##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050270_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623460
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.092854393224908	31.0	30.0	34.0	26.0	34.0
2	30.18791903249607	31.0	31.0	34.0	26.0	34.0
3	30.30980656337215	31.0	31.0	34.0	26.0	34.0
4	33.80528021043852	37.0	35.0	37.0	28.0	37.0
5	33.82841240817374	37.0	35.0	37.0	30.0	37.0
6	33.75424566130947	37.0	35.0	37.0	29.0	37.0
7	33.60038174060886	37.0	35.0	37.0	28.0	37.0
8	33.45385429698778	37.0	35.0	37.0	27.0	37.0
9	34.82071985371957	39.0	35.0	39.0	27.0	39.0
10	34.75489686587752	39.0	35.0	39.0	27.0	39.0
11	34.60799890931255	39.0	35.0	39.0	27.0	39.0
12	34.52544990857473	39.0	35.0	39.0	26.0	39.0
13	34.52701696981362	39.0	35.0	39.0	26.0	39.0
14	35.63093542488692	40.0	36.0	41.0	26.0	41.0
15	35.607129567253715	40.0	36.0	41.0	26.0	41.0
16	35.615104417284186	40.0	36.0	41.0	27.0	41.0
17	35.63411926988098	40.0	36.0	41.0	27.0	41.0
18	35.59505661951047	40.0	36.0	41.0	26.0	41.0
19	35.61339300035287	40.0	36.0	41.0	26.0	41.0
20	35.56437943091778	40.0	36.0	41.0	25.0	41.0
21	35.514899111410514	40.0	36.0	41.0	25.0	41.0
22	35.45656658005325	40.0	36.0	41.0	26.0	41.0
23	35.36978314567094	40.0	36.0	41.0	25.0	41.0
24	35.30307958810509	40.0	36.0	41.0	25.0	41.0
25	35.11113463574247	40.0	36.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7728359571170209
1101	2	0.8244509661715256
1101	3	0.6666452247491144
1101	4	0.7046848437209121
1101	5	0.9356139357127375
1101	6	0.8644975480166792
1101	7	0.7297344456284947
1101	8	0.9427371616883917
1101	9	1.0984798859047231
1101	10	1.1490245025025203
1101	11	0.789828149780476
1101	12	0.9273353489710203
1101	13	0.8810617249900048
1101	14	1.268473328429657
1101	15	1.083358538543017
1101	16	1.119057468376198
1101	17	1.1228806526224844
1101	18	1.1790234485026758
1101	19	1.2113577460616938
1101	20	1.31330502467884
1101	21	1.3045892037279714
1101	22	1.4070569848499446
1101	23	1.3643622539679185
1101	24	1.190810188985452
1101	25	1.2351252644954016
1102	1	0.9060875149404843
1102	2	1.0550431844140071
1102	3	0.9029584576621197
1102	4	0.9691124969273304
1102	5	0.8625606017252068
1102	6	0.8817440030483255
1102	7	0.7791701946103871
1102	8	0.7624982136485485
1102	9	0.9154442154273212
1102	10	0.9902369062118623
1102	11	0.7950462450653006
1102	12	1.0404431454481298
1102	13	0.9940493114676059
1102	14	1.1263802024790195
1102	15	1.0030680719319562
1102	16	1.2169574732827328
1102	17	1.143053853152388
1102	18	1.0683100389545643
1102	19	1.039158148397199
1102	20	1.0249457685088785
1102	21	1.0701453589705991
1102	22	1.388144762682245
1102	23	1.4819693389980912
1102	24	1.343523500402945
1102	25	1.3512996425926502
1103	1	0.5667991670883126
1103	2	0.5253947610722172
1103	3	0.4570285230281321
1103	4	0.4741205583188872
1103	5	0.5630505343594692
1103	6	0.4692694682968579
1103	7	0.45707948208335836
1103	8	0.2874785288552033
1103	9	0.48419351239898845
1103	10	0.7450518691544588
1103	11	0.9243772120573439
1103	12	0.5843183626057709
1103	13	0.6518113691846708
1103	14	0.694208376105145
1103	15	0.6300431128638238
1103	16	0.8192896526080702
1103	17	0.7992398775740313
1103	18	0.8513048771643099
1103	19	0.7255025260452754
1103	20	0.7374140541196041
1103	21	0.7950856796764967
1103	22	0.7457605905059168
1103	23	0.877000022279141
1103	24	0.6607148865873427
1103	25	0.8125924648295921
1104	1	0.6790341223788623
1104	2	0.760346356113832
1104	3	0.7681506385323793
1104	4	0.9368778732633913
1104	5	0.7406983946003862
1104	6	1.0629191122349297
1104	7	0.9927454182718378
1104	8	0.9641033500851464
1104	9	1.090782351727043
1104	10	1.1128556427579568
1104	11	1.2375823606351233
1104	12	1.055611062628067
1104	13	1.1028551646162867
1104	14	1.0971206805359301
1104	15	1.0986085300801989
1104	16	1.0598830521894982
1104	17	1.3332650094727683
1104	18	1.1582399853255936
1104	19	1.130373448209312
1104	20	1.0427403857025013
1104	21	0.9830747951819774
1104	22	1.1026096549887896
1104	23	1.1670589902230972
1104	24	1.309810658067164
1104	25	1.3997959159386753
1105	1	0.5223748066878677
1105	2	0.7316656175794449
1105	3	0.6637512705253528
1105	4	0.6905068208327947
1105	5	0.539899811333882
1105	6	0.7421421045593704
1105	7	0.743578369736305
1105	8	0.6368250249619933
1105	9	0.8914515769151166
1105	10	0.9717968635927221
1105	11	0.6853964000439916
1105	12	0.8342488409150093
1105	13	0.9587843947686565
1105	14	1.212005928941629
1105	15	1.2362722563565995
1105	16	1.2097582288038495
1105	17	1.3209306450934974
1105	18	1.2561296825035768
1105	19	1.3546390666459445
1105	20	1.2337989261996967
1105	21	1.2077664360724967
1105	22	1.139566207858529
1105	23	1.1692344494204079
1105	24	1.1693008153971363
1105	25	1.1032974147739765
1106	1	0.2829670544936782
1106	2	0.4664095674894746
1106	3	0.4372550437984586
1106	4	0.2895609778597148
1106	5	0.19261408917898848
1106	6	0.3592122635693187
1106	7	0.4028339057063448
1106	8	0.3363838916947586
1106	9	0.36544720689565224
1106	10	0.4716419390581805
1106	11	0.7329421658990825
1106	12	0.6447795779426571
1106	13	0.4461941942618779
1106	14	0.49005300763869997
1106	15	0.4970779557433218
1106	16	0.7953901831940655
1106	17	0.5939650544064108
1106	18	0.5165061835501774
1106	19	0.6562633643213829
1106	20	0.6597748360083671
1106	21	0.581605075858036
1106	22	0.8036381754689756
1106	23	0.7867257736464737
1106	24	0.6301875345774803
1106	25	0.7449110628221547
1107	1	0.08259952477046895
1107	2	0.026022283883516906
1107	3	0.04090077739652287
1107	4	0.027546552150099046
1107	5	-0.0022404946960676853
1107	6	0.023268595730186803
1107	7	-0.0853154443976365
1107	8	-0.34038816260826366
1107	9	-0.27001638784067694
1107	10	-0.2132567551026625
1107	11	-0.08538594340573269
1107	12	-0.14701181030512345
1107	13	-0.1948730529270577
1107	14	-0.015794208844368995
1107	15	0.0963832103433262
1107	16	-0.1772130627585753
1107	17	-0.19663706947193305
1107	18	0.1742399294305912
1107	19	0.19950857786825082
1107	20	0.20978609034632711
1107	21	0.37742450924411486
1107	22	0.08545687648320666
1107	23	0.13357439104265723
1107	24	0.13362262728298901
1107	25	0.10195241495527085
1108	1	0.1182733136199694
1108	2	0.1002534241916031
1108	3	0.14129970635898204
1108	4	0.11632667508092709
1108	5	0.11757044766520153
1108	6	0.024336381132421536
1108	7	-0.048294186253734495
1108	8	0.011820581307532052
1108	9	-0.2046646690855951
1108	10	-0.01912252851534646
1108	11	0.11239826637886807
1108	12	-0.0223855351959088
1108	13	-0.3188662451175901
1108	14	-0.08081312544869945
1108	15	0.09734584327447493
1108	16	-0.005410185700242209
1108	17	0.14304226969356648
1108	18	0.24345193632512263
1108	19	-0.12694865109313014
1108	20	-0.08207380753653126
1108	21	0.15579345349161855
1108	22	-0.0321610406306263
1108	23	-0.1818786860221806
1108	24	0.11264199918668538
1108	25	0.03975093414830866
1109	1	-0.30427622133609944
1109	2	-0.5037415552649342
1109	3	-0.31964089460436185
1109	4	-0.5232959259467833
1109	5	-0.3355464730681419
1109	6	-0.4469456811523571
1109	7	-0.5674280352297814
1109	8	-0.4821579848338402
1109	9	-0.5141200259411036
1109	10	-0.43916715556835584
1109	11	-0.47491949754831353
1109	12	-0.46724430559186203
1109	13	-0.5697968693599478
1109	14	-0.6566115313188732
1109	15	-0.7359549494993729
1109	16	-0.4367295802312796
1109	17	-0.6758040780756147
1109	18	-0.5539841536584618
1109	19	-0.5114632782534088
1109	20	-0.5071228865671813
1109	21	-0.519493243140964
1109	22	-0.6901000431730822
1109	23	-0.7178903577129674
1109	24	-0.6253735988265561
1109	25	-0.5487050341836479
1110	1	-0.13860274025294572
1110	2	-0.12010046366564708
1110	3	-0.054565910170015997
1110	4	0.013530576811945139
1110	5	0.039725791201092875
1110	6	-0.2715697894936042
1110	7	-0.43310560970254386
1110	8	-0.2067041033310062
1110	9	-0.2942145186085483
1110	10	-0.4013000700833018
1110	11	-0.14337002031725632
1110	12	-0.19216206077700804
1110	13	-0.386335456020241
1110	14	-0.19150660050792823
1110	15	-0.03644852610931082
1110	16	-0.06913134426675782
1110	17	-0.17357210353218733
1110	18	-0.06565139124526098
1110	19	-0.012050356499628379
1110	20	0.06286653525231145
1110	21	0.039529126156025995
1110	22	-0.09670181884240492
1110	23	-0.007094796670834569
1110	24	0.14543105384933597
1110	25	-0.10966530029705979
1111	1	-0.4080206167660485
1111	2	-0.381624171575055
1111	3	-0.39815594687821587
1111	4	-0.4403174334511064
1111	5	-0.27466280766336837
1111	6	-0.540397032313912
1111	7	-0.5723681432035406
1111	8	-0.5125253381999855
1111	9	-0.53082999702184
1111	10	-0.5862319249271266
1111	11	-0.6512578380210243
1111	12	-0.7227861002033933
1111	13	-0.7632931209213538
1111	14	-0.6034513730822084
1111	15	-0.5248517828507957
1111	16	-0.6126604123357069
1111	17	-0.6210692138727225
1111	18	-0.4843725740368612
1111	19	-0.470490067406665
1111	20	-0.3990725215120321
1111	21	-0.7392753804425567
1111	22	-0.6337021617909997
1111	23	-0.727566287481082
1111	24	-0.6126631681019106
1111	25	-0.5710137233686083
1112	1	-0.36442794433902037
1112	2	-0.3764748504748141
1112	3	-0.29814489287943147
1112	4	-0.4840912686359857
1112	5	-0.4996116829189887
1112	6	-0.834393482752084
1112	7	-0.5572763643080805
1112	8	-0.6967003107185192
1112	9	-0.6461938271095988
1112	10	-0.46198497859429466
1112	11	-0.5885294654766966
1112	12	-0.5734066594129104
1112	13	-0.7272540143301001
1112	14	-0.8295701298758118
1112	15	-0.7825051417311499
1112	16	-0.7028364779718927
1112	17	-0.8167587684579871
1112	18	-0.809295656628656
1112	19	-0.7530149235511487
1112	20	-1.0406220464971412
1112	21	-0.8617168403073308
1112	22	-0.7668844238076176
1112	23	-0.8126083847931156
1112	24	-0.8764852724148184
1112	25	-0.7188210398875867
1113	1	-0.27338739884302754
1113	2	-0.10210444046084177
1113	3	-0.22002518901715362
1113	4	-0.33019777117812765
1113	5	-0.2606677885295525
1113	6	-0.4564026206028018
1113	7	-0.18960275009981586
1113	8	-0.04466609828826762
1113	9	0.028059634272572964
1113	10	-0.12522643787535515
1113	11	0.04083554309622883
1113	12	0.17864539263914736
1113	13	-0.08742804975165797
1113	14	0.03928486373165896
1113	15	0.07403258411351032
1113	16	0.008992182371613922
1113	17	-0.14656679430561326
1113	18	-0.12690633661557627
1113	19	-0.10112441918540327
1113	20	-0.14952302668525164
1113	21	-0.28369822763525576
1113	22	-0.14853339951599764
1113	23	-0.38525727130338794
1113	24	-0.33660777115117213
1113	25	-0.29157498194845743
1114	1	-0.6214322231470639
1114	2	-0.6639655125938226
1114	3	-0.679800086229374
1114	4	-0.6012798149622895
1114	5	-0.6892383760144654
1114	6	-0.9100122134748716
1114	7	-1.047455344080717
1114	8	-0.9510884947399987
1114	9	-1.0037972184097157
1114	10	-0.9918289950587251
1114	11	-0.7474098523055517
1114	12	-0.9524745280744327
1114	13	-0.775795122336028
1114	14	-0.8679127341252482
1114	15	-0.934227726555811
1114	16	-0.9190298765240641
1114	17	-0.7793735140261475
1114	18	-0.9074006992332002
1114	19	-1.1105161313992369
1114	20	-1.0430456792478253
1114	21	-0.9346073720680295
1114	22	-1.1333728150730167
1114	23	-0.8599778553538613
1114	24	-0.8123567236480085
1114	25	-0.7456536207340392
1115	1	-0.4718878314105588
1115	2	-0.5580690660496117
1115	3	-0.3887072999466916
1115	4	-0.3142408356787527
1115	5	-0.5222654046018391
1115	6	-0.5216049660800692
1115	7	-0.7299276563768515
1115	8	-0.7411671076124975
1115	9	-0.7973080481267303
1115	10	-0.6465472107293451
1115	11	-0.4399488460222969
1115	12	-0.624267228720079
1115	13	-0.6687411325162458
1115	14	-0.5041641413630273
1115	15	-0.5728673485982085
1115	16	-0.687624178293575
1115	17	-0.683421547742121
1115	18	-0.521686688437164
1115	19	-0.4443042711511822
1115	20	-0.5453607657989679
1115	21	-0.44452683476781374
1115	22	-0.6319708694765893
1115	23	-0.440731653693085
1115	24	-0.4729049931063827
1115	25	-0.6747721366153812
1116	1	-0.5774680872857054
1116	2	-0.5553106956657992
1116	3	-0.6529482944118037
1116	4	-0.7383221350163822
1116	5	-0.5918235209252245
1116	6	-0.5531084594109856
1116	7	-0.6331578532736799
1116	8	-0.6326923775517059
1116	9	-0.7564133924693479
1116	10	-0.7167041837807915
1116	11	-0.5747006834236217
1116	12	-0.8437845139029108
1116	13	-0.5428088214736917
1116	14	-0.5899276836913074
1116	15	-0.8081363804464985
1116	16	-0.7429186161848733
1116	17	-0.6455211757250652
1116	18	-0.8959449227171774
1116	19	-0.7931110944884452
1116	20	-0.9664750022566366
1116	21	-1.0210443921582524
1116	22	-0.9016378688414335
1116	23	-1.0118141325969958
1116	24	-1.1302137672648058
1116	25	-0.9252938678557356
1201	1	0.753732332568557
1201	2	0.6558901278944838
1201	3	0.5891743918480614
1201	4	0.47992134008504905
1201	5	0.6767742062098776
1201	6	0.9063029372100004
1201	7	0.7454356349481941
1201	8	0.9059422886843436
1201	9	0.8465699950278776
1201	10	0.8231900452416383
1201	11	0.6892947950689461
1201	12	0.6816691507384931
1201	13	0.7416868487792314
1201	14	0.860624384292791
1201	15	0.740219526781857
1201	16	0.8130552236649464
1201	17	0.8510937492865409
1201	18	0.8033526288058042
1201	19	0.8859123406460938
1201	20	1.0077059791527034
1201	21	0.8972354164850955
1201	22	0.7952576318549589
1201	23	0.8313541522579726
1201	24	0.8301544126265981
1201	25	0.9040091336985299
1202	1	0.4678316471396613
1202	2	0.5109451074440585
1202	3	0.42495021958661283
1202	4	0.4703262725494497
1202	5	0.41759728309691724
1202	6	0.5035724658429359
1202	7	0.6298684811881756
1202	8	0.3836881037262998
1202	9	0.3787823421181997
1202	10	0.30278412890800155
1202	11	0.6450815859217727
1202	12	0.6033742429645272
1202	13	0.5888310512126651
1202	14	0.5459127282534695
1202	15	0.5377532945793533
1202	16	0.41144917427471484
1202	17	0.48014225403957056
1202	18	0.4797580391495515
1202	19	0.4260893434056072
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2210	21	0.337774359183868
2210	22	0.3646267077035148
2210	23	0.30911073692600866
2210	24	0.28249688656734406
2210	25	0.42522010415355993
2211	1	0.09787017467551706
2211	2	0.023677540722353285
2211	3	0.006278933932453157
2211	4	-0.0014504188309558685
2211	5	0.056607222553644476
2211	6	0.24065171169609556
2211	7	0.4523212930507725
2211	8	0.21015878718535674
2211	9	0.3308279708077251
2211	10	0.36332092432354557
2211	11	0.5008375134425194
2211	12	0.46969016875854663
2211	13	0.4520626157327996
2211	14	0.6603675690171116
2211	15	0.5350542086640218
2211	16	0.6797670210495141
2211	17	0.494716886301255
2211	18	0.4874279326322437
2211	19	0.3144166871640408
2211	20	0.5402065432866721
2211	21	0.4298971617544325
2211	22	0.2549344099515167
2211	23	0.23766278189596335
2211	24	0.48612509915078306
2211	25	0.277751652207499
2212	1	-0.6075570992620314
2212	2	-0.6540547098187979
2212	3	-0.4796018701738731
2212	4	-0.46054641555694076
2212	5	-0.47615611635143296
2212	6	-0.4451955633262159
2212	7	-0.42604801008666726
2212	8	-0.49122724597884826
2212	9	-0.5627664949211137
2212	10	-0.4893512943649725
2212	11	-0.6829896342678907
2212	12	-0.7334457867463868
2212	13	-0.6261420004331484
2212	14	-0.799528194977583
2212	15	-0.8242178157530375
2212	16	-0.8228950896063267
2212	17	-0.8804637023314044
2212	18	-0.7676583801053525
2212	19	-0.9598719292188633
2212	20	-0.6852656990694328
2212	21	-0.8037847754377054
2212	22	-0.8410041332097862
2212	23	-0.7479857839960857
2212	24	-0.8985807077907282
2212	25	-0.9012532243019322
2213	1	-0.5383562078626589
2213	2	-0.7642441226371517
2213	3	-0.42854118512543593
2213	4	-0.47219208621657316
2213	5	-0.4244671742484698
2213	6	-0.5533407537224733
2213	7	-0.9053527644076027
2213	8	-0.7852773270412001
2213	9	-0.8437902674806068
2213	10	-0.6654427716474629
2213	11	-0.7698495138487687
2213	12	-0.6841677094803131
2213	13	-0.6724506560585368
2213	14	-0.39699015105305335
2213	15	-0.6443004630640417
2213	16	-0.5955557868761758
2213	17	-0.5106752282704008
2213	18	-0.5772659390498092
2213	19	-0.5285476817428716
2213	20	-0.4339141825638393
2213	21	-0.4200323812818354
2213	22	-0.4802451834111494
2213	23	-0.41819952506633484
2213	24	-0.36468156118913697
2213	25	-0.32276023920250907
2214	1	-0.8402308223158101
2214	2	-0.6635316701403298
2214	3	-0.7409483682916012
2214	4	-0.8163207313002303
2214	5	-0.7153560834460038
2214	6	-0.8996989067531587
2214	7	-0.7457820636833432
2214	8	-0.6087368531898392
2214	9	-0.6445815428886164
2214	10	-0.7963687694998214
2214	11	-0.9792442909741368
2214	12	-1.0539633030130275
2214	13	-0.7483719702306644
2214	14	-0.89873415556157
2214	15	-1.0606815394727604
2214	16	-1.0100310242968789
2214	17	-1.0951794816613045
2214	18	-1.042877064123104
2214	19	-1.0082860049091877
2214	20	-0.87666856732627
2214	21	-0.955106672827597
2214	22	-1.003863497743211
2214	23	-0.8734132608153828
2214	24	-0.9017645042157838
2214	25	-0.8791928741724604
2215	1	-1.3723501522612267
2215	2	-1.2899969439969077
2215	3	-1.3300110447455822
2215	4	-1.5169870215317403
2215	5	-1.6405640638713734
2215	6	-1.6757519841105832
2215	7	-1.3630020900338096
2215	8	-1.4168490308672332
2215	9	-1.4502807711965389
2215	10	-1.698484335093653
2215	11	-1.5205128774601135
2215	12	-1.4156403216465705
2215	13	-1.6174327645088482
2215	14	-1.70024366054934
2215	15	-1.5055996648256667
2215	16	-1.67484562998105
2215	17	-1.8207770724762895
2215	18	-1.6271420744750742
2215	19	-2.1144106049468334
2215	20	-2.044214210089301
2215	21	-1.9992286378191295
2215	22	-2.0112773882005825
2215	23	-2.044568916753647
2215	24	-2.301152797082338
2215	25	-2.4319427330092793
2216	1	-1.6039364372784384
2216	2	-1.6225260251627702
2216	3	-1.6840980921903608
2216	4	-1.6528803688066063
2216	5	-1.788114275172724
2216	6	-1.684725974438642
2216	7	-1.5596108122052001
2216	8	-1.548640082145937
2216	9	-1.6947986991362427
2216	10	-1.603392625948878
2216	11	-1.749478154330241
2216	12	-1.8434040126673494
2216	13	-1.7062046026128002
2216	14	-1.5626572883975527
2216	15	-1.8118080036049165
2216	16	-1.848223401949852
2216	17	-2.0206158735044752
2216	18	-2.1799151324631367
2216	19	-1.8774028064019816
2216	20	-1.7599149899437876
2216	21	-1.8073138554377408
2216	22	-2.0622145206015787
2216	23	-2.250376436467846
2216	24	-2.2867015270958433
2216	25	-2.4061918507716626
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19261.0
3	0.0
4	154.0
5	1138.0
6	2314.0
7	2258.0
8	1941.0
9	1485.0
10	1486.0
11	1914.0
12	1959.0
13	1750.0
14	980.0
15	940.0
16	952.0
17	1030.0
18	1190.0
19	1641.0
20	2266.0
21	2826.0
22	3370.0
23	3859.0
24	4292.0
25	4736.0
26	5168.0
27	5479.0
28	6028.0
29	6995.0
30	8494.0
31	10754.0
32	14076.0
33	18348.0
34	24978.0
35	35041.0
36	52691.0
37	96631.0
38	275035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.876107901045714	16.33860798138662	11.940264932160142	23.84501918540752
2	14.75007981210866	21.353870045031677	39.33638036445658	24.559669778403084
3	19.170073353399093	29.006199421130656	27.427464847485254	24.396262377984996
4	12.20406486583632	15.523576927890833	35.46022586867011	36.81213233760274
5	15.800932826541434	36.10074013223867	30.46415099788694	17.634176043332953
6	33.56941441940262	34.715795927079675	15.932664731154839	15.782124922362865
7	28.564297477151833	32.0842147839737	21.248069903984746	18.103417834889715
8	27.27847644117718	32.508848101570585	20.751185765992794	19.46148969125944
9	29.766316218562782	16.575804797570886	17.356409091857415	36.30146989200892
10	16.030366535833604	26.615007397470418	28.764606393596775	28.590019673099203
11	34.65445300835317	24.615471530608126	20.349784108793028	20.38029135224567
12	26.42459299071375	24.71348449630534	26.76575008589416	22.096172427086742
13	29.669151204156734	21.431312913036887	25.718833353222536	23.180702529583844
14	27.42461865909897	20.992292560224453	24.0393111677255	27.543777612951082
15	27.61398857614666	25.382918404272818	23.910266954359415	23.092826065221107
16	25.40620979417844	25.854257826733264	25.81911048197006	22.92042189711824
17	23.641127341910934	27.49427622456548	25.102544258488923	23.762052175034665
18	25.804147547168593	23.142358703818957	29.394797346756484	21.658696402255966
19	25.861040246919153	27.222062755393296	23.77419282012249	23.14270417756506
20	24.41996771910136	26.315220879065947	25.90227691593261	23.36253448590008
21	25.400953968904094	27.593502175294205	23.59476639372019	23.410777462081512
22	23.789730972503925	26.93886500901273	23.011637543249805	26.259766475233544
23	24.16483654692685	24.70677773155449	23.633537468275236	27.494848253243426
24	22.242517257945426	27.33332903429664	23.5307773410673	26.893376366690635
25	24.694156568620496	26.96194449997743	23.703268993892802	24.64062993750927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	491.0
1	491.0
2	639.0
3	787.0
4	787.0
5	787.0
6	1758.5
7	2730.0
8	2730.0
9	2730.0
10	2698.5
11	2667.0
12	2667.0
13	2667.0
14	2848.0
15	3029.0
16	3029.0
17	3029.0
18	4698.5
19	6368.0
20	6368.0
21	6368.0
22	8316.0
23	10264.0
24	10264.0
25	10264.0
26	15236.0
27	20208.0
28	20208.0
29	20208.0
30	24079.5
31	27951.0
32	27951.0
33	27951.0
34	32482.0
35	37013.0
36	37013.0
37	37013.0
38	40504.0
39	43995.0
40	43995.0
41	43995.0
42	49755.0
43	55515.0
44	55515.0
45	55515.0
46	70657.5
47	85800.0
48	85800.0
49	85800.0
50	105317.0
51	124834.0
52	124834.0
53	124834.0
54	93630.0
55	62426.0
56	62426.0
57	62426.0
58	53925.5
59	45425.0
60	45425.0
61	45425.0
62	40598.0
63	35771.0
64	35771.0
65	35771.0
66	29926.0
67	24081.0
68	24081.0
69	24081.0
70	18683.0
71	13285.0
72	13285.0
73	13285.0
74	10994.5
75	8704.0
76	8704.0
77	8704.0
78	7649.0
79	6594.0
80	6594.0
81	6594.0
82	4936.0
83	3278.0
84	3278.0
85	3278.0
86	2423.5
87	1569.0
88	1569.0
89	1569.0
90	1083.0
91	597.0
92	597.0
93	597.0
94	328.0
95	59.0
96	59.0
97	59.0
98	39.0
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17835947775318384
2	0.01940782087062522
3	0.02822955763000032
4	0.042665126872614124
5	0.030956276264716257
6	0.05918583389471658
7	0.06961152279215987
8	0.0713758701440349
9	0.056619510473807456
10	0.04394828858306868
11	0.6311551663298367
12	0.563147595675745
13	0.5386071279633016
14	0.5260963012863695
15	0.5379655471080743
16	0.5155102171751195
17	0.5196804927340968
18	0.5193597023064832
19	0.5353992236871652
20	0.5259359060725628
21	0.5315497385558015
22	0.5174349597408013
23	0.5264170917139832
24	0.5078112469123921
25	0.5145478458922785
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	623460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.72249611867489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.73896053328603	44.62045116810234
2	6.134384118197462	6.100337810150685
3	1.3992173718919891	2.087177410292457
4	0.628642524226584	1.2503070188356122
5	0.38201919790666455	0.9497474042586712
6	0.2450630561158009	0.7311088113929111
7	0.17128471857824207	0.5961692628286474
8	0.12699468788359275	0.5051594300307416
9	0.10685510782577645	0.47817924157149805
>10	0.762718940305604	7.839880101763988
>50	0.13752970323132205	4.903799590205811
>100	0.14270729160101916	14.252346329071464
>500	0.015532766432763991	5.14241672511373
>1k	0.00711918461501683	6.8510313435111865
>5k	6.471986013651663E-4	1.845220119011397
>10k+	3.2359930068258315E-4	1.8466682338588947
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	11477	1.8408558688608732	No Hit
CTTCCGTACTCTGCGTTGATACCAC	6030	0.9671831392551246	No Hit
GAGTACGGAAGCAGTGGTATCAACG	5438	0.8722291726814873	No Hit
TCCGTACTCTGCGTTGATACCACTG	4242	0.6803964969685304	No Hit
GCTTCCGTACTCTGCGTTGATACCA	4214	0.6759054309819396	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3049	0.4890450068969942	No Hit
GTATCAACGCAGAGTACGGAAGCAG	2905	0.4659480961088121	No Hit
GCGTTGATACCACTGCTTCCGTACT	2513	0.40307317229653866	No Hit
CCCATGTACTCTGCGTTGATACCAC	2279	0.36554069226574276	No Hit
GTACATGGAAGCAGTGGTATCAACG	2217	0.35559618900971995	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2015	0.3231963558207423	No Hit
TATCAACGCAGAGTACGGAAGCAGT	1922	0.30827960093670803	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1793	0.28758861835562827	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1786	0.2864658518589805	No Hit
TCCATGTACTCTGCGTTGATACCAC	1651	0.2648124979950598	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1417	0.22728001796426395	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1334	0.2139672152182979	No Hit
GGTATCAACGCAGAGTACGGAAGCA	1318	0.21140089179738875	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1236	0.19824848426522954	No Hit
CAGTGGTATCAACGCAGAGTACGGA	1227	0.19680492734096816	No Hit
GTATCAACGCAGAGTACATGGGAAG	1167	0.18718121451255895	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1097	0.17595354954608153	No Hit
ATACCACTGCTTCCGTACTCTGCGT	1093	0.17531196869085428	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	1086	0.17418920219420653	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1018	0.16328232765534276	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	960	0.1539794052545472	No Hit
GTACGGGAAGCAGTGGTATCAACGC	956	0.15333782439931992	No Hit
GTATCTGATCGTCTTCGAACCTCCG	875	0.1403458120809675	No Hit
CATGTACTCTGCGTTGATACCACTG	858	0.13761909344625156	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	836	0.13409039874250153	No Hit
GTCCTATTCCATTATTCCTAGCTGC	830	0.1331280274596606	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	819	0.1313636801077856	No Hit
CTATTGGAGCTGGAATTACCGCGGC	813	0.13040130882494466	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	793	0.12719340454880826	No Hit
GAATAGGACCGCGGTTCTATTTTGT	790	0.1267122189073878	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	764	0.1225419433484105	No Hit
GTGGTATCAACGCAGAGTACGGAAG	748	0.11997561992750136	No Hit
GTACTCTGCGTTGATACCACTGCTT	739	0.11853206300323998	No Hit
GAGTACATGGAAGCAGTGGTATCAA	722	0.11580534436852405	No Hit
CTTTAATATACGCTATTGGAGCTGG	701	0.11243704487858082	No Hit
GAATAACGCCGCCGCATCGCCAGTC	701	0.11243704487858082	No Hit
TATCAACGCAGAGTACATGGGAAGC	697	0.11179546402335355	No Hit
TATCAACGCAGAGTACGGGAAGCAG	693	0.11115388316812627	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	685	0.10987072145767171	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	673	0.10794597889198987	No Hit
GAACTACGACGGTATCTGATCGTCT	673	0.10794597889198987	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	664	0.10650242196772848	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	650	0.10425688897443301	No Hit
GCCCATGTACTCTGCGTTGATACCA	648	0.10393609854681936	No Hit
GAGTACATGGGAAGCAGTGGTATCA	636	0.10201135598113752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.6039521380682E-4	0.0
12	0.0	0.0	0.0	1.6039521380682E-4	0.0
13	0.0	0.0	0.0	1.6039521380682E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCA	40	0.0052271807	14.269314	6
CGGACAT	60	2.5308156E-5	14.269313	5
AGAGTGT	85	2.6461748E-7	13.429942	6
GTATTAG	150	0.0	13.264489	1
TAGAGTG	75	1.4547872E-5	12.683834	5
CTCGTAG	60	4.0394388E-4	12.683834	10
ACGGTAT	130	1.9281288E-10	12.439914	9
AGGCCCG	185	0.0	12.341028	10
CCGAGCC	195	0.0	12.189106	14
GCCGCCT	195	0.0	12.186156	18
ACAGTCT	55	0.0030339332	12.107295	8
GCTCGTA	55	0.0030339332	12.107295	9
TGTTCAA	95	1.0195963E-6	12.016264	10
TGCGGTA	95	1.0265794E-6	12.009478	15
ATTCCGG	80	2.9392055E-5	11.843293	1
AAATTAG	80	2.9392055E-5	11.843293	1
GTATAAA	80	2.9392055E-5	11.843293	1
CAATACG	80	2.9830295E-5	11.826179	4
CGAGCCG	185	0.0	11.820139	15
GCTCTTA	105	2.805191E-7	11.730499	1
>>END_MODULE