FastQCFastQC Report
Thu 2 Feb 2017
SRR4050270_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050270_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623460
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA114771.8408558688608732No Hit
CTTCCGTACTCTGCGTTGATACCAC60300.9671831392551246No Hit
GAGTACGGAAGCAGTGGTATCAACG54380.8722291726814873No Hit
TCCGTACTCTGCGTTGATACCACTG42420.6803964969685304No Hit
GCTTCCGTACTCTGCGTTGATACCA42140.6759054309819396No Hit
GTATCAACGCAGAGTACTTTTTTTT30490.4890450068969942No Hit
GTATCAACGCAGAGTACGGAAGCAG29050.4659480961088121No Hit
GCGTTGATACCACTGCTTCCGTACT25130.40307317229653866No Hit
CCCATGTACTCTGCGTTGATACCAC22790.36554069226574276No Hit
GTACATGGAAGCAGTGGTATCAACG22170.35559618900971995No Hit
GTACATGGGAAGCAGTGGTATCAAC20150.3231963558207423No Hit
TATCAACGCAGAGTACGGAAGCAGT19220.30827960093670803No Hit
TATCAACGCAGAGTACTTTTTTTTT17930.28758861835562827No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17860.2864658518589805No Hit
TCCATGTACTCTGCGTTGATACCAC16510.2648124979950598No Hit
ACTCTGCGTTGATACCACTGCTTCC14170.22728001796426395No Hit
GTATCAACGCAGAGTACGGGAAGCA13340.2139672152182979No Hit
GGTATCAACGCAGAGTACGGAAGCA13180.21140089179738875No Hit
GGTATCAACGCAGAGTACTTTTTTT12360.19824848426522954No Hit
CAGTGGTATCAACGCAGAGTACGGA12270.19680492734096816No Hit
GTATCAACGCAGAGTACATGGGAAG11670.18718121451255895No Hit
AAGCAGTGGTATCAACGCAGAGTAC10970.17595354954608153No Hit
ATACCACTGCTTCCGTACTCTGCGT10930.17531196869085428No Hit
ACGCAGAGTACGGAAGCAGTGGTAT10860.17418920219420653No Hit
AAAAAGTACTCTGCGTTGATACCAC10180.16328232765534276No Hit
GAAGCAGTGGTATCAACGCAGAGTA9600.1539794052545472No Hit
GTACGGGAAGCAGTGGTATCAACGC9560.15333782439931992No Hit
GTATCTGATCGTCTTCGAACCTCCG8750.1403458120809675No Hit
CATGTACTCTGCGTTGATACCACTG8580.13761909344625156No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8360.13409039874250153No Hit
GTCCTATTCCATTATTCCTAGCTGC8300.1331280274596606No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8190.1313636801077856No Hit
CTATTGGAGCTGGAATTACCGCGGC8130.13040130882494466No Hit
GAGTACGGGAAGCAGTGGTATCAAC7930.12719340454880826No Hit
GAATAGGACCGCGGTTCTATTTTGT7900.1267122189073878No Hit
GATTAAGAGGGACGGCCGGGGGCAT7640.1225419433484105No Hit
GTGGTATCAACGCAGAGTACGGAAG7480.11997561992750136No Hit
GTACTCTGCGTTGATACCACTGCTT7390.11853206300323998No Hit
GAGTACATGGAAGCAGTGGTATCAA7220.11580534436852405No Hit
CTTTAATATACGCTATTGGAGCTGG7010.11243704487858082No Hit
GAATAACGCCGCCGCATCGCCAGTC7010.11243704487858082No Hit
TATCAACGCAGAGTACATGGGAAGC6970.11179546402335355No Hit
TATCAACGCAGAGTACGGGAAGCAG6930.11115388316812627No Hit
ATACAGGACTCTTTCGAGGCCCTGT6850.10987072145767171No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6730.10794597889198987No Hit
GAACTACGACGGTATCTGATCGTCT6730.10794597889198987No Hit
ATCAACGCAGAGTACGGAAGCAGTG6640.10650242196772848No Hit
CTCTTAATCATGGCCTCAGTTCCGA6500.10425688897443301No Hit
GCCCATGTACTCTGCGTTGATACCA6480.10393609854681936No Hit
GAGTACATGGGAAGCAGTGGTATCA6360.10201135598113752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCA400.005227180714.2693146
CGGACAT602.5308156E-514.2693135
AGAGTGT852.6461748E-713.4299426
GTATTAG1500.013.2644891
TAGAGTG751.4547872E-512.6838345
CTCGTAG604.0394388E-412.68383410
ACGGTAT1301.9281288E-1012.4399149
AGGCCCG1850.012.34102810
CCGAGCC1950.012.18910614
GCCGCCT1950.012.18615618
ACAGTCT550.003033933212.1072958
GCTCGTA550.003033933212.1072959
TGTTCAA951.0195963E-612.01626410
TGCGGTA951.0265794E-612.00947815
ATTCCGG802.9392055E-511.8432931
AAATTAG802.9392055E-511.8432931
GTATAAA802.9392055E-511.8432931
CAATACG802.9830295E-511.8261794
CGAGCCG1850.011.82013915
GCTCTTA1052.805191E-711.7304991
Produced by FastQC (version 0.11.5)