FastQCFastQC Report
Thu 2 Feb 2017
SRR4050272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972559
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA181941.870734834596153No Hit
CTTCCGTACTCTGCGTTGATACCAC127391.3098434130988454No Hit
TCCGTACTCTGCGTTGATACCACTG107611.1064624356979884No Hit
CCCATGTACTCTGCGTTGATACCAC104651.0760272641556965No Hit
GTACATGGGAAGCAGTGGTATCAAC103621.0654366470311827No Hit
GAGTACGGAAGCAGTGGTATCAACG97301.00045344292737No Hit
GCTTCCGTACTCTGCGTTGATACCA69940.71913374921213No Hit
GCGTTGATACCACTGCTTCCGTACT49200.5058819053651245No Hit
ACTCTGCGTTGATACCACTGCTTCC41920.4310278348151629No Hit
TATCAACGCAGAGTACGGAAGCAGT39450.4056309180214259No Hit
GTATCAACGCAGAGTACGGAAGCAG38700.3979193036103722No Hit
GAGTACATGGGAAGCAGTGGTATCA37680.3874315080113392No Hit
TCCATGTACTCTGCGTTGATACCAC34390.3536032261281835No Hit
CATGTACTCTGCGTTGATACCACTG34260.35226654629693416No Hit
GTACATGGAAGCAGTGGTATCAACG28930.29746267321571235No Hit
GCGTTGATACCACTGCTTCCCATGT28440.2924244184671573No Hit
GTATCAACGCAGAGTACATGGGAAG26190.2692895752339961No Hit
CAGTGGTATCAACGCAGAGTACGGA25700.264251320485441No Hit
ACGCAGAGTACGGAAGCAGTGGTAT25100.258082028956598No Hit
TATCAACGCAGAGTACATGGGAAGC24990.2569509921763101No Hit
GGTATCAACGCAGAGTACGGAAGCA24070.24749141183208423No Hit
ATACCACTGCTTCCGTACTCTGCGT23680.24348137233833628No Hit
GTATCAACGCAGAGTACTTTTTTTT22480.23114278928065032No Hit
AAGCAGTGGTATCAACGCAGAGTAC21110.2170562402897922No Hit
ACGCAGAGTACATGGGAAGCAGTGG20290.20862487520037346No Hit
GCTTCCCATGTACTCTGCGTTGATA18410.18929442840999877No Hit
GAAGCAGTGGTATCAACGCAGAGTA17430.17921791891288857No Hit
GTGGTATCAACGCAGAGTACGGAAG17360.17849816823452355No Hit
TATCAACGCAGAGTACTTTTTTTTT17270.17757277450519712No Hit
GTACTCTGCGTTGATACCACTGCTT15740.16184108110664752No Hit
GGTATCAACGCAGAGTACATGGGAA14350.14754888906482794No Hit
CAGTGGTATCAACGCAGAGTACATG14030.14425860024944503No Hit
GTTGATACCACTGCTTCCGTACTCT12890.13253694634464336No Hit
ATCAACGCAGAGTACGGAAGCAGTG12570.12924665752926043No Hit
GATACCACTGCTTCCGTACTCTGCG11910.12246043684753316No Hit
CCACTGCTTCCGTACTCTGCGTTGA11520.11845039735378522No Hit
TTGTAGAACAGTGTATATCAATGAG11180.11495446548744086No Hit
GATATACACTGTTCTACAAATCCCG11060.11372060718167226No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10920.11228110582494225No Hit
GGGAAGCAGTGGTATCAACGCAGAG10810.11115006904465435No Hit
CGGAAGCAGTGGTATCAACGCAGAG10690.10991621073888577No Hit
ATACCACTGCTTCCCATGTACTCTG10330.10621463582157999No Hit
GTACGGGAAGCAGTGGTATCAACGC10170.10456949141388852No Hit
AAAAAGTACTCTGCGTTGATACCAC10100.1038497407355235No Hit
GAGTACATGGAAGCAGTGGTATCAA9750.10025098734369843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTG250.00604966218.98956110
CGTCCGT456.611993E-414.8207221
CCCTATT500.001466416913.338651
CCAACGT500.001492857213.30706319
CTGGACC655.3795535E-513.171684
TCGCCAG1450.013.0942317
GCATCGC1501.8189894E-1212.65775514
CCGCATC1457.2759576E-1212.44143712
CGCATCG1405.2750693E-1112.20569213
ATCGCCA1405.2750693E-1112.20569216
TCATACT550.003036053412.1073024
TAGAGTT550.00306546912.0917275
GATATAC4750.012.0348731
TAATACT951.0215717E-612.0162714
CGCCAGT1459.822543E-1111.78480618
ATTACCG1406.85759E-1011.52759815
CGGTTCT1657.2759576E-1211.50882412
CCCTATA752.0463702E-411.4154572
TGTAGAA5650.011.2807612
ACTGTTC4950.011.125778
Produced by FastQC (version 0.11.5)