##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050270_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623460
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.808650113880603	31.0	31.0	34.0	27.0	34.0
2	30.585272511468258	31.0	31.0	34.0	26.0	34.0
3	30.951863792384437	31.0	31.0	34.0	27.0	34.0
4	34.53436467455811	37.0	35.0	37.0	31.0	37.0
5	34.43972829050781	37.0	35.0	37.0	31.0	37.0
6	34.41303692297822	37.0	35.0	37.0	31.0	37.0
7	34.28645622814615	37.0	35.0	37.0	30.0	37.0
8	34.26239694607513	37.0	35.0	37.0	30.0	37.0
9	35.80161036794662	39.0	37.0	39.0	30.0	39.0
10	35.74010521926026	39.0	37.0	39.0	30.0	39.0
11	35.73790299297469	39.0	37.0	39.0	30.0	39.0
12	35.664053828633755	39.0	37.0	39.0	30.0	39.0
13	35.62284829820678	39.0	37.0	39.0	30.0	39.0
14	36.788231803162994	40.0	37.0	41.0	30.0	41.0
15	36.7925255830366	40.0	37.0	41.0	31.0	41.0
16	36.739786032784785	40.0	37.0	41.0	31.0	41.0
17	36.71845186539634	40.0	37.0	41.0	30.0	41.0
18	36.68645622814615	40.0	37.0	41.0	30.0	41.0
19	36.75642382831296	40.0	37.0	41.0	30.0	41.0
20	36.69528919257049	40.0	37.0	41.0	30.0	41.0
21	36.64052866262471	40.0	37.0	41.0	30.0	41.0
22	36.57197414429154	40.0	37.0	41.0	30.0	41.0
23	36.48989028967376	40.0	37.0	41.0	30.0	41.0
24	36.42898983094344	40.0	37.0	41.0	30.0	41.0
25	36.23480094953967	40.0	36.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.46759765747224336
1101	2	0.7191809834675666
1101	3	0.3586406214763116
1101	4	0.4249572174236107
1101	5	0.6527959191766897
1101	6	0.6124489030342275
1101	7	0.4981490276814924
1101	8	0.6512672954860719
1101	9	0.6644304421235532
1101	10	0.6496520702089654
1101	11	0.6268280851313932
1101	12	0.6936271933089344
1101	13	0.8704202229819131
1101	14	0.8274700597961626
1101	15	0.7783420259590272
1101	16	0.7870825153938128
1101	17	0.9758600749029327
1101	18	0.7980211941554956
1101	19	0.8167905612129189
1101	20	0.9054276029236306
1101	21	0.479758032891219
1101	22	0.6965315285559797
1101	23	0.9210412165062394
1101	24	0.9252444692463584
1101	25	0.9085138472519887
1102	1	0.7780657404907423
1102	2	1.062895717778936
1102	3	0.920499705917706
1102	4	0.8569674230072408
1102	5	0.9225005460350388
1102	6	0.7926952108699581
1102	7	0.6971579085018647
1102	8	0.7819699106668807
1102	9	0.7801607792022054
1102	10	0.9444634141076946
1102	11	0.9486130809608468
1102	12	0.9123039020082118
1102	13	0.9717032807317807
1102	14	0.9837160732400534
1102	15	1.056557030129575
1102	16	1.2046380818527709
1102	17	1.1855529260878583
1102	18	1.1040930257633619
1102	19	1.0098748063431842
1102	20	1.1260203118191683
1102	21	1.1814336130884655
1102	22	1.2528830324076097
1102	23	1.2237815138419919
1102	24	1.1071933431975935
1102	25	1.1284466277828713
1103	1	0.514803189091559
1103	2	0.7263857379011682
1103	3	0.4797978024294345
1103	4	0.358957149419858
1103	5	0.5849269624027897
1103	6	0.6613120260578711
1103	7	0.592484801054141
1103	8	0.518792347581801
1103	9	0.7239410094205496
1103	10	0.6590426594614556
1103	11	0.457520606176125
1103	12	0.5927278437162258
1103	13	0.744272052525659
1103	14	0.8231918953145225
1103	15	0.7329665238241887
1103	16	1.0517191082116426
1103	17	1.0398479605206248
1103	18	1.0139391719182669
1103	19	0.8242503297087254
1103	20	1.0734162948714996
1103	21	1.0918634289887805
1103	22	0.9275402670378483
1103	23	0.8503841059855688
1103	24	0.9736267086098493
1103	25	0.6416610607983344
1104	1	0.5065326485500208
1104	2	0.9893394073530146
1104	3	0.6083939908854141
1104	4	0.7026366653579572
1104	5	0.5791730608732166
1104	6	0.7616289772168443
1104	7	0.6737403366129087
1104	8	0.679132925337079
1104	9	0.7391812726883344
1104	10	0.5740053902919513
1104	11	0.7123146002411502
1104	12	0.7797967784375928
1104	13	0.7780292603005066
1104	14	0.8889644961001864
1104	15	0.9918588331084095
1104	16	0.9895323792633945
1104	17	0.9191749351214185
1104	18	0.9942581432867286
1104	19	0.9955530075949426
1104	20	0.9349555713162303
1104	21	0.8703955004302841
1104	22	0.8677125022148644
1104	23	0.7745511083272874
1104	24	0.9229933606176743
1104	25	1.0362769561514824
1105	1	0.4591510756321604
1105	2	0.8112369614829262
1105	3	0.6644821739533491
1105	4	0.5728406310661356
1105	5	0.5661561058955584
1105	6	0.6710103019199352
1105	7	0.7419564362043047
1105	8	0.6682639827319719
1105	9	0.8497752697097596
1105	10	0.8799825927206371
1105	11	0.917997524974794
1105	12	1.0088846846505959
1105	13	0.9619444663187622
1105	14	0.9892444537127432
1105	15	0.9914829309432136
1105	16	1.1736253929724896
1105	17	1.0867055800979344
1105	18	1.1075848893821174
1105	19	1.0238471039022699
1105	20	1.2458648488203323
1105	21	1.2495733844948944
1105	22	1.1778878754916846
1105	23	1.039274539800921
1105	24	1.2575880545497782
1105	25	1.2175787471164625
1106	1	0.048687128292730364
1106	2	0.5362596837479892
1106	3	0.22870470525958098
1106	4	0.27740872441562914
1106	5	0.26122330871587707
1106	6	0.11483925605309508
1106	7	0.1364011866725221
1106	8	0.1523124936202933
1106	9	0.3050055219957599
1106	10	0.22891854806564282
1106	11	0.09414442556602864
1106	12	0.05623421276194307
1106	13	0.035888225269957275
1106	14	0.07337995185823587
1106	15	0.23647829468306725
1106	16	0.07669657104160166
1106	17	0.17863665130354178
1106	18	0.07068071766605755
1106	19	0.13720366443989462
1106	20	0.025173127987457633
1106	21	-0.03471313638204521
1106	22	0.06039999403304819
1106	23	0.016761135959711737
1106	24	-0.08624457186864021
1106	25	-0.021404397094180183
1107	1	0.3506729310293828
1107	2	0.754580402144299
1107	3	0.5561286712882776
1107	4	0.5644816894801465
1107	5	0.3004421568763789
1107	6	0.5599667996174631
1107	7	0.48928683252034233
1107	8	0.39391121432122134
1107	9	0.6281528395998919
1107	10	0.27286757767295455
1107	11	0.4638578262910116
1107	12	0.4444651988099224
1107	13	0.46906265407031356
1107	14	0.6340273954437663
1107	15	0.5816908336953048
1107	16	0.5259462258525787
1107	17	0.25164538937310255
1107	18	0.24418565445699159
1107	19	0.29419999922117057
1107	20	0.3222171005335923
1107	21	0.4033124160043755
1107	22	0.41301016056365825
1107	23	0.07414482153702551
1107	24	0.18037237414077367
1107	25	0.39496915285996437
1108	1	-0.10484865774857255
1108	2	0.3319910496450298
1108	3	0.21966569688260762
1108	4	0.1714119961567846
1108	5	0.15866410447358703
1108	6	0.0795132347318841
1108	7	0.1508704070539295
1108	8	0.18474994078386686
1108	9	0.2541211637730356
1108	10	0.09950801042415236
1108	11	0.08705858078709383
1108	12	0.11219965087882144
1108	13	0.2855802643991012
1108	14	0.34249054329681883
1108	15	0.28409091174662393
1108	16	0.12285886257789258
1108	17	0.27007742510326693
1108	18	0.11476139133732488
1108	19	0.16335563571883682
1108	20	0.2911656842273729
1108	21	0.310427010516932
1108	22	0.08121617268329118
1108	23	0.0511879774120132
1108	24	0.19366386329489416
1108	25	0.007642735740709838
1109	1	-0.15820265970100422
1109	2	0.19769794860122758
1109	3	-0.1498623934871759
1109	4	-0.05279067073873733
1109	5	0.07292437888705194
1109	6	-0.24546611452844758
1109	7	-0.25996774478532103
1109	8	-0.32594553321765574
1109	9	-0.3475362592292015
1109	10	-0.26887007511817984
1109	11	-0.22122638869272038
1109	12	-0.1884268442883652
1109	13	-0.3103764816879817
1109	14	-0.36142183320103527
1109	15	-0.43291671561748046
1109	16	-0.5548329747479173
1109	17	-0.5834610925490935
1109	18	-0.5177627190717189
1109	19	-0.4648881395582052
1109	20	-0.5717091132881009
1109	21	-0.41726858148881263
1109	22	-0.31739137744168033
1109	23	-0.26189118196878525
1109	24	-0.3132511843895429
1109	25	-0.5089722593782042
1110	1	-0.09140177294355212
1110	2	0.2077418340466437
1110	3	0.05593600061144954
1110	4	-0.11439833873468075
1110	5	-0.012670552203964291
1110	6	0.12515153487488817
1110	7	0.06066315427383984
1110	8	0.12458046734680295
1110	9	-0.08919274628224372
1110	10	0.14355616307831554
1110	11	0.23554633985306594
1110	12	0.12892287983871853
1110	13	0.16206315697343143
1110	14	0.05955528044223257
1110	15	0.2006796718584667
1110	16	0.2392555243353769
1110	17	0.193357611225224
1110	18	0.13461155462092478
1110	19	0.10607830972792698
1110	20	0.10679822141975137
1110	21	0.2823315589790809
1110	22	0.23508695452359518
1110	23	0.30199585771339343
1110	24	0.1642664117997228
1110	25	0.26967769764119964
1111	1	-0.07415272875399026
1111	2	0.279894408066383
1111	3	0.14331168174546605
1111	4	0.19716410966425713
1111	5	-0.09285066372576267
1111	6	-0.11084790624591534
1111	7	-0.022667003609527114
1111	8	0.08888840978224977
1111	9	0.021281157869971423
1111	10	0.13212454321777756
1111	11	0.18227499130298241
1111	12	-0.044558302444812625
1111	13	-0.08493604281317602
1111	14	-0.03620993948943152
1111	15	-0.13293310107145118
1111	16	0.15877056961729608
1111	17	-0.04769079533398468
1111	18	0.07759317511654729
1111	19	0.14255787888287585
1111	20	-0.03480779415265545
1111	21	-0.06760937765164243
1111	22	-0.2840991141423359
1111	23	-0.33793352027947066
1111	24	-0.07034853313797385
1111	25	-0.10815639144922073
1112	1	-0.49391297980258386
1112	2	-0.29946102098229943
1112	3	-0.4220450860652889
1112	4	-0.33517190733433466
1112	5	-0.3398921837734221
1112	6	-0.45514926281721557
1112	7	-0.4522161846924533
1112	8	-0.5906285304708874
1112	9	-0.5402742625811499
1112	10	-0.5558886233229927
1112	11	-0.5314939924046556
1112	12	-0.7024861689616344
1112	13	-0.5959992532628888
1112	14	-0.8086234573017208
1112	15	-0.5878604210294469
1112	16	-0.629827844747993
1112	17	-0.631016452279269
1112	18	-0.608982971582293
1112	19	-0.7151310984380004
1112	20	-0.856264219903565
1112	21	-0.9017686903385567
1112	22	-0.839838903010957
1112	23	-0.7051013015576757
1112	24	-0.5949897387607734
1112	25	-0.7883150777428796
1113	1	-0.5879149025173795
1113	2	-0.1796451655228779
1113	3	-0.34505819818731354
1113	4	-0.1979663849802975
1113	5	-0.29668957522427775
1113	6	-0.13817585376459363
1113	7	-0.33288992774243553
1113	8	-0.48630242218438724
1113	9	-0.4791339143120936
1113	10	-0.45346739020869364
1113	11	-0.21134909503203403
1113	12	-0.2833908360049193
1113	13	-0.3786026487375693
1113	14	-0.5385444674207491
1113	15	-0.7508075265508154
1113	16	-0.5141626746273715
1113	17	-0.49713702830084117
1113	18	-0.4622213584840509
1113	19	-0.2789226127245641
1113	20	-0.4326068992165375
1113	21	-0.4069966906160616
1113	22	-0.34095387937345834
1113	23	-0.35653887774773096
1113	24	-0.48358427388996006
1113	25	-0.3886520483768763
1114	1	-0.7254130626406265
1114	2	-0.9532009849303904
1114	3	-0.6526453592820154
1114	4	-0.36609579455158325
1114	5	-0.44915010373500763
1114	6	-0.6165663786446345
1114	7	-0.46868656984469936
1114	8	-0.5677711835302901
1114	9	-0.5025665846356162
1114	10	-0.818844344157803
1114	11	-0.834182210851047
1114	12	-0.6685315007155879
1114	13	-0.6261185698044045
1114	14	-0.8256702364892305
1114	15	-0.8280411369286966
1114	16	-0.885596993480469
1114	17	-0.855704966363227
1114	18	-0.9406982349130217
1114	19	-0.9424753902025103
1114	20	-0.9873626126471464
1114	21	-0.8170586086017195
1114	22	-0.900678731938001
1114	23	-0.9549904374725813
1114	24	-0.9732044252014234
1114	25	-0.8743952113948623
1115	1	-0.6813975517531752
1115	2	-1.0792991114999992
1115	3	-0.6585446924678102
1115	4	-0.469955234350671
1115	5	-0.38149203605288307
1115	6	-0.5392236284449794
1115	7	-0.6470877552921763
1115	8	-0.6328659134849701
1115	9	-0.6394530530220806
1115	10	-0.5910334769144541
1115	11	-0.6533177579177405
1115	12	-0.703644608955031
1115	13	-0.9360091660883327
1115	14	-0.8053425064479569
1115	15	-0.6512208688559156
1115	16	-0.9893621363211196
1115	17	-0.9302428631561668
1115	18	-0.5800239154387725
1115	19	-0.7221313025255824
1115	20	-0.9141891351100284
1115	21	-0.9642301809553899
1115	22	-0.8397426902933844
1115	23	-0.90532288236178
1115	24	-0.8976282996517639
1115	25	-0.7204025049759366
1116	1	-0.7279722727403808
1116	2	-1.0169635702605504
1116	3	-0.6755939620440721
1116	4	-0.37595287521810405
1116	5	-0.2322406069629679
1116	6	-0.2458745675612093
1116	7	-0.5318350559001104
1116	8	-0.454415682108511
1116	9	-0.5243546046054206
1116	10	-0.5591488929384028
1116	11	-0.5091838317767383
1116	12	-0.6269274326552363
1116	13	-0.4154121240776263
1116	14	-0.6903257203123943
1116	15	-0.5753656833010652
1116	16	-0.6434770079298673
1116	17	-0.906760420546604
1116	18	-0.6743801444561015
1116	19	-0.9167869599069434
1116	20	-0.638297655208028
1116	21	-0.661998078605329
1116	22	-0.7729701651141809
1116	23	-0.7862048349976618
1116	24	-0.9596553389403937
1116	25	-0.999034208088176
1201	1	0.38530965535957407
1201	2	0.7301852559641944
1201	3	0.40995538092437656
1201	4	0.21026661974565997
1201	5	0.2832839080851741
1201	6	0.401276277193638
1201	7	0.3997448476141514
1201	8	0.3549866311269909
1201	9	0.36205463218834666
1201	10	0.5401728809458533
1201	11	0.39465137542537576
1201	12	0.33541890674041497
1201	13	0.4362360057542247
1201	14	0.46617293736508714
1201	15	0.3874085844771358
1201	16	0.2619851345379942
1201	17	0.45342003604490344
1201	18	0.38790160197614654
1201	19	0.33654260550352433
1201	20	0.29646950734420585
1201	21	0.32064054394972175
1201	22	0.23567721786153584
1201	23	0.3352965476954637
1201	24	0.3952002352304973
1201	25	0.3039532164692602
1202	1	0.3733251911232216
1202	2	0.6310182652453591
1202	3	0.3162441793389448
1202	4	0.2472109093218009
1202	5	0.35268651205907275
1202	6	0.3214212339268201
1202	7	0.2347288948773567
1202	8	0.3013610730154568
1202	9	0.3401354312775666
1202	10	0.21404834184173893
1202	11	0.36392420236278156
1202	12	0.344147232335537
1202	13	0.44956040617963566
1202	14	0.32428493916415846
1202	15	0.34601814300096123
1202	16	0.4073166750429493
1202	17	0.42372592567351575
1202	18	0.6691031405602175
1202	19	0.5083027475554971
1202	20	0.6857561084508248
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2210	16	0.2540740391538918
2210	17	0.41779259652977885
2210	18	0.28498292238359824
2210	19	0.42664904334213816
2210	20	0.4787451151057738
2210	21	0.5732507846809156
2210	22	0.6322115690395123
2210	23	0.5979680015116244
2210	24	0.37831323454219756
2210	25	0.4324931430653436
2211	1	-0.527893228708205
2211	2	-1.0598333335934171
2211	3	-0.8065280765184291
2211	4	-0.8349941176043032
2211	5	-0.517747651181125
2211	6	-0.6650218219435047
2211	7	-0.5429337679473463
2211	8	-0.5718844132193084
2211	9	-0.7191516474286885
2211	10	-0.36722533316716977
2211	11	-0.6354218008100787
2211	12	-0.6316840235326282
2211	13	-0.710660182849189
2211	14	-0.9181957161277126
2211	15	-0.7793085856119433
2211	16	-0.9956414195167866
2211	17	-0.6155203591712421
2211	18	-0.6332818973737631
2211	19	-0.6138716300143514
2211	20	-0.343793540268706
2211	21	-0.5510388560266364
2211	22	-0.5716124676203407
2211	23	-0.48707431935159207
2211	24	-0.7294884165430346
2211	25	-0.6310144935766218
2212	1	-0.23383724499939618
2212	2	-1.2406142654061085
2212	3	-0.511911959876663
2212	4	-0.24617508097378504
2212	5	-0.21029975685691227
2212	6	-0.03182901491817347
2212	7	0.07442542222665338
2212	8	-0.13566018253921186
2212	9	-0.0446874964294679
2212	10	-0.21824969599130384
2212	11	-0.26133781045462
2212	12	-0.15416083669180125
2212	13	-0.22076673630589028
2212	14	-0.29544228802540573
2212	15	-0.22447324792968715
2212	16	0.024214701266807026
2212	17	-1.3125929742918672
2212	18	-0.68499952331738
2212	19	-0.2388083931757521
2212	20	-0.4759651894558701
2212	21	-0.15402590295256147
2212	22	-0.2673784346139172
2212	23	-0.276100447383385
2212	24	-0.05942840934414306
2212	25	-0.1875765790856505
2213	1	-0.12202085646537597
2213	2	-0.5471798380652793
2213	3	-0.17458375189875497
2213	4	-0.006259179577675411
2213	5	0.0793362932585211
2213	6	6.028198770735571E-5
2213	7	-0.020243749829845115
2213	8	-0.010719141312947045
2213	9	-0.16697610893617565
2213	10	-0.03571237771322444
2213	11	-0.1523113415457047
2213	12	-0.020390778773759166
2213	13	0.04222567686955614
2213	14	-0.13789203223503677
2213	15	-0.22190197703632464
2213	16	-0.10687714838689288
2213	17	-0.9304112227723067
2213	18	-0.7002476466619996
2213	19	-0.5316267848758827
2213	20	-0.32571054484121476
2213	21	-0.2633846415498837
2213	22	-0.23368361477474764
2213	23	-0.10754709995723033
2213	24	0.015210056410118966
2213	25	0.05471581442096607
2214	1	-1.0103741049593609
2214	2	-2.479017059233989
2214	3	-1.1487035627237674
2214	4	-0.9358269101632501
2214	5	-1.0470202604667165
2214	6	-1.086405024507755
2214	7	-1.1757381256056973
2214	8	-1.0254289435321695
2214	9	-1.3187711389041183
2214	10	-1.2357493140532512
2214	11	-1.0810356289028107
2214	12	-1.1715194314731576
2214	13	-1.1320738654244593
2214	14	-1.1922798409581077
2214	15	-1.2618179754217849
2214	16	-1.4088717107483788
2214	17	-1.2574234917297886
2214	18	-1.3569186543005856
2214	19	-1.2650397026944304
2214	20	-1.4242106248064914
2214	21	-1.2523475300478353
2214	22	-1.29399266151254
2214	23	-1.2550811232766321
2214	24	-1.2206855927174942
2214	25	-1.16905748022441
2215	1	-0.9075094333990386
2215	2	-2.3180138595351387
2215	3	-1.395326939347143
2215	4	-0.90396355612598
2215	5	-0.907825831707072
2215	6	-1.1992791069470599
2215	7	-0.9159828084590771
2215	8	-0.9367031765455351
2215	9	-1.1584267007335924
2215	10	-0.9766922841266563
2215	11	-0.932735233645893
2215	12	-1.1564601429356074
2215	13	-0.9569430542066897
2215	14	-1.1838815725598337
2215	15	-0.9420946552636806
2215	16	-0.9597920947122418
2215	17	-1.1535160947789151
2215	18	-0.9400054226047487
2215	19	-1.0284558517627573
2215	20	-1.1879015715409125
2215	21	-1.0449976335675046
2215	22	-1.2195300227020738
2215	23	-1.204629401085775
2215	24	-1.2511373149083482
2215	25	-1.426181515609315
2216	1	-0.7104598102560935
2216	2	-1.8109542159155652
2216	3	-1.1744250964507899
2216	4	-0.7813756591190568
2216	5	-0.613147140468449
2216	6	-0.5500959936285383
2216	7	-0.5391466650355596
2216	8	-0.5688601930575388
2216	9	-0.8209309485443299
2216	10	-0.9335920357931045
2216	11	-0.8898441083028032
2216	12	-1.0140630706574782
2216	13	-0.869256041615742
2216	14	-1.1452034041286936
2216	15	-0.9950132279992729
2216	16	-1.1962213196501565
2216	17	-1.0076684931582136
2216	18	-1.1584674972790836
2216	19	-1.0716449773672494
2216	20	-1.0317590193690833
2216	21	-1.0387849380592868
2216	22	-0.9161805694715639
2216	23	-0.9800313093330644
2216	24	-1.152185543898021
2216	25	-0.9810283765860959
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9668.0
3	0.0
4	141.0
5	1353.0
6	2298.0
7	1562.0
8	1118.0
9	900.0
10	862.0
11	820.0
12	800.0
13	656.0
14	654.0
15	648.0
16	668.0
17	661.0
18	953.0
19	1393.0
20	1834.0
21	2214.0
22	2554.0
23	3003.0
24	3602.0
25	3852.0
26	4256.0
27	4905.0
28	5423.0
29	6607.0
30	8369.0
31	10945.0
32	14600.0
33	19510.0
34	26918.0
35	37924.0
36	57367.0
37	104610.0
38	279812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51325104520491	17.34767002053454	12.834409334700162	24.304669599560388
2	14.534992837652933	22.58200606674056	37.89820002919478	24.98480106641172
3	19.393866487024027	28.33237096205049	27.678600070573893	24.595162480351586
4	12.720403022670027	16.403800185061428	34.949622166246854	35.9261746260217
5	15.51713900091637	36.52511350305162	30.600165941541718	17.35758155449029
6	33.35585625348075	35.46685840573034	15.459283000759145	15.718002340029754
7	27.550462057378876	32.251588999199285	21.663342378179404	18.534606565242438
8	27.17252665270859	32.461515012955346	20.90807723469625	19.457881099639817
9	28.57724979344975	16.578565297953748	17.758187812332054	37.08599709626445
10	15.710852805200053	27.03656529425164	29.174273073480293	28.078308827068003
11	34.891182632857294	23.99211984509207	20.762411404406595	20.354286117644037
12	26.266411694495424	24.748289846888355	27.01175609943915	21.973542359177074
13	30.04623064633074	20.827371365873375	26.061444090112868	23.06495389768301
14	27.09592244970636	20.624682180789204	24.87900813135939	27.40038723814504
15	27.42371918973844	25.05866520812315	24.498879626852386	23.018735975286027
16	25.441369653400315	25.17110241764869	26.14903674190842	23.23849118704257
17	23.95550636768999	26.53931286690405	26.149873287781094	23.35530747762487
18	25.71680621050268	22.289481281878547	30.422801783594778	21.570910724023996
19	25.85859557950791	26.674044846501783	24.81522471369454	22.65213486029577
20	24.07596317325891	25.31678054726847	27.337279055592983	23.26997722387964
21	25.12371056299735	26.70303529981666	24.940209902940342	23.23304423424565
22	23.638819334275464	26.015261200075066	24.35407780741758	25.99184165823189
23	23.796264365511544	23.638273812865403	25.26774185787266	27.29771996375039
24	22.306163595855967	26.183934262676615	25.092749871280983	26.417152270186428
25	24.647704135702956	25.420699710327376	25.25707642604356	24.67451972792611
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	714.0
1	714.0
2	862.5
3	1011.0
4	1011.0
5	1011.0
6	2361.5
7	3712.0
8	3712.0
9	3712.0
10	3270.5
11	2829.0
12	2829.0
13	2829.0
14	2536.5
15	2244.0
16	2244.0
17	2244.0
18	3717.0
19	5190.0
20	5190.0
21	5190.0
22	7901.5
23	10613.0
24	10613.0
25	10613.0
26	16476.0
27	22339.0
28	22339.0
29	22339.0
30	25918.0
31	29497.0
32	29497.0
33	29497.0
34	33379.5
35	37262.0
36	37262.0
37	37262.0
38	40265.0
39	43268.0
40	43268.0
41	43268.0
42	48710.5
43	54153.0
44	54153.0
45	54153.0
46	69392.0
47	84631.0
48	84631.0
49	84631.0
50	104358.0
51	124085.0
52	124085.0
53	124085.0
54	93647.0
55	63209.0
56	63209.0
57	63209.0
58	54300.0
59	45391.0
60	45391.0
61	45391.0
62	40602.0
63	35813.0
64	35813.0
65	35813.0
66	29864.5
67	23916.0
68	23916.0
69	23916.0
70	18507.0
71	13098.0
72	13098.0
73	13098.0
74	10898.5
75	8699.0
76	8699.0
77	8699.0
78	7643.0
79	6587.0
80	6587.0
81	6587.0
82	4873.0
83	3159.0
84	3159.0
85	3159.0
86	2300.5
87	1442.0
88	1442.0
89	1442.0
90	987.5
91	533.0
92	533.0
93	533.0
94	294.0
95	55.0
96	55.0
97	55.0
98	32.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1754723639046611
2	0.00978410804221602
3	0.0
4	0.15462098610977448
5	0.05597792961858018
6	0.06271452859846663
7	0.043146312514034585
8	0.0264652102781253
9	0.0200494017258525
10	0.013152407532159242
11	0.01956821608443204
12	0.01988900651204568
13	0.010265293683636479
14	0.01138806018028422
15	0.0011227664966477402
16	4.8118564142046E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.00465146120039778
22	0.00785936547653418
23	8.019760690341E-4
24	0.00144355692426138
25	0.11035190709909215
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	623460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.64341627434913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.62488992778239	43.5966082929984
2	6.247596489776172	6.078088735326895
3	1.3942494004575245	2.034631619301512
4	0.6430294638965612	1.2511659955596792
5	0.3762407538973818	0.9150817805602643
6	0.24585307020134708	0.7175479941678543
7	0.16920777262913145	0.5761590884597981
8	0.1290657286929592	0.5022558374051056
9	0.10037863739653836	0.4394483859538588
>10	0.7623618130033324	7.518577899599848
>50	0.1379749547453773	4.782157209824171
>100	0.14295001298368235	14.032673300726131
>500	0.016251857624594978	5.1295133818124885
>1k	0.0089551052217156	8.95688477128024
>5k	6.63341127534489E-4	1.807446009198129
>10k+	3.316705637672445E-4	1.6617596978256475
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	10300	1.652070702210246	No Hit
GAGTACGGAAGCAGTGGTATCAACG	5672	0.909761652712283	No Hit
CTTCCGTACTCTGCGTTGATACCAC	5531	0.8871459275655214	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4851	0.7780771821768838	No Hit
TCCGTACTCTGCGTTGATACCACTG	4472	0.7172873961440991	No Hit
GCTTCCGTACTCTGCGTTGATACCA	4059	0.6510441728418824	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3629	0.5820742309049498	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3071	0.4925737016007442	No Hit
GTATCAACGCAGAGTACGGAAGCAG	2972	0.47669457543386906	No Hit
CCCATGTACTCTGCGTTGATACCAC	2443	0.39184550733006124	No Hit
GCGTTGATACCACTGCTTCCGTACT	2411	0.38671286048824305	No Hit
TATCAACGCAGAGTACGGAAGCAGT	2198	0.35254867994739036	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1928	0.30924197221954897	No Hit
GTACATGGAAGCAGTGGTATCAACG	1922	0.30827960093670803	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1889	0.302986558881083	No Hit
TCCATGTACTCTGCGTTGATACCAC	1874	0.30058063067398066	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1517	0.24331953934494596	No Hit
CAGTGGTATCAACGCAGAGTACGGA	1459	0.23401661694415038	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1443	0.23145029352324129	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1432	0.22968594617136626	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1404	0.2251948801847753	No Hit
GGTATCAACGCAGAGTACGGAAGCA	1360	0.2181374907772752	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	1351	0.2166939338530138	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1329	0.21316523914926377	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1140	0.1828505437397748	No Hit
GTATCAACGCAGAGTACATGGGAAG	1131	0.18140698681551343	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1096	0.17579315433227471	No Hit
ATACCACTGCTTCCGTACTCTGCGT	1081	0.17338722612517243	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1034	0.16584865107625188	No Hit
GTACGGGAAGCAGTGGTATCAACGC	1021	0.16376351329676322	No Hit
GTGGTATCAACGCAGAGTACGGAAG	933	0.14964873448176308	No Hit
CATGTACTCTGCGTTGATACCACTG	927	0.14868636319892214	No Hit
TATCAACGCAGAGTACGGGAAGCAG	860	0.13793988387386522	No Hit
CTATTGGAGCTGGAATTACCGCGGC	842	0.13505277002534244	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	833	0.13360921310108106	No Hit
GAATAGGACCGCGGTTCTATTTTGT	808	0.12959933275591057	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	789	0.126551823693581	No Hit
CTTTAATATACGCTATTGGAGCTGG	768	0.12318352420363776	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	761	0.12206075770699001	No Hit
TATCAACGCAGAGTACATGGGAAGC	744	0.11933403907227408	No Hit
GTATCTGATCGTCTTCGAACCTCCG	735	0.11789048214801272	No Hit
GAATAACGCCGCCGCATCGCCAGTC	716	0.11484297308568313	No Hit
GAACTACGACGGTATCTGATCGTCT	705	0.11307862573380811	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	701	0.11243704487858082	No Hit
GTCCTATTCCATTATTCCTAGCTGC	699	0.11211625445096719	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	694	0.11131427838193307	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	678	0.10874795496102396	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	663	0.10634202675392167	No Hit
GATACCACTGCTTCCGTACTCTGCG	658	0.10554005068488756	No Hit
GCCCATGTACTCTGCGTTGATACCA	657	0.10537965547108075	No Hit
GAGTACATGGGAAGCAGTGGTATCA	637	0.10217175119494434	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	632	0.10136977512591025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.6039521380682E-4	0.0
12	0.0	0.0	0.0	1.6039521380682E-4	0.0
13	0.0	0.0	0.0	1.6039521380682E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCGT	25	0.006045048	18.990772	6
CTTATAC	40	2.7226043E-4	16.652998	3
CCGCACT	60	1.4732632E-6	15.823107	9
AGAGTGT	75	5.860784E-8	15.192619	6
CGAGCCG	120	0.0	15.02954	15
GATTATA	40	0.0051576733	14.298141	1
CCTTATA	40	0.0052130558	14.2751465	2
TTATACT	40	0.005215838	14.273999	4
CTTATTC	40	0.005215838	14.273999	3
CCGACCA	60	2.5790601E-5	14.240796	9
GCCGCCT	115	2.5465852E-11	14.033282	18
TACTCCC	110	1.8553692E-10	13.815907	5
CGGTTCT	110	1.8735591E-10	13.809257	12
GGTCCAA	125	7.2759576E-12	13.671164	11
ACCCGCA	70	7.288343E-6	13.56375	7
CGCACTT	70	7.294386E-6	13.562663	10
TAGGACA	50	0.0014789118	13.322398	4
CGCATCG	50	0.0015051013	13.2914095	13
CGGTCCA	115	3.783498E-10	13.208854	10
CCGCCTG	125	1.0186341E-10	12.923058	19
>>END_MODULE