FastQCFastQC Report
Thu 2 Feb 2017
SRR4050268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129740
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT232382.0569334537150143No Hit
TATCAACGCAGAGTACTTTTTTTTT177161.568148423531078No Hit
GTACTTTTTTTTTTTTTTTTTTTTT128901.1409704887850303No Hit
ACGCAGAGTACTTTTTTTTTTTTTT113901.0081965762033742No Hit
GGTATCAACGCAGAGTACTTTTTTT113781.007134384902721No Hit
GAGTACTTTTTTTTTTTTTTTTTTT86270.763627029227964No Hit
GCAGAGTACTTTTTTTTTTTTTTTT56340.49869881565669977No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA48580.4300104448811231No Hit
ATCAACGCAGAGTACTTTTTTTTTT32880.29104041637898986No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT31120.27546161063607555No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT19970.17676633561704463No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT17140.1517163241099722No Hit
CAACGCAGAGTACTTTTTTTTTTTT15770.13958964009418096No Hit
AACGCAGAGTACTTTTTTTTTTTTT13390.11852284596455823No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA12200.10798944889974685No Hit
TCAACGCAGAGTACTTTTTTTTTTT11420.10108520544550073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG456.789155E-414.7694812
GGTATCA27350.014.5827041
TACCGTC551.9724637E-413.8073657
ACGCATC655.4832635E-513.14529610
CACGCTG604.112986E-412.65843310
CTTATAA802.7395605E-511.9269421
GTATCAA65750.011.2176751
ACGGTAT600.00590247211.0761299
ATACCGT700.001496751910.8520076
CGTCAAT700.001498964810.85008419
TCTAAGC803.7316003E-410.6990233
TCTAAAC1105.962136E-610.3748113
CGCCCCT750.002623053510.1442595
CGTCGTA750.00266092810.12674610
CCGGTGT856.6240877E-410.05317513
ACTATTA951.653734E-49.99358
AACTCTC951.6559516E-49.9921737
ACAGCTC1651.0306394E-89.7815168
CTATTAT1201.6129803E-59.54155351
GTATAGT800.00435416639.54155351
Produced by FastQC (version 0.11.5)