##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050271_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9980187
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.671111172566206	31.0	31.0	34.0	27.0	34.0
2	30.791499998947916	31.0	31.0	34.0	27.0	34.0
3	30.88761623404451	33.0	31.0	34.0	27.0	34.0
4	34.42456428922625	37.0	35.0	37.0	31.0	37.0
5	34.44826053860514	37.0	35.0	37.0	31.0	37.0
6	34.4863352760825	37.0	35.0	37.0	31.0	37.0
7	34.4159167558684	37.0	35.0	37.0	31.0	37.0
8	34.38550119351471	37.0	35.0	37.0	31.0	37.0
9	35.95703607557654	39.0	37.0	39.0	31.0	39.0
10	35.84335433794978	39.0	37.0	39.0	31.0	39.0
11	35.736287506436504	39.0	37.0	39.0	30.0	39.0
12	35.664438652301804	39.0	37.0	39.0	30.0	39.0
13	35.66603521557261	39.0	37.0	39.0	30.0	39.0
14	36.84778050751955	40.0	37.0	41.0	31.0	41.0
15	36.805628391532146	40.0	37.0	41.0	31.0	41.0
16	36.80952671528099	40.0	37.0	41.0	31.0	41.0
17	36.780687075302296	40.0	37.0	41.0	31.0	41.0
18	36.75545969228833	40.0	37.0	41.0	31.0	41.0
19	36.778798934328584	40.0	38.0	41.0	30.0	41.0
20	36.740431316567516	40.0	38.0	41.0	30.0	41.0
21	36.69674486059229	40.0	37.0	41.0	30.0	41.0
22	36.62281618570874	40.0	37.0	41.0	30.0	41.0
23	36.55112534464534	40.0	37.0	41.0	30.0	41.0
24	36.49170862229335	40.0	37.0	41.0	30.0	41.0
25	36.25944974778529	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6138983385173482
1101	2	0.6243050530304366
1101	3	0.6028664789038878
1101	4	0.6494774832504788
1101	5	0.6485802276476917
1101	6	0.6637970462522418
1101	7	0.6175762400457074
1101	8	0.6537276769405196
1101	9	0.7108604125396596
1101	10	0.7603860963793636
1101	11	0.6179109388121802
1101	12	0.6814473881108398
1101	13	0.5741866577492729
1101	14	0.7456501267377718
1101	15	0.7155620567348393
1101	16	0.7850181230291753
1101	17	0.8103439885882722
1101	18	0.8453032371546882
1101	19	0.8467743016827356
1101	20	0.8214439578595929
1101	21	0.8317637501590767
1101	22	0.8959230920320991
1101	23	0.8519719884921315
1101	24	0.908485255137883
1101	25	0.9507420685304453
1102	1	0.5414143507576235
1102	2	0.5496427632752372
1102	3	0.5534143216497611
1102	4	0.5436506156248342
1102	5	0.48913538714072047
1102	6	0.46031943469939307
1102	7	0.47145134886500983
1102	8	0.48713761416619406
1102	9	0.5651151225994653
1102	10	0.6278904224725039
1102	11	0.5269483549824159
1102	12	0.4870701998433091
1102	13	0.5241575788786434
1102	14	0.6387871112243886
1102	15	0.6114608117362863
1102	16	0.6188806602066705
1102	17	0.5645329417345337
1102	18	0.6562431763444323
1102	19	0.6856270430754563
1102	20	0.6808877361700425
1102	21	0.7205256265957445
1102	22	0.7577097234550152
1102	23	0.7179742446173947
1102	24	0.6782686330627925
1102	25	0.747655811869322
1103	1	0.57256415071965
1103	2	0.5668156863336371
1103	3	0.5297589568091645
1103	4	0.561463967520929
1103	5	0.5452526439214083
1103	6	0.5204477722161869
1103	7	0.5381347875404288
1103	8	0.5801986582138383
1103	9	0.6175122792245418
1103	10	0.6292638861829545
1103	11	0.5456558709370256
1103	12	0.5248846239689513
1103	13	0.5392449550766614
1103	14	0.6975251598695493
1103	15	0.706094240060267
1103	16	0.7154629563246289
1103	17	0.5998425912616412
1103	18	0.6533701423593214
1103	19	0.6268529232561946
1103	20	0.702266707922206
1103	21	0.6430159649933671
1103	22	0.6956887630828348
1103	23	0.7609207294669815
1103	24	0.8227314481156824
1103	25	0.7966264727166603
1104	1	0.44447035928710577
1104	2	0.48213576747688336
1104	3	0.47993347476828063
1104	4	0.4541358965317599
1104	5	0.5259600601602088
1104	6	0.44864175499255765
1104	7	0.46150630335214515
1104	8	0.4951102647791359
1104	9	0.5645537206445752
1104	10	0.5459640701995028
1104	11	0.4595752394670427
1104	12	0.4762459206313707
1104	13	0.4805512731006516
1104	14	0.5500986260953908
1104	15	0.4536413627382956
1104	16	0.5672259660991941
1104	17	0.5610345782739685
1104	18	0.5905418381844569
1104	19	0.6906206609645054
1104	20	0.6640294871279551
1104	21	0.6491286978660966
1104	22	0.6123046409566157
1104	23	0.5921864687943739
1104	24	0.6332957150702399
1104	25	0.714726166993735
1105	1	0.3373631665306185
1105	2	0.37812288095978985
1105	3	0.38063854645070805
1105	4	0.39568162082557734
1105	5	0.41764549571235676
1105	6	0.3683924400758798
1105	7	0.3757047562128406
1105	8	0.415715592254557
1105	9	0.4645310307789714
1105	10	0.44433138098474245
1105	11	0.44336067735450513
1105	12	0.37343415987430717
1105	13	0.4406608864318997
1105	14	0.550130173576008
1105	15	0.578137228332487
1105	16	0.4548576263934905
1105	17	0.5063629740051212
1105	18	0.5726863510715958
1105	19	0.5806220580079966
1105	20	0.5040318030971846
1105	21	0.5432812210219922
1105	22	0.5380144834527485
1105	23	0.5362464291824551
1105	24	0.5366000752968674
1105	25	0.5338875235823792
1106	1	0.3173161061248244
1106	2	0.33090496528416224
1106	3	0.3665940261922138
1106	4	0.37682281692620734
1106	5	0.39413433142711796
1106	6	0.3618103493853866
1106	7	0.38671033656294895
1106	8	0.3361523657047485
1106	9	0.3412504265233167
1106	10	0.3853850016452185
1106	11	0.306043714185229
1106	12	0.3322867502878921
1106	13	0.27478521839663017
1106	14	0.3568332015463085
1106	15	0.4116952634521738
1106	16	0.33578986490616813
1106	17	0.4215537273127339
1106	18	0.42645510804200626
1106	19	0.3976987256545712
1106	20	0.4068207885787345
1106	21	0.36437028283180695
1106	22	0.3597028133976963
1106	23	0.19422871876533065
1106	24	0.3567015600586956
1106	25	0.411774521248212
1107	1	0.3268922582046301
1107	2	0.3080243089496655
1107	3	0.23092967925748553
1107	4	0.28089067031282866
1107	5	0.29958820436763034
1107	6	0.24817849278652915
1107	7	0.26654097233002716
1107	8	0.28570142471585314
1107	9	0.3534390254047963
1107	10	0.3642140189152343
1107	11	0.28170420646278416
1107	12	0.2985535102817636
1107	13	0.30899852945695017
1107	14	0.4007771871817738
1107	15	0.38562358817537046
1107	16	0.38224780959848204
1107	17	0.40053944176007406
1107	18	0.4085050294546022
1107	19	0.47196400867915855
1107	20	0.4492608949017409
1107	21	0.4430203651335134
1107	22	0.42025076281400686
1107	23	0.38680377277749045
1107	24	0.43081460200279054
1107	25	0.4837794424868349
1108	1	0.13563936341756389
1108	2	0.09081777711903172
1108	3	0.13418147625554866
1108	4	0.13848633377414643
1108	5	0.15596706126813586
1108	6	0.10511504851503872
1108	7	0.16391645580041825
1108	8	0.12344047456879537
1108	9	0.08965045378325698
1108	10	0.029334146364533353
1108	11	0.03579289314843237
1108	12	0.024283479094833638
1108	13	0.0703288585225863
1108	14	-0.005523134616090886
1108	15	0.09816914347126016
1108	16	0.09437748590736561
1108	17	0.04456062565511587
1108	18	-0.02266620236143524
1108	19	0.11826214191106743
1108	20	0.10432399753577215
1108	21	0.1477946187508934
1108	22	0.1641232185908308
1108	23	0.07429011277343989
1108	24	0.08874889576544831
1108	25	0.0917435750184481
1109	1	0.01764073125200838
1109	2	0.03816609551237704
1109	3	0.09522465659697232
1109	4	0.12841765865121602
1109	5	0.10466665395667718
1109	6	0.05098651714789071
1109	7	0.06398978996005411
1109	8	0.08836498758934397
1109	9	0.11344292844025006
1109	10	-0.027703397720472367
1109	11	0.0503994939227681
1109	12	0.05794545462882894
1109	13	0.032906200409833275
1109	14	0.048959331596933
1109	15	0.01339692068565057
1109	16	0.032264613615502924
1109	17	8.83021086650615E-5
1109	18	0.03317688524313667
1109	19	0.04727684357053619
1109	20	0.022570408848295642
1109	21	0.046274482117688365
1109	22	0.0794460801032244
1109	23	0.08873270828539859
1109	24	0.04965682917109859
1109	25	0.05651491516500329
1110	1	0.001621164578494927
1110	2	-0.039853217265424234
1110	3	0.017582971932107228
1110	4	-0.006838224797284909
1110	5	-0.013733769648275995
1110	6	0.026353162600898372
1110	7	0.0500235631008934
1110	8	0.062465954564522974
1110	9	0.058160937162902826
1110	10	-0.06373946761528515
1110	11	-0.061870517485836274
1110	12	-0.055650619007607816
1110	13	-0.0778685824007539
1110	14	-0.0667416544204329
1110	15	-0.008518747519424608
1110	16	-0.0247494642335937
1110	17	-0.05269607361690021
1110	18	-0.048387529936483986
1110	19	-0.08004438127583313
1110	20	-0.047019065169983776
1110	21	-0.10576048366485935
1110	22	-0.07300196732182229
1110	23	-0.018134377309195315
1110	24	-0.031542026293756464
1110	25	-0.019945653327539503
1111	1	-0.04209479682468853
1111	2	-0.10290579528126287
1111	3	-0.09664171084502726
1111	4	-0.038720288104570955
1111	5	-0.052852380889035544
1111	6	-0.05132627215657237
1111	7	-0.094279113885122
1111	8	-0.1053529779354534
1111	9	-0.08964936513812205
1111	10	-0.0700488997134201
1111	11	-0.19249343125584062
1111	12	-0.20756323287636036
1111	13	-0.17264259003398763
1111	14	-0.1369519797070211
1111	15	-0.15029244073360104
1111	16	-0.21408656488181066
1111	17	-0.19274730221016512
1111	18	-0.12458642398460995
1111	19	-0.12665141851621087
1111	20	-0.18351364862432717
1111	21	-0.14148156931499045
1111	22	-0.1675719168270362
1111	23	-0.14205975306526852
1111	24	-0.20059742885707266
1111	25	-0.11910110922455885
1112	1	-0.15896774010470338
1112	2	-0.14803540739783472
1112	3	-0.12973210812920044
1112	4	-0.25037804067692804
1112	5	-0.14925426251215868
1112	6	-0.2309995196644934
1112	7	-0.22165914436703815
1112	8	-0.22879180607065308
1112	9	-0.20468349335598646
1112	10	-0.24930196738436905
1112	11	-0.22620739391019384
1112	12	-0.25071878745995946
1112	13	-0.28294042224055715
1112	14	-0.3144377523725055
1112	15	-0.2794536035642494
1112	16	-0.2445199740046604
1112	17	-0.2788837124384784
1112	18	-0.30109397941807714
1112	19	-0.40056951337269453
1112	20	-0.31747351241613586
1112	21	-0.33536464930411114
1112	22	-0.3339558782771448
1112	23	-0.27924505514322107
1112	24	-0.2980913574201338
1112	25	-0.2492006717069657
1113	1	-0.29862169204910316
1113	2	-0.32631132070364544
1113	3	-0.16320458536192461
1113	4	-0.16930736621978326
1113	5	-0.17960782549933896
1113	6	-0.19787193496201638
1113	7	-0.24923110006572813
1113	8	-0.23930969767819477
1113	9	-0.22859128504396153
1113	10	-0.3756875544986755
1113	11	-0.27925003973618345
1113	12	-0.4531891027541164
1113	13	-0.33834967034506036
1113	14	-0.33569815584226603
1113	15	-0.2980344380643558
1113	16	-0.2904884509965626
1113	17	-0.3525065049879146
1113	18	-0.2750884677575911
1113	19	-0.4017101533873557
1113	20	-0.3340232285853517
1113	21	-0.370050219589956
1113	22	-0.38641790718229174
1113	23	-0.3948379010779064
1113	24	-0.4053739684587825
1113	25	-0.36589691341889363
1114	1	-0.393315770879763
1114	2	-0.3940438683586187
1114	3	-0.4140146312801498
1114	4	-0.4010562852466535
1114	5	-0.4133165760994757
1114	6	-0.4387790619750831
1114	7	-0.4960758776862235
1114	8	-0.4838254268914568
1114	9	-0.5197074994936202
1114	10	-0.5438304332872619
1114	11	-0.5845353502842201
1114	12	-0.6598170807684198
1114	13	-0.5654773012729493
1114	14	-0.7424134271844807
1114	15	-0.7529904474883793
1114	16	-0.7068219583080335
1114	17	-0.6979272669395513
1114	18	-0.7088208768536433
1114	19	-0.5740310416695138
1114	20	-0.7015717183536907
1114	21	-0.7250247693097833
1114	22	-0.8101918246854183
1114	23	-0.7063103669575099
1114	24	-0.7041534373940053
1114	25	-0.7083665276642321
1115	1	-0.4089523871747076
1115	2	-0.43177146120945764
1115	3	-0.44839583509410375
1115	4	-0.3199541689003169
1115	5	-0.3740118384567097
1115	6	-0.36969534616303434
1115	7	-0.45815797399792046
1115	8	-0.4899761294978475
1115	9	-0.4477261443649354
1115	10	-0.5217600632962487
1115	11	-0.557665967666118
1115	12	-0.5727984026595578
1115	13	-0.5799988389353885
1115	14	-0.6157137345586037
1115	15	-0.5480801515567606
1115	16	-0.6199657550376187
1115	17	-0.5751686512480205
1115	18	-0.5063961496326854
1115	19	-0.5504784805951601
1115	20	-0.578961954033602
1115	21	-0.6071173288439979
1115	22	-0.825234452820709
1115	23	-0.7667892068321791
1115	24	-0.6029692096701851
1115	25	-0.6579580503547859
1116	1	-0.4939535454543673
1116	2	-0.60647630201683
1116	3	-0.5572543995577135
1116	4	-0.6109762793555262
1116	5	-0.5859423374111117
1116	6	-0.6351520442129726
1116	7	-0.5938628583429235
1116	8	-0.7044720488907359
1116	9	-0.6631739258891187
1116	10	-0.6584216262618057
1116	11	-0.76221713029868
1116	12	-0.8409828230748104
1116	13	-0.8574102387982805
1116	14	-0.8641891806725894
1116	15	-0.9822070490879682
1116	16	-0.906445523916318
1116	17	-0.9200393353059511
1116	18	-0.8088611651919209
1116	19	-0.8352564737254369
1116	20	-0.8382635675626631
1116	21	-0.8964930394159296
1116	22	-0.9474216708249443
1116	23	-0.8104438357217703
1116	24	-0.905989903595902
1116	25	-0.8830282382145498
1201	1	0.48895397956276554
1201	2	0.5574601220588598
1201	3	0.5591145708594603
1201	4	0.5997840228971114
1201	5	0.5816964991340825
1201	6	0.6429783425575337
1201	7	0.6061565845779668
1201	8	0.6132751176067117
1201	9	0.7029222109419138
1201	10	0.6747931507159848
1201	11	0.614207088886495
1201	12	0.6131744643042438
1201	13	0.6464260689847521
1201	14	0.737857734867525
1201	15	0.8189439770584954
1201	16	0.7168437408256381
1201	17	0.6921175926573042
1201	18	0.7066877884125518
1201	19	0.6946127104245363
1201	20	0.732965856668855
1201	21	0.7195826303761734
1201	22	0.7147228163410944
1201	23	0.7588414557690513
1201	24	0.7482652423173874
1201	25	0.6704553429259974
1202	1	0.42269959403407853
1202	2	0.39817321776029146
1202	3	0.44033804959508416
1202	4	0.5195586102378158
1202	5	0.5554106844462936
1202	6	0.49457651649101564
1202	7	0.49049895664176546
1202	8	0.44398244721488567
1202	9	0.5375768712712272
1202	10	0.6048888390293072
1202	11	0.5032280174922761
1202	12	0.49915403881562526
1202	13	0.4209212076313804
1202	14	0.547827445914848
1202	15	0.5742481721875379
1202	16	0.5322357508091784
1202	17	0.5158917848573381
1202	18	0.5783996819358492
1202	19	0.5130238901874691
1202	20	0.4386522038129286
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2210	14	-0.10987988773381119
2210	15	-0.06404652449760562
2210	16	0.034397702676770336
2210	17	-0.06834693231199651
2210	18	-0.0338668813611136
2210	19	-0.0019051323374412732
2210	20	-0.05101340694333345
2210	21	-0.08622486336192026
2210	22	-0.0381154636202794
2210	23	-0.07677483759793091
2210	24	0.04247631805695562
2210	25	-0.021710267228222335
2211	1	-0.10355372110531391
2211	2	-0.12404540674545217
2211	3	-0.1038001834865021
2211	4	-0.14003065827648697
2211	5	-0.1355330169897897
2211	6	-0.137244533524715
2211	7	-0.08063047314346505
2211	8	-0.08770319294844597
2211	9	-0.06590129374702514
2211	10	-0.09875098827648543
2211	11	0.049726147686861566
2211	12	-0.022941711635596107
2211	13	0.07689318882697194
2211	14	-0.008135159658756663
2211	15	0.052653034346604954
2211	16	-0.022556956781066617
2211	17	-0.16352351688427547
2211	18	-0.0798235279374282
2211	19	-0.12137654828021738
2211	20	-0.10758947529005525
2211	21	-0.07188685090230962
2211	22	-0.08813603632717104
2211	23	-0.0036947953890518193
2211	24	-0.16687497638400117
2211	25	-0.15463781951966382
2212	1	-0.33938404256201693
2212	2	-0.2604777882472966
2212	3	-0.3843362101850367
2212	4	-0.3112778338154598
2212	5	-0.2895212174315489
2212	6	-0.2916679254764247
2212	7	-0.3340180505059678
2212	8	-0.3798141967738502
2212	9	-0.33827332463368975
2212	10	-0.3598394168875174
2212	11	-0.20081521468678432
2212	12	-0.22099557102269785
2212	13	-0.24515395104425863
2212	14	-0.22553302002662434
2212	15	-0.3347224441349539
2212	16	-0.26991035417085385
2212	17	-0.2670171156040553
2212	18	-0.37972288687480216
2212	19	-0.3616059723172498
2212	20	-0.362572352040452
2212	21	-0.34524782030444356
2212	22	-0.3059119736524707
2212	23	-0.29347779827948983
2212	24	-0.43149955727120926
2212	25	-0.46552019535614875
2213	1	-0.4040866053494767
2213	2	-0.3933960123557334
2213	3	-0.4630014115753909
2213	4	-0.40005098824720164
2213	5	-0.39681572515613084
2213	6	-0.32430130025599624
2213	7	-0.31090095036277177
2213	8	-0.32932898168687075
2213	9	-0.28321737597719476
2213	10	-0.3099328406063364
2213	11	-0.2735514172898874
2213	12	-0.3668570813341603
2213	13	-0.2823288690716197
2213	14	-0.45431960155437423
2213	15	-0.42481771674992785
2213	16	-0.35995949854451936
2213	17	-0.4294192520372988
2213	18	-0.3266404186566021
2213	19	-0.3971353320972639
2213	20	-0.3336620502531247
2213	21	-0.3677669595320978
2213	22	-0.3903678380705884
2213	23	-0.3563286061534683
2213	24	-0.4087758447359562
2213	25	-0.338345741436072
2214	1	-0.4142671671244429
2214	2	-0.45808516277414313
2214	3	-0.4944719698383011
2214	4	-0.6255520045746508
2214	5	-0.5471805624858419
2214	6	-0.5025817871809437
2214	7	-0.549257231585635
2214	8	-0.5423581094790393
2214	9	-0.5244962431096098
2214	10	-0.5332766505234687
2214	11	-0.48755062557878404
2214	12	-0.4669758919086675
2214	13	-0.5557723247519917
2214	14	-0.6596071028450439
2214	15	-0.5028184749063556
2214	16	-0.5924967647129762
2214	17	-0.600195191606339
2214	18	-0.6696026832235447
2214	19	-0.6145366426986953
2214	20	-0.6487632880967951
2214	21	-0.6538610046078617
2214	22	-0.6579674732979157
2214	23	-0.6000977604697937
2214	24	-0.6974964646308095
2214	25	-0.599232485773797
2215	1	-0.5957496919001422
2215	2	-0.5496796886582338
2215	3	-0.7055143961467856
2215	4	-0.6149641749947037
2215	5	-0.6976117852441988
2215	6	-0.6784193845722797
2215	7	-0.6408573267718509
2215	8	-0.7041760391241993
2215	9	-0.8131493616183505
2215	10	-0.7994161583173351
2215	11	-0.6941135253991035
2215	12	-0.7400774181856846
2215	13	-0.7342951074235131
2215	14	-0.8556698407403545
2215	15	-0.844070769617602
2215	16	-0.8491541448126227
2215	17	-0.8393273458207062
2215	18	-0.8817747984996984
2215	19	-0.973321637884915
2215	20	-0.976768851145664
2215	21	-0.8912102004861353
2215	22	-0.9655049228242802
2215	23	-0.874257942685631
2215	24	-0.9756950259823682
2215	25	-0.8472058582563946
2216	1	-0.5782025874934646
2216	2	-0.7208869284770678
2216	3	-0.7145915637356239
2216	4	-0.641001046767542
2216	5	-0.6408853790704185
2216	6	-0.5977378651164358
2216	7	-0.6568430414026452
2216	8	-0.6392973867165566
2216	9	-0.6998082999650137
2216	10	-0.8031681736943099
2216	11	-0.6208491982770283
2216	12	-0.6664624519881386
2216	13	-0.670414765944777
2216	14	-0.7122162214620147
2216	15	-0.6853290975615494
2216	16	-0.8983133413818578
2216	17	-0.9031259778768614
2216	18	-0.8221776190985679
2216	19	-0.8112594953281871
2216	20	-0.7522167509062356
2216	21	-0.8199554240775768
2216	22	-0.7762775290230124
2216	23	-0.9695193114397114
2216	24	-1.0753026647568475
2216	25	-1.0023386541495611
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	238181.0
3	11.0
4	1996.0
5	11106.0
6	15777.0
7	16549.0
8	14047.0
9	13507.0
10	13591.0
11	15922.0
12	26997.0
13	23367.0
14	10386.0
15	10570.0
16	10936.0
17	11690.0
18	12888.0
19	15402.0
20	19252.0
21	24679.0
22	31079.0
23	37963.0
24	45060.0
25	52092.0
26	60462.0
27	68084.0
28	78836.0
29	95895.0
30	122405.0
31	158215.0
32	206299.0
33	275350.0
34	382630.0
35	544343.0
36	826231.0
37	1557820.0
38	4930569.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.531723689289784	17.684276394196388	13.046348970365724	25.73765094614811
2	16.809185175104417	20.498959802062814	37.36328364730962	25.328571375523147
3	18.28514295310104	24.08810366454866	28.865245301135552	28.76150808121475
4	13.19164294423526	16.714874529211595	36.82385297798294	33.26962954857021
5	15.5904354455011	35.168367294697426	33.06522416949703	16.175973090304442
6	32.4673530298532	36.530185130762845	16.477759873552117	14.52470196583184
7	29.151197511140197	30.817284711060637	21.25871004687064	18.772807730928527
8	26.958637084168437	32.15594324666311	19.894346692872627	20.99107297629583
9	27.63107709213999	14.814498792803343	18.81770929421107	38.7367148208456
10	16.479079560836215	26.14454303905349	30.919767383566448	26.45661001654384
11	35.70007990371995	22.436627510009156	22.511909367908896	19.351383218361992
12	24.912001870568634	24.114437967459327	28.16608922543792	22.80747093653412
13	29.213883452280815	20.754730874535078	24.60113262789624	25.430253045287866
14	23.926891401786108	20.301500593368097	25.183893674892786	30.58771432995301
15	24.963991120415567	26.91034179863534	22.851887123523245	25.273779957425845
16	25.08326267627905	26.5141550332622	23.531638808200018	24.87094348225873
17	24.767310905594396	25.39204832876309	24.686574676724735	25.15406608891778
18	26.152189071469557	24.446517879696906	25.303761963445666	24.09753108538787
19	26.9681544853967	24.78216352589535	24.719055559422465	23.530626429285487
20	26.00593368592988	24.7563570126255	24.75417795832095	24.483531343123676
21	26.069989928236797	24.72739413679139	24.739135280877118	24.4634806540947
22	25.41411875760388	24.560960732317007	25.189728250941318	24.8351922591378
23	24.52149635565073	24.231533878308145	25.347854116297242	25.899115649743887
24	24.657232058596755	25.423946106090824	25.054831573464426	24.863990261848
25	24.82245232590403	24.707370226778302	25.23517445528395	25.235002992033717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4308.0
1	4308.0
2	5403.0
3	6498.0
4	6498.0
5	6498.0
6	11691.5
7	16885.0
8	16885.0
9	16885.0
10	23439.5
11	29994.0
12	29994.0
13	29994.0
14	40903.5
15	51813.0
16	51813.0
17	51813.0
18	84033.5
19	116254.0
20	116254.0
21	116254.0
22	158548.5
23	200843.0
24	200843.0
25	200843.0
26	271321.0
27	341799.0
28	341799.0
29	341799.0
30	410166.5
31	478534.0
32	478534.0
33	478534.0
34	572357.0
35	666180.0
36	666180.0
37	666180.0
38	745111.0
39	824042.0
40	824042.0
41	824042.0
42	921862.5
43	1019683.0
44	1019683.0
45	1019683.0
46	1091654.0
47	1163625.0
48	1163625.0
49	1163625.0
50	1194895.0
51	1226165.0
52	1226165.0
53	1226165.0
54	1157052.0
55	1087939.0
56	1087939.0
57	1087939.0
58	1004754.0
59	921569.0
60	921569.0
61	921569.0
62	808344.0
63	695119.0
64	695119.0
65	695119.0
66	584395.0
67	473671.0
68	473671.0
69	473671.0
70	373543.0
71	273415.0
72	273415.0
73	273415.0
74	221991.0
75	170567.0
76	170567.0
77	170567.0
78	140126.5
79	109686.0
80	109686.0
81	109686.0
82	84400.5
83	59115.0
84	59115.0
85	59115.0
86	44311.0
87	29507.0
88	29507.0
89	29507.0
90	20622.0
91	11737.0
92	11737.0
93	11737.0
94	6383.0
95	1029.0
96	1029.0
97	1029.0
98	619.5
99	210.0
100	210.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1449271441507058
2	0.1625019651435389
3	0.17548769376766188
4	0.19540716020651716
5	0.18609871738876235
6	0.06763400325064049
7	0.05509916798152179
8	0.05877645378788995
9	0.06680235550696595
10	0.06700275255363451
11	0.7090147709657144
12	0.6675626418623218
13	0.6655987508049699
14	0.6657390287376379
15	0.6679233565463252
16	0.6741557046977176
17	0.6655386316909693
18	0.676249853835404
19	0.671500443829359
20	0.6776426133097506
21	0.6646368449809608
22	0.6679834756603258
23	0.6524026052818449
24	0.6632240458019474
25	0.6565608440002176
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	9980187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.283154910925234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27533552666122	34.31286628216053
2	12.366065984318189	10.704846992759347
3	3.3103057828615627	4.29841434006386
4	1.3934126687305957	2.412451855820485
5	0.785248097746474	1.6994007529134998
6	0.5050709098934474	1.311663746035203
7	0.3412269069003181	1.0338563949799633
8	0.24651099976786225	0.853581903215955
9	0.18260134536130015	0.7113206086394863
>10	1.2876798064732524	11.067907557085746
>50	0.1555962204372761	4.721152538567573
>100	0.12393784526037001	10.695704906919493
>500	0.014982687506630513	4.5038063386933525
>1k	0.011327429861148961	9.446922112577134
>5k	6.28009398402379E-4	1.738897467517401
>10k+	6.977882204470879E-5	0.4872062020508852
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	22093	0.2213685976024297	No Hit
GTACAAAGGGCAGGGACTTAATCAA	14523	0.14551831543837807	No Hit
TATCAACGCAGAGTACTTTTTTTTT	11778	0.11801382078311759	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0039704666856444E-5
2	0.0	0.0	0.0	0.0	2.0039704666856444E-5
3	0.0	0.0	0.0	0.0	2.0039704666856444E-5
4	0.0	0.0	0.0	1.0019852333428222E-5	2.0039704666856444E-5
5	0.0	0.0	0.0	1.0019852333428222E-5	2.0039704666856444E-5
6	0.0	0.0	0.0	1.0019852333428222E-5	2.0039704666856444E-5
7	2.0039704666856444E-5	0.0	0.0	2.0039704666856444E-5	4.007940933371289E-5
8	2.0039704666856444E-5	0.0	0.0	3.0059557000284662E-5	4.007940933371289E-5
9	2.0039704666856444E-5	0.0	0.0	4.007940933371289E-5	4.007940933371289E-5
10	3.0059557000284662E-5	0.0	0.0	4.007940933371289E-5	4.007940933371289E-5
11	3.0059557000284662E-5	0.0	0.0	4.007940933371289E-5	4.007940933371289E-5
12	4.007940933371289E-5	0.0	0.0	5.009926166714111E-5	1.1021837566771043E-4
13	4.007940933371289E-5	0.0	0.0	6.0119114000569325E-5	2.0039704666856444E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6165	0.0	16.336287	1
GTATCAA	20260	0.0	13.3573265	1
CGCTTAC	575	0.0	12.236664	18
CTCGACG	610	0.0	11.691377	13
ACGCTTA	595	0.0	11.6655445	17
CGTCGTA	1320	0.0	11.095609	10
TCGACGC	655	0.0	10.742542	14
CGGTTCT	1715	0.0	10.478762	12
ATACCGT	1465	0.0	10.386498	6
CCGTCGT	1480	0.0	10.281644	9
TACCGTC	1480	0.0	10.024199	7
TAGATCG	390	0.0	9.9977865	5
GACGCTT	715	0.0	9.974159	16
CGACCAT	1245	0.0	9.930665	10
CGACGCT	730	0.0	9.899365	15
AACCGCG	785	0.0	9.813008	7
CGGTCCA	1255	0.0	9.775755	10
GCGTTAT	630	0.0	9.76602	1
GTCGTAG	1450	0.0	9.707291	11
GTATTAG	1260	0.0	9.690897	1
>>END_MODULE