##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050272_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 972559 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.762105949356286 31.0 31.0 34.0 27.0 34.0 2 30.622161740316013 31.0 31.0 34.0 27.0 34.0 3 30.946423815933016 31.0 31.0 34.0 27.0 34.0 4 34.57213289887812 37.0 35.0 37.0 31.0 37.0 5 34.49217065494227 37.0 35.0 37.0 31.0 37.0 6 34.5269551770124 37.0 35.0 37.0 31.0 37.0 7 34.45400022003806 37.0 35.0 37.0 31.0 37.0 8 34.434781848710465 37.0 35.0 37.0 31.0 37.0 9 36.030272713532035 39.0 37.0 39.0 31.0 39.0 10 35.91793402765282 39.0 37.0 39.0 31.0 39.0 11 35.91857049289555 39.0 37.0 39.0 31.0 39.0 12 35.82894302556451 39.0 37.0 39.0 30.0 39.0 13 35.801427985345875 39.0 37.0 39.0 30.0 39.0 14 36.98012048626356 40.0 37.0 41.0 31.0 41.0 15 36.96829189797226 40.0 37.0 41.0 31.0 41.0 16 36.896231488269606 40.0 37.0 41.0 31.0 41.0 17 36.86046090777012 40.0 37.0 41.0 31.0 41.0 18 36.81098935900033 40.0 37.0 41.0 31.0 41.0 19 36.87994867149448 40.0 37.0 41.0 31.0 41.0 20 36.81927266109305 40.0 37.0 41.0 31.0 41.0 21 36.753330132156506 40.0 37.0 41.0 31.0 41.0 22 36.705322761909564 40.0 37.0 41.0 31.0 41.0 23 36.64069737671442 40.0 37.0 41.0 30.0 41.0 24 36.56064876269717 40.0 37.0 41.0 30.0 41.0 25 36.36524467924311 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5897689418680017 1101 2 0.9120293192801867 1101 3 0.6583342658012654 1101 4 0.645085587482157 1101 5 0.5377923752269425 1101 6 0.45115613580108516 1101 7 0.5641815344761696 1101 8 0.5349941847491095 1101 9 0.6137904822269249 1101 10 0.6071401220685004 1101 11 0.6281727129981576 1101 12 0.7075101531717394 1101 13 0.6799058783338054 1101 14 0.7868341918671646 1101 15 0.740418921283819 1101 16 0.6532993584719478 1101 17 0.7158288501679166 1101 18 0.5801173041384757 1101 19 0.6866735181985746 1101 20 0.8038728770566479 1101 21 0.5214690527675572 1101 22 0.8045427181297882 1101 23 0.8956730763473502 1101 24 0.9179277431551256 1101 25 0.851281920637895 1102 1 0.5030529977463445 1102 2 0.8519131161653952 1102 3 0.6493474331956328 1102 4 0.5398064822690642 1102 5 0.5648175067289856 1102 6 0.4682244710253016 1102 7 0.45600216454000275 1102 8 0.4804181427601364 1102 9 0.5831234030048265 1102 10 0.6416861391895523 1102 11 0.6728075441369228 1102 12 0.55000519677656 1102 13 0.7247222338649095 1102 14 0.8708144350456735 1102 15 0.8251889789269811 1102 16 0.7906120181939329 1102 17 0.9348123594103015 1102 18 0.796211550136114 1102 19 0.8150772724530526 1102 20 0.7211674301788875 1102 21 0.7094316019017626 1102 22 0.9080945912460763 1102 23 0.9849766598497212 1102 24 0.9938590297550931 1102 25 0.873850536629007 1103 1 0.4902064798288315 1103 2 0.7815953338800661 1103 3 0.6885631194701425 1103 4 0.5851073612413416 1103 5 0.6317546264044438 1103 6 0.6399621316067723 1103 7 0.7122563903682675 1103 8 0.6994850798798069 1103 9 0.6973897992184774 1103 10 0.611260175700707 1103 11 0.6133078822031877 1103 12 0.8285717958299728 1103 13 0.7544720648317806 1103 14 0.7896785324087574 1103 15 0.6737352940047359 1103 16 0.7865552230620878 1103 17 0.899653468267644 1103 18 0.8861371755586944 1103 19 0.6541577322231689 1103 20 0.7475365985358877 1103 21 0.6688636505833045 1103 22 0.903361663592257 1103 23 0.9112782149545211 1103 24 0.9059618018984352 1103 25 0.9083332952496974 1104 1 0.3770206190683645 1104 2 0.7098555304645728 1104 3 0.48531804810237134 1104 4 0.402054169262108 1104 5 0.4231196435904252 1104 6 0.4137239952705585 1104 7 0.3807731890471828 1104 8 0.38365505312174264 1104 9 0.4218058427535709 1104 10 0.39755625062148425 1104 11 0.5014227672857317 1104 12 0.5376519866060292 1104 13 0.6236461208564066 1104 14 0.6583200713830379 1104 15 0.639103904768966 1104 16 0.618350503082624 1104 17 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2212 1 -0.1301469953791532 2212 2 -0.9062963608400203 2212 3 -0.38571881982715794 2212 4 -0.14996647888538206 2212 5 -0.2092808749543238 2212 6 -0.3024580371790435 2212 7 -0.06936244610533038 2212 8 -0.04235846900692053 2212 9 -0.17251023125568565 2212 10 -0.1205549823269294 2212 11 -0.13902221801784975 2212 12 -0.17052403935298344 2212 13 -0.14664282026970454 2212 14 -0.21649259157667444 2212 15 -0.14833831599380431 2212 16 -0.10122815631783766 2212 17 -1.2186143917121441 2212 18 -0.4770327583832028 2212 19 -0.15159941254422193 2212 20 -0.2724363477796956 2212 21 -0.09329656976966305 2212 22 -0.21883849594651394 2212 23 -0.2129692051157619 2212 24 0.004990275335394756 2212 25 -0.06652656339543483 2213 1 -0.22802735806684993 2213 2 -0.5580051167649422 2213 3 -0.3568899821382594 2213 4 -0.16395630453550325 2213 5 -0.3647232140637584 2213 6 -0.16018811089750074 2213 7 -0.239899498812008 2213 8 -0.07295981779481053 2213 9 -0.39635046771154236 2213 10 -0.3142534519379723 2213 11 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-0.5318435551491376 2214 23 -0.5750676643933872 2214 24 -0.7157382957651564 2214 25 -0.6811328104774006 2215 1 -0.9317299779297663 2215 2 -2.188801434188708 2215 3 -1.3015378917078095 2215 4 -0.9221395177540401 2215 5 -0.834317025873311 2215 6 -0.7046468887651613 2215 7 -0.6848119843104357 2215 8 -0.8800426757600306 2215 9 -0.9497378814337338 2215 10 -0.9272887070813667 2215 11 -0.9679670673978436 2215 12 -0.7779763832767372 2215 13 -1.0041743907145033 2215 14 -1.0941252949810973 2215 15 -1.0978496370359636 2215 16 -1.0585904386282081 2215 17 -0.9667925890623579 2215 18 -1.0600996271963012 2215 19 -1.1067347982450002 2215 20 -1.0747578274133858 2215 21 -1.0316123076190848 2215 22 -0.9902665203833152 2215 23 -1.073157409855014 2215 24 -1.1858482874914387 2215 25 -1.0772968895805946 2216 1 -2.620697159826914 2216 2 -3.8932672908893053 2216 3 -2.807555564707208 2216 4 -2.285041531161909 2216 5 -2.539580506128992 2216 6 -2.336343864198909 2216 7 -2.3715811773882365 2216 8 -2.4389888660983488 2216 9 -2.634260365230702 2216 10 -2.6311225897959005 2216 11 -2.785471354798453 2216 12 -2.787362728184192 2216 13 -2.767255138615347 2216 14 -3.10956411321758 2216 15 -2.8957251465127882 2216 16 -2.97047182796873 2216 17 -2.8340864040693674 2216 18 -2.9162810009462703 2216 19 -3.0860383462082055 2216 20 -2.8061271229433586 2216 21 -2.975141116723968 2216 22 -2.8435928750905504 2216 23 -3.211445567720446 2216 24 -3.113470561929624 2216 25 -2.99570960478583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12899.0 3 0.0 4 205.0 5 1755.0 6 2667.0 7 1900.0 8 1381.0 9 1224.0 10 1086.0 11 1070.0 12 972.0 13 1006.0 14 960.0 15 943.0 16 988.0 17 1111.0 18 1231.0 19 1646.0 20 2081.0 21 2631.0 22 3248.0 23 4157.0 24 4978.0 25 5704.0 26 6679.0 27 7711.0 28 8922.0 29 10758.0 30 14052.0 31 18616.0 32 24567.0 33 33206.0 34 46134.0 35 64513.0 36 98496.0 37 176813.0 38 406249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.651053574738924 17.585429154050548 13.585655729263218 25.177861541947312 2 13.687741394132091 21.907790012298502 37.922478292523415 26.48199030104599 3 19.705231250751883 27.120205560793742 28.467990116794972 24.706573071659406 4 13.548729249907321 16.912200848539772 34.48922848786918 35.04984141368374 5 15.046054837866807 35.90065651546854 32.47097526802743 16.58231337863722 6 33.985159031684944 34.662909615766445 15.935471724899926 15.416459627648685 7 26.3345697222548 31.680563184411213 22.44539625133462 19.53947084199937 8 26.068914024077948 34.48097480104737 21.817220689839584 17.6328904850351 9 27.680789798436855 16.304915672562732 17.582887700534762 38.431406828465654 10 14.722402180060671 27.80348604041339 31.468558794796646 26.00555298472929 11 34.24212226949392 23.55991867485677 20.458806264545302 21.739152791104008 12 24.806353019576548 24.580825760910255 28.530380876769357 22.08244034274384 13 31.038915099190106 19.84429435790703 25.470037306833284 23.64675323606958 14 25.786273467377296 20.60851422207643 25.099463529353695 28.505748781192576 15 29.261987877167638 24.15579741811433 23.73761625426073 22.844598450457305 16 24.97578540626022 24.50722530574138 28.15751104822971 22.35947823976869 17 24.338883296540363 27.117737844182205 26.581317945749305 21.962060913528127 18 25.7452761220656 22.926218357960803 30.52925323810689 20.799252281866703 19 25.195695068371172 28.583047403807893 23.501813257601853 22.719444270219082 20 23.070168493633805 26.71148999700789 26.972451028677952 23.24589048068035 21 24.12676541508784 28.028236358681962 24.059002267340528 23.78599595888967 22 22.57020637744347 27.129841952102336 22.64434595728491 27.655605713169283 23 24.008020153205493 24.59225746748239 23.41319212379826 27.986530255513852 24 22.153124807205874 27.921319431591503 23.51759310671026 26.407962654492362 25 25.245288030067663 25.607030612234393 23.49864989927003 25.649031458427913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 254.0 1 254.0 2 357.5 3 461.0 4 461.0 5 461.0 6 999.5 7 1538.0 8 1538.0 9 1538.0 10 1734.5 11 1931.0 12 1931.0 13 1931.0 14 2573.0 15 3215.0 16 3215.0 17 3215.0 18 5523.5 19 7832.0 20 7832.0 21 7832.0 22 12337.0 23 16842.0 24 16842.0 25 16842.0 26 24877.5 27 32913.0 28 32913.0 29 32913.0 30 41431.0 31 49949.0 32 49949.0 33 49949.0 34 55004.0 35 60059.0 36 60059.0 37 60059.0 38 64666.5 39 69274.0 40 69274.0 41 69274.0 42 76972.0 43 84670.0 44 84670.0 45 84670.0 46 118658.5 47 152647.0 48 152647.0 49 152647.0 50 182390.5 51 212134.0 52 212134.0 53 212134.0 54 152737.0 55 93340.0 56 93340.0 57 93340.0 58 77863.0 59 62386.0 60 62386.0 61 62386.0 62 54610.0 63 46834.0 64 46834.0 65 46834.0 66 39119.0 67 31404.0 68 31404.0 69 31404.0 70 24594.0 71 17784.0 72 17784.0 73 17784.0 74 14652.5 75 11521.0 76 11521.0 77 11521.0 78 9969.5 79 8418.0 80 8418.0 81 8418.0 82 6381.5 83 4345.0 84 4345.0 85 4345.0 86 3129.0 87 1913.0 88 1913.0 89 1913.0 90 1353.0 91 793.0 92 793.0 93 793.0 94 443.5 95 94.0 96 94.0 97 94.0 98 51.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16235518873405108 2 0.008534186614899457 3 0.0 4 0.15207303618597945 5 0.04698943714468736 6 0.05346719324997249 7 0.038352429004307194 8 0.022312271029315444 9 0.0163486225514339 10 0.01120754627739808 11 0.017068373229798914 12 0.017582480857202494 13 0.008534186614899457 14 0.009459580344225902 15 0.0014395013567300287 16 5.141076274035816E-4 17 0.0 18 0.0 19 0.0 20 0.0 21 0.005552362375958682 22 0.007094685258169427 23 9.25393729326447E-4 24 0.0019536089841336104 25 0.11824475430282379 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 972559.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.986769847460415 #Duplication Level Percentage of deduplicated Percentage of total 1 87.23017283876881 43.60354573445729 2 8.276967900125975 8.274777789168297 3 1.7768070498081605 2.6645053518631694 4 0.7045747950145087 1.4087767247484737 5 0.41234721461206497 1.0305952657027329 6 0.24533430783560445 0.7358081748878619 7 0.17525473870486488 0.6132292801821826 8 0.1326627165392074 0.5305104543187392 9 0.09917593322580823 0.44617360937090195 >10 0.729965920297807 7.192152450225953 >50 0.1029814308270914 3.669677711564962 >100 0.09386354257262385 9.562226284693198 >500 0.010774622986548297 3.8227361591265026 >1k 0.007666558663505519 8.237505478694294 >5k 4.144085764057038E-4 1.7321837955248627 >10k+ 0.0010360214410142594 6.475595735470577 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACGGAAGCAGTGGTATCAACGCA 18194 1.870734834596153 No Hit CTTCCGTACTCTGCGTTGATACCAC 12739 1.3098434130988454 No Hit TCCGTACTCTGCGTTGATACCACTG 10761 1.1064624356979884 No Hit CCCATGTACTCTGCGTTGATACCAC 10465 1.0760272641556965 No Hit GTACATGGGAAGCAGTGGTATCAAC 10362 1.0654366470311827 No Hit GAGTACGGAAGCAGTGGTATCAACG 9730 1.00045344292737 No Hit GCTTCCGTACTCTGCGTTGATACCA 6994 0.71913374921213 No Hit GCGTTGATACCACTGCTTCCGTACT 4920 0.5058819053651245 No Hit ACTCTGCGTTGATACCACTGCTTCC 4192 0.4310278348151629 No Hit TATCAACGCAGAGTACGGAAGCAGT 3945 0.4056309180214259 No Hit GTATCAACGCAGAGTACGGAAGCAG 3870 0.3979193036103722 No Hit GAGTACATGGGAAGCAGTGGTATCA 3768 0.3874315080113392 No Hit TCCATGTACTCTGCGTTGATACCAC 3439 0.3536032261281835 No Hit CATGTACTCTGCGTTGATACCACTG 3426 0.35226654629693416 No Hit GTACATGGAAGCAGTGGTATCAACG 2893 0.29746267321571235 No Hit GCGTTGATACCACTGCTTCCCATGT 2844 0.2924244184671573 No Hit GTATCAACGCAGAGTACATGGGAAG 2619 0.2692895752339961 No Hit CAGTGGTATCAACGCAGAGTACGGA 2570 0.264251320485441 No Hit ACGCAGAGTACGGAAGCAGTGGTAT 2510 0.258082028956598 No Hit TATCAACGCAGAGTACATGGGAAGC 2499 0.2569509921763101 No Hit GGTATCAACGCAGAGTACGGAAGCA 2407 0.24749141183208423 No Hit ATACCACTGCTTCCGTACTCTGCGT 2368 0.24348137233833628 No Hit GTATCAACGCAGAGTACTTTTTTTT 2248 0.23114278928065032 No Hit AAGCAGTGGTATCAACGCAGAGTAC 2111 0.2170562402897922 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2029 0.20862487520037346 No Hit GCTTCCCATGTACTCTGCGTTGATA 1841 0.18929442840999877 No Hit GAAGCAGTGGTATCAACGCAGAGTA 1743 0.17921791891288857 No Hit GTGGTATCAACGCAGAGTACGGAAG 1736 0.17849816823452355 No Hit TATCAACGCAGAGTACTTTTTTTTT 1727 0.17757277450519712 No Hit GTACTCTGCGTTGATACCACTGCTT 1574 0.16184108110664752 No Hit GGTATCAACGCAGAGTACATGGGAA 1435 0.14754888906482794 No Hit CAGTGGTATCAACGCAGAGTACATG 1403 0.14425860024944503 No Hit GTTGATACCACTGCTTCCGTACTCT 1289 0.13253694634464336 No Hit ATCAACGCAGAGTACGGAAGCAGTG 1257 0.12924665752926043 No Hit GATACCACTGCTTCCGTACTCTGCG 1191 0.12246043684753316 No Hit CCACTGCTTCCGTACTCTGCGTTGA 1152 0.11845039735378522 No Hit TTGTAGAACAGTGTATATCAATGAG 1118 0.11495446548744086 No Hit GATATACACTGTTCTACAAATCCCG 1106 0.11372060718167226 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1092 0.11228110582494225 No Hit GGGAAGCAGTGGTATCAACGCAGAG 1081 0.11115006904465435 No Hit CGGAAGCAGTGGTATCAACGCAGAG 1069 0.10991621073888577 No Hit ATACCACTGCTTCCCATGTACTCTG 1033 0.10621463582157999 No Hit GTACGGGAAGCAGTGGTATCAACGC 1017 0.10456949141388852 No Hit AAAAAGTACTCTGCGTTGATACCAC 1010 0.1038497407355235 No Hit GAGTACATGGAAGCAGTGGTATCAA 975 0.10025098734369843 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 2.0564305096143267E-4 6 0.0 0.0 0.0 0.0 2.0564305096143267E-4 7 0.0 0.0 0.0 0.0 2.0564305096143267E-4 8 0.0 0.0 0.0 0.0 2.0564305096143267E-4 9 0.0 0.0 0.0 1.0282152548071634E-4 2.0564305096143267E-4 10 0.0 0.0 0.0 2.0564305096143267E-4 2.0564305096143267E-4 11 0.0 0.0 0.0 2.0564305096143267E-4 2.0564305096143267E-4 12 0.0 0.0 0.0 5.141076274035816E-4 2.0564305096143267E-4 13 0.0 0.0 0.0 7.197506783650143E-4 2.0564305096143267E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGGTG 25 0.006049662 18.989561 10 CGTCCGT 45 6.611993E-4 14.820722 1 CCCTATT 50 0.0014664169 13.33865 1 CCAACGT 50 0.0014928572 13.307063 19 CTGGACC 65 5.3795535E-5 13.17168 4 TCGCCAG 145 0.0 13.09423 17 GCATCGC 150 1.8189894E-12 12.657755 14 CCGCATC 145 7.2759576E-12 12.441437 12 CGCATCG 140 5.2750693E-11 12.205692 13 ATCGCCA 140 5.2750693E-11 12.205692 16 TCATACT 55 0.0030360534 12.107302 4 TAGAGTT 55 0.003065469 12.091727 5 GATATAC 475 0.0 12.034873 1 TAATACT 95 1.0215717E-6 12.016271 4 CGCCAGT 145 9.822543E-11 11.784806 18 ATTACCG 140 6.85759E-10 11.527598 15 CGGTTCT 165 7.2759576E-12 11.508824 12 CCCTATA 75 2.0463702E-4 11.415457 2 TGTAGAA 565 0.0 11.280761 2 ACTGTTC 495 0.0 11.12577 8 >>END_MODULE