FastQCFastQC Report
Thu 2 Feb 2017
SRR4050270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623460
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA103001.652070702210246No Hit
GAGTACGGAAGCAGTGGTATCAACG56720.909761652712283No Hit
CTTCCGTACTCTGCGTTGATACCAC55310.8871459275655214No Hit
GTATCAACGCAGAGTACTTTTTTTT48510.7780771821768838No Hit
TCCGTACTCTGCGTTGATACCACTG44720.7172873961440991No Hit
GCTTCCGTACTCTGCGTTGATACCA40590.6510441728418824No Hit
TATCAACGCAGAGTACTTTTTTTTT36290.5820742309049498No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30710.4925737016007442No Hit
GTATCAACGCAGAGTACGGAAGCAG29720.47669457543386906No Hit
CCCATGTACTCTGCGTTGATACCAC24430.39184550733006124No Hit
GCGTTGATACCACTGCTTCCGTACT24110.38671286048824305No Hit
TATCAACGCAGAGTACGGAAGCAGT21980.35254867994739036No Hit
GTACATGGGAAGCAGTGGTATCAAC19280.30924197221954897No Hit
GTACATGGAAGCAGTGGTATCAACG19220.30827960093670803No Hit
GGTATCAACGCAGAGTACTTTTTTT18890.302986558881083No Hit
TCCATGTACTCTGCGTTGATACCAC18740.30058063067398066No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15170.24331953934494596No Hit
CAGTGGTATCAACGCAGAGTACGGA14590.23401661694415038No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14430.23145029352324129No Hit
AAGCAGTGGTATCAACGCAGAGTAC14320.22968594617136626No Hit
ACTCTGCGTTGATACCACTGCTTCC14040.2251948801847753No Hit
GGTATCAACGCAGAGTACGGAAGCA13600.2181374907772752No Hit
ACGCAGAGTACGGAAGCAGTGGTAT13510.2166939338530138No Hit
GTATCAACGCAGAGTACGGGAAGCA13290.21316523914926377No Hit
GAAGCAGTGGTATCAACGCAGAGTA11400.1828505437397748No Hit
GTATCAACGCAGAGTACATGGGAAG11310.18140698681551343No Hit
AAAAAGTACTCTGCGTTGATACCAC10960.17579315433227471No Hit
ATACCACTGCTTCCGTACTCTGCGT10810.17338722612517243No Hit
GAGTACGGGAAGCAGTGGTATCAAC10340.16584865107625188No Hit
GTACGGGAAGCAGTGGTATCAACGC10210.16376351329676322No Hit
GTGGTATCAACGCAGAGTACGGAAG9330.14964873448176308No Hit
CATGTACTCTGCGTTGATACCACTG9270.14868636319892214No Hit
TATCAACGCAGAGTACGGGAAGCAG8600.13793988387386522No Hit
CTATTGGAGCTGGAATTACCGCGGC8420.13505277002534244No Hit
ATCAACGCAGAGTACGGAAGCAGTG8330.13360921310108106No Hit
GAATAGGACCGCGGTTCTATTTTGT8080.12959933275591057No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7890.126551823693581No Hit
CTTTAATATACGCTATTGGAGCTGG7680.12318352420363776No Hit
GATTAAGAGGGACGGCCGGGGGCAT7610.12206075770699001No Hit
TATCAACGCAGAGTACATGGGAAGC7440.11933403907227408No Hit
GTATCTGATCGTCTTCGAACCTCCG7350.11789048214801272No Hit
GAATAACGCCGCCGCATCGCCAGTC7160.11484297308568313No Hit
GAACTACGACGGTATCTGATCGTCT7050.11307862573380811No Hit
CTCTTAATCATGGCCTCAGTTCCGA7010.11243704487858082No Hit
GTCCTATTCCATTATTCCTAGCTGC6990.11211625445096719No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6940.11131427838193307No Hit
ATACAGGACTCTTTCGAGGCCCTGT6780.10874795496102396No Hit
ATATTAAAGTTGCTGCAGTTAAAAA6630.10634202675392167No Hit
GATACCACTGCTTCCGTACTCTGCG6580.10554005068488756No Hit
GCCCATGTACTCTGCGTTGATACCA6570.10537965547108075No Hit
GAGTACATGGGAAGCAGTGGTATCA6370.10217175119494434No Hit
ATCAACGCAGAGTACTTTTTTTTTT6320.10136977512591025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGT250.00604504818.9907726
CTTATAC402.7226043E-416.6529983
CCGCACT601.4732632E-615.8231079
AGAGTGT755.860784E-815.1926196
CGAGCCG1200.015.0295415
GATTATA400.005157673314.2981411
CCTTATA400.005213055814.27514652
TTATACT400.00521583814.2739994
CTTATTC400.00521583814.2739993
CCGACCA602.5790601E-514.2407969
GCCGCCT1152.5465852E-1114.03328218
TACTCCC1101.8553692E-1013.8159075
CGGTTCT1101.8735591E-1013.80925712
GGTCCAA1257.2759576E-1213.67116411
ACCCGCA707.288343E-613.563757
CGCACTT707.294386E-613.56266310
TAGGACA500.001478911813.3223984
CGCATCG500.001505101313.291409513
CGGTCCA1153.783498E-1013.20885410
CCGCCTG1251.0186341E-1012.92305819
Produced by FastQC (version 0.11.5)