##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050268_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1129740
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.237759130419388	31.0	30.0	34.0	23.0	34.0
2	29.122747711862907	31.0	30.0	34.0	22.0	34.0
3	29.265629259829694	31.0	30.0	34.0	23.0	34.0
4	32.519652309380916	35.0	33.0	37.0	23.0	37.0
5	32.66670826915927	37.0	33.0	37.0	24.0	37.0
6	32.71313930638908	37.0	35.0	37.0	25.0	37.0
7	32.51886274718077	37.0	35.0	37.0	22.0	37.0
8	32.32326553012197	37.0	35.0	37.0	18.0	37.0
9	33.55459132189707	39.0	35.0	39.0	16.0	39.0
10	33.51034131747128	39.0	34.0	39.0	17.0	39.0
11	33.5473666507338	39.0	35.0	39.0	17.0	39.0
12	33.44569901039177	39.0	34.0	39.0	16.0	39.0
13	33.36329243896826	39.0	34.0	39.0	15.0	39.0
14	34.319149538831944	40.0	34.0	41.0	13.0	41.0
15	34.29552463398658	39.0	34.0	41.0	13.0	41.0
16	34.358583390868695	39.0	34.0	41.0	15.0	41.0
17	34.399908828580024	39.0	34.0	41.0	16.0	41.0
18	34.338518597199354	40.0	34.0	41.0	13.0	41.0
19	34.38386177350541	40.0	34.0	41.0	12.0	41.0
20	34.38045833554623	40.0	34.0	41.0	12.0	41.0
21	34.31340042841716	40.0	34.0	41.0	10.0	41.0
22	34.24360560837007	39.0	34.0	41.0	10.0	41.0
23	34.16329863508418	39.0	34.0	41.0	2.0	41.0
24	34.11231433781224	39.0	34.0	41.0	2.0	41.0
25	33.80997397631313	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1031512734274038
1101	2	1.0042920987357036
1101	3	1.0654219101344964
1101	4	1.178721278626476
1101	5	1.3380751478333934
1101	6	1.1056586957437489
1101	7	1.1721543891084067
1101	8	0.9162355182164177
1101	9	1.1157741470230036
1101	10	1.0383394612704109
1101	11	0.9512500109222515
1101	12	0.9587630182644915
1101	13	0.9555865447161267
1101	14	1.041485792058218
1101	15	1.1148307796994459
1101	16	1.1442094436319508
1101	17	1.093302265087523
1101	18	1.148660222616975
1101	19	1.2183968961090699
1101	20	1.1400419112429816
1101	21	1.162730497383123
1101	22	1.2236747518663122
1101	23	1.2888883646195382
1101	24	1.3833548125627289
1101	25	1.3296425482637204
1102	1	1.2542447597189899
1102	2	1.1806838890318225
1102	3	1.2252461733737654
1102	4	1.3582465887328112
1102	5	1.3556384968638326
1102	6	1.2213496044054821
1102	7	1.2913653440352917
1102	8	1.2467643218137638
1102	9	1.484930516152815
1102	10	1.6426101360098713
1102	11	1.5072963468875074
1102	12	1.6059646847541202
1102	13	1.5078930159361335
1102	14	1.7224141046784567
1102	15	1.8044574128474835
1102	16	1.7767408083358873
1102	17	1.8108458452076661
1102	18	1.8267899066684805
1102	19	1.781685439582965
1102	20	1.7424094595440351
1102	21	1.7443451157546335
1102	22	1.7785747860930883
1102	23	2.0099755956738434
1102	24	1.8441430314724272
1102	25	1.9121806993099923
1103	1	1.1298241329408505
1103	2	1.150913018862358
1103	3	1.2070645867097198
1103	4	1.3170810753553894
1103	5	1.197600580206263
1103	6	1.142010035306356
1103	7	1.0948468586642406
1103	8	1.1933064550714292
1103	9	1.2697402052376745
1103	10	1.2924158595260593
1103	11	1.337906535124688
1103	12	1.1691391042894637
1103	13	1.2203283480615994
1103	14	1.3953902093985704
1103	15	1.4551353795658102
1103	16	1.5398235468339223
1103	17	1.6408448265294737
1103	18	1.5090805501092888
1103	19	1.4578194466991619
1103	20	1.5567917403645097
1103	21	1.5005526859888576
1103	22	1.4781729539275972
1103	23	1.5100592728110627
1103	24	1.6733425323201132
1103	25	1.5141294670731966
1104	1	1.0926678365067346
1104	2	1.2095633009931603
1104	3	1.030402947324415
1104	4	1.2874511249908593
1104	5	1.0912572886259468
1104	6	1.1210690791397369
1104	7	1.087003699553783
1104	8	0.9965501233120122
1104	9	1.233223965461221
1104	10	1.316089810463339
1104	11	1.2848967254954573
1104	12	1.2423735039266077
1104	13	1.1132905542807379
1104	14	1.2376494679274472
1104	15	1.1800687802344214
1104	16	1.3186524617899806
1104	17	1.3110085832890803
1104	18	1.2597761240724807
1104	19	1.3626194857970617
1104	20	1.2831115732038256
1104	21	1.3121818252434068
1104	22	1.4704425442484634
1104	23	1.3099414646600067
1104	24	1.3897717106725622
1104	25	1.2764215129652143
1105	1	0.9498976840619946
1105	2	1.1027641199000975
1105	3	0.9694071529069888
1105	4	1.0173741358609405
1105	5	1.0803221230974884
1105	6	1.025103371376268
1105	7	1.0695459963900618
1105	8	1.0557719990124141
1105	9	1.2741933117715618
1105	10	1.1394179195294427
1105	11	1.1344301006717359
1105	12	0.994927503501124
1105	13	1.1182807938634909
1105	14	1.4365545276838176
1105	15	1.3993285505158113
1105	16	1.3674061425391173
1105	17	1.3492033596455641
1105	18	1.3970972017331889
1105	19	1.2909423495795522
1105	20	1.3710802064484753
1105	21	1.3327187681887978
1105	22	1.3569532367764054
1105	23	1.3850122729380772
1105	24	1.2015500265602626
1105	25	1.2046154310395423
1106	1	0.783692239986145
1106	2	0.8610117578842811
1106	3	0.8571526700343632
1106	4	0.8209059694249845
1106	5	0.8645182782845993
1106	6	0.900994413148652
1106	7	0.777476455146406
1106	8	0.8590138664403639
1106	9	0.9544204979378819
1106	10	0.9682021214379262
1106	11	1.1631858429409405
1106	12	1.253050294954491
1106	13	1.0970703079822783
1106	14	1.2945907895600612
1106	15	1.193270406168672
1106	16	1.1736746274008567
1106	17	1.216279967854902
1106	18	1.2288846243369704
1106	19	1.12040735822292
1106	20	1.1492097024098484
1106	21	1.3452103252264038
1106	22	1.258310669517492
1106	23	1.360916700713311
1106	24	1.3706305845993256
1106	25	1.4451952712589957
1107	1	0.6802878673113817
1107	2	0.6522234599180088
1107	3	0.7207750115093674
1107	4	0.8235221182576851
1107	5	0.8952743779991152
1107	6	0.6885173453282647
1107	7	0.5812587523212898
1107	8	0.5673917560584982
1107	9	0.5194563228259597
1107	10	0.7273954319453608
1107	11	0.48429304072941903
1107	12	0.4475315289918953
1107	13	0.5913305864495157
1107	14	0.7187241085045741
1107	15	0.6442301512685731
1107	16	0.5863742858844248
1107	17	0.5578977724918133
1107	18	0.6499766702634631
1107	19	0.7294086543066527
1107	20	0.8454497515062442
1107	21	0.6512637666066823
1107	22	0.48366572996688006
1107	23	0.5119916653680434
1107	24	0.44154347883387857
1107	25	0.526472475119121
1108	1	0.674188699241629
1108	2	0.8657212154944425
1108	3	0.7465732211384264
1108	4	0.7451393848670946
1108	5	0.8722761423237841
1108	6	0.6776585316118044
1108	7	0.6479144630175213
1108	8	0.7994991576433961
1108	9	1.0699390573534941
1108	10	1.0086713683965982
1108	11	0.9757292961027559
1108	12	0.8707348273591435
1108	13	0.9382668940187457
1108	14	1.0818535470937718
1108	15	1.18467887060865
1108	16	1.0312331967576043
1108	17	1.0970586485146683
1108	18	1.0066152131607353
1108	19	1.1557581766366738
1108	20	1.1444970988694223
1108	21	1.2234816994631217
1108	22	1.2537805299325697
1108	23	1.157073003112707
1108	24	1.2295010068775412
1108	25	1.4093882929564714
1109	1	0.46891157390820126
1109	2	0.5703769806740553
1109	3	0.3891360355016573
1109	4	0.6586608812085473
1109	5	0.5950099586278341
1109	6	0.3509043629295192
1109	7	0.24264679246079623
1109	8	0.3405387168176688
1109	9	0.35634464266568955
1109	10	0.4839542507824106
1109	11	0.4216149464068337
1109	12	0.505982817733404
1109	13	0.34964973282193057
1109	14	0.47021432842097965
1109	15	0.4135680055807711
1109	16	0.5035951359711603
1109	17	0.4215077296852243
1109	18	0.46638157656220613
1109	19	0.5440104870667852
1109	20	0.4228233832134123
1109	21	0.547178931609622
1109	22	0.5236473794779286
1109	23	0.578939311274965
1109	24	0.5682520567786895
1109	25	0.7541955806465452
1110	1	-0.07888651081673714
1110	2	0.09449092784544888
1110	3	0.028792814738658024
1110	4	-0.08590501107742199
1110	5	-0.01887391682021189
1110	6	-0.15504726449968587
1110	7	-0.1936666345987632
1110	8	-0.17454320019350433
1110	9	-0.329290312128947
1110	10	-0.22280286277774763
1110	11	-0.27005241350115483
1110	12	-0.4477532707217762
1110	13	-0.22156905775529623
1110	14	-0.2535030847158808
1110	15	-0.1968406209936404
1110	16	0.029270985975443864
1110	17	-0.24603483231767598
1110	18	-0.1357274443760943
1110	19	-0.2028238113790266
1110	20	-0.06210796178325495
1110	21	-0.2017941670102914
1110	22	-0.10868515451802097
1110	23	-0.12678577694752846
1110	24	-0.13444929365540048
1110	25	-0.28682260397747683
1111	1	-0.5740669675493635
1111	2	-0.48603230037710077
1111	3	-0.5020724106719854
1111	4	-0.601467367907901
1111	5	-0.6604312792650688
1111	6	-0.7674409228843757
1111	7	-0.8330417979931042
1111	8	-0.565838437876419
1111	9	-0.6676738141157657
1111	10	-0.5574794294068397
1111	11	-0.6026108899415661
1111	12	-0.7329286375993078
1111	13	-0.6184886685728728
1111	14	-0.6527075797739528
1111	15	-0.762079574981243
1111	16	-0.7837653706874121
1111	17	-0.6572848552137032
1111	18	-0.6755015813625818
1111	19	-0.6500463990125098
1111	20	-0.5386363475776221
1111	21	-0.5619633033945348
1111	22	-0.5084466473497642
1111	23	-0.661810307606828
1111	24	-0.529147018984176
1111	25	-0.6493318525827618
1112	1	-0.2437139427341286
1112	2	-0.15584505516905622
1112	3	-0.4142331243869748
1112	4	-0.3033504215682683
1112	5	-0.13748600446458425
1112	6	-0.20075789479253103
1112	7	-0.2478288712372887
1112	8	-0.34632960803359225
1112	9	-0.590058036678272
1112	10	-0.4993560077885064
1112	11	-0.3272961750266248
1112	12	-0.32881044349529276
1112	13	-0.44138745752425734
1112	14	-0.5691517994833788
1112	15	-0.5376295774107689
1112	16	-0.5403889545774803
1112	17	-0.4293589425878679
1112	18	-0.30508627746231554
1112	19	-0.3614908824622276
1112	20	-0.3420445996083572
1112	21	-0.45125032895759887
1112	22	-0.3776519939759524
1112	23	-0.5240053595272869
1112	24	-0.42742460004663485
1112	25	-0.6736432500866627
1113	1	-0.874411737161271
1113	2	-0.7150678922624145
1113	3	-0.8657105457234486
1113	4	-0.7842095016723185
1113	5	-0.9126242173461883
1113	6	-0.9217855920706768
1113	7	-0.9096273172696989
1113	8	-0.9270858438604357
1113	9	-0.8323118584844025
1113	10	-1.1047050720120808
1113	11	-0.8728152360721708
1113	12	-0.8368618990359025
1113	13	-0.8817606498527368
1113	14	-1.07846744815145
1113	15	-1.1772897580381425
1113	16	-0.9516147862328452
1113	17	-1.0896922993994167
1113	18	-1.0750491165259035
1113	19	-0.9951832388983632
1113	20	-1.134604134439499
1113	21	-1.0988940719277451
1113	22	-1.0310827546532764
1113	23	-1.0659182146746673
1113	24	-1.1113789806585643
1113	25	-1.1244129362700264
1114	1	-1.2792093005173797
1114	2	-1.1787804647615445
1114	3	-1.3711844404272249
1114	4	-1.2411307146094153
1114	5	-1.2595921550614406
1114	6	-1.2812873253187504
1114	7	-1.101235367618031
1114	8	-1.3846135533927395
1114	9	-1.4438167833769882
1114	10	-1.2257737120013132
1114	11	-1.2854425652995545
1114	12	-1.5351666534082895
1114	13	-1.4047864859065413
1114	14	-1.3224137006335255
1114	15	-1.4096263334989416
1114	16	-1.4884294801378672
1114	17	-1.808657903680377
1114	18	-1.572252220666627
1114	19	-1.4473541461331365
1114	20	-1.3639334684010649
1114	21	-1.4281831597568697
1114	22	-1.359482781577519
1114	23	-1.5589578774485346
1114	24	-1.5840165186610093
1114	25	-1.4796972394369874
1115	1	-1.4577016276808017
1115	2	-1.4808489665808757
1115	3	-1.60219021483001
1115	4	-1.7632791088508313
1115	5	-1.4729879785070992
1115	6	-1.6214431550925212
1115	7	-1.613298332338804
1115	8	-1.555759337287693
1115	9	-2.2009260224214415
1115	10	-1.8756214417909014
1115	11	-1.9894352088686098
1115	12	-1.8479237329843023
1115	13	-1.8664223030240876
1115	14	-2.0443920268477314
1115	15	-2.063043514324761
1115	16	-2.070240111367106
1115	17	-1.832131246505874
1115	18	-1.7647751600749544
1115	19	-1.8660082356759276
1115	20	-1.976484884316271
1115	21	-1.7968748224684603
1115	22	-1.9826832245454042
1115	23	-1.9396432911489043
1115	24	-2.0789045901686833
1115	25	-1.9725682250405328
1116	1	-1.5579391975438739
1116	2	-1.6122851795596453
1116	3	-1.440847028648907
1116	4	-1.779749394465945
1116	5	-1.4685907666546392
1116	6	-1.4917575034976238
1116	7	-1.5413639398730332
1116	8	-1.6811830315831848
1116	9	-2.051975119602833
1116	10	-2.7839710971685
1116	11	-2.092443172727531
1116	12	-2.009831844892723
1116	13	-1.9471473559748063
1116	14	-1.8553196649977508
1116	15	-2.6177680377682897
1116	16	-2.26064398898896
1116	17	-2.1974298867880933
1116	18	-2.1966737178319633
1116	19	-2.031204516374949
1116	20	-2.072993545920447
1116	21	-1.9512292664327902
1116	22	-2.0278668785345957
1116	23	-1.9957998304741054
1116	24	-1.8989932339219138
1116	25	-2.124268713462211
1201	1	1.0941568613077735
1201	2	1.0233554562293783
1201	3	0.984100188114251
1201	4	1.1136128645238017
1201	5	0.989334022212411
1201	6	0.9685478425479488
1201	7	0.831299322994127
1201	8	0.7790237193596141
1201	9	1.0886341118201202
1201	10	1.2857552267280354
1201	11	1.1283112623922449
1201	12	1.2067065706806517
1201	13	0.8651023386127505
1201	14	1.232850582519113
1201	15	1.3546581984881527
1201	16	1.2520757751795202
1201	17	1.2853757248335143
1201	18	1.2744349293237107
1201	19	1.2455804865463875
1201	20	1.2724913295243923
1201	21	1.2857648112216395
1201	22	1.4587397900796262
1201	23	1.4049856106476852
1201	24	1.441333490994431
1201	25	1.3688299613133807
1202	1	0.937650111346457
1202	2	0.9853583960406134
1202	3	0.9596842821882134
1202	4	0.9111169261051089
1202	5	0.8528320107626257
1202	6	0.9460466820961457
1202	7	0.9106558524696027
1202	8	1.0106668224077353
1202	9	0.9696321970746524
1202	10	1.1504059307354595
1202	11	1.2277020251958959
1202	12	1.1090236061131193
1202	13	1.049344370177046
1202	14	1.1466575413617548
1202	15	0.9097303399085419
1202	16	1.0754608130876093
1202	17	1.0426333838154136
1202	18	1.2380886118241037
1202	19	1.0443146270384176
1202	20	1.0295879758186857
1202	21	1.2058116161110135
1202	22	1.0419538547554765
1202	23	1.1113951727705427
1202	24	1.0513738166945714
1202	25	1.2456513871218249
1203	1	0.6710723068106006
1203	2	0.6982116749899596
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2211	6	-0.15644692404195837
2211	7	-0.3122559703623011
2211	8	-0.16549642451158775
2211	9	-0.2049601942296988
2211	10	-0.25157326015037995
2211	11	-0.23511766789873434
2211	12	-0.23253068358695117
2211	13	-0.10663534384295303
2211	14	-0.36732830495305535
2211	15	-0.2701101041742291
2211	16	-0.18043607135786743
2211	17	-0.1883753932131853
2211	18	-0.36323230191688793
2211	19	-0.4141964750308702
2211	20	-0.3182141341557383
2211	21	-0.4248295138587608
2211	22	-0.4215749757490599
2211	23	-0.48657622922899435
2211	24	-0.44544596358908706
2211	25	-0.5112983048102819
2212	1	-1.0017111912339622
2212	2	-0.8674385286677655
2212	3	-0.8891580649309105
2212	4	-1.084343042081528
2212	5	-1.1820964091737487
2212	6	-1.131034441975963
2212	7	-1.147392486165959
2212	8	-1.14690321117304
2212	9	-1.2877853974817342
2212	10	-1.0723538783843694
2212	11	-1.1651929907107856
2212	12	-1.1193035412887937
2212	13	-1.1323342265002267
2212	14	-1.3448423260825706
2212	15	-1.1927020566980318
2212	16	-1.3131482360463593
2212	17	-1.3700234737314219
2212	18	-1.1588824910391011
2212	19	-1.1999302474755424
2212	20	-1.3664386678716696
2212	21	-1.2152293877772848
2212	22	-1.200225360514935
2212	23	-1.4370539258508828
2212	24	-1.3206160648890304
2212	25	-1.4817533937194582
2213	1	-1.0752683452186744
2213	2	-1.028168897198995
2213	3	-1.000916255866116
2213	4	-1.186066424584812
2213	5	-1.2238786982541257
2213	6	-1.1956156454227305
2213	7	-1.1451591479643142
2213	8	-1.17970650702037
2213	9	-1.1700300545091409
2213	10	-1.4194706205098768
2213	11	-1.1596157305968688
2213	12	-1.0372816095425321
2213	13	-1.25452886162023
2213	14	-1.059108914856246
2213	15	-1.1035176206919957
2213	16	-1.20688210976234
2213	17	-1.2608059332027253
2213	18	-1.292651538310416
2213	19	-1.3643544478552698
2213	20	-1.2707854620881136
2213	21	-1.3036531500089197
2213	22	-1.355734464258667
2213	23	-1.2469858911885012
2213	24	-1.2514403918448735
2213	25	-1.219232703392798
2214	1	-1.1670669890515697
2214	2	-1.2472008183491923
2214	3	-1.2500727702510517
2214	4	-1.373966512353249
2214	5	-1.2404823094409565
2214	6	-1.270618715820202
2214	7	-1.538133080040133
2214	8	-1.309607785004669
2214	9	-1.5538846564608164
2214	10	-1.8835689371332585
2214	11	-1.8396346322632695
2214	12	-1.3920992379175985
2214	13	-1.5020638067374996
2214	14	-1.6859031003721583
2214	15	-1.7079528223247493
2214	16	-1.5853165864817385
2214	17	-1.3646592570702438
2214	18	-1.3606815613059453
2214	19	-1.4490088009799393
2214	20	-1.512228788541755
2214	21	-1.4378023104174673
2214	22	-1.2981333083913142
2214	23	-1.4188672410450636
2214	24	-1.5745420624406492
2214	25	-1.5224843791308658
2215	1	-2.156631781137534
2215	2	-1.7383734880173591
2215	3	-1.7781611846132712
2215	4	-2.408766373524145
2215	5	-2.0633712248288845
2215	6	-1.82925244159782
2215	7	-1.885634592424232
2215	8	-1.9301561890516545
2215	9	-2.1274408143847374
2215	10	-2.349320223629217
2215	11	-2.165772022663898
2215	12	-2.3365232425555718
2215	13	-2.2642826099375526
2215	14	-2.3030338556722896
2215	15	-2.258948038925297
2215	16	-2.3488945964169403
2215	17	-2.408948601354794
2215	18	-2.56304679431004
2215	19	-2.883885153885071
2215	20	-2.6681970342925396
2215	21	-2.554636648128959
2215	22	-3.5154905335155036
2215	23	-2.836089660394272
2215	24	-2.880528146567986
2215	25	-2.7127398750959024
2216	1	-2.716642883299798
2216	2	-5.340014902158771
2216	3	-3.0552894719159767
2216	4	-5.540956212109332
2216	5	-3.2706034306076006
2216	6	-2.128772419896869
2216	7	-1.7909872608582944
2216	8	-1.881924005645054
2216	9	-2.2181877074572114
2216	10	-2.183305144823443
2216	11	-2.0242781805886594
2216	12	-2.3618575658550114
2216	13	-2.0889034025816002
2216	14	-2.7481788269800482
2216	15	-2.5311320887256237
2216	16	-2.5891053976742526
2216	17	-2.4017189124390583
2216	18	-2.406225256674553
2216	19	-2.534455083524769
2216	20	-2.765408769585669
2216	21	-2.9195922674450436
2216	22	-2.9435991389496934
2216	23	-2.7771146668262574
2216	24	-2.722722934983704
2216	25	-3.009034773134985
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55869.0
3	1.0
4	272.0
5	2301.0
6	4969.0
7	5366.0
8	4218.0
9	3052.0
10	3368.0
11	4354.0
12	4200.0
13	3511.0
14	2557.0
15	2209.0
16	1972.0
17	2078.0
18	2837.0
19	4628.0
20	6424.0
21	8001.0
22	8815.0
23	9385.0
24	10031.0
25	10475.0
26	10848.0
27	11167.0
28	11900.0
29	13280.0
30	16215.0
31	20559.0
32	26315.0
33	34150.0
34	46294.0
35	64617.0
36	97324.0
37	170282.0
38	445896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.8569569958312	17.520348576288388	13.524127248474358	22.09856717940606
2	16.57302068886837	23.16481216732207	37.76556299890548	22.49660414490408
3	18.991480678871426	27.976391576774173	28.78000861113761	24.252119133216794
4	12.388003815670997	18.226604498569124	36.50099403578529	32.88439764997459
5	15.124059711286675	36.518910089809445	31.329256408496096	17.027773790407785
6	30.28292079252059	37.69483570586652	15.458466849621875	16.56377665199102
7	27.02141149466697	33.684112904592176	20.572191898213585	18.72228370252727
8	26.125309180268815	32.55786374490198	19.278225684209023	22.03860139062018
9	27.335354779924735	16.178199937769637	18.984807054676235	37.50163822762939
10	16.70242990954143	28.59113029974263	29.709225668588402	24.997214122127538
11	32.52938034902455	26.00565450795884	21.947015958694095	19.517949184322518
12	24.827447235544668	28.17293349061693	27.271941016868755	19.727678256969654
13	29.196417107722155	24.649057734282493	24.112658173673623	22.041866984321725
14	23.167417183111148	24.4108966070712	26.392349190779797	26.02933701903785
15	23.285081704387586	30.19923636759217	24.74761075077442	21.768071177245822
16	21.555176118657744	30.210290269226824	25.17964058508575	23.054893027029678
17	21.972215286942255	27.711684559688983	25.429789207119164	24.886310946249598
18	24.283074859825085	25.76516599848768	27.520740893980683	22.431018247706554
19	25.910400114139982	25.882310977947796	27.536716513736927	20.670572394175295
20	24.846031413425827	26.18397763699348	27.65299889879135	21.31699205078935
21	23.967661369924574	26.758281490886816	27.765676005588908	21.508381133599702
22	22.742128067871032	27.247483326957493	28.182992827849667	21.827395777321808
23	22.39200951553854	27.277906866844877	28.315681914235324	22.01440170338126
24	21.89459436324338	27.96657322005607	28.758818578042188	21.380013838658364
25	21.75909289109753	28.01820495375196	28.756265309379884	21.46643684577062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10585.0
1	10585.0
2	8730.5
3	6876.0
4	6876.0
5	6876.0
6	8415.5
7	9955.0
8	9955.0
9	9955.0
10	10323.0
11	10691.0
12	10691.0
13	10691.0
14	9928.5
15	9166.0
16	9166.0
17	9166.0
18	14414.0
19	19662.0
20	19662.0
21	19662.0
22	26557.5
23	33453.0
24	33453.0
25	33453.0
26	44321.5
27	55190.0
28	55190.0
29	55190.0
30	64376.5
31	73563.0
32	73563.0
33	73563.0
34	78471.0
35	83379.0
36	83379.0
37	83379.0
38	87772.0
39	92165.0
40	92165.0
41	92165.0
42	100744.5
43	109324.0
44	109324.0
45	109324.0
46	117074.0
47	124824.0
48	124824.0
49	124824.0
50	127951.5
51	131079.0
52	131079.0
53	131079.0
54	123273.0
55	115467.0
56	115467.0
57	115467.0
58	99572.0
59	83677.0
60	83677.0
61	83677.0
62	71683.0
63	59689.0
64	59689.0
65	59689.0
66	49752.0
67	39815.0
68	39815.0
69	39815.0
70	32272.0
71	24729.0
72	24729.0
73	24729.0
74	20162.5
75	15596.0
76	15596.0
77	15596.0
78	13095.5
79	10595.0
80	10595.0
81	10595.0
82	8265.5
83	5936.0
84	5936.0
85	5936.0
86	4423.0
87	2910.0
88	2910.0
89	2910.0
90	2086.5
91	1263.0
92	1263.0
93	1263.0
94	698.0
95	133.0
96	133.0
97	133.0
98	75.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3962858710853825
2	0.6890080903570733
3	0.7025510294404023
4	0.7125533308548869
5	0.7207853134349496
6	0.7217589887938818
7	0.7376033423619593
8	0.7281321365978013
9	0.7170676438826633
10	0.7091012091277639
11	0.7220245366190451
12	0.7257422061713314
13	0.7247685308123992
14	0.7337971568679519
15	0.7309646467328766
16	0.725388142404447
17	0.7311416786163187
18	0.7332660612176253
19	0.735567475702374
20	0.7298139394905022
21	0.728751748189849
22	0.7363641191778639
23	0.7263618177633792
24	0.7297254235487811
25	0.7335316090427886
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1129740.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.83337865874032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.46728492993236	57.748324551605236
2	5.70193086625781	7.27947024143588
3	1.2722823447623	2.436422420221257
4	0.6114071871150549	1.561127459591624
5	0.3582577322424527	1.1434400739827044
6	0.2489246761975279	0.9533821867932675
7	0.18041334164276224	0.8061475206519789
8	0.134749753496288	0.6881225631280378
9	0.10635299056659148	0.6109983646494005
>10	0.7810706310525901	9.687208073235997
>50	0.07856627030871895	3.512554586397702
>100	0.05427169581463761	6.554415828616033
>500	0.002944974775930053	1.371952065299889
>1k	8.414213645514438E-4	1.661900697657082
>5k	5.609475763676292E-4	2.603808542566741
>10k+	1.402368940919073E-4	1.3807248241671335
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	15424	1.3652698851063076	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9564	0.8465664666206383	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7423	0.6570538353957548	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6904	0.6111140616425018	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5196	0.4599288331828562	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4834	0.4278860622798166	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4175	0.3695540566856091	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3739	0.3309611060952078	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2579	0.22828261369872713	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	1717	0.15198187193513552	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1521	0.13463274735779912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.851594172110397E-5	0.0	0.0	8.851594172110397E-5	0.0
6	1.7703188344220794E-4	0.0	0.0	8.851594172110397E-5	0.0
7	1.7703188344220794E-4	0.0	0.0	8.851594172110397E-5	0.0
8	2.655478251633119E-4	0.0	0.0	8.851594172110397E-5	0.0
9	2.655478251633119E-4	0.0	0.0	1.7703188344220794E-4	0.0
10	2.655478251633119E-4	0.0	0.0	2.655478251633119E-4	0.0
11	2.655478251633119E-4	0.0	0.0	4.425797086055199E-4	0.0
12	2.655478251633119E-4	0.0	0.0	4.425797086055199E-4	0.0
13	2.655478251633119E-4	0.0	0.0	4.425797086055199E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCTC	55	1.9600488E-4	13.817632	16
CTAGACC	50	0.0014985367	13.300654	4
AAATCGC	50	0.0014995441	13.299469	14
TGTGCCG	60	4.0933644E-4	12.665596	10
TCTAGAC	85	3.9383776E-6	12.2947235	3
CGACGAC	65	8.0230494E-4	11.691841	14
CAAATCG	65	8.0230494E-4	11.691841	13
GGTTAGA	90	7.3995434E-6	11.6189375	1
GTATCAA	7295	0.0	11.220031	1
GAGTATA	60	0.0058445404	11.090804	1
GGTATCA	3035	0.0	10.868936	1
GTAGGTT	70	0.0014902287	10.857677	4
CTTATAC	70	0.0014902287	10.857677	3
AACACCG	70	0.0014907832	10.857193	5
TATGGCG	70	0.0014913379	10.8567095	16
CGAACCT	105	3.4644563E-6	10.856709	16
GTAGGAC	115	8.8007255E-7	10.73966	3
GTATTAG	125	2.2162567E-7	10.647172	1
ATCGCCA	100	2.397794E-5	10.449583	16
TTGAACA	165	9.258656E-10	10.364147	4
>>END_MODULE