FastQCFastQC Report
Thu 2 Feb 2017
SRR4050266_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050266_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3998439
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN133750.3345055407872922No Hit
GTATCAACGCAGAGTACTTTTTTTT105040.2627025196582966No Hit
TATCAACGCAGAGTACTTTTTTTTT61710.1543352293232434No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN58890.14728247698664404No Hit
CTATTGGAGCTGGAATTACCGCGGC40640.1016396648792191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA250.00603524318.99915515
GGTATCA20850.014.9988131
GTATCAA68300.013.17941951
CGCCAGT5300.010.93375218
CGAACGA2700.010.90678616
ATCGCCA5550.010.78316916
GTTTTCG6000.010.76618915
TATCACG803.771492E-410.6881032
CGAGCCG6850.010.40099815
GTATTAG6500.010.3827411
ATCACGC1201.5258483E-610.2914693
AACCGCG5000.010.2595447
GTCTAAA3250.010.2365051
CGTCGTA4750.010.19954710
CATCGCC6000.010.13288315
ACCGTCG5000.010.0694278
CGGTTCT6850.09.98520912
TCGCCAG6000.09.97455717
GCTCGTA3650.09.8899719
ATACCGT5700.09.8327736
Produced by FastQC (version 0.11.5)