##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050269_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1146234
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.018786739880337	31.0	30.0	34.0	26.0	34.0
2	29.925162750363363	31.0	30.0	34.0	25.0	34.0
3	30.1434044008466	31.0	30.0	34.0	26.0	34.0
4	33.67787729207125	37.0	35.0	37.0	28.0	37.0
5	33.568502591966386	37.0	35.0	37.0	28.0	37.0
6	33.48578039039149	37.0	35.0	37.0	28.0	37.0
7	33.32895639110339	37.0	35.0	37.0	27.0	37.0
8	33.248362027299834	37.0	35.0	37.0	27.0	37.0
9	34.68561044254489	39.0	35.0	39.0	27.0	39.0
10	34.620117707204635	39.0	35.0	39.0	27.0	39.0
11	34.575700947625	39.0	35.0	39.0	27.0	39.0
12	34.55000985837098	39.0	35.0	39.0	27.0	39.0
13	34.460176543358514	39.0	35.0	39.0	26.0	39.0
14	35.50100328554205	40.0	36.0	41.0	25.0	41.0
15	35.47687906657803	40.0	36.0	41.0	26.0	41.0
16	35.48273650929915	40.0	36.0	41.0	26.0	41.0
17	35.5115369113113	40.0	36.0	41.0	26.0	41.0
18	35.47600664436756	40.0	36.0	41.0	26.0	41.0
19	35.52300577369019	40.0	36.0	41.0	26.0	41.0
20	35.46453690956646	40.0	36.0	41.0	26.0	41.0
21	35.400627620538216	40.0	36.0	41.0	26.0	41.0
22	35.31153760924907	40.0	35.0	41.0	26.0	41.0
23	35.1600135748896	40.0	35.0	41.0	25.0	41.0
24	35.08909088371136	40.0	35.0	41.0	25.0	41.0
25	34.882185487431016	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8376888038484047
1101	2	1.0562490849794592
1101	3	1.029363233281238
1101	4	0.8828990514257455
1101	5	0.8896639264326822
1101	6	1.0460790367912693
1101	7	0.9985068042752445
1101	8	0.7901480711804254
1101	9	0.9096418521151648
1101	10	1.0647569442728084
1101	11	1.0568996608950485
1101	12	1.0839760764606439
1101	13	1.136444424275588
1101	14	1.2920633769132763
1101	15	1.3826237747889039
1101	16	1.3037960379534113
1101	17	1.3130334605469116
1101	18	1.3714475962012003
1101	19	1.3331984186033168
1101	20	1.4706195379172655
1101	21	1.4378930405694845
1101	22	1.4412074419577792
1101	23	1.5949394573111988
1101	24	1.462803695553177
1101	25	1.4197420876175002
1102	1	0.8156480647859006
1102	2	0.7953980081135015
1102	3	0.8392867783816698
1102	4	0.7124358548052854
1102	5	0.845953726212386
1102	6	0.8125511788315833
1102	7	0.8834454039631368
1102	8	0.885661598361672
1102	9	0.9800049591625424
1102	10	0.9527041752575087
1102	11	0.9559422485740185
1102	12	0.9714034269309835
1102	13	0.853612963868585
1102	14	0.9607938671052736
1102	15	1.224847583121715
1102	16	1.2291911265685727
1102	17	0.9412434483797938
1102	18	1.1971129586704023
1102	19	1.1955453064760206
1102	20	1.161508277543085
1102	21	1.2858012416503044
1102	22	1.2694603187899887
1102	23	1.27902311189289
1102	24	1.2133008284372906
1102	25	1.233473060196431
1103	1	0.7735584599665053
1103	2	1.0789828038305522
1103	3	0.9471615784958836
1103	4	1.0169017974543735
1103	5	0.8977829853138886
1103	6	1.0103262395456127
1103	7	0.8635337004000547
1103	8	0.8386588838341069
1103	9	1.1317639756103475
1103	10	0.9976646416928432
1103	11	0.9671616929119864
1103	12	1.0123625169376211
1103	13	1.0351404810297993
1103	14	1.1040135129978879
1103	15	1.2090345879293096
1103	16	1.143290089035915
1103	17	1.1684532387672704
1103	18	1.2053810056705316
1103	19	1.1792155045120651
1103	20	1.1934697616570915
1103	21	1.1727428696174798
1103	22	1.050142167804033
1103	23	1.1991958459428318
1103	24	1.2434616716247646
1103	25	1.1952496007005138
1104	1	0.41796210748103135
1104	2	0.5489799794277097
1104	3	0.5837480859200284
1104	4	0.4710407580741318
1104	5	0.5197491731770327
1104	6	0.6252475597521965
1104	7	0.6447058275788748
1104	8	0.8026425022976298
1104	9	0.7721242887155739
1104	10	0.9024544254242883
1104	11	0.834770634164407
1104	12	0.8934410353365863
1104	13	0.7879604455230194
1104	14	0.892738496858442
1104	15	0.8424609920610067
1104	16	0.9079769838067335
1104	17	0.7077027545839272
1104	18	0.712632054734442
1104	19	0.7152730416327913
1104	20	0.802300055394987
1104	21	0.7626278760732532
1104	22	0.9099840012449576
1104	23	1.0597244127601329
1104	24	0.9020587503852582
1104	25	0.926185285012977
1105	1	0.3008052447359333
1105	2	0.4269334108002596
1105	3	0.30085592905728475
1105	4	0.2527564443486412
1105	5	0.289736918275068
1105	6	0.2921715793600441
1105	7	0.2679533765984843
1105	8	0.050032208179985105
1105	9	0.09221007302929962
1105	10	0.2588392293458526
1105	11	0.3149422027918618
1105	12	0.3243846627875655
1105	13	0.29701681807203784
1105	14	0.26254241842706705
1105	15	0.41885805088453765
1105	16	0.3699867877283012
1105	17	0.43902628399569466
1105	18	0.42428646649914725
1105	19	0.3992803945739638
1105	20	0.3767485848067551
1105	21	0.23224797411246811
1105	22	0.238194785558683
1105	23	0.4045038245248378
1105	24	0.347732769993101
1105	25	0.3728887163855248
1106	1	0.5542703860225728
1106	2	0.7913750033368565
1106	3	0.555904391957089
1106	4	0.5281141292636136
1106	5	0.5512655359983114
1106	6	0.700555016416871
1106	7	0.6822174085711055
1106	8	0.5182921586036642
1106	9	0.5705728093372144
1106	10	0.6158484033083269
1106	11	0.6355349796515597
1106	12	0.4927368362929485
1106	13	0.599917633209806
1106	14	0.7740325793449898
1106	15	0.8324983501004581
1106	16	0.8550779999454079
1106	17	0.7914400528505112
1106	18	0.8209079812138214
1106	19	0.739367109176122
1106	20	0.8964329752993478
1106	21	0.9450221528216147
1106	22	0.944105340157094
1106	23	1.0251615018216995
1106	24	0.9478750235472404
1106	25	1.0466922336083115
1107	1	0.1492487643725262
1107	2	0.3087142169643684
1107	3	0.1542686137792657
1107	4	0.1474300542618181
1107	5	0.16159484766383514
1107	6	0.2407369292536643
1107	7	0.23350164869324885
1107	8	0.08183421784015366
1107	9	0.04730574032848267
1107	10	0.1922656424330853
1107	11	0.07190773093860514
1107	12	0.05703157785858082
1107	13	-0.054746665913931736
1107	14	0.11322236782618234
1107	15	0.23015160789678646
1107	16	0.20680396327887252
1107	17	0.24589190414404527
1107	18	0.11941541250570253
1107	19	0.09041050497589964
1107	20	0.15830262137739481
1107	21	0.17410331595595352
1107	22	0.20014529156754435
1107	23	0.2872060843143842
1107	24	0.10029372105974232
1107	25	0.20397304161121355
1108	1	0.1276665234354475
1108	2	0.24980084837358163
1108	3	0.1761198807918234
1108	4	0.06815258143595315
1108	5	0.19037061192346982
1108	6	0.1979701380997767
1108	7	0.2298856708837178
1108	8	0.1703722031523327
1108	9	0.10867877589506492
1108	10	0.1527589126037725
1108	11	0.17309328373706023
1108	12	0.20014509516564516
1108	13	0.17522718341475496
1108	14	0.2461145652345067
1108	15	0.3379809350809495
1108	16	0.38518496101470845
1108	17	0.29017426455543927
1108	18	0.2377584792358718
1108	19	0.15990445188919722
1108	20	0.30911095283088486
1108	21	0.20912182194220463
1108	22	0.3150979613589229
1108	23	0.3629544697401883
1108	24	0.39794016064166726
1108	25	0.4605353562380472
1109	1	-0.15951772000923015
1109	2	0.0337208670181397
1109	3	-0.061823700390487346
1109	4	0.013850301758047578
1109	5	0.043889059098006555
1109	6	0.0033732553559815415
1109	7	0.13026474521939235
1109	8	0.16478739656585617
1109	9	-0.021683794200392015
1109	10	0.21493946994292656
1109	11	0.21945830806535582
1109	12	0.06819583500269744
1109	13	0.13015681447126326
1109	14	0.20077689418732092
1109	15	0.295907013207362
1109	16	0.06963530322799016
1109	17	0.03017493043256536
1109	18	0.22182689335436834
1109	19	0.1743558880434719
1109	20	0.18518646329704325
1109	21	0.1332584409035249
1109	22	0.17434961059387888
1109	23	0.2518842625097193
1109	24	0.37310389507084096
1109	25	0.37662247199127563
1110	1	0.18291629596270553
1110	2	0.3651481731449877
1110	3	0.2142323791137457
1110	4	0.24060227639873943
1110	5	0.3065979951665554
1110	6	0.19868015137523543
1110	7	0.2886750684165733
1110	8	0.37475310439187126
1110	9	0.17019038819201882
1110	10	0.05982026312062061
1110	11	0.2062431472534172
1110	12	0.29843121858265675
1110	13	0.2656076968319212
1110	14	0.29606310418828485
1110	15	0.24051073028090286
1110	16	0.2692335283093499
1110	17	0.22837029385094354
1110	18	0.21535129557214105
1110	19	0.18210877461708463
1110	20	0.32476406063059216
1110	21	0.21403821638739373
1110	22	0.15655468187323152
1110	23	0.22691682113709533
1110	24	0.2871601901758325
1110	25	0.46103803370407803
1111	1	-0.36550563445445405
1111	2	-0.18446760121744887
1111	3	-0.28982046246706616
1111	4	-0.1453302096678044
1111	5	-0.18466614940361836
1111	6	-0.47100244024780125
1111	7	-0.2944675595178978
1111	8	-0.27856717512172224
1111	9	-0.19331925967151875
1111	10	-0.343291542317651
1111	11	-0.44361646260978205
1111	12	-0.3204702549859988
1111	13	-0.3645798770576292
1111	14	-0.3611727934641422
1111	15	-0.5062486853583437
1111	16	-0.31581333877391415
1111	17	-0.5255432131580093
1111	18	-0.31355746139458773
1111	19	-0.42958582933495393
1111	20	-0.39931284783082077
1111	21	-0.5372511561848086
1111	22	-0.48281841810987913
1111	23	-0.4104570388527691
1111	24	-0.31614689477603264
1111	25	-0.4880082633741196
1112	1	-0.47901310218843207
1112	2	-0.3294558247163444
1112	3	-0.3282733743661055
1112	4	-0.46315201449616694
1112	5	-0.5457611252936161
1112	6	-1.2404662414318182
1112	7	-0.816971219781756
1112	8	-0.607739527796106
1112	9	-0.584124786272092
1112	10	-0.6214457349111839
1112	11	-0.6207319558701272
1112	12	-0.4056139103960277
1112	13	-0.522704021669874
1112	14	-0.5038599244538418
1112	15	-0.6671998367500436
1112	16	-0.6240901104211929
1112	17	-0.6691312365359536
1112	18	-0.7229047607564283
1112	19	-0.7480332607845739
1112	20	-0.8463417181619945
1112	21	-0.8258443118892487
1112	22	-0.84955472593807
1112	23	-0.8100591343538284
1112	24	-0.8028441233197299
1112	25	-0.7506995817852484
1113	1	-0.6601910723955733
1113	2	-0.7595341994996723
1113	3	-0.7232606943362576
1113	4	-0.5174304094626478
1113	5	-0.6217977537332686
1113	6	-1.1812317354542543
1113	7	-0.6075340276655439
1113	8	-0.593890186359225
1113	9	-0.7837214042127556
1113	10	-0.7989673235078456
1113	11	-0.7581120188968171
1113	12	-0.8381367411228382
1113	13	-0.7506094998970099
1113	14	-0.7727123811671888
1113	15	-0.7350817226993129
1113	16	-0.7761710940290527
1113	17	-0.9458532492129379
1113	18	-0.8420822903058962
1113	19	-0.8463652474037531
1113	20	-0.8450881149894443
1113	21	-0.7512701424364749
1113	22	-0.8678921259501067
1113	23	-0.8303331335141877
1113	24	-0.7979830744842289
1113	25	-0.8863423016030652
1114	1	-0.9239239096699947
1114	2	-1.0081874217156894
1114	3	-1.1349024619599142
1114	4	-0.8628795611254887
1114	5	-1.1810104147219675
1114	6	-1.6184908275751013
1114	7	-1.1866445178236944
1114	8	-1.176599696368399
1114	9	-1.4459614068537405
1114	10	-1.6396135235990599
1114	11	-1.3315532105235377
1114	12	-1.2585405630435957
1114	13	-1.5193105763921793
1114	14	-1.5350771296303662
1114	15	-1.5059442008880168
1114	16	-1.4438572229583926
1114	17	-1.6464766165451792
1114	18	-1.752059762797714
1114	19	-1.4435868321166154
1114	20	-1.518291595391105
1114	21	-1.5191196822878723
1114	22	-1.5483985618803402
1114	23	-1.5117483123470592
1114	24	-1.669200480200736
1114	25	-1.5571404057921683
1115	1	-1.4110572733088276
1115	2	-2.017682319450884
1115	3	-1.5907655659571027
1115	4	-1.217422796289597
1115	5	-1.5025932817230228
1115	6	-1.8436636421005552
1115	7	-1.4802158569652484
1115	8	-1.3226814250548813
1115	9	-1.3645562939270377
1115	10	-1.3443669422012618
1115	11	-1.3990537198092596
1115	12	-1.477821458568826
1115	13	-1.5768039986505542
1115	14	-1.740252726934827
1115	15	-1.7084032205182211
1115	16	-1.6875861561250076
1115	17	-1.6027323989996916
1115	18	-1.7325254903655036
1115	19	-1.7815074922531196
1115	20	-1.720455612571861
1115	21	-1.7780928089584336
1115	22	-1.9906035170388066
1115	23	-1.960042164519244
1115	24	-1.965119760097437
1115	25	-2.033117798848558
1116	1	-0.9375171796095287
1116	2	-0.9155804733661235
1116	3	-1.049195074002899
1116	4	-0.9098335193825164
1116	5	-0.7606108942603527
1116	6	-1.0192021030609055
1116	7	-1.1282553959278658
1116	8	-1.0785557154287702
1116	9	-1.0771519636928986
1116	10	-1.2411643125333285
1116	11	-1.0883290768182476
1116	12	-1.1370442729485788
1116	13	-1.0573659032245715
1116	14	-1.4767894414845273
1116	15	-1.3656363104438114
1116	16	-1.3685162724553095
1116	17	-1.474325183759042
1116	18	-1.51497030557384
1116	19	-1.3348413610647043
1116	20	-1.3465137692678226
1116	21	-1.3450322877032406
1116	22	-1.3650545344005138
1116	23	-1.529093024619442
1116	24	-1.7325474634875775
1116	25	-1.807895880690296
1201	1	0.7306421486332937
1201	2	1.002116982171195
1201	3	0.9547734631491096
1201	4	0.8270952848231019
1201	5	0.8303621704335455
1201	6	0.8921877175025443
1201	7	0.8033160813511699
1201	8	0.96718554070641
1201	9	0.984873774312284
1201	10	1.1503530879757236
1201	11	1.0313395642055596
1201	12	1.157188463320125
1201	13	1.130065212326862
1201	14	1.2522240935662623
1201	15	1.21251929110079
1201	16	1.1535188219411623
1201	17	1.2334263485482637
1201	18	1.2390458064491199
1201	19	1.36885243120755
1201	20	1.3444596918833298
1201	21	1.354885249187113
1201	22	1.3396204895851511
1201	23	1.4028194059014254
1201	24	1.4927591962014404
1201	25	1.469420024381975
1202	1	0.47997034554424545
1202	2	0.5403707830591848
1202	3	0.5243550126180807
1202	4	0.45191920461078183
1202	5	0.44375598218846335
1202	6	0.384652822025231
1202	7	0.4305619977201047
1202	8	0.36891225683697115
1202	9	0.48511731158040305
1202	10	0.61662179260653
1202	11	0.5636013335591628
1202	12	0.6362138161032291
1202	13	0.5113098907146778
1202	14	0.5714170442693387
1202	15	0.6733739039641691
1202	16	0.5404584901954337
1202	17	0.6397858077110286
1202	18	0.5551534905111737
1202	19	0.6731181156072381
1202	20	0.6140960372376227
1202	21	0.6831587267793751
1202	22	0.7782523267325203
1202	23	0.7658264638697503
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2211	4	-0.3305305786867905
2211	5	-0.32138353890523064
2211	6	-0.2544134299650267
2211	7	-0.3578921801332058
2211	8	-0.3423076905044269
2211	9	-0.2664184490736261
2211	10	-0.2932894691772603
2211	11	-0.2919035303600097
2211	12	-0.28127779499760663
2211	13	-0.2238470737571845
2211	14	-0.41454942087934654
2211	15	-0.3731624012551862
2211	16	-0.2516631190210319
2211	17	-0.4228820783209599
2211	18	-0.31270695554832884
2211	19	-0.3423828066962571
2211	20	-0.3312555358646563
2211	21	-0.34651576145887475
2211	22	-0.37046587022033606
2211	23	-0.48220039443781104
2211	24	-0.4375090516712987
2211	25	-0.4276562959638852
2212	1	-0.3744309640547385
2212	2	-0.5599154057648867
2212	3	-0.518901989117488
2212	4	-0.4495564155126601
2212	5	-0.5458245511851487
2212	6	-0.6013879455004272
2212	7	-0.6505377317007586
2212	8	-0.7531938533802105
2212	9	-0.8235232225400537
2212	10	-0.9080073679724165
2212	11	-0.8455639081067261
2212	12	-0.8102592523072403
2212	13	-0.7427545994994915
2212	14	-0.8718863155477621
2212	15	-0.8771750759730068
2212	16	-0.9081810952327842
2212	17	-0.8444095515691501
2212	18	-0.8618805140499504
2212	19	-0.8529532590175322
2212	20	-0.9670142892773512
2212	21	-0.8589082044670135
2212	22	-0.938262622066695
2212	23	-1.0994282885905378
2212	24	-1.1172687258528669
2212	25	-0.9716702052321438
2213	1	-0.745741002094487
2213	2	-0.9771904302465231
2213	3	-0.7804498410296254
2213	4	-0.7690295775625913
2213	5	-0.8586423618673962
2213	6	-0.6840299575232223
2213	7	-0.7673184814043381
2213	8	-0.6853689735167805
2213	9	-0.9517484901590549
2213	10	-0.8059901698373153
2213	11	-0.8655218140093339
2213	12	-0.7894964029654901
2213	13	-0.7773733402617822
2213	14	-0.9554303086696905
2213	15	-0.8478820484128349
2213	16	-0.8812628977332437
2213	17	-1.0393431486737228
2213	18	-1.1252424516821407
2213	19	-0.9815464302329531
2213	20	-1.1278116178527924
2213	21	-1.1285536392278317
2213	22	-1.0526171833552382
2213	23	-1.0511825467660216
2213	24	-1.219720000848703
2213	25	-1.338922686359183
2214	1	-0.7174686971723858
2214	2	-0.6318449720991914
2214	3	-0.7844329610988758
2214	4	-0.6102746806074038
2214	5	-0.8500239121356188
2214	6	-0.7233730776690308
2214	7	-0.7343110774138495
2214	8	-0.8016410047309677
2214	9	-0.8681759415316321
2214	10	-1.0097477679833773
2214	11	-0.8708564865336044
2214	12	-0.9539156660691361
2214	13	-0.7488012486117341
2214	14	-0.9576953373635675
2214	15	-1.0878310583510142
2214	16	-1.2202343588980682
2214	17	-1.0318185735789456
2214	18	-0.9536842928176625
2214	19	-0.931980654440892
2214	20	-1.0085866993311399
2214	21	-1.0384436823368262
2214	22	-0.9947009236217355
2214	23	-1.2945103182141722
2214	24	-1.3097471754509087
2214	25	-1.3800547174106796
2215	1	-1.4906146011956558
2215	2	-3.20722063860428
2215	3	-1.759226950434794
2215	4	-1.3533672642234933
2215	5	-1.3449342369053738
2215	6	-1.3205616380180416
2215	7	-1.3242286731482196
2215	8	-1.257772851555984
2215	9	-1.5545550011535454
2215	10	-1.5526464592097398
2215	11	-1.5262127637997196
2215	12	-1.5961842070289052
2215	13	-1.6925724555918649
2215	14	-1.8486308681439851
2215	15	-1.738824756848345
2215	16	-1.9376362008757653
2215	17	-1.9906164407626576
2215	18	-1.8271695085229496
2215	19	-1.8340649976304206
2215	20	-1.8176026954532887
2215	21	-1.899810623684381
2215	22	-1.9336248208587747
2215	23	-2.271243231447329
2215	24	-2.241884839047607
2215	25	-2.2642040145507636
2216	1	-1.2297116861166764
2216	2	-1.9493886899463924
2216	3	-1.337242119526696
2216	4	-1.3180377177978215
2216	5	-1.2742716701101173
2216	6	-1.449942657639106
2216	7	-1.5500651351540427
2216	8	-1.7657428894844998
2216	9	-1.6838996601616358
2216	10	-1.6804310858036189
2216	11	-1.8150364609144276
2216	12	-1.923898011486159
2216	13	-1.9300797479791285
2216	14	-1.8941200793479638
2216	15	-1.9280203743986206
2216	16	-1.8619704600537546
2216	17	-1.8720667150768477
2216	18	-1.9976363996372726
2216	19	-1.7803089578894244
2216	20	-2.056552369354179
2216	21	-1.9696186002764833
2216	22	-1.9591105998061593
2216	23	-2.2247470397004463
2216	24	-2.1846048305989783
2216	25	-2.144468979201072
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31258.0
3	6.0
4	445.0
5	3648.0
6	5524.0
7	4889.0
8	3535.0
9	2601.0
10	2935.0
11	3187.0
12	2633.0
13	2056.0
14	1787.0
15	1772.0
16	1779.0
17	1908.0
18	2602.0
19	4269.0
20	5775.0
21	6618.0
22	7415.0
23	7801.0
24	8264.0
25	8929.0
26	9782.0
27	10625.0
28	11805.0
29	14039.0
30	17381.0
31	22260.0
32	28957.0
33	37868.0
34	51243.0
35	69762.0
36	103310.0
37	176136.0
38	471430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08173732963731	18.70241201447016	14.52722958382969	22.68862107206284
2	15.928907037313337	23.84319063262092	36.606070124465035	23.62183220560071
3	18.19715695050051	28.081002657398052	29.424183892643214	24.297656499458224
4	12.485495980750876	18.976142742232387	34.95438104261434	33.5839802344024
5	14.222955427244678	37.657846384819166	31.639629731463657	16.4795684564725
6	30.072850051038657	37.90183128451653	15.926766068452874	16.09855259599194
7	25.677029168091686	33.859397793024506	21.53423883076365	18.929334208120164
8	26.073820877761435	32.66392377123694	19.994870157402413	21.26738519359921
9	26.271162779334727	15.692390648067036	20.235648049586604	37.80079852301163
10	16.104282300053313	29.054581721628246	30.78025868139328	24.06087729692516
11	32.29308171162092	25.032521495596182	23.068493628753277	19.605903164029613
12	23.818730864770586	27.73752114745502	29.050037561544656	19.393710426229738
13	29.001855961020457	23.59243200699454	25.769935874670715	21.635776157314293
14	22.438406862381573	23.218905051601347	28.8040998402535	25.538588245763577
15	22.906277350352397	28.692706601780614	26.90467526869335	21.496340779173636
16	21.184399149476015	28.404429914223417	27.333335078355876	23.077835857944695
17	21.625620878080614	26.11487585928305	28.2883962870771	23.971106975559238
18	23.036852018743133	24.052526842273778	31.014200428708648	21.896420710274437
19	24.35758625352801	24.280111849308796	31.463681691873475	19.89862020528972
20	23.349619173086484	24.44865161971401	31.466249051204425	20.735480155995077
21	22.812640953541713	24.766033479001685	31.58720340321285	20.834122164243755
22	21.83698965481743	25.029269765161388	31.853269053264867	21.280471526756315
23	21.322674279113553	24.97729439980876	32.112218448376495	21.587812872701196
24	21.226791648526905	25.703001152510964	32.09503111605114	20.97517608291099
25	21.18144556912108	25.428064414250144	32.2966377771535	21.09385223947528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8680.0
1	8680.0
2	8016.5
3	7353.0
4	7353.0
5	7353.0
6	11729.0
7	16105.0
8	16105.0
9	16105.0
10	15088.0
11	14071.0
12	14071.0
13	14071.0
14	11305.0
15	8539.0
16	8539.0
17	8539.0
18	14733.5
19	20928.0
20	20928.0
21	20928.0
22	30315.0
23	39702.0
24	39702.0
25	39702.0
26	52846.0
27	65990.0
28	65990.0
29	65990.0
30	73191.0
31	80392.0
32	80392.0
33	80392.0
34	83671.0
35	86950.0
36	86950.0
37	86950.0
38	89232.0
39	91514.0
40	91514.0
41	91514.0
42	99367.0
43	107220.0
44	107220.0
45	107220.0
46	113340.5
47	119461.0
48	119461.0
49	119461.0
50	121821.0
51	124181.0
52	124181.0
53	124181.0
54	117458.5
55	110736.0
56	110736.0
57	110736.0
58	96946.5
59	83157.0
60	83157.0
61	83157.0
62	71688.0
63	60219.0
64	60219.0
65	60219.0
66	50033.0
67	39847.0
68	39847.0
69	39847.0
70	32273.5
71	24700.0
72	24700.0
73	24700.0
74	20173.5
75	15647.0
76	15647.0
77	15647.0
78	13079.5
79	10512.0
80	10512.0
81	10512.0
82	8331.0
83	6150.0
84	6150.0
85	6150.0
86	4494.5
87	2839.0
88	2839.0
89	2839.0
90	2020.5
91	1202.0
92	1202.0
93	1202.0
94	662.5
95	123.0
96	123.0
97	123.0
98	69.5
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3267221178223644
2	0.01125424651510948
3	0.0
4	0.0
5	0.050513245986421616
6	0.0038386577260838537
7	0.004362111052368015
8	0.0
9	0.010294582083588516
10	0.01666326422004582
11	0.0060197132522678614
12	0.010032855420446437
13	0.017186717546329982
14	0.0037514155050364937
15	0.01962949973565607
16	0.010207339862541156
17	0.0065431665785520236
18	8.724222104736031E-5
19	0.004274868831320655
20	0.0038386577260838537
21	0.002006571084089287
22	0.0
23	0.0035769310629417727
24	0.0035769310629417727
25	0.0024427821893260888
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1146234.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.69214841413162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.50284620065024	56.73817865912494
2	5.68232055242632	7.124737668187625
3	1.294578251582327	2.4347967564571875
4	0.5882995884543197	1.475270604454031
5	0.3658006567615724	1.1466414531841658
6	0.24484595964760544	0.9209951544496898
7	0.17320016754928005	0.760080342654635
8	0.13892384042611955	0.6967547217804338
9	0.11791564470249687	0.6653146588233252
>10	0.7633925505225028	9.375811423230031
>50	0.07738229014837215	3.3908856906852893
>100	0.047556956720453375	5.233843184413923
>500	6.993667639225529E-4	0.26087658951047465
>1k	0.0012588601750605952	1.785886595620278
>5k	2.7974670556902117E-4	1.2452801504666389
>10k+	6.993667639225529E-4	6.7446463469573645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	23261	2.029341303782648	No Hit
TATCAACGCAGAGTACTTTTTTTTT	17915	1.56294439006346	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	13052	1.1386854691101467	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	11455	0.9993596420975124	No Hit
GGTATCAACGCAGAGTACTTTTTTT	11232	0.979904626803951	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	8530	0.7441761455339835	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	5671	0.4947506355595804	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4477	0.3905834236290321	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	3319	0.2895569316561889	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT	3057	0.2666994697417805	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	1994	0.17396098876843646	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	1804	0.157384966769438	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	1633	0.1424665469703394	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	1450	0.12650122051867244	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1372	0.11969632727697835	No Hit
TCAACGCAGAGTACTTTTTTTTTTT	1260	0.109925198519674	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	8.724222104736031E-5	0.0	0.0	0.0	0.0
4	8.724222104736031E-5	0.0	0.0	0.0	0.0
5	8.724222104736031E-5	0.0	0.0	0.0	0.0
6	8.724222104736031E-5	0.0	0.0	0.0	0.0
7	8.724222104736031E-5	0.0	0.0	0.0	0.0
8	8.724222104736031E-5	0.0	0.0	8.724222104736031E-5	0.0
9	8.724222104736031E-5	0.0	0.0	8.724222104736031E-5	0.0
10	8.724222104736031E-5	0.0	0.0	8.724222104736031E-5	0.0
11	8.724222104736031E-5	0.0	0.0	1.7448444209472062E-4	0.0
12	8.724222104736031E-5	0.0	0.0	1.7448444209472062E-4	1.7448444209472062E-4
13	8.724222104736031E-5	0.0	0.0	2.6172666314208096E-4	2.6172666314208096E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2600	0.0	14.85003	1
AACCGCG	60	2.5743377E-5	14.245746	7
CGTCAAT	80	2.0077514E-6	13.056892	19
CCGTCAA	85	3.961659E-6	12.288303	18
TCCGTCA	85	3.961659E-6	12.288303	17
GCGTTAT	55	0.002988114	12.133357	1
GATCTAT	55	0.002988114	12.133357	1
TATTAGG	55	0.0030614296	12.094159	2
CTATCCT	90	7.436998E-6	11.613726	4
CTCTTAC	75	2.0065061E-4	11.440022	1
GTATCAA	6325	0.0	11.319443	1
ACAGGAC	220	0.0	11.229311	3
CCTAATA	60	0.005862321	11.086312	2
CCTACAC	60	0.005866132	11.085344	3
AAGGCGT	60	0.005889045	11.079541	6
GCGCGAC	60	0.0058928714	11.078574	19
TACGCTA	130	3.2714524E-8	10.959222	9
GTATTAA	105	3.3205743E-6	10.89526	1
ACGCTAT	125	2.2436325E-7	10.637752	10
CGCGGTC	90	9.529544E-5	10.553325	10
>>END_MODULE