164526698315314
2213	20	-0.043236924439305824
2213	21	-0.0507873291848
2213	22	-0.09080594474842485
2213	23	-0.1444247844567812
2213	24	-0.10922505497520518
2213	25	-0.12930661445623315
2214	1	-0.36932725633056407
2214	2	-0.4486098089260011
2214	3	-0.3698520976483586
2214	4	-0.26576347503686293
2214	5	-0.3394700063641167
2214	6	-0.4049867506963025
2214	7	-0.4396612905709745
2214	8	-0.49569539553607456
2214	9	-0.5702367998967617
2214	10	-0.5321888632028333
2214	11	-0.4997186423103912
2214	12	-0.536805419019089
2214	13	-0.4738559764509773
2214	14	-0.5856354612172296
2214	15	-0.6084538864602536
2214	16	-0.5315887446770873
2214	17	-0.4980143932592753
2214	18	-0.5460243899470498
2214	19	-0.5643886390975013
2214	20	-0.5613188236912308
2214	21	-0.5862112822477954
2214	22	-0.6373001583280029
2214	23	-0.6521636138943592
2214	24	-0.6617185132241374
2214	25	-1.4783883431911775
2215	1	-0.4853823200581253
2215	2	-0.4235467548471661
2215	3	-0.4896032240333241
2215	4	-0.32423360575436533
2215	5	-0.43956843375028143
2215	6	-0.41210525034905743
2215	7	-0.3994338695538602
2215	8	-0.4687823329305729
2215	9	-0.5661797441422465
2215	10	-0.5581114114155667
2215	11	-0.5414625123788213
2215	12	-0.5823354996146222
2215	13	-0.6299371268341858
2215	14	-0.6785253646558189
2215	15	-0.656701069571028
2215	16	-0.5537241555511798
2215	17	-0.6619081324619174
2215	18	-0.625021202951082
2215	19	-0.6139594944580509
2215	20	-0.6400157204790276
2215	21	-0.7113607460564211
2215	22	-0.6554806480799442
2215	23	-0.6461156742582119
2215	24	-0.6766669639659568
2215	25	-0.68690462912366
2216	1	-1.2289409481141433
2216	2	-0.9936745998770995
2216	3	-0.9760008161424025
2216	4	-1.0177754207713718
2216	5	-1.0766144816971135
2216	6	-1.0454586933957088
2216	7	-0.997670478462787
2216	8	-0.9876887601268152
2216	9	-1.1700998195701544
2216	10	-1.1517264126023719
2216	11	-1.236229701238699
2216	12	-1.2268336798503938
2216	13	-1.2645558334829161
2216	14	-1.314911088737368
2216	15	-1.252477700106148
2216	16	-1.2793096449095103
2216	17	-1.413105814240538
2216	18	-1.3406042527704187
2216	19	-1.455465613085579
2216	20	-1.4091275304850939
2216	21	-1.4085308808244648
2216	22	-1.5069044817165675
2216	23	-1.4744294912796008
2216	24	-1.4731688628503647
2216	25	-1.6575352169866235
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67559.0
3	6.0
4	1108.0
5	10102.0
6	11901.0
7	9212.0
8	7342.0
9	6485.0
10	5940.0
11	5651.0
12	5298.0
13	5060.0
14	5093.0
15	5245.0
16	5516.0
17	6031.0
18	6522.0
19	7655.0
20	9427.0
21	12426.0
22	15750.0
23	19519.0
24	23601.0
25	28200.0
26	32674.0
27	38113.0
28	45469.0
29	56619.0
30	72910.0
31	96600.0
32	127807.0
33	171589.0
34	236780.0
35	333248.0
36	496694.0
37	870081.0
38	1950939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57814002807823	18.42952793755161	13.082310899790734	25.91002113457942
2	17.238870186075726	20.555439247688838	36.270743369429425	25.934947196806007
3	18.780965386129918	24.191807320295247	28.24569223789899	28.781535055675846
4	13.424622222000119	16.747665201613472	35.736574440776955	34.091138135609455
5	15.59103765169407	35.38551014662357	33.00217542012307	16.02127678155929
6	33.32953274150906	36.69761305494969	15.8482806517904	14.12457355175085
7	29.494265171545663	30.37449852490195	21.01265938629931	19.118576917253073
8	27.10298135664411	32.729777385937844	19.358024794041444	20.809216463376597
9	27.12215523283261	14.827645012155836	18.45520018134968	39.59499957366187
10	16.45981344324841	26.260551469043964	30.389291348013725	26.8903437396939
11	37.174783039647345	21.841614835140497	21.744430073661185	19.239172051550973
12	25.109165674377476	23.781834182965788	27.875832909452903	23.233167233203833
13	29.815303046084946	19.91366102972653	24.429595838065783	25.841440086122745
14	23.871999594180142	20.039226480743515	24.672724581373533	31.416049343702806
15	25.283942958395507	26.702332395640525	22.34795478534139	25.66576986062258
16	25.698373035681282	26.141865666971796	23.089995199585196	25.069766097761725
17	25.19227370911442	25.33761462930893	24.081021689913037	25.389089971663616
18	26.561457584249283	24.044264903355295	24.988456439352596	24.40582107304283
19	27.19852845178925	24.51037093891861	24.26773928250383	24.023361326788315
20	26.2783581797876	24.39185992071643	24.193110462287915	25.136671437208065
21	26.76806714443238	24.471265868468628	24.032882011605416	24.72778497549358
22	25.82360880234636	24.244912655929976	24.775621329133347	25.15585721259032
23	24.78611973635848	23.656263149228884	25.048064849294242	26.50955226511839
24	25.10605238161269	25.203097717043825	24.561471712885513	25.129378188457967
25	25.166809751531954	24.426147808470013	24.772861450272664	25.63418098972537
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	378.0
1	378.0
2	592.5
3	807.0
4	807.0
5	807.0
6	2007.0
7	3207.0
8	3207.0
9	3207.0
10	4909.5
11	6612.0
12	6612.0
13	6612.0
14	10824.5
15	15037.0
16	15037.0
17	15037.0
18	28237.0
19	41437.0
20	41437.0
21	41437.0
22	63160.5
23	84884.0
24	84884.0
25	84884.0
26	118259.5
27	151635.0
28	151635.0
29	151635.0
30	185221.5
31	218808.0
32	218808.0
33	218808.0
34	267877.0
35	316946.0
36	316946.0
37	316946.0
38	355793.0
39	394640.0
40	394640.0
41	394640.0
42	446707.0
43	498774.0
44	498774.0
45	498774.0
46	532223.0
47	565672.0
48	565672.0
49	565672.0
50	577732.5
51	589793.0
52	589793.0
53	589793.0
54	553194.0
55	516595.0
56	516595.0
57	516595.0
58	484055.0
59	451515.0
60	451515.0
61	451515.0
62	400784.0
63	350053.0
64	350053.0
65	350053.0
66	296897.5
67	243742.0
68	243742.0
69	243742.0
70	192489.0
71	141236.0
72	141236.0
73	141236.0
74	117082.0
75	92928.0
76	92928.0
77	92928.0
78	79439.0
79	65950.0
80	65950.0
81	65950.0
82	50420.0
83	34890.0
84	34890.0
85	34890.0
86	25841.0
87	16792.0
88	16792.0
89	16792.0
90	11954.0
91	7116.0
92	7116.0
93	7116.0
94	3873.5
95	631.0
96	631.0
97	631.0
98	362.5
99	94.0
100	94.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030456291375859323
2	0.0029312881119427747
3	0.007608875524617415
4	0.15833113659968917
5	0.020498227506209755
6	0.08114054965186276
7	0.0489795375300509
8	0.019791392074961146
9	0.03704649230838315
10	0.023408726340762867
11	0.03752464568834545
12	0.029645509557662385
13	0.006091258275171865
14	0.003243127272787751
15	0.0028897095571634446
16	0.003554966433632726
17	0.002120506293745837
18	0.002723395338046124
19	0.0
20	0.0019957706294078466
21	8.315710955866027E-5
22	0.0
23	8.315710955866027E-5
24	0.0010186745920935884
25	0.13504714592326428
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4810172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.98428075103256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0782764686519	41.185138996033785
2	10.0217083010896	9.818123460510531
3	2.414897443414885	3.5487604305955656
4	0.9449196631394418	1.8514484026557414
5	0.5536535825002744	1.3560161262004207
6	0.33365785811544735	0.9806394118029157
7	0.23147010430172332	0.7936877602210485
8	0.1812133430045298	0.710128421565364
9	0.1274360256213479	0.5618125851148695
>10	0.8829150530117889	8.48526129630767
>50	0.10802003127246547	3.6921154715464937
>100	0.09499518376962768	9.516332570015768
>500	0.014250459090953401	4.882034064659203
>1k	0.012159822574579747	11.34627631383218
>5k	4.2666044121332346E-4	1.27222468893853
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	9867	0.20512780000382522	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7280	0.1513459393967617	No Hit
GAATAGGACCGCGGTTCTATTTTGT	6529	0.13573319207712323	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	5892	0.12249042237990658	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	5522	0.1147983897457305	No Hit
GTATCTGATCGTCTTCGAACCTCCG	5306	0.11030790582956286	No Hit
CTTATGACCCGCACTTACTGGGAAT	5153	0.10712714638894409	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	5138	0.10681530722809912	No Hit
ATCAGATACCGTCGTAGTTCCGACC	5101	0.1060461039646815	No Hit
CTATTGGAGCTGGAATTACCGCGGC	5085	0.10571347552644687	No Hit
GACTTAATCAACGCAAGCTTATGAC	4978	0.1034890228457527	No Hit
GAACTACGACGGTATCTGATCGTCT	4977	0.10346823356836304	No Hit
GTCCTATTCCATTATTCCTAGCTGC	4875	0.1013477272746172	No Hit
TCGTAGTTCCGACCATAAACGATGC	4872	0.10128535944244821	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	4858	0.10099430955899291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.1578554779330135E-5
2	0.0	0.0	0.0	0.0	4.1578554779330135E-5
3	0.0	0.0	0.0	0.0	4.1578554779330135E-5
4	0.0	0.0	0.0	4.1578554779330135E-5	4.1578554779330135E-5
5	0.0	0.0	0.0	4.1578554779330135E-5	4.1578554779330135E-5
6	2.0789277389665068E-5	0.0	0.0	4.1578554779330135E-5	6.23678321689952E-5
7	2.0789277389665068E-5	0.0	0.0	4.1578554779330135E-5	6.23678321689952E-5
8	2.0789277389665068E-5	0.0	0.0	6.23678321689952E-5	8.315710955866027E-5
9	2.0789277389665068E-5	0.0	0.0	1.0394638694832533E-4	8.315710955866027E-5
10	2.0789277389665068E-5	0.0	0.0	1.0394638694832533E-4	8.315710955866027E-5
11	2.0789277389665068E-5	0.0	0.0	1.0394638694832533E-4	8.315710955866027E-5
12	2.0789277389665068E-5	0.0	0.0	1.247356643379904E-4	1.4552494172765548E-4
13	2.0789277389665068E-5	0.0	0.0	1.247356643379904E-4	1.6631421911732054E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2530	0.0	15.837281	1
CTCGACG	175	0.0	14.103266	13
CGCTTAC	175	0.0	14.102679	18
GTATCAA	8140	0.0	13.2939825	1
ACGCTTA	190	0.0	12.989309	17
TCGACGC	210	0.0	11.752233	14
CGTCGTA	810	0.0	11.723139	10
AACCGCG	495	0.0	11.511546	7
ATACCGT	850	0.0	11.511513	6
CGACCAT	910	0.0	11.47837	10
CGGTTCT	1220	0.0	11.440758	12
GCTCGTA	465	0.0	11.435969	9
GGTAGCG	265	0.0	11.108227	11
TACCGTC	855	0.0	11.107632	7
CGGTAGC	275	0.0	11.04959	10
CGAGCCG	1135	0.0	11.039372	15
CCGGTAG	270	0.0	10.902745	9
AGGCCCG	1200	0.0	10.761841	10
GGTTCTA	1335	0.0	10.665823	13
ACCGTCG	925	0.0	10.573856	8
>>END_MODULE