FastQCFastQC Report
Thu 2 Feb 2017
SRR4050271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9980187
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT357380.35808948269205776No Hit
TATCAACGCAGAGTACTTTTTTTTT210560.21097801073266462No Hit
GTACAAAGGGCAGGGACTTAATCAA140830.14110958041166966No Hit
GGTATCAACGCAGAGTACTTTTTTT136440.13671086523729467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63900.016.3277341
GTATCAA215250.013.617041
AACCGCG7350.011.3694417
CGTCGTA13200.011.14934410
AGAACCG9900.010.8453615
TAGATCG4500.010.76857955
ACGGTAT12850.010.1970399
CCGTCGT14500.010.14999
CGCGCTA7900.010.09227816
CCGACCA14500.09.8879679
GCGTTAT6550.09.8828871
ATACCGT15600.09.8648616
GCGCGCT8450.09.77236315
GTCGTAG14500.09.75685411
ACACCGT5600.09.669136
CGACCAT13650.09.66882510
CGAGCCG17700.09.65242115
TACCGTC15550.09.6487377
GAGCGTT5700.09.4960677
CTCGACG4800.09.49159513
Produced by FastQC (version 0.11.5)