FastQCFastQC Report
Thu 2 Feb 2017
SRR4050267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4039704
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT164730.40777740151258607No Hit
TATCAACGCAGAGTACTTTTTTTTT113320.2805156021332256No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63330.15676891178165528No Hit
GGTATCAACGCAGAGTACTTTTTTT62070.15364987137671474No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55280.13684170919453503No Hit
CTATTGGAGCTGGAATTACCGCGGC43360.10733459679224022No Hit
GAGTACTTTTTTTTTTTTTTTTTTT42340.10480965932157404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.016.2304761
GTATCAA71900.013.1089981
ATTAACG550.003068513212.0916423
CGTCGTA5350.011.89555910
CGAACGA3100.011.02855616
CCGTCGT5800.010.9726299
CGAACGT3300.010.9414124
CGAGCCG7500.010.89143115
TCGAACG3500.010.8578013
AACGTCT3500.010.5828176
CCGTCAA4250.010.50053718
ACCGTCG6250.010.4856488
ATACCGT6000.010.2888516
AGAACCG5000.010.2618935
TACCGTC6000.010.1310627
AACCGCG4450.010.0314597
GAACGTC3800.010.0018455
GTCGTAG6050.09.89107911
GGTAGCG1252.5777335E-69.87851911
GCTCGTA3850.09.8687729
Produced by FastQC (version 0.11.5)