##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050268_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1129740 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.10605006461664 31.0 30.0 34.0 26.0 34.0 2 30.118130720342734 31.0 30.0 34.0 26.0 34.0 3 30.287535185086835 31.0 30.0 34.0 26.0 34.0 4 33.78643050613415 37.0 35.0 37.0 28.0 37.0 5 33.67938994812966 37.0 35.0 37.0 28.0 37.0 6 33.65775399649477 37.0 35.0 37.0 28.0 37.0 7 33.47039495813196 37.0 35.0 37.0 28.0 37.0 8 33.38990121620904 37.0 35.0 37.0 27.0 37.0 9 34.871929824561406 39.0 35.0 39.0 27.0 39.0 10 34.80170393187813 39.0 35.0 39.0 27.0 39.0 11 34.76625595269708 39.0 35.0 39.0 27.0 39.0 12 34.7154185918884 39.0 35.0 39.0 27.0 39.0 13 34.63158868412201 39.0 35.0 39.0 27.0 39.0 14 35.69901216209039 40.0 36.0 41.0 27.0 41.0 15 35.663910280241474 40.0 36.0 41.0 27.0 41.0 16 35.654537327172626 40.0 36.0 41.0 27.0 41.0 17 35.70760971550976 40.0 36.0 41.0 27.0 41.0 18 35.68167365942606 40.0 36.0 41.0 27.0 41.0 19 35.70825499672491 40.0 36.0 41.0 27.0 41.0 20 35.672915892152176 40.0 36.0 41.0 27.0 41.0 21 35.57570857011348 40.0 36.0 41.0 27.0 41.0 22 35.48321649229026 40.0 35.0 41.0 27.0 41.0 23 35.34608493989768 40.0 35.0 41.0 26.0 41.0 24 35.255511002531556 40.0 35.0 41.0 25.0 41.0 25 35.00272983164268 39.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.874819586564815 1101 2 0.9503372122026192 1101 3 0.9403508103752038 1101 4 0.8648447268163579 1101 5 0.8893572008580719 1101 6 0.8311379922450186 1101 7 0.900099503898339 1101 8 1.023735055032887 1101 9 1.1533498726629432 1101 10 1.0423119763861664 1101 11 1.097311593927543 1101 12 1.1401828177387259 1101 13 1.029185264419219 1101 14 1.21412674787193 1101 15 1.3610846915547299 1101 16 1.2563684235676007 1101 17 1.1971487241062135 1101 18 1.2764164237015834 1101 19 1.2111496566288622 1101 20 1.3786534622441309 1101 21 1.3314909369052117 1101 22 1.3034306616396236 1101 23 1.3766496856192845 1101 24 1.3602085654386329 1101 25 1.3360112774637969 1102 1 0.8377720585637753 1102 2 0.9244458408893728 1102 3 0.8846526432777502 1102 4 0.7555787139990215 1102 5 1.0291263471952163 1102 6 0.925100803089002 1102 7 0.9896246369696584 1102 8 0.8694652654685129 1102 9 1.1860334874819571 1102 10 1.0610098248205873 1102 11 1.1183005705471203 1102 12 1.0704354467087356 1102 13 1.1765285589228682 1102 14 1.324636431687388 1102 15 1.356201475654622 1102 16 1.1829136442122419 1102 17 1.3001993325165628 1102 18 1.2716940146090607 1102 19 1.2270459259109288 1102 20 1.2232757285283995 1102 21 1.3201754487016473 1102 22 1.3638674660395367 1102 23 1.3867406816182921 1102 24 1.2454588929534154 1102 25 1.4123561534802747 1103 1 0.5706309789104189 1103 2 0.5851950786997975 1103 3 0.6271217009276597 1103 4 0.6093001637236171 1103 5 0.493949206333852 1103 6 0.5435521575671842 1103 7 0.6899558892889743 1103 8 0.6515454427185219 1103 9 0.62452533441828 1103 10 0.8323183897213013 1103 11 0.6909223207817803 1103 12 0.9030638911143711 1103 13 0.9484155875113487 1103 14 0.855048523397528 1103 15 0.9767274774154799 1103 16 0.9715324621089607 1103 17 0.9490072971247727 1103 18 0.989197270741144 1103 19 1.0036907273778226 1103 20 0.9393773926119877 1103 21 1.1361785192887695 1103 22 1.090888996530758 1103 23 1.1534306323579244 1103 24 1.0856655754823592 1103 25 0.9235024938788712 1104 1 0.636279442474347 1104 2 0.593467371577379 1104 3 0.6966426573172093 1104 4 0.6874046528658013 1104 5 0.6935041247384177 1104 6 0.7215616456676202 1104 7 0.6912188544018107 1104 8 0.744425680529794 1104 9 0.6966871139901372 1104 10 0.9795712905541052 1104 11 0.9323265699348724 1104 12 1.029514736220662 1104 13 0.9420810415142924 1104 14 0.9882150333010458 1104 15 1.1482283652636696 1104 16 1.0209857345433164 1104 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-1.7134070768878829 2216 13 -1.4027832949791446 2216 14 -1.683596758922853 2216 15 -1.646982195561833 2216 16 -1.664914756894845 2216 17 -1.6202788686993443 2216 18 -1.7621623081816864 2216 19 -1.7334854219155034 2216 20 -1.7655272110942093 2216 21 -1.8375355409765532 2216 22 -1.845385485016692 2216 23 -1.9172831633891576 2216 24 -2.0386083603761307 2216 25 -1.9820869110447603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28952.0 3 0.0 4 439.0 5 3477.0 6 5140.0 7 4398.0 8 3103.0 9 2503.0 10 2755.0 11 2892.0 12 2525.0 13 1882.0 14 1723.0 15 1689.0 16 1687.0 17 1818.0 18 2577.0 19 4011.0 20 5534.0 21 6404.0 22 6889.0 23 7592.0 24 8211.0 25 8603.0 26 9146.0 27 9943.0 28 11339.0 29 13576.0 30 16888.0 31 21594.0 32 28131.0 33 37035.0 34 49148.0 35 67891.0 36 99983.0 37 172856.0 38 477406.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15495167761488 18.646457588228024 14.514673629428515 22.68391710472858 2 16.024371115112178 24.02960791291124 36.31474960564682 23.631271366329763 3 18.200205356984792 27.98688193743693 29.519358436454407 24.293554269123867 4 12.511673814803503 18.899496753462312 35.0063957013805 33.58243373035369 5 14.272528828779304 37.603137252120376 31.663069903694236 16.461264015406087 6 30.085243323373817 37.83834762910919 15.939863872806265 16.136545174710733 7 25.68262671093734 33.7776629014169 21.528931581980267 19.010778805665492 8 26.09924106028499 32.60298441934119 20.054986395027502 21.24278812534632 9 26.181976545024998 15.692385586405354 20.285304835895722 37.84033303267392 10 16.181471836334378 29.04176086421567 30.807599772686633 23.969167526763314 11 32.49011703764289 24.859213151554098 23.04620074673238 19.604469064070628 12 23.783153625333384 27.764819603028407 29.053742443810453 19.398284327827756 13 29.003120366477088 23.445592758979352 25.911523225706507 21.639763648837054 14 22.480442417004763 22.996105745950164 28.913176459548534 25.61027537749654 15 22.98565990971054 28.60440824997787 26.889528193325663 21.520403646985926 16 21.242761519164823 28.15576520324397 27.445061899965122 23.15641137762608 17 21.659009427698845 26.01239321913867 28.422962864604077 23.905634488558402 18 23.068282505689396 24.017267796904225 31.08229997707423 21.83214972033215 19 24.38910398376963 24.202334354812727 31.48068483377503 19.927876827642613 20 23.353990972720762 24.344973430693635 31.553695846725688 20.74733974985992 21 22.8903579199323 24.62454434563696 31.632105685256363 20.85299204917438 22 21.93033159455902 24.891499951758085 31.965092327942337 21.213076125740557 23 21.361727590748792 24.837392184910843 32.19785219595791 21.603028028382454 24 21.261436543118954 25.589176710524452 32.208208817822594 20.941177928534 25 21.24830811476404 25.31502925224205 32.27785071043689 21.158811922557025 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8873.0 1 8873.0 2 7988.0 3 7103.0 4 7103.0 5 7103.0 6 11488.0 7 15873.0 8 15873.0 9 15873.0 10 14961.5 11 14050.0 12 14050.0 13 14050.0 14 11252.0 15 8454.0 16 8454.0 17 8454.0 18 14520.0 19 20586.0 20 20586.0 21 20586.0 22 29840.5 23 39095.0 24 39095.0 25 39095.0 26 51806.5 27 64518.0 28 64518.0 29 64518.0 30 71687.0 31 78856.0 32 78856.0 33 78856.0 34 82346.0 35 85836.0 36 85836.0 37 85836.0 38 87949.5 39 90063.0 40 90063.0 41 90063.0 42 97357.0 43 104651.0 44 104651.0 45 104651.0 46 110908.5 47 117166.0 48 117166.0 49 117166.0 50 119868.0 51 122570.0 52 122570.0 53 122570.0 54 115832.5 55 109095.0 56 109095.0 57 109095.0 58 95696.5 59 82298.0 60 82298.0 61 82298.0 62 71071.5 63 59845.0 64 59845.0 65 59845.0 66 49941.5 67 40038.0 68 40038.0 69 40038.0 70 32317.5 71 24597.0 72 24597.0 73 24597.0 74 20001.0 75 15405.0 76 15405.0 77 15405.0 78 12921.5 79 10438.0 80 10438.0 81 10438.0 82 8206.0 83 5974.0 84 5974.0 85 5974.0 86 4469.0 87 2964.0 88 2964.0 89 2964.0 90 2116.5 91 1269.0 92 1269.0 93 1269.0 94 691.0 95 113.0 96 113.0 97 113.0 98 61.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4336395984916883 2 0.0037176695522863666 3 0.0 4 0.006638695629082798 5 0.16587887478534882 6 0.044435002743994194 7 0.0020358666595853914 8 5.310956503266238E-4 9 8.851594172110397E-5 10 0.0019473507178642871 11 0.0015932869509798716 12 0.0032750898436808467 13 0.005753536211871758 14 0.011241524598580204 15 0.0035406376688441586 16 0.0012392231840954555 17 0.007966434754899358 18 6.196115920477277E-4 19 5.310956503266238E-4 20 0.0046913449112185104 21 0.004071733319170783 22 0.0015047710092587674 23 0.002478446368190911 24 0.002478446368190911 25 0.007346823162851629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1129740.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.575535394056494 #Duplication Level Percentage of deduplicated Percentage of total 1 90.4210707916698 56.581469156926225 2 5.808432937788672 7.269316017651972 3 1.2553552307633271 2.3566357702423364 4 0.6056074614244543 1.5158484454908256 5 0.35321724056142023 1.105137896927106 6 0.22921903766656343 0.8606102402697366 7 0.17553562949299517 0.7688965197379833 8 0.1439691579075777 0.7207157709038509 9 0.10979578023939299 0.6183476759239366 >10 0.7702881260132483 9.500561682150602 >50 0.07742693444281304 3.4198662259779717 >100 0.04723612328678808 5.1957533721356315 >500 5.691097486014584E-4 0.19373116885891956 >1k 0.0012804969343532815 1.802607985241978 >5k 2.845548743007292E-4 1.2696692091438657 >10k+ 7.113871857518231E-4 6.82083286241707 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 23238 2.0569334537150143 No Hit TATCAACGCAGAGTACTTTTTTTTT 17716 1.568148423531078 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 12890 1.1409704887850303 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11390 1.0081965762033742 No Hit GGTATCAACGCAGAGTACTTTTTTT 11378 1.007134384902721 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 8627 0.763627029227964 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 5634 0.49869881565669977 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4858 0.4300104448811231 No Hit ATCAACGCAGAGTACTTTTTTTTTT 3288 0.29104041637898986 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 3112 0.27546161063607555 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 1997 0.17676633561704463 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 1714 0.1517163241099722 No Hit CAACGCAGAGTACTTTTTTTTTTTT 1577 0.13958964009418096 No Hit AACGCAGAGTACTTTTTTTTTTTTT 1339 0.11852284596455823 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 1220 0.10798944889974685 No Hit TCAACGCAGAGTACTTTTTTTTTTT 1142 0.10108520544550073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.851594172110397E-5 2 8.851594172110397E-5 0.0 0.0 0.0 8.851594172110397E-5 3 8.851594172110397E-5 0.0 0.0 0.0 8.851594172110397E-5 4 8.851594172110397E-5 0.0 0.0 0.0 8.851594172110397E-5 5 8.851594172110397E-5 0.0 0.0 8.851594172110397E-5 8.851594172110397E-5 6 1.7703188344220794E-4 0.0 0.0 8.851594172110397E-5 8.851594172110397E-5 7 1.7703188344220794E-4 0.0 0.0 8.851594172110397E-5 8.851594172110397E-5 8 1.7703188344220794E-4 0.0 0.0 8.851594172110397E-5 8.851594172110397E-5 9 1.7703188344220794E-4 0.0 0.0 1.7703188344220794E-4 8.851594172110397E-5 10 1.7703188344220794E-4 0.0 0.0 2.655478251633119E-4 8.851594172110397E-5 11 1.7703188344220794E-4 0.0 0.0 4.425797086055199E-4 8.851594172110397E-5 12 1.7703188344220794E-4 0.0 0.0 4.425797086055199E-4 1.7703188344220794E-4 13 1.7703188344220794E-4 0.0 0.0 4.425797086055199E-4 2.655478251633119E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGTG 45 6.789155E-4 14.76948 12 GGTATCA 2735 0.0 14.582704 1 TACCGTC 55 1.9724637E-4 13.807365 7 ACGCATC 65 5.4832635E-5 13.145296 10 CACGCTG 60 4.112986E-4 12.658433 10 CTTATAA 80 2.7395605E-5 11.926942 1 GTATCAA 6575 0.0 11.217675 1 ACGGTAT 60 0.005902472 11.076129 9 ATACCGT 70 0.0014967519 10.852007 6 CGTCAAT 70 0.0014989648 10.850084 19 TCTAAGC 80 3.7316003E-4 10.699023 3 TCTAAAC 110 5.962136E-6 10.374811 3 CGCCCCT 75 0.0026230535 10.144259 5 CGTCGTA 75 0.002660928 10.126746 10 CCGGTGT 85 6.6240877E-4 10.053175 13 ACTATTA 95 1.653734E-4 9.9935 8 AACTCTC 95 1.6559516E-4 9.992173 7 ACAGCTC 165 1.0306394E-8 9.781516 8 CTATTAT 120 1.6129803E-5 9.5415535 1 GTATAGT 80 0.0043541663 9.5415535 1 >>END_MODULE