FastQCFastQC Report
Thu 2 Feb 2017
SRR4050269_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050269_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146234
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT161581.409659807683248No Hit
TATCAACGCAGAGTACTTTTTTTTT103170.9000779945456163No Hit
GGTATCAACGCAGAGTACTTTTTTT80050.6983739794841193No Hit
GTACTTTTTTTTTTTTTTTTTTTTT75200.6560615022761496No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55300.48244948239190255No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50580.4412711540575484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43000.37514155050364933No Hit
GCAGAGTACTTTTTTTTTTTTTTTT27410.2391309278908146No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN25000.21810555261840078No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN20480.1786720687049939No Hit
ATCAACGCAGAGTACTTTTTTTTTT16010.13967479589682386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA903.637797E-813.72149310
GTCTATA500.001498229113.3010461
CGCTACA500.001498725413.3004622
GCGCTAC604.0878166E-412.6676631
CGCCAGT1352.7284841E-1112.66710618
GGTCCAA1051.9914296E-812.6665511
CGCAGAT1457.2759576E-1212.448168
TTAGAGT1405.2750693E-1112.2147084
CGAACGA550.003068800812.09026516
GTCCTTA802.8624772E-511.8759331
GGTATCA33000.011.8615381
TAGAGTG1302.6102498E-911.6927135
AGATTAC1406.7848305E-1011.53560711
TCGCCAG1501.7644197E-1011.40039417
GCATCGC1451.2169039E-911.13733914
ATAGTAC600.00587250311.0837173
ACGATCA600.00587442411.0832319
TTACGGG600.005876345611.08274414
CATCGCC1553.1286618E-1011.03167115
GTATCAA78350.010.9861861