FastQCFastQC Report
Thu 2 Feb 2017
SRR4050269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146234
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT232612.029341303782648No Hit
TATCAACGCAGAGTACTTTTTTTTT179151.56294439006346No Hit
GTACTTTTTTTTTTTTTTTTTTTTT130521.1386854691101467No Hit
ACGCAGAGTACTTTTTTTTTTTTTT114550.9993596420975124No Hit
GGTATCAACGCAGAGTACTTTTTTT112320.979904626803951No Hit
GAGTACTTTTTTTTTTTTTTTTTTT85300.7441761455339835No Hit
GCAGAGTACTTTTTTTTTTTTTTTT56710.4947506355595804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44770.3905834236290321No Hit
ATCAACGCAGAGTACTTTTTTTTTT33190.2895569316561889No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT30570.2666994697417805No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT19940.17396098876843646No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT18040.157384966769438No Hit
CAACGCAGAGTACTTTTTTTTTTTT16330.1424665469703394No Hit
AACGCAGAGTACTTTTTTTTTTTTT14500.12650122051867244No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA13720.11969632727697835No Hit
TCAACGCAGAGTACTTTTTTTTTTT12600.109925198519674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.014.850031
AACCGCG602.5743377E-514.2457467
CGTCAAT802.0077514E-613.05689219
CCGTCAA853.961659E-612.28830318
TCCGTCA853.961659E-612.28830317
GCGTTAT550.00298811412.1333571
GATCTAT550.00298811412.1333571
TATTAGG550.003061429612.0941592
CTATCCT907.436998E-611.6137264
CTCTTAC752.0065061E-411.4400221
GTATCAA63250.011.3194431
ACAGGAC2200.011.2293113
CCTAATA600.00586232111.0863122
CCTACAC600.00586613211.0853443
AAGGCGT600.00588904511.0795416
GCGCGAC600.005892871411.07857419
TACGCTA1303.2714524E-810.9592229
GTATTAA1053.3205743E-610.895261
ACGCTAT1252.2436325E-710.63775210
CGCGGTC909.529544E-510.55332510
Produced by FastQC (version 0.11.5)