##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050267_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4039704
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.844449246776495	31.0	30.0	34.0	26.0	34.0
2	29.89683179757725	31.0	30.0	34.0	26.0	34.0
3	30.033889611714127	31.0	31.0	34.0	26.0	34.0
4	33.43770409911221	37.0	35.0	37.0	28.0	37.0
5	33.53964993474769	37.0	35.0	37.0	28.0	37.0
6	33.61028431786091	37.0	35.0	37.0	29.0	37.0
7	33.53142581733711	37.0	35.0	37.0	28.0	37.0
8	33.45316364763359	37.0	35.0	37.0	28.0	37.0
9	34.91605226521547	39.0	35.0	39.0	28.0	39.0
10	34.8043027409929	39.0	35.0	39.0	27.0	39.0
11	34.83014696126251	39.0	35.0	39.0	27.0	39.0
12	34.7208602412454	39.0	35.0	39.0	27.0	39.0
13	34.70438749967819	39.0	35.0	39.0	27.0	39.0
14	35.53913207502332	40.0	36.0	41.0	25.0	41.0
15	35.65767170069886	40.0	36.0	41.0	27.0	41.0
16	35.68100435081382	40.0	36.0	41.0	27.0	41.0
17	35.67025628610413	40.0	36.0	41.0	27.0	41.0
18	35.49455653186471	40.0	36.0	41.0	26.0	41.0
19	35.62796680152803	40.0	36.0	41.0	27.0	41.0
20	35.59027542612033	40.0	36.0	41.0	27.0	41.0
21	35.5463608224761	40.0	36.0	41.0	26.0	41.0
22	35.469571532963805	40.0	36.0	41.0	26.0	41.0
23	35.35287486409895	40.0	36.0	41.0	25.0	41.0
24	35.28512311793141	40.0	36.0	41.0	25.0	41.0
25	34.998245168457885	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.1478443096651993
1101	2	1.118619245594438
1101	3	1.0961822497476987
1101	4	1.2313495063662714
1101	5	1.146389655407603
1101	6	1.0557315848642972
1101	7	1.096583092679296
1101	8	1.091176472969238
1101	9	1.339300056645925
1101	10	1.3713015954939536
1101	11	1.3161724714738057
1101	12	1.369800374131863
1101	13	1.3907526408647328
1101	14	1.8214579182153088
1101	15	1.7264480102972186
1101	16	1.648432786625179
1101	17	1.637991972566752
1101	18	1.7853110588147416
1101	19	1.662886020674648
1101	20	1.6159609172559257
1101	21	1.6504951421628817
1101	22	1.701325345078608
1101	23	1.6877906239380138
1101	24	1.6826125270200336
1101	25	1.7692972512533487
1102	1	1.073217682768238
1102	2	1.0914801920209172
1102	3	1.024417993497348
1102	4	1.230124616041735
1102	5	1.144839621252757
1102	6	1.1236134685469281
1102	7	1.1342118563131862
1102	8	1.093988104502543
1102	9	1.2802000051475915
1102	10	1.3321416087993256
1102	11	1.2694412397091313
1102	12	1.3146259098769093
1102	13	1.3454078714707762
1102	14	1.7551791464773956
1102	15	1.6112061636863402
1102	16	1.5592842662244948
1102	17	1.5232614487770846
1102	18	1.5915212009025623
1102	19	1.5480633055769104
1102	20	1.5495200989577427
1102	21	1.5788277545737444
1102	22	1.653953451510489
1102	23	1.6239280440306914
1102	24	1.6662482647908021
1102	25	1.6378179054618514
1103	1	0.8371582621679998
1103	2	0.8684187499966178
1103	3	0.8038740498789032
1103	4	0.9957131308419633
1103	5	0.906167132426269
1103	6	0.8619155116191024
1103	7	0.8248247844047256
1103	8	0.8766526084834183
1103	9	0.9908917403448712
1103	10	1.012033425565832
1103	11	1.026982215350131
1103	12	1.1023238328658564
1103	13	1.0512134707996523
1103	14	1.4366058441836458
1103	15	1.2707779947908975
1103	16	1.2152158145338348
1103	17	1.2065904302703814
1103	18	1.3108905019120414
1103	19	1.2439892781213828
1103	20	1.2213605125561031
1103	21	1.1508649396059027
1103	22	1.1897883506529325
1103	23	1.1494055960870213
1103	24	1.1623934490008239
1103	25	1.1475905474995187
1104	1	0.749012317771637
1104	2	0.756822928691463
1104	3	0.684949707253729
1104	4	0.8374700021461692
1104	5	0.7978738916461126
1104	6	0.7927740681585647
1104	7	0.7149984997013021
1104	8	0.8042215082708779
1104	9	0.8225353573490395
1104	10	0.9545029718387568
1104	11	0.9660885197521054
1104	12	1.0196269901992494
1104	13	0.9696230638751828
1104	14	1.3409191874289164
1104	15	1.2816840514521814
1104	16	1.16127906632331
1104	17	1.131912665071468
1104	18	1.2775053972220647
1104	19	1.1657387389712568
1104	20	1.1434069728398057
1104	21	1.1164605882642888
1104	22	1.1827189540731737
1104	23	1.0903694609703933
1104	24	1.186884184325173
1104	25	1.2124726492982845
1105	1	0.7114734741059507
1105	2	0.8440732707419087
1105	3	0.7190731415792193
1105	4	0.8047537929250979
1105	5	0.7740772059128957
1105	6	0.7872412976406622
1105	7	0.7929282174351684
1105	8	0.8038823146263852
1105	9	0.8651626652618276
1105	10	0.9015335121665444
1105	11	0.8355738913966562
1105	12	0.8153639413310998
1105	13	0.8958789138390273
1105	14	1.1947381538581396
1105	15	1.1467533674714474
1105	16	1.199763982528907
1105	17	1.1819373219195342
1105	18	1.2720313825712068
1105	19	1.157808964690389
1105	20	1.1988311024342195
1105	21	1.1266080946476222
1105	22	1.1312020749115206
1105	23	1.108679703493408
1105	24	1.1096385085802751
1105	25	1.0552863031368744
1106	1	0.45311730953809004
1106	2	0.3957306743010811
1106	3	0.4107899525020642
1106	4	0.5523170348935054
1106	5	0.4102816036588095
1106	6	0.3759349679670194
1106	7	0.3358480140186515
1106	8	0.4578458569277828
1106	9	0.5604174533184221
1106	10	0.5955292796105027
1106	11	0.5950172887516203
1106	12	0.7057616549525818
1106	13	0.6740148951284226
1106	14	0.964773900524996
1106	15	0.6827157991641357
1106	16	0.7231822031714046
1106	17	0.682713744591247
1106	18	0.847282410299087
1106	19	0.7654804108462159
1106	20	0.7031687660502826
1106	21	0.7434991851463124
1106	22	0.8620173352537392
1106	23	0.737467262074766
1106	24	0.8037375056802034
1106	25	0.7114401191922184
1107	1	0.47418895918282544
1107	2	0.5329914518847012
1107	3	0.5216796505593493
1107	4	0.5844902185240244
1107	5	0.4845960531936129
1107	6	0.5573929779207631
1107	7	0.5453910331718532
1107	8	0.5450630986643148
1107	9	0.5885023554976101
1107	10	0.634528323277344
1107	11	0.615640416431944
1107	12	0.53250107685475
1107	13	0.4453340826647505
1107	14	0.87101322525276
1107	15	0.7154244052208014
1107	16	0.6215131578007984
1107	17	0.5948384351956406
1107	18	0.6754215503526666
1107	19	0.6679108840556154
1107	20	0.617714576292208
1107	21	0.6522859134338219
1107	22	0.6594737630197613
1107	23	0.6224881053431517
1107	24	0.6901388034508429
1107	25	0.6448368013925219
1108	1	0.38307960848439393
1108	2	0.3485426906222706
1108	3	0.3091871000120854
1108	4	0.5119757920228665
1108	5	0.3458265055181542
1108	6	0.34279962978888534
1108	7	0.3512130789836547
1108	8	0.34615056302634173
1108	9	0.4059436268155068
1108	10	0.3704199423055812
1108	11	0.4054085022496423
1108	12	0.42033116288425276
1108	13	0.40415688224695856
1108	14	0.7213850230263361
1108	15	0.5661854433889957
1108	16	0.5779813592796899
1108	17	0.4215210251756787
1108	18	0.6772910255581905
1108	19	0.6300931013034585
1108	20	0.632708481757355
1108	21	0.6070950413235394
1108	22	0.6526978238695946
1108	23	0.6225580834775784
1108	24	0.6418595491644226
1108	25	0.6084009829702097
1109	1	0.09817686442702822
1109	2	0.16718379945697492
1109	3	0.14380365187250987
1109	4	0.3074708233593242
1109	5	0.15652803685057393
1109	6	0.21196800376147706
1109	7	0.21515946621676818
1109	8	0.31093929400064013
1109	9	0.27814376272781516
1109	10	0.2522134907056923
1109	11	0.12161100352003729
1109	12	0.18902207934456072
1109	13	0.2606393850498989
1109	14	0.5067332630224612
1109	15	0.3019453020724612
1109	16	0.2788918608243307
1109	17	0.2653676660848845
1109	18	0.3669396599823145
1109	19	0.2802822492905861
1109	20	0.17827489730217394
1109	21	0.21884424682133385
1109	22	0.2102186745112462
1109	23	0.25734109766569446
1109	24	0.269893982970693
1109	25	0.18315643156147843
1110	1	-0.09717081799352556
1110	2	-0.13358283366335044
1110	3	-0.13054097156159372
1110	4	-0.01593129514141367
1110	5	-0.08575970164570634
1110	6	-0.13993611766060354
1110	7	-0.22992775490240547
1110	8	-0.16148650462727687
1110	9	-0.2174618593305624
1110	10	-0.27326142575709866
1110	11	-0.20648065079743105
1110	12	-0.24136014607471878
1110	13	-0.27945236368775284
1110	14	-0.039278233108966276
1110	15	-0.14003747629265462
1110	16	-0.20887100838134387
1110	17	-0.4370557473905805
1110	18	-0.11185945368138306
1110	19	-0.18388282601046058
1110	20	-0.42671475439731665
1110	21	-0.2723006851278811
1110	22	-0.3431464570627156
1110	23	-0.28899138917235945
1110	24	-0.32231756526247324
1110	25	-0.3328067889327784
1111	1	-0.5058945959236851
1111	2	-0.49962001402556666
1111	3	-0.6266213715883886
1111	4	-0.3883852342668277
1111	5	-0.5024690428752336
1111	6	-0.5108095272533149
1111	7	-0.5203071607468388
1111	8	-0.5252389034952003
1111	9	-0.6903126077348816
1111	10	-0.5601683300810336
1111	11	-0.6493946956000158
1111	12	-0.6136827652389556
1111	13	-0.6200192677125926
1111	14	-0.3906631941240164
1111	15	-0.47506566893620317
1111	16	-0.5129354319721102
1111	17	-0.6200980309137449
1111	18	-0.5558803824019876
1111	19	-0.6178967135388049
1111	20	-0.610380984717132
1111	21	-0.6383210965327919
1111	22	-0.6457745907007748
1111	23	-0.6176424668145799
1111	24	-0.7386449370766286
1111	25	-0.7698857232907628
1112	1	-0.5724000952422301
1112	2	-0.5863593573771091
1112	3	-0.6000898150317191
1112	4	-0.44674254843366157
1112	5	-0.5310098930456064
1112	6	-0.5900734355059996
1112	7	-0.637683438063668
1112	8	-0.5918241286778709
1112	9	-0.7294109536250346
1112	10	-0.6869047013734644
1112	11	-0.6814077837611734
1112	12	-0.6232805230247109
1112	13	-0.7253073631777553
1112	14	-0.6580369989298731
1112	15	-0.6853210641822258
1112	16	-0.852395041991528
1112	17	-0.8508722396976154
1112	18	-0.7804026705746594
1112	19	-0.7385677378799187
1112	20	-0.6863816830753322
1112	21	-0.6585563281995022
1112	22	-0.659119590249162
1112	23	-0.843133575614651
1112	24	-0.7440278205391095
1112	25	-0.727884447676935
1113	1	-0.7771607864974364
1113	2	-0.9303317892373038
1113	3	-0.8467567501164233
1113	4	-0.6935156452633748
1113	5	-0.763463409912589
1113	6	-0.9147176992317085
1113	7	-0.966648971017726
1113	8	-0.9727568604654877
1113	9	-0.9684918042723396
1113	10	-1.0482321073293122
1113	11	-1.0663157344561611
1113	12	-1.0103155661246959
1113	13	-0.9910317780565663
1113	14	-0.9745668808894195
1113	15	-1.0950885434402977
1113	16	-1.0682800938150976
1113	17	-1.1176743650541425
1113	18	-1.159673721922303
1113	19	-1.0425210091926118
1113	20	-1.2232128168675018
1113	21	-1.134463759979731
1113	22	-1.201371643305599
1113	23	-1.6094091113919191
1113	24	-1.485655106502442
1113	25	-1.5476678335165346
1114	1	-2.5810195976311725
1114	2	-2.712975498840205
1114	3	-2.329572091559278
1114	4	-5.770828948051189
1114	5	-3.252482588610679
1114	6	-2.6377906766964685
1114	7	-2.4123130427307444
1114	8	-2.6154739914465672
1114	9	-3.1903285512211994
1114	10	-2.937652000359609
1114	11	-2.6463381548906213
1114	12	-2.75650183768429
1114	13	-2.7847980013937175
1114	14	-2.710802088462337
1114	15	-3.409907048477912
1114	16	-3.396639594604636
1114	17	-3.0656637300972776
1114	18	-3.0642345201165924
1114	19	-3.0690718003799446
1114	20	-3.366317888536095
1114	21	-3.3456261320878014
1114	22	-3.4928933930493287
1114	23	-3.5309008707445493
1114	24	-3.5054697888696715
1114	25	-3.452777606814795
1115	1	-2.844810918704386
1115	2	-2.2589722870436617
1115	3	-1.8460014551031065
1115	4	-1.8671639277560814
1115	5	-1.8252105219833368
1115	6	-1.7631246793132291
1115	7	-1.8177970792857607
1115	8	-1.8039905272133119
1115	9	-2.1313652577115008
1115	10	-1.9968885890686252
1115	11	-2.02919648495142
1115	12	-2.4637799230378263
1115	13	-2.568236252006926
1115	14	-2.8195603100594155
1115	15	-2.644504833630897
1115	16	-2.600083636784369
1115	17	-2.588025720293423
1115	18	-2.455787999785649
1115	19	-2.4605018700285086
1115	20	-2.5732695977065845
1115	21	-2.509935809421556
1115	22	-2.5843326395466235
1115	23	-2.6171349242109017
1115	24	-2.850412214446486
1115	25	-3.0265237783953296
1116	1	-1.554129081810082
1116	2	-1.4755578867226156
1116	3	-1.5976965866444068
1116	4	-1.4588546902655608
1116	5	-1.7236174290432373
1116	6	-1.7139249807255759
1116	7	-1.7998069942891206
1116	8	-1.751250876332982
1116	9	-2.0568128732351383
1116	10	-2.4555685391044264
1116	11	-2.065550780184928
1116	12	-2.1450515420692042
1116	13	-2.0491052512619987
1116	14	-2.9453731719493135
1116	15	-2.7817807129572216
1116	16	-2.6946481231268393
1116	17	-2.427368714910756
1116	18	-2.4129585999173457
1116	19	-2.192116254664846
1116	20	-2.2376578423121316
1116	21	-2.4656260431099994
1116	22	-2.4069595111262316
1116	23	-2.778711694886205
1116	24	-2.891005409278513
1116	25	-2.8497083175768836
1201	1	1.0459245146070977
1201	2	0.9981108894736934
1201	3	1.0169412703001584
1201	4	1.130741826557987
1201	5	1.0246770004476886
1201	6	1.006631851893097
1201	7	0.9283553676718554
1201	8	1.0450118585943429
1201	9	1.1675500497547446
1201	10	1.1592525009947892
1201	11	1.1307140092289174
1201	12	1.188128954587505
1201	13	1.2002565353400314
1201	14	1.6043186679127075
1201	15	1.420738241607033
1201	16	1.426676830640794
1201	17	1.3478938805683
1201	18	1.4479011718754293
1201	19	1.4291140129551323
1201	20	1.4026797149598949
1201	21	1.3789707472170676
1201	22	1.4465127380254614
1201	23	1.460151485813327
1201	24	1.4491145265085166
1201	25	1.4625199605297112
1202	1	0.9898005068695213
1202	2	0.9571776462185646
1202	3	0.9189598631072222
1202	4	0.9825772605370204
1202	5	0.9428973435315484
1202	6	0.8778600322222374
1202	7	0.906070554038827
1202	8	0.9341750871950651
1202	9	1.0789730652341518
1202	10	1.1658980720657581
1202	11	1.1038843098403106
1202	12	1.1422854757124483
1202	13	1.151541363744613
1202	14	1.5716147104765312
1202	15	1.409897616019677
1202	16	1.4127761495021858
1202	17	1.3338713935031734
1202	18	1.5156413289667015
1202	19	1.350059080248073
1202	20	1.3880958068233653
1202	21	1.4671476432441466
1202	22	1.4476936816291825
1202	23	1.4310261505032074
1202	24	1.3848599816652083
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1203	1	0.7970467465078883
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2211	9	-0.15309039736585817
2211	10	-0.10367510734596408
2211	11	-0.07326000847314162
2211	12	-0.08454246206569138
2211	13	-0.1718572644901002
2211	14	0.07269184637046777
2211	15	-0.1312584525497087
2211	16	-0.12735832729807584
2211	17	-0.2568456391354417
2211	18	-0.155797330977272
2211	19	-0.09403543075384135
2211	20	-0.1795511263470715
2211	21	-0.22700081594382482
2211	22	-0.2996710142741037
2211	23	-0.16303943444980717
2211	24	-0.13856283390794744
2211	25	-0.22347056573407542
2212	1	-0.3145703257445618
2212	2	-0.37368195499688284
2212	3	-0.4845460579317944
2212	4	-0.31572749620173823
2212	5	-0.34937530897169466
2212	6	-0.4549794595354726
2212	7	-0.5042385929301076
2212	8	-0.4279648509404268
2212	9	-0.5559606280480551
2212	10	-0.758917828340337
2212	11	-0.5957281983209199
2212	12	-0.5808965242800639
2212	13	-0.5370135217424092
2212	14	-0.6086802191353726
2212	15	-0.6971187600371849
2212	16	-0.6543956631419121
2212	17	-0.8443498052197356
2212	18	-0.6501785478742903
2212	19	-0.7101840052947779
2212	20	-0.7048412502089647
2212	21	-0.7145343067205445
2212	22	-0.8584193197317305
2212	23	-0.9518880695657117
2212	24	-0.7002696541388929
2212	25	-0.7849911156585065
2213	1	-0.9152125653608003
2213	2	-0.7535621467090401
2213	3	-0.742942724686209
2213	4	-0.6911514837782136
2213	5	-0.7264262310165392
2213	6	-0.820875612467816
2213	7	-0.9540757433961105
2213	8	-0.7203979477637432
2213	9	-0.7736974747093726
2213	10	-1.000874867753275
2213	11	-0.9278259962938478
2213	12	-0.8820203434173948
2213	13	-1.1111421208592773
2213	14	-0.8275379192779297
2213	15	-1.0491889862673887
2213	16	-1.087939401971127
2213	17	-1.1179820977807395
2213	18	-1.060252725556488
2213	19	-1.1461940267656203
2213	20	-1.0961920555174842
2213	21	-1.1363315162532999
2213	22	-1.2025490123024554
2213	23	-1.2499159631011025
2213	24	-1.1397904344869616
2213	25	-1.1945240716490133
2214	1	-1.3164487191672372
2214	2	-1.1804268319816025
2214	3	-1.0657156255986315
2214	4	-0.9799238458690027
2214	5	-1.0550738764189447
2214	6	-1.3047510109575313
2214	7	-1.2680335488298695
2214	8	-1.2585397677364156
2214	9	-1.255799320934706
2214	10	-1.4518566616718687
2214	11	-1.3739696485248558
2214	12	-1.3381241698453366
2214	13	-1.5413268641974796
2214	14	-1.2500992714686987
2214	15	-1.4883731762212093
2214	16	-1.4204871744004066
2214	17	-1.473144202666937
2214	18	-1.4317201837746438
2214	19	-1.515363839316592
2214	20	-1.5786097726877557
2214	21	-1.5792874162840178
2214	22	-1.5155440215828975
2214	23	-1.442564142360574
2214	24	-1.5155766573340586
2214	25	-1.5135600558656463
2215	1	-1.6710421442593635
2215	2	-1.7934417612201408
2215	3	-1.702384792161883
2215	4	-1.859120569745528
2215	5	-1.6303443238644633
2215	6	-1.5084841299112384
2215	7	-1.7160919924554001
2215	8	-1.880313687160612
2215	9	-2.2537285432870178
2215	10	-1.9464239727363406
2215	11	-2.06971910493224
2215	12	-2.1606960778419477
2215	13	-2.09419697928265
2215	14	-2.8906504716888293
2215	15	-2.5312584525497073
2215	16	-2.2407616954184206
2215	17	-2.260755146818532
2215	18	-2.138023699996097
2215	19	-2.31804538306568
2215	20	-2.532878930618274
2215	21	-2.3589610988475087
2215	22	-2.583989211799846
2215	23	-2.23736615594634
2215	24	-2.361632845657205
2215	25	-2.3354893645453245
2216	1	-1.9097418036726488
2216	2	-1.7664985335749321
2216	3	-1.7907355675037238
2216	4	-1.7320938417223282
2216	5	-2.2536119544292923
2216	6	-1.8796606950165113
2216	7	-2.1190632361895787
2216	8	-2.567448570563002
2216	9	-2.47601919308854
2216	10	-2.4978849659430438
2216	11	-2.485841789904015
2216	12	-2.6480019131223074
2216	13	-2.5846471174402126
2216	14	-2.4681264422819496
2216	15	-2.5365158154655347
2216	16	-2.669562477213759
2216	17	-2.6534403029800444
2216	18	-2.9466300339080505
2216	19	-2.836140994949602
2216	20	-2.855276524121571
2216	21	-2.9918175756583736
2216	22	-3.1933739167432265
2216	23	-2.865214980750956
2216	24	-3.0397241376477524
2216	25	-3.0892230431189134
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	152427.0
3	10.0
4	1060.0
5	6638.0
6	8922.0
7	8637.0
8	7671.0
9	6913.0
10	6550.0
11	6630.0
12	6187.0
13	5878.0
14	5980.0
15	5881.0
16	6140.0
17	6710.0
18	7351.0
19	8973.0
20	11671.0
21	14954.0
22	18376.0
23	21994.0
24	25827.0
25	29704.0
26	33482.0
27	38023.0
28	42525.0
29	51362.0
30	63732.0
31	80668.0
32	103936.0
33	135594.0
34	182960.0
35	251477.0
36	365759.0
37	631914.0
38	1677188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87940671948585	18.011162589446847	12.631500077159613	25.477930613907684
2	16.389148760443824	20.837050776894834	37.941518311497894	24.832282151163444
3	18.097301202718103	25.09698355060323	29.22725828854023	27.578456958138435
4	12.489345358388384	16.862002801040187	37.91390526344902	32.734746577122415
5	15.277289417909648	35.66050689469992	33.58164304057076	15.480560646819667
6	31.98342122840423	37.12996839765126	16.95991885075118	13.926691523193332
7	28.520822959610143	31.11907613677315	22.131606348294923	18.228494555321788
8	26.484089385530552	32.782270424374424	20.52330956730206	20.21033062279296
9	27.086050534585727	15.129825113991629	19.45233682214914	38.33178752927351
10	15.998211816513836	26.62489586995899	31.63699277176165	25.73989954176552
11	35.14725514398062	22.547116138693283	23.359890869136915	18.945737848189182
12	24.607409301362377	24.332212660241673	28.930884848285487	22.129493190110463
13	28.934957335796017	20.76947574437461	25.184539315807807	25.11102760402156
14	23.313150804331798	20.80246545966854	26.16799980663843	29.716383929361236
15	24.490382283995427	27.487995765718015	23.143819809771294	24.877802140515264
16	24.737166083920442	26.835675532882586	24.109943403420715	24.317214979776256
17	24.167373180198084	25.977792298479162	25.289720390105124	24.56511413121763
18	25.099965162334055	24.929988252878594	26.39090253477661	23.57914405001074
19	26.200118714829735	25.24858097901359	25.408437325098713	23.142862981057956
20	25.229318358701835	25.221318663736938	25.354497386143283	24.194865591417948
21	25.67284523930267	25.12794532161331	25.28145229558964	23.91775714349438
22	24.68912565929732	25.067540205766804	25.67330252355279	24.57003161138309
23	23.947189577275875	24.632054342589885	25.933947845009875	25.486808235124357
24	24.254131680951712	25.721182782380236	25.64347505564526	24.38121048102279
25	24.433382689272385	25.172376157044585	25.660486625304696	24.733754528378334
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15097.0
1	15097.0
2	14126.5
3	13156.0
4	13156.0
5	13156.0
6	10347.0
7	7538.0
8	7538.0
9	7538.0
10	9764.5
11	11991.0
12	11991.0
13	11991.0
14	16279.5
15	20568.0
16	20568.0
17	20568.0
18	33969.5
19	47371.0
20	47371.0
21	47371.0
22	68090.0
23	88809.0
24	88809.0
25	88809.0
26	121169.0
27	153529.0
28	153529.0
29	153529.0
30	183128.0
31	212727.0
32	212727.0
33	212727.0
34	252705.5
35	292684.0
36	292684.0
37	292684.0
38	321961.0
39	351238.0
40	351238.0
41	351238.0
42	385868.5
43	420499.0
44	420499.0
45	420499.0
46	448397.0
47	476295.0
48	476295.0
49	476295.0
50	481498.0
51	486701.0
52	486701.0
53	486701.0
54	456141.0
55	425581.0
56	425581.0
57	425581.0
58	388593.0
59	351605.0
60	351605.0
61	351605.0
62	307277.5
63	262950.0
64	262950.0
65	262950.0
66	217218.5
67	171487.0
68	171487.0
69	171487.0
70	134102.5
71	96718.0
72	96718.0
73	96718.0
74	77370.0
75	58022.0
76	58022.0
77	58022.0
78	48815.5
79	39609.0
80	39609.0
81	39609.0
82	29973.0
83	20337.0
84	20337.0
85	20337.0
86	15376.0
87	10415.0
88	10415.0
89	10415.0
90	7361.0
91	4307.0
92	4307.0
93	4307.0
94	2340.5
95	374.0
96	374.0
97	374.0
98	235.0
99	96.0
100	96.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22521452066785091
2	0.6428441291738206
3	0.6406162431702916
4	0.6484385984715713
5	0.6643803605412674
6	0.6768812764499578
7	0.6723759958650436
8	0.6677221895465608
9	0.6606919714909805
10	0.6607662343577648
11	0.6660636521883782
12	0.6668062808562212
13	0.6618801773595293
14	0.6559143937278573
15	0.662053457382026
16	0.6706927042179328
17	0.6694054811936716
18	0.6635634690066401
19	0.6615831258923921
20	0.6696282697940246
21	0.6648259377419732
22	0.6661131607662344
23	0.6705194241954361
24	0.6741335503789386
25	0.6603949200238433
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4039704.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.09095609401946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.88824281247233	46.45777170961923
2	8.14823342683545	8.81491473069556
3	2.246884098809403	3.6460832741114975
4	1.01813374838792	2.202873115275618
5	0.5779438844974554	1.5630768640579458
6	0.40054771504367964	1.2999605320792498
7	0.2564550680363557	0.9710329877670305
8	0.1913256750161715	0.8279190949566697
9	0.14952788337422995	0.7279295556984423
>10	0.9369141144752127	9.598096554712386
>50	0.09244353672105746	3.4633361253828565
>100	0.07396277806002213	8.194817403916343
>500	0.012289697248606374	4.6541330457724355
>1k	0.006910056158907131	6.751668698315048
>5k	1.3912864749477443E-4	0.5563928088955808
>10k+	4.6376215831591475E-5	0.2699934987440548
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10763	0.26643041173313686	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	8911	0.22058546863829628	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	6829	0.16904703908999275	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6440	0.15941762069696194	No Hit
CTATTGGAGCTGGAATTACCGCGGC	4045	0.10013109871416322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4754288928099684E-5
2	0.0	0.0	0.0	0.0	2.4754288928099684E-5
3	0.0	0.0	0.0	0.0	2.4754288928099684E-5
4	0.0	0.0	0.0	2.4754288928099684E-5	2.4754288928099684E-5
5	0.0	0.0	0.0	2.4754288928099684E-5	2.4754288928099684E-5
6	0.0	0.0	0.0	2.4754288928099684E-5	2.4754288928099684E-5
7	0.0	0.0	0.0	2.4754288928099684E-5	2.4754288928099684E-5
8	0.0	0.0	0.0	2.4754288928099684E-5	2.4754288928099684E-5
9	0.0	0.0	0.0	2.4754288928099684E-5	2.4754288928099684E-5
10	0.0	0.0	0.0	2.4754288928099684E-5	2.4754288928099684E-5
11	0.0	2.4754288928099684E-5	0.0	4.950857785619937E-5	2.4754288928099684E-5
12	0.0	2.4754288928099684E-5	0.0	4.950857785619937E-5	7.426286678429904E-5
13	0.0	2.4754288928099684E-5	0.0	9.901715571239874E-5	9.901715571239874E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2280	0.0	15.25156	1
GTATCAA	6990	0.0	12.912623	1
AACCGCG	500	0.0	11.589597	7
CGGTTCT	785	0.0	11.496559	12
CGAACGA	330	0.0	11.227442	16
AGAACCG	605	0.0	11.149624	5
GAACCGC	570	0.0	10.999891	6
TGCGTTA	95	1.3620951E-5	10.999618	13
GTATTAC	255	0.0	10.805027	1
ACGGTAT	650	0.0	10.668594	9
GCGTTAT	235	0.0	10.511714	1
CGTTATT	245	0.0	10.470329	2
GGTTCTA	875	0.0	10.422494	13
ACCGCGT	85	6.6006515E-4	10.058349	8
CTCGACG	95	1.645872E-4	9.999652	13
CCGTCCA	210	7.2759576E-12	9.951787	9
ACGAACG	380	0.0	9.750026	15
CGCGGTC	575	0.0	9.747244	10
GTATAAA	390	0.0	9.744587	1
TAGGACG	195	1.6370905E-10	9.744345	4
>>END_MODULE