FastQCFastQC Report
Thu 2 Feb 2017
SRR4050268_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050268_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129740
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT154241.3652698851063076No Hit
TATCAACGCAGAGTACTTTTTTTTT95640.8465664666206383No Hit
GGTATCAACGCAGAGTACTTTTTTT74230.6570538353957548No Hit
GTACTTTTTTTTTTTTTTTTTTTTT69040.6111140616425018No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51960.4599288331828562No Hit
GAGTACTTTTTTTTTTTTTTTTTTT48340.4278860622798166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA41750.3695540566856091No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN37390.3309611060952078No Hit
GCAGAGTACTTTTTTTTTTTTTTTT25790.22828261369872713No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN17170.15198187193513552No Hit
ATCAACGCAGAGTACTTTTTTTTTT15210.13463274735779912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCTC551.9600488E-413.81763216
CTAGACC500.001498536713.3006544
AAATCGC500.001499544113.29946914
TGTGCCG604.0933644E-412.66559610
TCTAGAC853.9383776E-612.29472353
CGACGAC658.0230494E-411.69184114
CAAATCG658.0230494E-411.69184113
GGTTAGA907.3995434E-611.61893751
GTATCAA72950.011.2200311
GAGTATA600.005844540411.0908041
GGTATCA30350.010.8689361
GTAGGTT700.001490228710.8576774
CTTATAC700.001490228710.8576773
AACACCG700.001490783210.8571935
TATGGCG700.001491337910.856709516
CGAACCT1053.4644563E-610.85670916
GTAGGAC1158.8007255E-710.739663
GTATTAG1252.2162567E-710.6471721
ATCGCCA1002.397794E-510.44958316
TTGAACA1659.258656E-1010.3641474
Produced by FastQC (version 0.11.5)