FastQCFastQC Report
Thu 2 Feb 2017
SRR4050267_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050267_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4039704
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT107630.26643041173313686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN89110.22058546863829628No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN68290.16904703908999275No Hit
TATCAACGCAGAGTACTTTTTTTTT64400.15941762069696194No Hit
CTATTGGAGCTGGAATTACCGCGGC40450.10013109871416322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.015.251561
GTATCAA69900.012.9126231
AACCGCG5000.011.5895977
CGGTTCT7850.011.49655912
CGAACGA3300.011.22744216
AGAACCG6050.011.1496245
GAACCGC5700.010.9998916
TGCGTTA951.3620951E-510.99961813
GTATTAC2550.010.8050271
ACGGTAT6500.010.6685949
GCGTTAT2350.010.5117141
CGTTATT2450.010.4703292
GGTTCTA8750.010.42249413
ACCGCGT856.6006515E-410.0583498
CTCGACG951.645872E-49.99965213
CCGTCCA2107.2759576E-129.9517879
ACGAACG3800.09.75002615
CGCGGTC5750.09.74724410
GTATAAA3900.09.7445871
TAGGACG1951.6370905E-109.7443454
Produced by FastQC (version 0.11.5)