FastQCFastQC Report
Thu 2 Feb 2017
SRR4050272_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050272_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972559
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA209612.1552419956012954No Hit
CTTCCGTACTCTGCGTTGATACCAC133251.3700968270305451No Hit
GTACATGGGAAGCAGTGGTATCAAC125611.2915411815632778No Hit
GAGTACGGAAGCAGTGGTATCAACG105121.0808598758532901No Hit
TCCGTACTCTGCGTTGATACCACTG100271.0309914359951429No Hit
CCCATGTACTCTGCGTTGATACCAC97741.0049775900485214No Hit
GCTTCCGTACTCTGCGTTGATACCA74160.7625244329649924No Hit
GCGTTGATACCACTGCTTCCGTACT52540.5402242948756836No Hit
GAGTACATGGGAAGCAGTGGTATCA41470.4264008661685307No Hit
GTATCAACGCAGAGTACGGAAGCAG40270.4140622831108447No Hit
ACTCTGCGTTGATACCACTGCTTCC36970.3801311797022083No Hit
GTACATGGAAGCAGTGGTATCAACG34000.34959318663443556No Hit
TATCAACGCAGAGTACGGAAGCAGT33390.3433210735801118No Hit
TCCATGTACTCTGCGTTGATACCAC30120.30969843474791764No Hit
CATGTACTCTGCGTTGATACCACTG28710.29520059965513656No Hit
GTATCAACGCAGAGTACATGGGAAG28300.29098491711042723No Hit
GCGTTGATACCACTGCTTCCCATGT27900.2868720560911986No Hit
GGTATCAACGCAGAGTACGGAAGCA23760.24430394454218202No Hit
ATACCACTGCTTCCGTACTCTGCGT23190.23844311758978118No Hit
TATCAACGCAGAGTACATGGGAAGC22460.2309371462296889No Hit
CAGTGGTATCAACGCAGAGTACGGA21910.2252819623282495No Hit
ACGCAGAGTACGGAAGCAGTGGTAT19640.20194147604412688No Hit
GTACTCTGCGTTGATACCACTGCTT19490.20039915316191614No Hit
GCTTCCCATGTACTCTGCGTTGATA18490.1901170006138445No Hit
ACGCAGAGTACATGGGAAGCAGTGG18290.18806057010423016No Hit
AAGCAGTGGTATCAACGCAGAGTAC18180.1869295333239423No Hit
GTGGTATCAACGCAGAGTACGGAAG15700.16142979500472465No Hit
GGTATCAACGCAGAGTACATGGGAA15270.15700846940905386No Hit
GAAGCAGTGGTATCAACGCAGAGTA15260.15690564788357314No Hit
GTATCAACGCAGAGTACTTTTTTTT14540.14950249804896154No Hit
CAGTGGTATCAACGCAGAGTACATG13340.1371639149912756No Hit
GTTGATACCACTGCTTCCGTACTCT12370.1271902270196461No Hit
GTACGGGAAGCAGTGGTATCAACGC12170.1251337965100318No Hit
GATACCACTGCTTCCGTACTCTGCG12000.1233858305768596No Hit
GATATACACTGTTCTACAAATCCCG11670.11999272023599597No Hit
GAGTACATGGAAGCAGTGGTATCAA11000.11310367802878796No Hit
CCACTGCTTCCGTACTCTGCGTTGA10750.11053313989177008No Hit
TTGTAGAACAGTGTATATCAATGAG10440.10734567260186785No Hit
ATACCACTGCTTCCCATGTACTCTG10280.1057005281941764No Hit
ATCAACGCAGAGTACGGAAGCAGTG10180.10467231293936922No Hit
CGGAAGCAGTGGTATCAACGCAGAG10000.10282152548071632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA701.2896635E-917.6634110
AGAACCA607.5748176E-817.4369565
CGGTCCA1350.016.90856410
CCGTCGT851.6878403E-814.5463389
CGCCGGT1500.013.9495667
TTTTTCG1101.8371793E-1013.82641415
TCGTCAC1051.3551471E-913.57951419
GTCGTAG852.657507E-713.42738911
TATACTG1009.889845E-913.3154945
TCTATAC500.00153924813.2531253
AGGCCCG1153.6925485E-1013.23278910
GATATAT655.5696128E-513.1230221
GTTTTCG957.318158E-813.00774515
GCCGGTC1700.012.8679148
GGTCCAA1850.012.85279411
GTCTAAA604.171297E-412.6369851
TATTGCG1052.0905645E-812.6220242
TTTTCGG1001.429089E-712.35735816
TAGACAG701.0782953E-412.2285165
CGAGGAT1405.0931703E-1112.22156216
Produced by FastQC (version 0.11.5)