Basic Statistics
Measure | Value |
---|---|
Filename | SRR4050269_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1146234 |
Sequences flagged as poor quality | 0 |
Sequence length | 25 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATCAACGCAGAGTACTTTTTTTT | 16158 | 1.409659807683248 | No Hit |
TATCAACGCAGAGTACTTTTTTTTT | 10317 | 0.9000779945456163 | No Hit |
GGTATCAACGCAGAGTACTTTTTTT | 8005 | 0.6983739794841193 | No Hit |
GTACTTTTTTTTTTTTTTTTTTTTT | 7520 | 0.6560615022761496 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTT | 5530 | 0.48244948239190255 | No Hit |
GAGTACTTTTTTTTTTTTTTTTTTT | 5058 | 0.4412711540575484 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAA | 4300 | 0.37514155050364933 | No Hit |
GCAGAGTACTTTTTTTTTTTTTTTT | 2741 | 0.2391309278908146 | No Hit |
NNNNNNNNNNNNNNNNNNNNNNNNN | 2500 | 0.21810555261840078 | No Hit |
GNNNNNNNNNNNNNNNNNNNNNNNN | 2048 | 0.1786720687049939 | No Hit |
ATCAACGCAGAGTACTTTTTTTTTT | 1601 | 0.13967479589682386 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGGTCCA | 90 | 3.637797E-8 | 13.721493 | 10 |
GTCTATA | 50 | 0.0014982291 | 13.301046 | 1 |
CGCTACA | 50 | 0.0014987254 | 13.300462 | 2 |
GCGCTAC | 60 | 4.0878166E-4 | 12.667663 | 1 |
CGCCAGT | 135 | 2.7284841E-11 | 12.667106 | 18 |
GGTCCAA | 105 | 1.9914296E-8 | 12.66655 | 11 |
CGCAGAT | 145 | 7.2759576E-12 | 12.44816 | 8 |
TTAGAGT | 140 | 5.2750693E-11 | 12.214708 | 4 |
CGAACGA | 55 | 0.0030688008 | 12.090265 | 16 |
GTCCTTA | 80 | 2.8624772E-5 | 11.875933 | 1 |
GGTATCA | 3300 | 0.0 | 11.861538 | 1 |
TAGAGTG | 130 | 2.6102498E-9 | 11.692713 | 5 |
AGATTAC | 140 | 6.7848305E-10 | 11.535607 | 11 |
TCGCCAG | 150 | 1.7644197E-10 | 11.400394 | 17 |
GCATCGC | 145 | 1.2169039E-9 | 11.137339 | 14 |
ATAGTAC | 60 | 0.005872503 | 11.083717 | 3 |
ACGATCA | 60 | 0.005874424 | 11.083231 | 9 |
TTACGGG | 60 | 0.0058763456 | 11.082744 | 14 |
CATCGCC | 155 | 3.1286618E-10 | 11.031671 | 15 |
GTATCAA | 7835 | 0.0 | 10.986186 | 1 |