##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9980187
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303393012575818	31.0	31.0	34.0	28.0	34.0
2	31.38430332016825	33.0	31.0	34.0	28.0	34.0
3	31.471124739446264	33.0	31.0	34.0	28.0	34.0
4	34.99429469608135	37.0	35.0	37.0	32.0	37.0
5	34.92279894154288	37.0	35.0	37.0	32.0	37.0
6	34.940924654017	37.0	35.0	37.0	32.0	37.0
7	34.863913070967506	37.0	35.0	37.0	32.0	37.0
8	34.8411786272141	37.0	35.0	37.0	32.0	37.0
9	36.48364785148815	39.0	37.0	39.0	32.0	39.0
10	36.37430992024498	39.0	37.0	39.0	32.0	39.0
11	36.39905855471446	39.0	37.0	39.0	32.0	39.0
12	36.29116187903092	39.0	37.0	39.0	32.0	39.0
13	36.31697351963445	39.0	37.0	39.0	32.0	39.0
14	37.532191030087915	40.0	38.0	41.0	32.0	41.0
15	37.51209431246128	40.0	38.0	41.0	32.0	41.0
16	37.488499063193906	40.0	38.0	41.0	32.0	41.0
17	37.467499657070555	40.0	38.0	41.0	32.0	41.0
18	37.42887743486169	40.0	38.0	41.0	32.0	41.0
19	37.444286164177086	40.0	38.0	41.0	32.0	41.0
20	37.404608350524896	40.0	38.0	41.0	32.0	41.0
21	37.36537421593403	40.0	38.0	41.0	32.0	41.0
22	37.32289505196646	40.0	38.0	41.0	32.0	41.0
23	37.257404495526984	40.0	38.0	41.0	32.0	41.0
24	37.19780330769353	40.0	38.0	41.0	32.0	41.0
25	36.9028806774863	40.0	37.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.46823738444297547
1101	2	0.4304235936769416
1101	3	0.4565565174153896
1101	4	0.369232326410291
1101	5	0.4471460744998268
1101	6	0.4804859725243702
1101	7	0.513772571196661
1101	8	0.5022967947325938
1101	9	0.5612032372931992
1101	10	0.6268865927887859
1101	11	0.5863742902539428
1101	12	0.6275016989702564
1101	13	0.6139106832966021
1101	14	0.6497162863919641
1101	15	0.6822625790874
1101	16	0.6979957217707593
1101	17	0.6802811751209745
1101	18	0.7338355616624312
1101	19	0.7138540687922159
1101	20	0.7424018654445632
1101	21	0.6784340810456939
1101	22	0.6816071632141245
1101	23	0.7064783147472795
1101	24	0.7515877584687374
1101	25	0.8368267117799917
1102	1	0.42409349473590296
1102	2	0.37422125199026013
1102	3	0.4127352823101553
1102	4	0.31260555779724797
1102	5	0.33822909850979244
1102	6	0.38200538508402815
1102	7	0.39721775696713735
1102	8	0.4445396455171675
1102	9	0.5002304221284462
1102	10	0.505730258853859
1102	11	0.483533361280692
1102	12	0.5826917028472778
1102	13	0.502846298353802
1102	14	0.5958542068524082
1102	15	0.6564635479595324
1102	16	0.4587908870492825
1102	17	0.558953236885614
1102	18	0.6197083677985162
1102	19	0.6686977817130568
1102	20	0.6590628645813581
1102	21	0.5638523961230604
1102	22	0.6211858017670693
1102	23	0.6512725516461941
1102	24	0.6876927265157278
1102	25	0.8639575735474736
1103	1	0.3964505183745537
1103	2	0.36182741400984497
1103	3	0.3821822102215151
1103	4	0.32692817195182045
1103	5	0.3409955087135472
1103	6	0.434304349836502
1103	7	0.4290564221297828
1103	8	0.4783967743372344
1103	9	0.476100584843266
1103	10	0.48335085683274315
1103	11	0.4949974858887458
1103	12	0.4994340307948377
1103	13	0.5014849678942781
1103	14	0.5826541527625579
1103	15	0.6173710639093102
1103	16	0.5992618915391859
1103	17	0.5292101885534564
1103	18	0.619824003333342
1103	19	0.6302841943661761
1103	20	0.6162116047321788
1103	21	0.6291858161431776
1103	22	0.7419389253604152
1103	23	0.7414612671864731
1103	24	0.7563944645592287
1103	25	0.7982992037098953
1104	1	0.3535354665736641
1104	2	0.346242772835037
1104	3	0.3676593172137892
1104	4	0.27635164382871835
1104	5	0.3573146459391978
1104	6	0.37319252438642536
1104	7	0.3819981902078311
1104	8	0.32914641295951697
1104	9	0.35404005784217674
1104	10	0.4085013878553525
1104	11	0.36791251081351817
1104	12	0.3794101478264764
1104	13	0.401946374281998
1104	14	0.4582548234207522
1104	15	0.43677028928296835
1104	16	0.4497570274566769
1104	17	0.5001265072097425
1104	18	0.5348876014261563
1104	19	0.5403499234523466
1104	20	0.5804158530545109
1104	21	0.6041259566600203
1104	22	0.5748204145463802
1104	23	0.5822981584229225
1104	24	0.6120657713171695
1104	25	0.7406407615601012
1105	1	0.30185212589583443
1105	2	0.25978545817924825
1105	3	0.28182922119365017
1105	4	0.2908083217311557
1105	5	0.2519152593946714
1105	6	0.3583740246563707
1105	7	0.31624308405532275
1105	8	0.336399818546667
1105	9	0.3888520325085665
1105	10	0.4492319213808713
1105	11	0.43132800439892804
1105	12	0.4847046939262185
1105	13	0.4774441798458753
1105	14	0.4983869911969663
1105	15	0.5185776324254547
1105	16	0.45412271236519075
1105	17	0.48095800649166875
1105	18	0.48357675040557524
1105	19	0.524948493161709
1105	20	0.5666230817251048
1105	21	0.5849235869647273
1105	22	0.5667427947547381
1105	23	0.579996988734095
1105	24	0.5579984747234548
1105	25	0.5540775009849597
1106	1	0.28107274434815466
1106	2	0.23826021661973584
1106	3	0.27646162281373066
1106	4	0.2533672355058556
1106	5	0.20561240565618277
1106	6	0.29537915655348
1106	7	0.2830418911790389
1106	8	0.33763290134164947
1106	9	0.35091605102115864
1106	10	0.35514811422783055
1106	11	0.35672358926616
1106	12	0.435379331605958
1106	13	0.393946913475439
1106	14	0.4069975821185352
1106	15	0.38561365358705046
1106	16	0.3583325594718332
1106	17	0.37202165161933465
1106	18	0.46952730074909255
1106	19	0.5364075645467778
1106	20	0.5188377149376961
1106	21	0.5012085036695737
1106	22	0.5243058519396655
1106	23	0.5037806061419801
1106	24	0.5849165319963774
1106	25	0.6642809419926436
1107	1	0.21082648709592178
1107	2	0.14270211530762467
1107	3	0.14726999666819296
1107	4	0.11729366145149811
1107	5	0.18030996742943017
1107	6	0.245534631639579
1107	7	0.1932631473265829
1107	8	0.2211919960164721
1107	9	0.23038251427634293
1107	10	0.20837297015448542
1107	11	0.20690139553449427
1107	12	0.23698086614695768
1107	13	0.20075302235191828
1107	14	0.2695574138904746
1107	15	0.136509462465213
1107	16	0.13189455834772446
1107	17	0.16792118333959394
1107	18	0.2507464883071151
1107	19	0.19613782286377557
1107	20	0.17083899338386743
1107	21	0.04410897549284698
1107	22	-0.03463511422324217
1107	23	0.15121690690492784
1107	24	0.2081319185838595
1107	25	0.38381322046379296
1108	1	0.17808655567668552
1108	2	0.13556567131162822
1108	3	0.11291284723007777
1108	4	0.08854698638152314
1108	5	0.15114982921992492
1108	6	0.12943591327370285
1108	7	0.10976440693376333
1108	8	0.1202728802996802
1108	9	0.14882402646918536
1108	10	0.17015283261967795
1108	11	0.17543652574205026
1108	12	0.11893846765729421
1108	13	0.07450122524920033
1108	14	0.1038177436618497
1108	15	0.11482780690394634
1108	16	0.09602531514221369
1108	17	0.08836857718786462
1108	18	0.09615891952218192
1108	19	0.11376884438979573
1108	20	0.12553532501802067
1108	21	0.16385038631777604
1108	22	0.20930078153411102
1108	23	0.07551751644155758
1108	24	0.10908209756414777
1108	25	0.26301255209211405
1109	1	-0.05821305947902644
1109	2	0.0076135936612438115
1109	3	-0.045535451120361614
1109	4	-0.03481288416930539
1109	5	0.019914453797852616
1109	6	0.01973776729374066
1109	7	0.031031304691381933
1109	8	0.03839789886583134
1109	9	0.058192224215844135
1109	10	0.024502535919786794
1109	11	0.01546470190245941
1109	12	-0.04689966214493779
1109	13	-0.036402166918769296
1109	14	-0.03275439697174676
1109	15	0.01691754449210947
1109	16	-0.018227521509473377
1109	17	-0.037572261411426666
1109	18	-0.08066588123528362
1109	19	-0.014669470066671408
1109	20	-0.017948553758934338
1109	21	0.0275839426839255
1109	22	0.08743995476252309
1109	23	0.08922321931418509
1109	24	0.08519229359878722
1109	25	0.2264561862841461
1110	1	0.013350453702670961
1110	2	-0.06414602055090413
1110	3	-0.040521386634193135
1110	4	0.03948435087298208
1110	5	0.07005706134022915
1110	6	0.07809423520349412
1110	7	0.11006173208806302
1110	8	0.08723682709184999
1110	9	0.05092600453926366
1110	10	0.09859790716710393
1110	11	0.06873076957569424
1110	12	0.12894884229238812
1110	13	0.13900865972892262
1110	14	0.03567266897933763
1110	15	0.025478423505795433
1110	16	0.12827823874950184
1110	17	0.12250727309083231
1110	18	0.14667998151738004
1110	19	0.07278653665488832
1110	20	0.12639136749998414
1110	21	0.08463923682712249
1110	22	0.08538356652925216
1110	23	0.16114824157181573
1110	24	0.07587711881998871
1110	25	0.26134220882407533
1111	1	-0.10713606978415768
1111	2	-0.17700043783657193
1111	3	-0.11662584477311455
1111	4	-0.15670680350366695
1111	5	-0.1958688799130357
1111	6	-0.10197710249611447
1111	7	-0.12297274397731428
1111	8	-0.10076760448633593
1111	9	-0.10201803768496376
1111	10	-0.044104795579720246
1111	11	-0.10772561833056926
1111	12	-0.0966344858577628
1111	13	-0.05808095198640473
1111	14	-0.0897479318026626
1111	15	-0.07738389180362049
1111	16	-0.07996485419364774
1111	17	-0.1120080987521419
1111	18	-0.10685795519819408
1111	19	-0.08574253314957048
1111	20	-0.017202299421185785
1111	21	-0.10702336083909358
1111	22	-0.08818680220136343
1111	23	-0.11069282645387091
1111	24	-0.14095761756347258
1111	25	0.008124800783079422
1112	1	-0.15415506060722706
1112	2	-0.1409145474032769
1112	3	-0.15860007833836676
1112	4	-0.14737566428805593
1112	5	-0.1309057120279391
1112	6	-0.09118432792581643
1112	7	-0.18142542399947814
1112	8	-0.13437839222676473
1112	9	-0.15120573345266308
1112	10	-0.15670709107632064
1112	11	-0.1358250680427986
1112	12	-0.12583108569990742
1112	13	-0.1395759910924923
1112	14	-0.13779186618895523
1112	15	-0.20055452468660206
1112	16	-0.14440571495005372
1112	17	-0.1423554004727876
1112	18	-0.10988375002588668
1112	19	-0.1147709658185434
1112	20	-0.17806059740312463
1112	21	-0.12947507528691204
1112	22	-0.13552059555989615
1112	23	-0.10950348953836908
1112	24	-0.0960181210988793
1112	25	0.03624282594807937
1113	1	-0.26999383515061837
1113	2	-0.19251813197593037
1113	3	-0.2902972274802771
1113	4	-0.2589388328185933
1113	5	-0.2598393410703963
1113	6	-0.2577795592722225
1113	7	-0.2772517956672189
1113	8	-0.2975565854866744
1113	9	-0.2662705748280345
1113	10	-0.3069467219984787
1113	11	-0.269838690565841
1113	12	-0.2708661134938666
1113	13	-0.28935474050855703
1113	14	-0.31795993197867745
1113	15	-0.25190676955684665
1113	16	-0.2835453889782755
1113	17	-0.25654535566989267
1113	18	-0.289105731849034
1113	19	-0.20119943026945464
1113	20	-0.22573965591082157
1113	21	-0.28196448525318374
1113	22	-0.2218942036482474
1113	23	-0.21192787696769955
1113	24	-0.188478699784568
1113	25	-0.13636703882644952
1114	1	-0.2679655148901965
1114	2	-0.2457137336104509
1114	3	-0.3123595769933516
1114	4	-0.2290686712978811
1114	5	-0.2334996945547161
1114	6	-0.22631858343555677
1114	7	-0.2293179763744888
1114	8	-0.3568743821304494
1114	9	-0.3331601633599206
1114	10	-0.36060481431681524
1114	11	-0.38406307090721015
1114	12	-0.28699112117850945
1114	13	-0.25577949841434133
1114	14	-0.38828928838165666
1114	15	-0.44073831165946586
1114	16	-0.38975228131253203
1114	17	-0.35351071487667696
1114	18	-0.30976956481321594
1114	19	-0.3803906135175623
1114	20	-0.3296396227231071
1114	21	-0.3042070299029831
1114	22	-0.31634782271994055
1114	23	-0.41934155442964993
1114	24	-0.4168736538447675
1114	25	-0.2946039033887402
1115	1	-0.26924015516694055
1115	2	-0.25107750963279685
1115	3	-0.2821475182329394
1115	4	-0.23938059692603275
1115	5	-0.25419775372119346
1115	6	-0.22639048863707245
1115	7	-0.25417404191079385
1115	8	-0.27558704232622944
1115	9	-0.3594081216695386
1115	10	-0.3524186957657349
1115	11	-0.31707230305526934
1115	12	-0.2754515412404075
1115	13	-0.3810541464024837
1115	14	-0.4971915421836357
1115	15	-0.428036113757976
1115	16	-0.4889158482774931
1115	17	-0.49576915297403445
1115	18	-0.461826074640193
1115	19	-0.46338600263801766
1115	20	-0.4534162748353481
1115	21	-0.5058221925932784
1115	22	-0.4255865861532584
1115	23	-0.47257445563084843
1115	24	-0.521292048367016
1115	25	-0.378216464839376
1116	1	-0.24877292784142924
1116	2	-0.21192884406437074
1116	3	-0.24413057970670593
1116	4	-0.2227840689508085
1116	5	-0.21984926672066507
1116	6	-0.25078113178787476
1116	7	-0.25352214040985643
1116	8	-0.32110075350362877
1116	9	-0.31420719674534325
1116	10	-0.3523890021094189
1116	11	-0.3529138845261244
1116	12	-0.28658833436921327
1116	13	-0.3157875758259223
1116	14	-0.35629195375436495
1116	15	-0.39595623448533956
1116	16	-0.363640238870758
1116	17	-0.34858130409571686
1116	18	-0.3292044727787342
1116	19	-0.3723651513507207
1116	20	-0.3983794010836661
1116	21	-0.3978831750583467
1116	22	-0.4452021240031314
1116	23	-0.4551940371470806
1116	24	-0.48215869235375663
1116	25	-0.32046002235064464
1201	1	0.3954783600906886
1201	2	0.38216205733957054
1201	3	0.39035626056438844
1201	4	0.29204258418766926
1201	5	0.3368919454568342
1201	6	0.3731946781557909
1201	7	0.40674518516587455
1201	8	0.41951683899264935
1201	9	0.45125682329522476
1201	10	0.4445651788983582
1201	11	0.4791380373638674
1201	12	0.5135466473715553
1201	13	0.447970357120532
1201	14	0.5375131421058441
1201	15	0.5718836560642444
1201	16	0.5101821464227783
1201	17	0.5080770139693769
1201	18	0.5270569704329304
1201	19	0.5411498020822236
1201	20	0.5743573140777087
1201	21	0.572280341883868
1201	22	0.5513157602460552
1201	23	0.5900552528988641
1201	24	0.6033895344190512
1201	25	0.5674124657557229
1202	1	0.338672803667599
1202	2	0.3554785322617313
1202	3	0.3555369387208991
1202	4	0.27280777732272554
1202	5	0.21836914346023661
1202	6	0.3390904227123599
1202	7	0.30932489280817066
1202	8	0.36308063938390234
1202	9	0.35786352789183695
1202	10	0.4023514013877474
1202	11	0.42262790768322134
1202	12	0.4649935213893528
1202	13	0.42022322647400046
1202	14	0.49036382129092004
1202	15	0.4544087573760649
1202	16	0.512858287020677
1202	17	0.5419931502713098
1202	18	0.5187038636245234
1202	19	0.4583491706155627
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2210	9	-0.18075813347970637
2210	10	-0.08695430254507386
2210	11	-0.10621633091653848
2210	12	-0.1156880477776312
2210	13	-0.11602450217601756
2210	14	-0.10145724791830446
2210	15	-0.09367067788968342
2210	16	-0.07933687531193812
2210	17	-0.10847299369735453
2210	18	-0.05916975428187499
2210	19	-0.09360804577849535
2210	20	-0.05788861535229728
2210	21	-0.08800728192095875
2210	22	-0.14874763010469394
2210	23	-0.11572472057130767
2210	24	-0.1829084725754484
2210	25	-0.24546479624006423
2211	1	-0.1664668821325428
2211	2	-0.1406286902435454
2211	3	-0.10656220911447534
2211	4	-0.0873227942093493
2211	5	-0.11784978593095019
2211	6	-0.15210174657811137
2211	7	-0.15619492651889288
2211	8	-0.24272044565490347
2211	9	-0.19190531499506136
2211	10	-0.19190087936407707
2211	11	-0.19512359708286908
2211	12	-0.19943749639683972
2211	13	-0.22198958296129234
2211	14	-0.22456726246881686
2211	15	-0.2631599418351058
2211	16	-0.16451441400663214
2211	17	-0.22125579613515356
2211	18	-0.20535051789607195
2211	19	-0.2272585846589621
2211	20	-0.17101317185164788
2211	21	-0.17794077403196695
2211	22	-0.21334315745627208
2211	23	-0.2712365610829721
2211	24	-0.26191544852031967
2211	25	-0.3054021620714309
2212	1	-0.35595384526146034
2212	2	-0.2954752628393784
2212	3	-0.29946404614793565
2212	4	-0.21966191059834017
2212	5	-0.1854408268026546
2212	6	-0.304791344292191
2212	7	-0.29617161343221454
2212	8	-0.3283826727012027
2212	9	-0.35694927534493814
2212	10	-0.32842501752868003
2212	11	-0.376171232440079
2212	12	-0.3758530160529361
2212	13	-0.2888205966947126
2212	14	-0.38528228368056716
2212	15	-0.3236342058031667
2212	16	-0.3540765176698457
2212	17	-0.3480865419964374
2212	18	-0.3368134259371516
2212	19	-0.32049472580272464
2212	20	-0.38720461995561095
2212	21	-0.3457731415112022
2212	22	-0.43967277560144424
2212	23	-0.44734866790944494
2212	24	-0.4516236617905065
2212	25	-0.4786739179693029
2213	1	-0.3182873885818083
2213	2	-0.2682269562754023
2213	3	-0.2845039008052943
2213	4	-0.23104391007119318
2213	5	-0.2658224379425391
2213	6	-0.259660195415897
2213	7	-0.2994158785189356
2213	8	-0.2720998717720917
2213	9	-0.3713863640208146
2213	10	-0.4008762547949303
2213	11	-0.34983337012726423
2213	12	-0.343851308535875
2213	13	-0.3572898220993608
2213	14	-0.4143292978688464
2213	15	-0.37099285494709733
2213	16	-0.40868951920185026
2213	17	-0.36054709560810494
2213	18	-0.37800993036938735
2213	19	-0.3539908045330975
2213	20	-0.3084136214613693
2213	21	-0.3173129657842537
2213	22	-0.3492396221877172
2213	23	-0.3248748689727279
2213	24	-0.3837758625535699
2213	25	-0.34892161212409434
2214	1	-0.36539912026741206
2214	2	-0.3927159746249167
2214	3	-0.36608963643494263
2214	4	-0.2666221112194549
2214	5	-0.3287575771322011
2214	6	-0.3684451999994849
2214	7	-0.40204616103483204
2214	8	-0.42242692913194446
2214	9	-0.4583923852235472
2214	10	-0.4220587648645875
2214	11	-0.4174498489851928
2214	12	-0.4220490929504521
2214	13	-0.41478024185190776
2214	14	-0.5041275181896765
2214	15	-0.46938044174864046
2214	16	-0.4288453808098893
2214	17	-0.47201824439248696
2214	18	-0.5241178006849125
2214	19	-0.4915468952854596
2214	20	-0.511115296537163
2214	21	-0.5483994658117552
2214	22	-0.5546206081415548
2214	23	-0.5605880275165873
2214	24	-0.555095947334415
2214	25	-1.4822914949920971
2215	1	-0.503668526073195
2215	2	-0.5614141663751084
2215	3	-0.5682504181812718
2215	4	-0.39444289557162904
2215	5	-0.5113692534867695
2215	6	-0.5549126165775675
2215	7	-0.502848844031476
2215	8	-0.5505797464831161
2215	9	-0.6498380495242841
2215	10	-0.6635203285458147
2215	11	-0.655423710491597
2215	12	-0.6469810412081145
2215	13	-0.7029592124504376
2215	14	-0.7883643266747171
2215	15	-0.7219320248447545
2215	16	-0.6657327887203763
2215	17	-0.7932863930504439
2215	18	-0.7773791522539
2215	19	-0.7123427817793697
2215	20	-0.7969513740952578
2215	21	-0.8119085531296619
2215	22	-0.8379355505644952
2215	23	-0.8216215844671737
2215	24	-0.7736096657224536
2215	25	-0.8118483285233538
2216	1	-0.39069084326354897
2216	2	-0.3006201672928448
2216	3	-0.28726015197873167
2216	4	-0.27233387161325595
2216	5	-0.28726238332921383
2216	6	-0.3050612275102935
2216	7	-0.3369486749352788
2216	8	-0.3337500730244116
2216	9	-0.3500757702904096
2216	10	-0.4116727194315928
2216	11	-0.4862027872635153
2216	12	-0.4194924598458343
2216	13	-0.43652445053449185
2216	14	-0.4760945022599188
2216	15	-0.46080308094695965
2216	16	-0.4720308999587388
2216	17	-0.5101281208353043
2216	18	-0.48348255539816876
2216	19	-0.4423763859316594
2216	20	-0.42838085030737005
2216	21	-0.4645900899815629
2216	22	-0.5189375685532411
2216	23	-0.5674923514073669
2216	24	-0.542772581642204
2216	25	-0.6842117105815362
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107705.0
3	8.0
4	1821.0
5	15820.0
6	19612.0
7	15437.0
8	12579.0
9	10920.0
10	9701.0
11	9001.0
12	8167.0
13	7847.0
14	7726.0
15	8108.0
16	8464.0
17	9183.0
18	10456.0
19	12349.0
20	16231.0
21	21025.0
22	26827.0
23	32563.0
24	39324.0
25	46559.0
26	53816.0
27	62238.0
28	74255.0
29	92971.0
30	121560.0
31	163039.0
32	216815.0
33	296943.0
34	415756.0
35	595025.0
36	896291.0
37	1616982.0
38	4917063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.216964159173976	18.362858068981858	13.560390247305367	25.859787524538795
2	16.867722677075868	21.11892226456627	36.55789157574799	25.455463482609876
3	18.296086558393185	24.331110264469505	29.070020756971083	28.302782420166228
4	13.459435225259828	17.21410861935912	36.386639224062264	32.93981693131878
5	15.436166545063863	35.517807461334314	33.0197217032945	16.026304290307323
6	32.350744824180495	36.85355786004282	16.359886062469876	14.435811253306813
7	28.997802842069298	31.021933081924036	21.193912120099	18.786351955907666
8	27.01553498317698	32.296124392112404	19.772317006340753	20.916023618369863
9	27.522515139987558	14.805757580769571	18.83773812620507	38.8339891530378
10	16.50789947223263	26.341342752925534	30.851479658936675	26.29927811590516
11	35.75768280255518	22.480012494008108	22.47170253514892	19.290602168287794
12	24.952863496836432	24.261755309152417	28.10087326943557	22.68450792457558
13	29.31355451217176	20.7209863103307	24.665343820970516	25.300115356527026
14	23.93901681396421	20.242068979338264	25.362933124912324	30.455981081785204
15	24.922957235717142	26.98301887794472	22.951203560535546	25.14282032580259
16	25.062594134192885	26.474179913382766	23.6440843470425	24.819141605381848
17	24.743540902092334	25.403173200998726	24.791787617457956	25.061498279450987
18	26.084785191289335	24.36862816457472	25.576351001457336	23.97023564267861
19	26.899746467676405	24.661952726937884	25.05430008475793	23.38400072062778
20	25.94373043273068	24.594591480256252	25.10133522110983	24.360342865903238
21	25.983688252172882	24.57503475141553	25.070216746830642	24.371060249580946
22	25.401420374329696	24.383914287769787	25.48963516311219	24.725030174788326
23	24.477439179942483	24.070031616670565	25.687577916946974	25.76495128643998
24	24.631330536695202	25.221706684055977	25.429210072306045	24.717752706942772
25	24.787375546971155	24.513539997822402	25.622499712747633	25.076584742458806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1566.0
1	1566.0
2	2474.0
3	3382.0
4	3382.0
5	3382.0
6	8033.5
7	12685.0
8	12685.0
9	12685.0
10	17315.5
11	21946.0
12	21946.0
13	21946.0
14	31233.5
15	40521.0
16	40521.0
17	40521.0
18	74310.0
19	108099.0
20	108099.0
21	108099.0
22	155237.0
23	202375.0
24	202375.0
25	202375.0
26	277458.5
27	352542.0
28	352542.0
29	352542.0
30	424463.0
31	496384.0
32	496384.0
33	496384.0
34	584764.0
35	673144.0
36	673144.0
37	673144.0
38	752326.0
39	831508.0
40	831508.0
41	831508.0
42	927553.0
43	1023598.0
44	1023598.0
45	1023598.0
46	1094506.5
47	1165415.0
48	1165415.0
49	1165415.0
50	1195203.0
51	1224991.0
52	1224991.0
53	1224991.0
54	1154818.5
55	1084646.0
56	1084646.0
57	1084646.0
58	1002878.5
59	921111.0
60	921111.0
61	921111.0
62	808039.5
63	694968.0
64	694968.0
65	694968.0
66	583195.0
67	471422.0
68	471422.0
69	471422.0
70	371699.5
71	271977.0
72	271977.0
73	271977.0
74	220093.0
75	168209.0
76	168209.0
77	168209.0
78	138260.0
79	108311.0
80	108311.0
81	108311.0
82	83611.0
83	58911.0
84	58911.0
85	58911.0
86	44193.5
87	29476.0
88	29476.0
89	29476.0
90	20660.5
91	11845.0
92	11845.0
93	11845.0
94	6428.0
95	1011.0
96	1011.0
97	1011.0
98	577.5
99	144.0
100	144.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0313521179512969
2	0.00298591599536161
3	0.00890764872441769
4	0.16636962814424217
5	0.023516593426556036
6	0.09127083490519768
7	0.05493885034418694
8	0.021953496462541234
9	0.04130183131839113
10	0.026903303515254776
11	0.042133479062065674
12	0.03318575092831427
13	0.006923717962398901
14	0.0038776828530367215
15	0.0034067497933655956
16	0.004188298275372997
17	0.0023346255936887756
18	0.003316571122364741
19	0.0
20	0.0019238116480182184
21	1.8035734200170798E-4
22	1.0019852333428222E-5
23	9.017867100085399E-5
24	0.001092163904343676
25	0.15111941289276443
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	9980187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.3754117111472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.56551456671438	33.29246026062654
2	12.843041917310071	10.884583777390711
3	3.440089371467748	4.373256103172622
4	1.4420326945442712	2.4442691652899384
5	0.7469474232906623	1.582610229426118
6	0.5041209608235975	1.2817399960271432
7	0.3587855530031458	1.064257986716395
8	0.24910987745902674	0.8444906894911749
9	0.1964142217031643	0.7490820159536519
>10	1.3354064216526127	11.15794356654343
>50	0.16395511933818763	4.858590454310226
>100	0.12651409487042056	10.648315519782267
>500	0.015626453431477498	4.581279811492215
>1k	0.011657805932607521	9.499541854222208
>5k	6.885465245862985E-4	1.887196978417836
>10k+	9.497193442569633E-5	0.8503815911375574
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	35738	0.35808948269205776	No Hit
TATCAACGCAGAGTACTTTTTTTTT	21056	0.21097801073266462	No Hit
GTACAAAGGGCAGGGACTTAATCAA	14083	0.14110958041166966	No Hit
GGTATCAACGCAGAGTACTTTTTTT	13644	0.13671086523729467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0039704666856444E-5
2	1.0019852333428222E-5	0.0	0.0	0.0	2.0039704666856444E-5
3	1.0019852333428222E-5	0.0	0.0	0.0	2.0039704666856444E-5
4	1.0019852333428222E-5	0.0	0.0	1.0019852333428222E-5	2.0039704666856444E-5
5	2.0039704666856444E-5	0.0	0.0	1.0019852333428222E-5	2.0039704666856444E-5
6	2.0039704666856444E-5	0.0	0.0	1.0019852333428222E-5	4.007940933371289E-5
7	2.0039704666856444E-5	0.0	0.0	1.0019852333428222E-5	4.007940933371289E-5
8	2.0039704666856444E-5	0.0	0.0	2.0039704666856444E-5	4.007940933371289E-5
9	2.0039704666856444E-5	0.0	0.0	2.0039704666856444E-5	6.0119114000569325E-5
10	2.0039704666856444E-5	0.0	0.0	4.007940933371289E-5	6.0119114000569325E-5
11	2.0039704666856444E-5	0.0	0.0	5.009926166714111E-5	6.0119114000569325E-5
12	3.0059557000284662E-5	0.0	0.0	5.009926166714111E-5	1.2023822800113865E-4
13	4.007940933371289E-5	0.0	0.0	6.0119114000569325E-5	1.903771943351362E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6390	0.0	16.327734	1
GTATCAA	21525	0.0	13.61704	1
AACCGCG	735	0.0	11.369441	7
CGTCGTA	1320	0.0	11.149344	10
AGAACCG	990	0.0	10.845361	5
TAGATCG	450	0.0	10.7685795	5
ACGGTAT	1285	0.0	10.197039	9
CCGTCGT	1450	0.0	10.1499	9
CGCGCTA	790	0.0	10.092278	16
CCGACCA	1450	0.0	9.887967	9
GCGTTAT	655	0.0	9.882887	1
ATACCGT	1560	0.0	9.864861	6
GCGCGCT	845	0.0	9.772363	15
GTCGTAG	1450	0.0	9.756854	11
ACACCGT	560	0.0	9.66913	6
CGACCAT	1365	0.0	9.668825	10
CGAGCCG	1770	0.0	9.652421	15
TACCGTC	1555	0.0	9.648737	7
GAGCGTT	570	0.0	9.496067	7
CTCGACG	480	0.0	9.491595	13
>>END_MODULE