FastQCFastQC Report
Thu 2 Feb 2017
SRR4050271_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050271_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9980187
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT220930.2213685976024297No Hit
GTACAAAGGGCAGGGACTTAATCAA145230.14551831543837807No Hit
TATCAACGCAGAGTACTTTTTTTTT117780.11801382078311759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61650.016.3362871
GTATCAA202600.013.35732651
CGCTTAC5750.012.23666418
CTCGACG6100.011.69137713
ACGCTTA5950.011.665544517
CGTCGTA13200.011.09560910
TCGACGC6550.010.74254214
CGGTTCT17150.010.47876212
ATACCGT14650.010.3864986
CCGTCGT14800.010.2816449
TACCGTC14800.010.0241997
TAGATCG3900.09.99778655
GACGCTT7150.09.97415916
CGACCAT12450.09.93066510
CGACGCT7300.09.89936515
AACCGCG7850.09.8130087
CGGTCCA12550.09.77575510
GCGTTAT6300.09.766021
GTCGTAG14500.09.70729111
GTATTAG12600.09.6908971
Produced by FastQC (version 0.11.5)