##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050267_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4039704
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.74039409818145	31.0	31.0	34.0	27.0	34.0
2	30.644147937571663	31.0	31.0	34.0	27.0	34.0
3	30.9067471280074	31.0	31.0	34.0	27.0	34.0
4	34.55283010834457	37.0	35.0	37.0	31.0	37.0
5	34.48527911946024	37.0	35.0	37.0	31.0	37.0
6	34.44509597732903	37.0	35.0	37.0	31.0	37.0
7	34.381594295027554	37.0	35.0	37.0	30.0	37.0
8	34.35180968704638	37.0	35.0	37.0	30.0	37.0
9	35.935121979234125	39.0	37.0	39.0	31.0	39.0
10	35.82321972104887	39.0	37.0	39.0	30.0	39.0
11	35.81981872929304	39.0	37.0	39.0	30.0	39.0
12	35.744958789059794	39.0	37.0	39.0	30.0	39.0
13	35.7427554098023	39.0	37.0	39.0	30.0	39.0
14	36.88458263278696	40.0	37.0	41.0	31.0	41.0
15	36.852806294718626	40.0	37.0	41.0	31.0	41.0
16	36.83546269726693	40.0	37.0	41.0	31.0	41.0
17	36.81761460740688	40.0	37.0	41.0	31.0	41.0
18	36.773503207165675	40.0	37.0	41.0	30.0	41.0
19	36.8288216166333	40.0	37.0	41.0	30.0	41.0
20	36.77843401397726	40.0	37.0	41.0	30.0	41.0
21	36.72363445440557	40.0	37.0	41.0	30.0	41.0
22	36.6620655869836	40.0	37.0	41.0	30.0	41.0
23	36.55970883015191	40.0	37.0	41.0	30.0	41.0
24	36.50130133296895	40.0	37.0	41.0	30.0	41.0
25	36.28189169305474	40.0	36.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7937436881058311
1101	2	0.9060770643528855
1101	3	0.8943951023656425
1101	4	0.8311484813421828
1101	5	0.9156219172497018
1101	6	0.9473285907043376
1101	7	0.8894821505521548
1101	8	0.913225972056928
1101	9	1.0461563699418406
1101	10	1.0752665029695834
1101	11	1.0446111374993166
1101	12	1.0800030620567185
1101	13	1.0632874876683616
1101	14	1.2047902954017218
1101	15	1.2446120089879429
1101	16	1.160973404809532
1101	17	1.2555797380984686
1101	18	1.3004647237253621
1101	19	1.2366612516262592
1101	20	1.3108625625722112
1101	21	1.2866067857364598
1101	22	1.3258984911185223
1101	23	1.324848870123084
1101	24	1.2946764631138663
1101	25	1.328953955868009
1102	1	0.5907098212898703
1102	2	0.7828423669067917
1102	3	0.7090602042204317
1102	4	0.6852600408239127
1102	5	0.771974030562582
1102	6	0.8188100772322429
1102	7	0.781455324508407
1102	8	0.8436761035752838
1102	9	0.9198258748128296
1102	10	0.9345296924105853
1102	11	0.8803265993306582
1102	12	1.01514803593912
1102	13	0.9966952993768388
1102	14	1.077617707239881
1102	15	1.1436320899495342
1102	16	1.0969497951578333
1102	17	1.0589133757986957
1102	18	1.053586094328118
1102	19	1.0335869494314238
1102	20	1.0983520393750155
1102	21	1.1110653499124226
1102	22	1.1601892244075103
1102	23	1.1589109215166218
1102	24	1.171228302116937
1102	25	1.165611124796314
1103	1	0.5706917113241019
1103	2	0.7263066357240895
1103	3	0.6016462949024728
1103	4	0.5892223533418033
1103	5	0.6476192435755692
1103	6	0.6601753165887558
1103	7	0.6770185052562141
1103	8	0.7252668670999043
1103	9	0.7661989985185258
1103	10	0.8505124858501034
1103	11	0.8532527124928606
1103	12	0.82072698216529
1103	13	0.8306403858209919
1103	14	0.9062284971820844
1103	15	1.0033808991022397
1103	16	1.0235423198655198
1103	17	0.9632141110970522
1103	18	1.007041305586526
1103	19	0.9555085635670437
1103	20	0.9596506703019116
1103	21	0.9593077845291234
1103	22	0.9041090856354899
1103	23	1.0024884025750112
1103	24	1.098543396491003
1103	25	1.1020209369446263
1104	1	0.4633460180817899
1104	2	0.615548911821886
1104	3	0.5712484059420468
1104	4	0.5356172019382512
1104	5	0.555554427824049
1104	6	0.5311071107275183
1104	7	0.5691398764277125
1104	8	0.5678752233518054
1104	9	0.6959206958885318
1104	10	0.670214351103013
1104	11	0.7606879507862132
1104	12	0.7300496787593715
1104	13	0.6746430873224014
1104	14	0.7644914382073864
1104	15	0.8103465715824214
1104	16	0.8072516319324592
1104	17	0.6356366284402597
1104	18	0.7308580197722776
1104	19	0.7505689367561388
1104	20	0.8302310800387005
1104	21	0.7671811201759624
1104	22	0.8017348710484242
1104	23	0.8317080082725496
1104	24	0.8824175330407442
1104	25	0.9569445976398043
1105	1	0.44293157922947657
1105	2	0.5625100642685936
1105	3	0.46336209038669907
1105	4	0.48179134973373294
1105	5	0.48745116499147656
1105	6	0.5345118586021229
1105	7	0.4790085530889101
1105	8	0.4664276672777987
1105	9	0.5312765072826124
1105	10	0.5696594129830999
1105	11	0.5929644835220955
1105	12	0.6180495395123842
1105	13	0.6329206492136805
1105	14	0.7718847228270533
1105	15	0.8040414927429396
1105	16	0.7624543034483509
1105	17	0.7897545635700141
1105	18	0.7674026081419285
1105	19	0.7295157818694307
1105	20	0.7910358215988822
1105	21	0.8258773772593528
1105	22	0.8448975494630417
1105	23	0.8750311093477166
1105	24	0.9091433533668933
1105	25	0.9370566643690381
1106	1	0.2498565541690283
1106	2	0.4357243706039853
1106	3	0.3599173142445373
1106	4	0.18618595643126667
1106	5	0.3576777533856017
1106	6	0.41822156301942925
1106	7	0.3591979945029493
1106	8	0.28217596091933217
1106	9	0.3342985996061927
1106	10	0.37416010265698674
1106	11	0.33092024819906385
1106	12	0.38430155351372264
1106	13	0.3357081190001594
1106	14	0.3724871378011869
1106	15	0.422886715303612
1106	16	0.48304626820664254
1106	17	0.45348826166495115
1106	18	0.402514251449297
1106	19	0.4517965591340882
1106	20	0.49094771622267785
1106	21	0.5082404265697775
1106	22	0.4685594739373897
1106	23	0.5352912122040365
1106	24	0.5130442123116268
1106	25	0.4954411491227191
1107	1	0.3753955082641802
1107	2	0.5550219023740226
1107	3	0.4893960056520079
1107	4	0.4896857119969624
1107	5	0.4170011241303726
1107	6	0.4600194008674521
1107	7	0.46391476320988545
1107	8	0.49289213630586914
1107	9	0.5645043439407544
1107	10	0.48173634603992355
1107	11	0.5382918260117933
1107	12	0.5527034112144307
1107	13	0.5447509253970608
1107	14	0.5600207956770618
1107	15	0.586117460430124
1107	16	0.6691827764176708
1107	17	0.6087915870844895
1107	18	0.6344580050184234
1107	19	0.6143130509581667
1107	20	0.6864444315564526
1107	21	0.6095227006735229
1107	22	0.5286684805067239
1107	23	0.5417138198514451
1107	24	0.5159477793401379
1107	25	0.5623928179112383
1108	1	0.18273194735309417
1108	2	0.32187993605074183
1108	3	0.26673627588804294
1108	4	0.24750068108822632
1108	5	0.34982538557034104
1108	6	0.27588824066320683
1108	7	0.3452811641534481
1108	8	0.3962309965967066
1108	9	0.40161133988534203
1108	10	0.34513783364631934
1108	11	0.44001651708013156
1108	12	0.4759019414659633
1108	13	0.4679828873389269
1108	14	0.5319594080335008
1108	15	0.5421711920089507
1108	16	0.5313662395670207
1108	17	0.5297501421709825
1108	18	0.5775764757158584
1108	19	0.5360001469486448
1108	20	0.5271997385777354
1108	21	0.5715897758574542
1108	22	0.579278141642277
1108	23	0.5921811886528232
1108	24	0.5363229929603364
1108	25	0.6105769164703574
1109	1	0.05862793007569422
1109	2	0.15475528793052362
1109	3	0.15260573151287815
1109	4	0.14778072295082723
1109	5	0.24017646883578436
1109	6	0.22343829026917916
1109	7	0.2036526261988243
1109	8	0.2576855405847027
1109	9	0.2918451634977899
1109	10	0.2945449313337889
1109	11	0.24306244495384988
1109	12	0.2873308254525071
1109	13	0.2879890679208046
1109	14	0.3638383553382454
1109	15	0.409273709008076
1109	16	0.41755147957120897
1109	17	0.27089698795689543
1109	18	0.3503047841924314
1109	19	0.26780589519227505
1109	20	0.3511150111686163
1109	21	0.30514322730409305
1109	22	0.30775025743795226
1109	23	0.36043976685684953
1109	24	0.27595882259002025
1109	25	0.3388921483278793
1110	1	-0.19201988149953664
1110	2	-0.10976276338855229
1110	3	-0.1609838925810294
1110	4	-0.1066384924288144
1110	5	-0.1446701059487907
1110	6	-0.22149417085928036
1110	7	-0.20215892666055169
1110	8	-0.11282419189902271
1110	9	-0.11897558869359415
1110	10	-0.20480763628850696
1110	11	-0.1999553530612772
1110	12	-0.11042581353779468
1110	13	-0.22175872590798207
1110	14	-0.24053931895231528
1110	15	-0.2124267511102076
1110	16	-0.20572990243692857
1110	17	-0.22953065205420842
1110	18	-0.19493736755634927
1110	19	-0.1940410982263785
1110	20	-0.1594254002910347
1110	21	-0.2758182412975998
1110	22	-0.31326579854373904
1110	23	-0.16189785886609087
1110	24	-0.25559643141923516
1110	25	-0.11750250604874424
1111	1	-0.2745654941695399
1111	2	-0.21425619796568185
1111	3	-0.21518041042519087
1111	4	-0.15964742298259438
1111	5	-0.18960440747481755
1111	6	-0.4123101713293451
1111	7	-0.2931554301338508
1111	8	-0.24446940005222473
1111	9	-0.2694195944019384
1111	10	-0.3125722564079112
1111	11	-0.30587857353643244
1111	12	-0.338141661211246
1111	13	-0.37410810992091115
1111	14	-0.4057189463416577
1111	15	-0.36151676306793235
1111	16	-0.3477856686442138
1111	17	-0.43603522882916934
1111	18	-0.40836792190621907
1111	19	-0.4367284391148516
1111	20	-0.390353941259896
1111	21	-0.4029163188140714
1111	22	-0.40698648328707776
1111	23	-0.3833793267790213
1111	24	-0.3590277582915178
1111	25	-0.3798042175531222
1112	1	-0.4271116001918216
1112	2	-0.3710671331248392
1112	3	-0.4222008530450232
1112	4	-0.31129598229081523
1112	5	-0.3377876669608071
1112	6	-1.2317790255122532
1112	7	-0.5660585768329724
1112	8	-0.47037314065857316
1112	9	-0.5181830866168085
1112	10	-0.5169006896281587
1112	11	-0.5547919301921027
1112	12	-0.5348357121984577
1112	13	-0.4539230349054293
1112	14	-0.5811466276662784
1112	15	-0.5274198527479257
1112	16	-0.5401902915333068
1112	17	-0.603122600807346
1112	18	-0.6174598493597259
1112	19	-0.6530945947790983
1112	20	-0.6440215833069161
1112	21	-0.6237140719575649
1112	22	-0.7973094285421496
1112	23	-0.7067445302742499
1112	24	-0.7163889785210955
1112	25	-0.6574202729293006
1113	1	-0.5475895498509011
1113	2	-0.6676956257638764
1113	3	-0.5304261019083256
1113	4	-0.4573540303435877
1113	5	-0.4111203790751503
1113	6	-1.0077052144092775
1113	7	-0.7580728267769388
1113	8	-0.5677726440209128
1113	9	-0.6510548976357242
1113	10	-0.6700884109976997
1113	11	-0.644579029683662
1113	12	-0.6977179256218164
1113	13	-0.6998819169587662
1113	14	-0.8193510411821094
1113	15	-0.8319471689846338
1113	16	-0.8755627380359456
1113	17	-0.8013710003246146
1113	18	-0.765348906237584
1113	19	-0.7781404327846317
1113	20	-0.7721272463087132
1113	21	-0.790660086259102
1113	22	-0.898626870884712
1113	23	-0.7780874530429003
1113	24	-0.9167264177664052
1113	25	-0.9108663707699236
1114	1	-0.8546181749359789
1114	2	-0.9127345209068913
1114	3	-0.9351349208353952
1114	4	-0.8193011146823181
1114	5	-1.0790326761313693
1114	6	-1.6450010153795844
1114	7	-1.3089791534685276
1114	8	-1.3982750085139983
1114	9	-1.5809983105286207
1114	10	-1.6477552832713158
1114	11	-1.3470175222237728
1114	12	-1.399446359046678
1114	13	-1.4416757330348275
1114	14	-1.7174534020964316
1114	15	-1.5582723175771989
1114	16	-1.5308091897291192
1114	17	-1.5187723525852093
1114	18	-1.7696760949234402
1114	19	-1.5966374757126687
1114	20	-1.5532519455879736
1114	21	-1.5597799004078325
1114	22	-1.5367936364401658
1114	23	-1.5864270778111873
1114	24	-1.5482897704317367
1114	25	-1.619919266414719
1115	1	-1.2540213480751738
1115	2	-1.8698878443383435
1115	3	-1.4478998571115795
1115	4	-1.1839951944288316
1115	5	-1.228991028516127
1115	6	-1.759689511621751
1115	7	-1.3408779801687132
1115	8	-1.3914021619449812
1115	9	-1.428833393410244
1115	10	-1.5571673427762889
1115	11	-1.5759912158269884
1115	12	-1.4779639994527187
1115	13	-1.4916086857517428
1115	14	-1.7150127623866496
1115	15	-1.6635511364755544
1115	16	-1.7357206029849337
1115	17	-1.7103329284812219
1115	18	-1.8828537783149244
1115	19	-1.9185278985100922
1115	20	-1.8395200529026283
1115	21	-1.9099062705399916
1115	22	-2.0039734159324496
1115	23	-1.832518659947148
1115	24	-2.0032108681275815
1115	25	-1.9574178705386345
1116	1	-1.00110580161666
1116	2	-1.0340179562180225
1116	3	-1.0606558263453039
1116	4	-0.9949336151353023
1116	5	-1.0256954212705764
1116	6	-1.2397400116842334
1116	7	-1.265163147696832
1116	8	-1.2730561397098512
1116	9	-1.4041190281548808
1116	10	-1.4705183725789936
1116	11	-1.475060928873127
1116	12	-1.3883216948647075
1116	13	-1.4187510514553523
1116	14	-1.5847229532022524
1116	15	-1.5801379486879554
1116	16	-1.621438679961642
1116	17	-1.5851110593061435
1116	18	-1.5561845548976194
1116	19	-1.5532418119989586
1116	20	-1.6175302698659308
1116	21	-1.7622708183305278
1116	22	-1.63954693740925
1116	23	-1.6982787101382115
1116	24	-1.7485251206173373
1116	25	-1.899571889275407
1201	1	0.7132648436744446
1201	2	0.8940898334446743
1201	3	0.8629132687329957
1201	4	0.6858996435088827
1201	5	0.8381343676026844
1201	6	0.8782183063795514
1201	7	0.8378825772120422
1201	8	0.8651799089783978
1201	9	1.0121415136080785
1201	10	0.9534294927709865
1201	11	1.0008510384338294
1201	12	1.047782284752735
1201	13	1.0764172585009177
1201	14	1.2709150072370647
1201	15	1.2064874981307625
1201	16	1.2651468070941974
1201	17	1.2244352407305499
1201	18	1.2917034714778808
1201	19	1.2559047363224352
1201	20	1.165399592275456
1201	21	1.2245874018112985
1201	22	1.200793701592552
1201	23	1.1561990560802258
1201	24	1.2259081556954357
1201	25	1.2406897547195754
1202	1	0.5514405596747736
1202	2	0.7090522669960109
1202	3	0.6857619823425409
1202	4	0.6158861606421056
1202	5	0.6850663914402446
1202	6	0.7766459504502095
1202	7	0.7373497314646329
1202	8	0.7626262122432337
1202	9	0.9059630463626505
1202	10	0.9667224007083561
1202	11	0.9862840411842271
1202	12	0.9347395934224352
1202	13	0.9746743674829261
1202	14	1.1113115165990948
1202	15	1.0935819955217099
1202	16	1.1038621433839921
1202	17	1.1019740003645353
1202	18	1.104692900305885
1202	19	1.0825306221702107
1202	20	1.0258965923758012
1202	21	1.116544152727343
1202	22	1.175783892089619
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2211	4	-0.12062945274044523
2211	5	-0.11788108646091189
2211	6	-0.11286671860267461
2211	7	-0.18597760604956903
2211	8	-0.1082129094371922
2211	9	-0.18662775477732652
2211	10	-0.21104564320707908
2211	11	-0.23222673680399453
2211	12	-0.23610259464287964
2211	13	-0.10610408615445266
2211	14	-0.2521342619410234
2211	15	-0.2259777861887855
2211	16	-0.22500231591114073
2211	17	-0.26202866531696145
2211	18	-0.24739197679128466
2211	19	-0.19630246669804308
2211	20	-0.252943344024537
2211	21	-0.2888363760887316
2211	22	-0.2611107253736833
2211	23	-0.40047993911536395
2211	24	-0.32249263844380494
2211	25	-0.3981513914735544
2212	1	-0.3513438425137423
2212	2	-0.4425067457397951
2212	3	-0.4216184931972009
2212	4	-0.44654723243332484
2212	5	-0.33151328277252645
2212	6	-0.29072244085399745
2212	7	-0.43688501883491426
2212	8	-0.4250584388253955
2212	9	-0.4678042233621582
2212	10	-0.48651829319386053
2212	11	-0.4356435394073799
2212	12	-0.5654478615017311
2212	13	-0.500203631433962
2212	14	-0.6624959559982884
2212	15	-0.6191160483147726
2212	16	-0.729119649437294
2212	17	-0.5966986169080712
2212	18	-0.5368435888874288
2212	19	-0.5835619707081605
2212	20	-0.5694102853460308
2212	21	-0.5790186384835181
2212	22	-0.5825808539434192
2212	23	-0.6746001863352689
2212	24	-0.672849335601434
2212	25	-0.7032303225334999
2213	1	-0.6107534094925171
2213	2	-0.9162486030521677
2213	3	-0.6813273889505496
2213	4	-0.6392446818509612
2213	5	-0.6663810583836423
2213	6	-0.5436472822694398
2213	7	-0.7541230868571986
2213	8	-0.7172993375094734
2213	9	-0.8507893051417028
2213	10	-0.7568353787816022
2213	11	-0.8217429186698268
2213	12	-0.81673115109691
2213	13	-0.8571928602385128
2213	14	-0.9390883150507392
2213	15	-1.045284963986532
2213	16	-1.0919215220107716
2213	17	-1.057528269115501
2213	18	-1.1476435803152896
2213	19	-0.982750271055636
2213	20	-0.9663294158274738
2213	21	-0.9834488941829775
2213	22	-0.9847641083725804
2213	23	-1.015685651903624
2213	24	-0.9778231078957376
2213	25	-1.0976750918079858
2214	1	-0.8672476499261137
2214	2	-0.9357786119089653
2214	3	-0.9345070737200345
2214	4	-0.8561458749370985
2214	5	-1.0224716211278988
2214	6	-0.9870503661958736
2214	7	-0.9596058038553679
2214	8	-1.0728881887999933
2214	9	-1.1380014261131208
2214	10	-1.106354915699491
2214	11	-1.1339511259030104
2214	12	-1.218769207526229
2214	13	-1.0777843857789193
2214	14	-1.2809987753394552
2214	15	-1.483433006223791
2214	16	-1.3912901441895684
2214	17	-1.3264598814631228
2214	18	-1.4271017832458455
2214	19	-1.3668521947283025
2214	20	-1.4274373247891248
2214	21	-1.5192799951134006
2214	22	-1.4104609192634001
2214	23	-1.5045883423049204
2214	24	-1.625280601223352
2214	25	-1.6439970797685675
2215	1	-1.4966356428688528
2215	2	-3.2932300123006755
2215	3	-1.8743072258720161
2215	4	-1.5850407228120957
2215	5	-1.5060834501349731
2215	6	-1.422964168322764
2215	7	-1.566556775307987
2215	8	-1.556986148776474
2215	9	-1.8463347144842857
2215	10	-1.8604109529497492
2215	11	-1.8509495234513125
2215	12	-1.783025901943546
2215	13	-1.7283954216533317
2215	14	-2.084822538025527
2215	15	-2.1352758256755067
2215	16	-2.1925812778297242
2215	17	-2.1803114307695353
2215	18	-2.0711600326348076
2215	19	-2.009199004122884
2215	20	-2.0237867563773833
2215	21	-2.0730462961477087
2215	22	-2.103159242893895
2215	23	-2.2530843577575155
2215	24	-2.282026814959117
2215	25	-2.353925805738534
2216	1	-1.1991567221046537
2216	2	-1.9204058293268496
2216	3	-1.393691430339942
2216	4	-1.2190156305533932
2216	5	-1.307859874580302
2216	6	-1.2383801292751428
2216	7	-1.3350141782618437
2216	8	-1.4448577847253574
2216	9	-1.6328811441187554
2216	10	-1.5227691405964308
2216	11	-1.6656845743812738
2216	12	-1.6867038696971193
2216	13	-1.8530491058111807
2216	14	-1.8000638593860856
2216	15	-1.8934992958669241
2216	16	-1.8404272578265122
2216	17	-1.8823288736560642
2216	18	-2.1441844057893036
2216	19	-1.9939113229310124
2216	20	-2.0623141468720405
2216	21	-2.0765735944495205
2216	22	-2.0260725895205596
2216	23	-2.273037523791565
2216	24	-2.191301151894997
2216	25	-2.243897331319417
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57662.0
3	1.0
4	986.0
5	8304.0
6	10364.0
7	8322.0
8	6960.0
9	5927.0
10	5454.0
11	5038.0
12	4676.0
13	4523.0
14	4514.0
15	4692.0
16	4755.0
17	5076.0
18	5660.0
19	7003.0
20	9330.0
21	12237.0
22	15406.0
23	18588.0
24	22138.0
25	25405.0
26	29028.0
27	33094.0
28	38533.0
29	47116.0
30	60578.0
31	78617.0
32	103179.0
33	135917.0
34	183273.0
35	252112.0
36	365001.0
37	630215.0
38	1830020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.29139890299163	18.624902363274433	13.15433991364505	25.929358820088886
2	16.33421484313301	21.429674877861956	36.91700653730044	25.31910374170459
3	17.866457542433803	25.263756948528904	29.515949683442155	27.35383582559514
4	12.949389361200723	17.433406011925626	36.93129496616584	32.685909660707814
5	15.099174128862844	36.08247269488783	33.427188142445004	15.391165033804324
6	31.887395963581284	37.566786176056816	16.640108565983862	13.90570929437804
7	28.304203040337146	31.456359517094967	21.96198984441839	18.2774475981495
8	26.50767756931844	33.01080033368897	20.246157502184573	20.23536459480802
9	27.066321888736084	15.048244566219768	19.364075344458158	38.52135820058599
10	15.946239295840344	26.88270409825594	31.559556572727875	25.611500033175837
11	35.26285978253497	22.526559282516896	23.225960736277727	18.984620198670417
12	24.54222311566547	24.484787478238825	28.86160586081166	22.11138354528404
13	29.000724193638238	20.737761436998248	25.214392265364356	25.047122103999158
14	23.46044380609062	20.723089190225565	26.002059136682558	29.814407867001258
15	24.42457310471169	27.547747873106122	23.26665050703526	24.761028515146933
16	24.740332419065783	26.751176379861953	24.153918786403285	24.354572414668983
17	24.143612899712092	25.934565673219506	25.439178902182714	24.48264252488569
18	25.083558309119454	24.887850416392926	26.4606428110644	23.567948463423217
19	26.08998636720227	25.06805877822703	25.81827111765424	23.02368373691646
20	25.169754289809543	25.000278498780176	25.691623870687447	24.138343340722834
21	25.57541730720197	24.94589797479447	25.62002579469699	23.85865892330657
22	24.630146169125265	24.865757491142915	25.990864677213978	24.51323166251785
23	23.891088380963417	24.33665571747877	26.333749961939006	25.438505939618807
24	24.20000712953171	25.5639261538949	25.95087653631506	24.285190180258326
25	24.3071029115033	24.949939313253278	26.130774926310718	24.612182848932704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1091.0
1	1091.0
2	1823.0
3	2555.0
4	2555.0
5	2555.0
6	6014.0
7	9473.0
8	9473.0
9	9473.0
10	11302.5
11	13132.0
12	13132.0
13	13132.0
14	16449.0
15	19766.0
16	19766.0
17	19766.0
18	33972.5
19	48179.0
20	48179.0
21	48179.0
22	69624.0
23	91069.0
24	91069.0
25	91069.0
26	124964.0
27	158859.0
28	158859.0
29	158859.0
30	188554.5
31	218250.0
32	218250.0
33	218250.0
34	256613.5
35	294977.0
36	294977.0
37	294977.0
38	322636.0
39	350295.0
40	350295.0
41	350295.0
42	386053.5
43	421812.0
44	421812.0
45	421812.0
46	449374.0
47	476936.0
48	476936.0
49	476936.0
50	481572.0
51	486208.0
52	486208.0
53	486208.0
54	456299.0
55	426390.0
56	426390.0
57	426390.0
58	389898.5
59	353407.0
60	353407.0
61	353407.0
62	308933.5
63	264460.0
64	264460.0
65	264460.0
66	218418.5
67	172377.0
68	172377.0
69	172377.0
70	134312.0
71	96247.0
72	96247.0
73	96247.0
74	77202.0
75	58157.0
76	58157.0
77	58157.0
78	49287.0
79	40417.0
80	40417.0
81	40417.0
82	30571.5
83	20726.0
84	20726.0
85	20726.0
86	15400.5
87	10075.0
88	10075.0
89	10075.0
90	7225.5
91	4376.0
92	4376.0
93	4376.0
94	2396.0
95	416.0
96	416.0
97	416.0
98	235.0
99	54.0
100	54.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32920728845479763
2	0.010446309927658066
3	0.0
4	0.0
5	0.0472064289858861
6	0.003292320427437258
7	0.0037626519170711518
8	3.4656004499339555E-4
9	0.009629418393030777
10	0.015347659135421802
11	0.006188572232024921
12	0.009901715571239873
13	0.01804587662858467
14	0.003638880472430653
15	0.020620322677107037
16	0.010025487015880371
17	0.005792503609175326
18	2.4754288928099683E-4
19	0.004480526295986042
20	0.00467856060741084
21	0.0028219889378033637
22	0.0
23	0.003341829005293457
24	0.004158720539920747
25	0.002648708915306666
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4039704.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.389503295121024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.69012745384012	45.74953342056138
2	8.21428321160853	8.771130011864619
3	2.2892914064632364	3.666723932665836
4	1.0564661164351885	2.256168048184008
5	0.5982579223325669	1.597034665785342
6	0.3891568634955853	1.2466134987549908
7	0.2654938799402024	0.9922210464531331
8	0.20624649486241212	0.880911833365113
9	0.1580957984097197	0.7596590535126467
>10	0.942435343149058	9.572962584055803
>50	0.09336876543121854	3.486881427677682
>100	0.07678750364166355	8.46385879956463
>500	0.012580640822513632	4.718759770599788
>1k	0.0071756247389397	6.6963675054311125
>5k	1.3978489751181232E-4	0.449474398594735
>10k+	9.318993167454154E-5	0.6917000029293007
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	16473	0.40777740151258607	No Hit
TATCAACGCAGAGTACTTTTTTTTT	11332	0.2805156021332256	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6333	0.15676891178165528	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6207	0.15364987137671474	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5528	0.13684170919453503	No Hit
CTATTGGAGCTGGAATTACCGCGGC	4336	0.10733459679224022	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4234	0.10480965932157404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.4754288928099684E-5	0.0	0.0	0.0	0.0
3	2.4754288928099684E-5	0.0	0.0	2.4754288928099684E-5	0.0
4	2.4754288928099684E-5	0.0	0.0	4.950857785619937E-5	0.0
5	2.4754288928099684E-5	0.0	0.0	4.950857785619937E-5	0.0
6	2.4754288928099684E-5	0.0	0.0	4.950857785619937E-5	2.4754288928099684E-5
7	2.4754288928099684E-5	0.0	0.0	4.950857785619937E-5	2.4754288928099684E-5
8	2.4754288928099684E-5	0.0	0.0	7.426286678429904E-5	2.4754288928099684E-5
9	2.4754288928099684E-5	0.0	0.0	7.426286678429904E-5	2.4754288928099684E-5
10	2.4754288928099684E-5	0.0	0.0	7.426286678429904E-5	7.426286678429904E-5
11	2.4754288928099684E-5	0.0	0.0	9.901715571239874E-5	7.426286678429904E-5
12	4.950857785619937E-5	0.0	0.0	9.901715571239874E-5	1.7328002249669778E-4
13	4.950857785619937E-5	0.0	0.0	1.4852573356859808E-4	1.9803431142479747E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2290	0.0	16.230476	1
GTATCAA	7190	0.0	13.108998	1
ATTAACG	55	0.0030685132	12.091642	3
CGTCGTA	535	0.0	11.895559	10
CGAACGA	310	0.0	11.028556	16
CCGTCGT	580	0.0	10.972629	9
CGAACGT	330	0.0	10.941412	4
CGAGCCG	750	0.0	10.891431	15
TCGAACG	350	0.0	10.857801	3
AACGTCT	350	0.0	10.582817	6
CCGTCAA	425	0.0	10.500537	18
ACCGTCG	625	0.0	10.485648	8
ATACCGT	600	0.0	10.288851	6
AGAACCG	500	0.0	10.261893	5
TACCGTC	600	0.0	10.131062	7
AACCGCG	445	0.0	10.031459	7
GAACGTC	380	0.0	10.001845	5
GTCGTAG	605	0.0	9.891079	11
GGTAGCG	125	2.5777335E-6	9.878519	11
GCTCGTA	385	0.0	9.868772	9
>>END_MODULE