##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050272_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	972559
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.33914343499983	31.0	31.0	34.0	27.0	34.0
2	30.47671041037099	31.0	31.0	34.0	27.0	34.0
3	30.575792316969974	31.0	31.0	34.0	27.0	34.0
4	34.16068125429923	37.0	35.0	37.0	30.0	37.0
5	34.18700253660703	37.0	35.0	37.0	30.0	37.0
6	34.16022267029558	37.0	35.0	37.0	30.0	37.0
7	34.04063301043947	37.0	35.0	37.0	30.0	37.0
8	34.01253497217135	37.0	35.0	37.0	30.0	37.0
9	35.529644988118974	39.0	37.0	39.0	30.0	39.0
10	35.41704410734979	39.0	35.0	39.0	30.0	39.0
11	35.260648454232594	39.0	35.0	39.0	30.0	39.0
12	35.20144073521504	39.0	35.0	39.0	29.0	39.0
13	35.21627993777241	39.0	35.0	39.0	29.0	39.0
14	36.36347614900484	40.0	37.0	41.0	29.0	41.0
15	36.33616983648293	40.0	37.0	41.0	29.0	41.0
16	36.28327535912988	40.0	37.0	41.0	29.0	41.0
17	36.27524088512882	40.0	37.0	41.0	29.0	41.0
18	36.2436345764113	40.0	37.0	41.0	29.0	41.0
19	36.25070766914912	40.0	37.0	41.0	29.0	41.0
20	36.21785824818854	40.0	37.0	41.0	29.0	41.0
21	36.13081057293182	40.0	37.0	41.0	28.0	41.0
22	36.08777667987238	40.0	37.0	41.0	29.0	41.0
23	36.014703478143744	40.0	37.0	41.0	28.0	41.0
24	35.938701919369414	40.0	37.0	41.0	28.0	41.0
25	35.73919936990969	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7661282654931654
1101	2	0.6370747254844638
1101	3	0.5502599029482376
1101	4	0.6793488844784719
1101	5	0.6739777804070215
1101	6	0.6295090773924983
1101	7	0.46385624492290845
1101	8	0.4770838898698244
1101	9	0.7457886836533021
1101	10	0.6953106948132017
1101	11	0.5173248499308798
1101	12	0.5385795186770821
1101	13	0.5775741624060657
1101	14	0.8748634315603994
1101	15	0.7949169655519839
1101	16	0.7694653229978741
1101	17	0.8580186520007658
1101	18	0.8890304587724316
1101	19	0.7609143331787109
1101	20	0.8612914867234807
1101	21	0.8911674273645431
1101	22	0.9851926130079107
1101	23	0.993016839804028
1101	24	0.9095075612877466
1101	25	0.964131588174773
1102	1	0.7536351700668007
1102	2	0.7821131733592317
1102	3	0.7878459952460553
1102	4	0.8521385614246455
1102	5	0.8115627267175114
1102	6	0.7950629903662261
1102	7	0.7674131603834198
1102	8	0.7428378922305612
1102	9	0.8498392904535947
1102	10	0.9296774795165632
1102	11	0.7897315379129068
1102	12	0.872615113043409
1102	13	0.9391625008390392
1102	14	1.1602624070477319
1102	15	1.1224800235045649
1102	16	1.0762406292629905
1102	17	1.1849055774313726
1102	18	1.082017474736034
1102	19	1.1124445700542935
1102	20	1.1754272403820494
1102	21	1.2392784964561159
1102	22	1.281789259848999
1102	23	1.295240742807664
1102	24	1.2750445136490498
1102	25	1.4395650578805999
1103	1	0.5061706670242039
1103	2	0.5684552964153511
1103	3	0.726317935746394
1103	4	0.7006848765024003
1103	5	0.6668703670150151
1103	6	0.688234051894284
1103	7	0.7349587993286519
1103	8	0.4615667630892446
1103	9	0.7166411836246525
1103	10	0.8465131793137175
1103	11	1.0175881978182488
1103	12	0.9929667019548347
1103	13	0.7971746712244396
1103	14	0.9513374577576741
1103	15	0.9656172784065262
1103	16	0.9024745711155902
1103	17	0.7684079438951983
1103	18	0.9616658858246083
1103	19	0.9508488973024356
1103	20	0.8495313647904297
1103	21	0.9850526681937737
1103	22	0.9241151557245928
1103	23	1.0443955771551927
1103	24	0.9592906258870499
1103	25	0.9723574851963548
1104	1	0.6306228195921619
1104	2	0.544036107017547
1104	3	0.4941436932496188
1104	4	0.6092961969577573
1104	5	0.6759847063587685
1104	6	0.6218951020562073
1104	7	0.562252304216365
1104	8	0.636288434025623
1104	9	0.6869766100965009
1104	10	0.7487543557387184
1104	11	0.8266835708013076
1104	12	0.8097037857948095
1104	13	0.9589486095299691
1104	14	0.8406154001993258
1104	15	0.9540076772335269
1104	16	1.063761584583986
1104	17	0.9321464506681494
1104	18	1.036606718529903
1104	19	0.9583697887943359
1104	20	1.0185494498536443
1104	21	0.930083504487186
1104	22	0.9651878128433324
1104	23	1.042226755572571
1104	24	1.1193917858897322
1104	25	1.2239644967316394
1105	1	0.38556284535147256
1105	2	0.42707786421641813
1105	3	0.43409319144254255
1105	4	0.47970759670158714
1105	5	0.3852973929978205
1105	6	0.5712430026010011
1105	7	0.5922532496758421
1105	8	0.5273246754495915
1105	9	0.6351672147267493
1105	10	0.8081171695688241
1105	11	0.801205667794747
1105	12	0.7982405356262348
1105	13	0.8701003932373794
1105	14	0.8025896444561056
1105	15	0.7982624054228467
1105	16	0.7565131214016034
1105	17	0.7773693828769197
1105	18	0.7856621509973394
1105	19	0.768319947025006
1105	20	0.8231715871629035
1105	21	0.7566902504297346
1105	22	0.8647657454623996
1105	23	0.8077652928575674
1105	24	0.9622983423836118
1105	25	0.8826411388877133
1106	1	0.054700968436964814
1106	2	0.17754082717938147
1106	3	0.09574384585635443
1106	4	0.037410882694352665
1106	5	0.12430115239631334
1106	6	0.1081999783737686
1106	7	0.10402573644832813
1106	8	0.2014683680143392
1106	9	0.14284265332324253
1106	10	0.15308723361783905
1106	11	0.16206615233944177
1106	12	0.13968790404728537
1106	13	0.1919675611571776
1106	14	0.34060830221160643
1106	15	0.307410275097034
1106	16	0.3173979724180356
1106	17	0.2336698562826598
1106	18	0.23282171657795914
1106	19	0.2326404705560563
1106	20	0.16298640197771874
1106	21	0.11773522397471226
1106	22	0.25969751934154317
1106	23	0.21530497539725246
1106	24	0.22600622319675523
1106	25	0.2234201697983238
1107	1	0.3252145812360858
1107	2	0.45568009658577324
1107	3	0.4590352078229145
1107	4	0.4223762927949437
1107	5	0.4404996108712709
1107	6	0.40732149792607686
1107	7	0.28801138550247174
1107	8	0.16770845453200423
1107	9	0.21279278550300518
1107	10	0.31736736888751693
1107	11	0.4709436543135581
1107	12	0.6801713901598276
1107	13	0.6339621023045652
1107	14	0.4854464249156294
1107	15	0.5876459670666847
1107	16	0.7753034381371293
1107	17	0.6513999692362731
1107	18	0.5365862649351527
1107	19	0.7000804746759428
1107	20	0.7805989264395592
1107	21	0.8526259610914764
1107	22	0.6995849090486885
1107	23	0.8813682500221702
1107	24	0.9408987627648528
1107	25	0.9869029349303347
1108	1	-0.1738215710957256
1108	2	-0.02884129966238902
1108	3	-0.09619662752440661
1108	4	-0.17718375974133238
1108	5	-0.15786404197975656
1108	6	-0.23124293945893015
1108	7	-0.27339258514039244
1108	8	-0.30185355787661905
1108	9	-0.18812981709046284
1108	10	-0.2675258180696787
1108	11	-0.33845729754086307
1108	12	-0.5669100088965067
1108	13	-0.3865726795791886
1108	14	-0.3251626273544588
1108	15	-0.23037842310586854
1108	16	-0.21131970181238557
1108	17	-0.06734079815613114
1108	18	-0.13742846043408008
1108	19	-0.17942247829589775
1108	20	-0.28185664734963467
1108	21	-0.1921702853142051
1108	22	-0.4242702476094635
1108	23	-0.3927827888564295
1108	24	-0.14460724994798824
1108	25	-0.15305535196572606
1109	1	-0.31592276398315633
1109	2	-0.14383472556856702
1109	3	-0.11649598856861942
1109	4	-0.09701866480041588
1109	5	-0.08333134087096283
1109	6	-0.19357089305622566
1109	7	-0.2102193677841413
1109	8	-0.24663400256783774
1109	9	-0.24091195983631764
1109	10	-0.29523332694338933
1109	11	-0.04552020933226686
1109	12	-0.22260595837250463
1109	13	-0.10132216188700482
1109	14	-0.2275766896426319
1109	15	-0.08785271655771965
1109	16	-0.08874168579255581
1109	17	-0.07149771362125534
1109	18	-0.12515115305664182
1109	19	-0.018203197362957724
1109	20	0.0921083223820176
1109	21	-0.024739507547714368
1109	22	0.014879219644100772
1109	23	0.081903206603819
1109	24	0.10090814160616901
1109	25	-8.180354748077434E-4
1110	1	-0.2911590289510464
1110	2	-0.2653561951531245
1110	3	-0.36468941782393927
1110	4	-0.2505266254681189
1110	5	-0.3261967580124576
1110	6	-0.5161127420128082
1110	7	-0.41756235288169563
1110	8	-0.3354357719882728
1110	9	-0.30941508469425827
1110	10	-0.44471706310411463
1110	11	-0.3570764622615741
1110	12	-0.3754567348452298
1110	13	-0.29027417842751646
1110	14	-0.3569298981279516
1110	15	-0.3882701480451871
1110	16	-0.3351046395709929
1110	17	-0.3605282630852429
1110	18	-0.12498813062067882
1110	19	-0.1900609790978578
1110	20	-0.17063642680639646
1110	21	-0.2167250737920412
1110	22	-0.38045528201669043
1110	23	-0.503491550657337
1110	24	-0.4040479755756081
1110	25	-0.41679956377879535
1111	1	-0.5035043274113526
1111	2	-0.5507976821373894
1111	3	-0.5407917867033056
1111	4	-0.5475065739908374
1111	5	-0.5126839734618045
1111	6	-0.6078632775922301
1111	7	-0.5826202262845896
1111	8	-0.6377029297954024
1111	9	-0.8041702593933309
1111	10	-0.8000551331686978
1111	11	-0.7603022687131897
1111	12	-0.7682190917029601
1111	13	-0.7984296274665539
1111	14	-0.6451386739243858
1111	15	-0.7759094372272557
1111	16	-0.8531602165326007
1111	17	-0.6842363222070418
1111	18	-0.7092998917907849
1111	19	-0.7887308926345327
1111	20	-0.6587527284570598
1111	21	-0.7988020431060363
1111	22	-0.8441599504198152
1111	23	-1.0196329128015051
1111	24	-0.788483806224626
1111	25	-0.7387529259846133
1112	1	-0.36787266748770975
1112	2	-0.4806778272548691
1112	3	-0.40962191947440374
1112	4	-0.37127051115724896
1112	5	-0.4247834283215326
1112	6	-0.4156471969038762
1112	7	-0.3786959760898938
1112	8	-0.4470709849552392
1112	9	-0.7033648953649916
1112	10	-0.4801394684584537
1112	11	-0.4272747457774102
1112	12	-0.5210255194196378
1112	13	-0.5829358671383318
1112	14	-0.5629326602863785
1112	15	-0.5404772188672311
1112	16	-0.4832398032137064
1112	17	-0.5281400584123332
1112	18	-0.7072313978595091
1112	19	-0.5953371569990722
1112	20	-0.5679004481751875
1112	21	-0.588189852477889
1112	22	-0.6657813153598866
1112	23	-0.6781965536547361
1112	24	-0.6946721930272872
1112	25	-0.5944958932951891
1113	1	-0.6339606206005648
1113	2	-0.5721576885905648
1113	3	-0.5614661434025052
1113	4	-0.4673823503883554
1113	5	-0.5076370535080557
1113	6	-0.738255039039899
1113	7	-0.7262463723952095
1113	8	-0.7722784991535789
1113	9	-0.9050306826140044
1113	10	-0.9133406054603768
1113	11	-0.7562824789791165
1113	12	-0.8220439418010983
1113	13	-0.8676856302629261
1113	14	-0.8157794203415278
1113	15	-0.8568860175827169
1113	16	-0.6244220809621694
1113	17	-0.7715208802990574
1113	18	-0.8631731406950962
1113	19	-0.8303538659345264
1113	20	-0.8102974570204253
1113	21	-0.7155749182640037
1113	22	-1.009143414161045
1113	23	-0.9163122169342302
1113	24	-0.7136619407195255
1113	25	-0.7344481210586906
1114	1	-0.27403483210334656
1114	2	-0.22892948821950654
1114	3	-0.2306065268286055
1114	4	-0.3529729502646717
1114	5	-0.30850285437385594
1114	6	-0.47480420416049185
1114	7	-0.34467556225296647
1114	8	-0.3344306327342892
1114	9	-0.3738000085261888
1114	10	-0.4210709703336022
1114	11	-0.18485390755054
1114	12	-0.28524740500456147
1114	13	-0.26409874096175656
1114	14	-0.48244608700819924
1114	15	-0.29349702562229396
1114	16	-0.347984233095751
1114	17	-0.30831741709559424
1114	18	-0.23608594217932222
1114	19	-0.27624131182196976
1114	20	-0.3310148348806621
1114	21	-0.44841350824545145
1114	22	-0.49213661143995324
1114	23	-0.4147661439595822
1114	24	-0.5157645999650455
1114	25	-0.3615353195744575
1115	1	-0.5976814467449607
1115	2	-0.6360616371526575
1115	3	-0.5231644413987482
1115	4	-0.6310844070865187
1115	5	-0.6384889697963274
1115	6	-0.8553751803401397
1115	7	-0.7937044840445395
1115	8	-0.7315151120866901
1115	9	-0.741616183457424
1115	10	-0.766571958135863
1115	11	-0.7771634879090428
1115	12	-0.8009812542570316
1115	13	-0.8178744075778894
1115	14	-0.821628027913313
1115	15	-0.950185806546763
1115	16	-1.1181343164754054
1115	17	-0.9687961823547937
1115	18	-0.8675093640418794
1115	19	-0.9676547714399888
1115	20	-0.9023832478277285
1115	21	-1.0688892896439697
1115	22	-1.044371017759964
1115	23	-1.0907585784289466
1115	24	-0.793774990537969
1115	25	-0.8915544018447932
1116	1	-0.2600073956596489
1116	2	-0.31862650748995947
1116	3	-0.24256303105536503
1116	4	-0.10150774403278717
1116	5	-0.2130063573600367
1116	6	-0.2962101576878311
1116	7	-0.278439193411387
1116	8	-0.4542615127999241
1116	9	-0.31807259823249723
1116	10	-0.2943642482933555
1116	11	-0.29336840097771955
1116	12	-0.35105806156815333
1116	13	-0.2722318903774692
1116	14	-0.5933543599548301
1116	15	-0.4999650017724946
1116	16	-0.3815275101259985
1116	17	-0.4088072030992649
1116	18	-0.5385895269617507
1116	19	-0.6475541291697127
1116	20	-0.7289975659581529
1116	21	-0.90227980608541
1116	22	-0.8356721674003609
1116	23	-1.0868493332199805
1116	24	-1.3239858662452875
1116	25	-1.278411124368958
1201	1	0.4504622911750893
1201	2	0.5482161826296164
1201	3	0.33813729987053875
1201	4	0.196124758616385
1201	5	0.4832154701812996
1201	6	0.5607393540641823
1201	7	0.3670250594002624
1201	8	0.41819769132261086
1201	9	0.4651153077080181
1201	10	0.5042573686711123
1201	11	0.4756489838257423
1201	12	0.4319575295328022
1201	13	0.378789176807679
1201	14	0.5807216186781261
1201	15	0.35533191569140854
1201	16	0.3631419235280404
1201	17	0.31823048771907025
1201	18	0.3952687108408668
1201	19	0.4467346515060555
1201	20	0.38519357868726445
1201	21	0.5620734094858832
1201	22	0.5951000066023511
1201	23	0.41142887519413307
1201	24	0.4222459338477904
1201	25	0.5298614804208484
1202	1	0.7797813827029856
1202	2	0.7077876371189369
1202	3	0.5770325065611743
1202	4	0.7867671267295577
1202	5	0.7301956948303072
1202	6	0.6603954645707901
1202	7	0.6363628754678032
1202	8	0.7888203527444659
1202	9	0.8392191511494076
1202	10	0.834800057484081
1202	11	0.867971740689093
1202	12	0.7424984268283836
1202	13	0.8070986982679216
1202	14	0.8364939195457382
1202	15	0.8292555615839063
1202	16	0.8688759159336428
1202	17	1.028018765829863
1202	18	0.9655900881448858
1202	19	0.9950958450567811
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2210	13	-0.2022274852824495
2210	14	-0.41697683710727773
2210	15	-0.35412223264290077
2210	16	-0.14308964368103716
2210	17	-0.24829608798978597
2210	18	-0.23915970757064997
2210	19	-0.21619853873187367
2210	20	-0.1497070964742946
2210	21	-0.20111152525129938
2210	22	-0.12420340945102737
2210	23	-0.153348275376743
2210	24	-0.30348294088992844
2210	25	-0.1883058363936243
2211	1	-0.3681098653597701
2211	2	-0.20963288319973827
2211	3	-0.31576910759626386
2211	4	-0.20031318586735836
2211	5	-0.23568502731648522
2211	6	-0.1544455844144963
2211	7	-0.07767096958026087
2211	8	-0.195581941102148
2211	9	-0.3375901915297206
2211	10	-0.267676316805769
2211	11	-0.31499462421349733
2211	12	-0.23842820272056287
2211	13	-0.3960513129391856
2211	14	-0.31468265981122556
2211	15	-0.36288101403318507
2211	16	-0.35878656521566654
2211	17	-0.2121472579759498
2211	18	-0.3951018057144111
2211	19	-0.5864486989182822
2211	20	-0.5769763077798942
2211	21	-0.38466533537892644
2211	22	-0.4785576898436332
2211	23	-0.5684271563616079
2211	24	-0.455267696531088
2211	25	-0.57156486503861
2212	1	-0.5329829810523812
2212	2	-0.47070002836928637
2212	3	-0.5285068964675261
2212	4	-0.5548856716176545
2212	5	-0.4724322146542903
2212	6	-0.493571402185502
2212	7	-0.5568236125907262
2212	8	-0.42450888913970886
2212	9	-0.4056470030868482
2212	10	-0.348593599742685
2212	11	-0.36073624267475424
2212	12	-0.5656776292838686
2212	13	-0.5363590287211153
2212	14	-0.7437816870206149
2212	15	-0.6511036088067712
2212	16	-0.5991872561524616
2212	17	-0.6530980165923381
2212	18	-0.6148387395841439
2212	19	-0.6643763663284687
2212	20	-0.5060774541909083
2212	21	-0.6283357617661451
2212	22	-0.5780484264280261
2212	23	-0.7672895689300887
2212	24	-0.8786475765226314
2212	25	-0.7778209794169371
2213	1	-0.8156708886003834
2213	2	-0.8394436145533852
2213	3	-1.0659798463821737
2213	4	-1.0388980829779157
2213	5	-1.0075270644239538
2213	6	-1.0477186658573103
2213	7	-1.1277314325571623
2213	8	-1.08297898435071
2213	9	-1.1343060413236827
2213	10	-1.1047091602038108
2213	11	-1.0016079429047693
2213	12	-1.2536203551608338
2213	13	-1.3282493554479373
2213	14	-1.3909642227554784
2213	15	-1.429551447391006
2213	16	-1.3949350451472284
2213	17	-1.2242899073729703
2213	18	-1.2558507730659159
2213	19	-1.1942766162427034
2213	20	-1.3230416639161149
2213	21	-1.2112687418581132
2213	22	-1.4905096031015361
2213	23	-1.5008481667626015
2213	24	-1.5488235831678452
2213	25	-1.6302490283648723
2214	1	-0.45936660791131345
2214	2	-0.40656411109222645
2214	3	-0.35166518598250107
2214	4	-0.4637668301061666
2214	5	-0.36880313610271287
2214	6	-0.30014147711681005
2214	7	-0.3749866092306817
2214	8	-0.39926262613770547
2214	9	-0.48335130379176405
2214	10	-0.4549780685264153
2214	11	-0.5244997995773844
2214	12	-0.48632736560833223
2214	13	-0.5226685661982486
2214	14	-0.361211061011268
2214	15	-0.4374077885806784
2214	16	-0.4176491360939849
2214	17	-0.41751597867510526
2214	18	-0.4453784133765595
2214	19	-0.3472003351619435
2214	20	-0.32775433876302174
2214	21	-0.6966166278771411
2214	22	-0.6056046249079685
2214	23	-0.39241107398546404
2214	24	-0.6578956611437263
2214	25	-0.5573059510593765
2215	1	-0.6436011271436435
2215	2	-0.5427778324789649
2215	3	-0.5963691979226056
2215	4	-0.6100517703245316
2215	5	-0.7309265604468962
2215	6	-0.512384951427741
2215	7	-0.49603996657400984
2215	8	-0.7955248542002025
2215	9	-0.834390384922834
2215	10	-0.8896862984383205
2215	11	-0.7754139638511646
2215	12	-0.7983429072121382
2215	13	-0.9570693708574822
2215	14	-0.9592464595284866
2215	15	-0.9542796757986025
2215	16	-1.0881444233216442
2215	17	-1.0836878448031726
2215	18	-1.221825907739074
2215	19	-1.1155769027062732
2215	20	-1.1910214823849614
2215	21	-1.2741997300331747
2215	22	-1.1901814487751992
2215	23	-1.4387357604765967
2215	24	-1.4279480613319109
2215	25	-1.5774573113552819
2216	1	-0.4305242768797193
2216	2	-0.4934337490918068
2216	3	-0.4582146733229493
2216	4	-0.49310874264017457
2216	5	-0.6086493382370506
2216	6	-0.6495163463363127
2216	7	-0.45910985677200955
2216	8	-0.5252042457233941
2216	9	-1.0219655536348284
2216	10	-0.6592232034918837
2216	11	-0.5007683704081032
2216	12	-0.6032425152212255
2216	13	-0.4995021087803764
2216	14	-0.7175094497955286
2216	15	-0.5473287971598779
2216	16	-0.7643482363241603
2216	17	-1.0191847378297396
2216	18	-0.920333948007233
2216	19	-0.7597212600416299
2216	20	-0.7401915641239754
2216	21	-0.9021800302625778
2216	22	-0.9085785734327629
2216	23	-1.0902034991790188
2216	24	-1.0327805743054768
2216	25	-1.1892582423337004
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25070.0
3	2.0
4	215.0
5	1401.0
6	2261.0
7	2326.0
8	1939.0
9	1636.0
10	1626.0
11	2298.0
12	2448.0
13	2138.0
14	1211.0
15	1166.0
16	1214.0
17	1353.0
18	1474.0
19	1962.0
20	2392.0
21	3197.0
22	3937.0
23	4419.0
24	5192.0
25	6003.0
26	6789.0
27	7485.0
28	8675.0
29	10477.0
30	12815.0
31	16926.0
32	21807.0
33	29000.0
34	40600.0
35	58035.0
36	89118.0
37	167845.0
38	426107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.00241216008833	16.1155364944784	12.515011628218359	24.367039717214915
2	13.484439133104264	21.260501695389383	39.818767657092195	25.43629151441415
3	20.004834147595783	27.681563383903317	28.04227307791206	24.271329390588843
4	12.856885688568855	16.047421885045647	35.31233123312331	35.78336119326218
5	15.262115271475063	35.76221606921719	32.18605617519882	16.789612484108925
6	33.97702426702473	33.85026160233359	16.456201544405516	15.716512586236167
7	26.94667218198918	31.6556874705455	22.243213375068684	19.154426972396635
8	26.290844057353013	34.163608786115745	21.860021444609316	17.685525711921926
9	28.72213357366181	16.43451717741612	17.261057836241246	37.582291412680824
10	14.979390171266802	27.55081991834249	31.02789913476781	26.441890775622902
11	34.05262629700864	24.038462533275535	20.391577888802555	21.517333280913267
12	25.306809041225627	24.305169280014557	28.392475708581554	21.995545970178267
13	30.46640811381838	20.059621515014946	25.64293658701971	23.831033784146964
14	26.392575137459172	20.794679399727148	24.6061226177188	28.206622845094877
15	29.693333719233106	23.881166299372776	23.72921826353371	22.696281717860405
16	24.982819254422814	24.670827249024185	28.356032586061325	21.990320910491672
17	24.480469282888453	27.05579968664867	26.470746297038872	21.992984733424002
18	25.871523414246973	22.957247503141744	30.62227164495006	20.548957437661223
19	25.007674680376695	28.418333648589567	23.488966548729827	23.08502512230391
20	22.969419857868694	27.248862081367903	26.630293233673296	23.15142482709011
21	24.07180974489949	28.31759347018763	24.013515231508805	23.597081553404077
22	22.57431779544973	27.18959111648504	22.346029236230603	27.89006185183463
23	24.10325522461639	25.216027978995083	22.85860747863491	27.82210931775362
24	21.825385343512714	28.25769756992812	22.9934029686641	26.92351411789507
25	25.230289740351807	26.123443981258536	22.947439996527827	25.69882628186183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	149.0
2	294.5
3	440.0
4	440.0
5	440.0
6	1044.5
7	1649.0
8	1649.0
9	1649.0
10	1993.0
11	2337.0
12	2337.0
13	2337.0
14	3241.5
15	4146.0
16	4146.0
17	4146.0
18	6593.0
19	9040.0
20	9040.0
21	9040.0
22	12738.5
23	16437.0
24	16437.0
25	16437.0
26	23950.0
27	31463.0
28	31463.0
29	31463.0
30	40329.5
31	49196.0
32	49196.0
33	49196.0
34	54317.5
35	59439.0
36	59439.0
37	59439.0
38	64386.5
39	69334.0
40	69334.0
41	69334.0
42	77346.0
43	85358.0
44	85358.0
45	85358.0
46	119374.5
47	153391.0
48	153391.0
49	153391.0
50	182625.0
51	211859.0
52	211859.0
53	211859.0
54	152429.0
55	92999.0
56	92999.0
57	92999.0
58	77336.5
59	61674.0
60	61674.0
61	61674.0
62	54377.5
63	47081.0
64	47081.0
65	47081.0
66	39300.5
67	31520.0
68	31520.0
69	31520.0
70	24670.0
71	17820.0
72	17820.0
73	17820.0
74	14691.5
75	11563.0
76	11563.0
77	11563.0
78	9979.0
79	8395.0
80	8395.0
81	8395.0
82	6338.5
83	4282.0
84	4282.0
85	4282.0
86	3191.0
87	2100.0
88	2100.0
89	2100.0
90	1442.5
91	785.0
92	785.0
93	785.0
94	433.5
95	82.0
96	82.0
97	82.0
98	51.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16914140941577838
2	0.022209449503834727
3	0.03177185137354135
4	0.04462454205863089
5	0.032388780526425645
6	0.06827349291919566
7	0.07413431987159648
8	0.07742460868697941
9	0.05716876816727828
10	0.04626968646632235
11	0.6177517250881438
12	0.5495810536944288
13	0.5267546750377098
14	0.514004805878101
15	0.5267546750377098
16	0.5044424040083944
17	0.5094806587569495
18	0.5082468004511809
19	0.5235672077478076
20	0.5144160919800239
21	0.5200712758814632
22	0.5062931914670473
23	0.5116399107920445
24	0.498787220106955
25	0.500226721463685
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	972559.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.46312246471836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.40599873531319	44.10779618331128
2	8.160841212384376	8.23643059031347
3	1.7413569909350435	2.6362293326504567
4	0.7107738249009716	1.4347146628277607
5	0.37050263016672336	0.9348359799801809
6	0.26067247541977395	0.7892608230173603
7	0.17877080031964515	0.6314932952752176
8	0.1384551094681151	0.5589501715964383
9	0.10359575499453812	0.4704988744002902
>10	0.7154108725717826	7.124675547264463
>50	0.10200740403879986	3.67790385343217
>100	0.09158087343007681	9.173282336372472
>500	0.01185645236025044	4.1333154523109386
>1k	0.006541490957379554	6.823973008031869
>5k	6.132647772543331E-4	2.315267345803801
>10k+	0.0010221079620905552	6.951372543411822
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	20961	2.1552419956012954	No Hit
CTTCCGTACTCTGCGTTGATACCAC	13325	1.3700968270305451	No Hit
GTACATGGGAAGCAGTGGTATCAAC	12561	1.2915411815632778	No Hit
GAGTACGGAAGCAGTGGTATCAACG	10512	1.0808598758532901	No Hit
TCCGTACTCTGCGTTGATACCACTG	10027	1.0309914359951429	No Hit
CCCATGTACTCTGCGTTGATACCAC	9774	1.0049775900485214	No Hit
GCTTCCGTACTCTGCGTTGATACCA	7416	0.7625244329649924	No Hit
GCGTTGATACCACTGCTTCCGTACT	5254	0.5402242948756836	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4147	0.4264008661685307	No Hit
GTATCAACGCAGAGTACGGAAGCAG	4027	0.4140622831108447	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3697	0.3801311797022083	No Hit
GTACATGGAAGCAGTGGTATCAACG	3400	0.34959318663443556	No Hit
TATCAACGCAGAGTACGGAAGCAGT	3339	0.3433210735801118	No Hit
TCCATGTACTCTGCGTTGATACCAC	3012	0.30969843474791764	No Hit
CATGTACTCTGCGTTGATACCACTG	2871	0.29520059965513656	No Hit
GTATCAACGCAGAGTACATGGGAAG	2830	0.29098491711042723	No Hit
GCGTTGATACCACTGCTTCCCATGT	2790	0.2868720560911986	No Hit
GGTATCAACGCAGAGTACGGAAGCA	2376	0.24430394454218202	No Hit
ATACCACTGCTTCCGTACTCTGCGT	2319	0.23844311758978118	No Hit
TATCAACGCAGAGTACATGGGAAGC	2246	0.2309371462296889	No Hit
CAGTGGTATCAACGCAGAGTACGGA	2191	0.2252819623282495	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	1964	0.20194147604412688	No Hit
GTACTCTGCGTTGATACCACTGCTT	1949	0.20039915316191614	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1849	0.1901170006138445	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1829	0.18806057010423016	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1818	0.1869295333239423	No Hit
GTGGTATCAACGCAGAGTACGGAAG	1570	0.16142979500472465	No Hit
GGTATCAACGCAGAGTACATGGGAA	1527	0.15700846940905386	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1526	0.15690564788357314	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1454	0.14950249804896154	No Hit
CAGTGGTATCAACGCAGAGTACATG	1334	0.1371639149912756	No Hit
GTTGATACCACTGCTTCCGTACTCT	1237	0.1271902270196461	No Hit
GTACGGGAAGCAGTGGTATCAACGC	1217	0.1251337965100318	No Hit
GATACCACTGCTTCCGTACTCTGCG	1200	0.1233858305768596	No Hit
GATATACACTGTTCTACAAATCCCG	1167	0.11999272023599597	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1100	0.11310367802878796	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	1075	0.11053313989177008	No Hit
TTGTAGAACAGTGTATATCAATGAG	1044	0.10734567260186785	No Hit
ATACCACTGCTTCCCATGTACTCTG	1028	0.1057005281941764	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	1018	0.10467231293936922	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1000	0.10282152548071632	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0282152548071634E-4	0.0
10	0.0	0.0	0.0	2.0564305096143267E-4	0.0
11	0.0	0.0	0.0	2.0564305096143267E-4	0.0
12	0.0	0.0	0.0	4.1128610192286535E-4	0.0
13	0.0	0.0	0.0	6.16929152884298E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	70	1.2896635E-9	17.66341	10
AGAACCA	60	7.5748176E-8	17.436956	5
CGGTCCA	135	0.0	16.908564	10
CCGTCGT	85	1.6878403E-8	14.546338	9
CGCCGGT	150	0.0	13.949566	7
TTTTTCG	110	1.8371793E-10	13.826414	15
TCGTCAC	105	1.3551471E-9	13.579514	19
GTCGTAG	85	2.657507E-7	13.427389	11
TATACTG	100	9.889845E-9	13.315494	5
TCTATAC	50	0.001539248	13.253125	3
AGGCCCG	115	3.6925485E-10	13.232789	10
GATATAT	65	5.5696128E-5	13.123022	1
GTTTTCG	95	7.318158E-8	13.007745	15
GCCGGTC	170	0.0	12.867914	8
GGTCCAA	185	0.0	12.852794	11
GTCTAAA	60	4.171297E-4	12.636985	1
TATTGCG	105	2.0905645E-8	12.622024	2
TTTTCGG	100	1.429089E-7	12.357358	16
TAGACAG	70	1.0782953E-4	12.228516	5
CGAGGAT	140	5.0931703E-11	12.221562	16
>>END_MODULE