##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050266_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3998439 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.935084166596013 31.0 30.0 34.0 26.0 34.0 2 29.911290881266414 31.0 30.0 34.0 26.0 34.0 3 30.085821241739588 31.0 31.0 34.0 26.0 34.0 4 33.54721780174713 37.0 35.0 37.0 28.0 37.0 5 33.608950642988425 37.0 35.0 37.0 28.0 37.0 6 33.661032968115805 37.0 35.0 37.0 29.0 37.0 7 33.599399165524346 37.0 35.0 37.0 28.0 37.0 8 33.54864385826569 37.0 35.0 37.0 28.0 37.0 9 35.03086529518144 39.0 35.0 39.0 28.0 39.0 10 34.90611586171504 39.0 35.0 39.0 27.0 39.0 11 34.93362909875579 39.0 35.0 39.0 27.0 39.0 12 34.85265524871081 39.0 35.0 39.0 27.0 39.0 13 34.84449581449161 39.0 35.0 39.0 27.0 39.0 14 35.899937200492495 40.0 36.0 41.0 27.0 41.0 15 35.877430667317924 40.0 36.0 41.0 27.0 41.0 16 35.88108284257932 40.0 36.0 41.0 27.0 41.0 17 35.85128046220037 40.0 36.0 41.0 27.0 41.0 18 35.78504886531969 40.0 36.0 41.0 27.0 41.0 19 35.819529321317646 40.0 36.0 41.0 27.0 41.0 20 35.78647567213105 40.0 36.0 41.0 27.0 41.0 21 35.704221822566254 40.0 36.0 41.0 27.0 41.0 22 35.61222342018973 40.0 36.0 41.0 27.0 41.0 23 35.53238576354423 40.0 36.0 41.0 26.0 41.0 24 35.47486356550644 40.0 36.0 41.0 26.0 41.0 25 35.137001214724044 39.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3647286535149803 1101 2 1.424821168257001 1101 3 1.4428835678428413 1101 4 1.5372560610939914 1101 5 1.4848920117765445 1101 6 1.5200578992834295 1101 7 1.5115226095846381 1101 8 1.5661790135001326 1101 9 1.6840005076979523 1101 10 1.7432263619119084 1101 11 1.6790236461996955 1101 12 1.7113393159444783 1101 13 1.683088791446039 1101 14 1.9892072615301544 1101 15 1.9229866355158975 1101 16 1.9319934509700687 1101 17 1.950798368869954 1101 18 1.9170126488356303 1101 19 1.9195609448730977 1101 20 1.8851233380596213 1101 21 1.9116084001398619 1101 22 1.9472464524287219 1101 23 1.9019648239385916 1101 24 1.9618914731783335 1101 25 1.928168217634493 1102 1 1.1574200594548998 1102 2 1.12283928115858 1102 3 1.2135856164236358 1102 4 1.316587465673102 1102 5 1.3146091199493242 1102 6 1.1688365045701659 1102 7 1.1536149771611548 1102 8 1.2025481265944933 1102 9 1.382841554158155 1102 10 1.4508545328745655 1102 11 1.3929358373249698 1102 12 1.391446025854549 1102 13 1.364214546977152 1102 14 1.5419110848294295 1102 15 1.5571438001621445 1102 16 1.5213628589058388 1102 17 1.5596672126380255 1102 18 1.6464612103497558 1102 19 1.6259338427105448 1102 20 1.5861115529082923 1102 21 1.5951001162302632 1102 22 1.702751410867421 1102 23 1.6496013440161548 1102 24 1.599394449942558 1102 25 1.5905658027699516 1103 1 0.8969290511764605 1103 2 0.9152042070631445 1103 3 0.9712966837038444 1103 4 0.9455649449653336 1103 5 0.9913780316889245 1103 6 1.0050217566606037 1103 7 1.0269858055751797 1103 8 0.9313021082808177 1103 9 1.0228942445434868 1103 10 1.0706001648076366 1103 11 1.1081900571043874 1103 12 1.1139233137059392 1103 13 1.0524690096815092 1103 14 1.2876905723011305 1103 15 1.2154179267903515 1103 16 1.334534522195213 1103 17 1.313363187248541 1103 18 1.2096811420575264 1103 19 1.3023749224169165 1103 20 1.2661267282191204 1103 21 1.382093468617711 1103 22 1.422176975034759 1103 23 1.345162682229315 1103 24 1.303573292013695 1103 25 1.3720154348336422 1104 1 0.8600745083243382 1104 2 0.9053425725438515 1104 3 0.9087770749094979 1104 4 1.0391327160646213 1104 5 1.0121654510643197 1104 6 0.9903600301773068 1104 7 0.9207661313621429 1104 8 0.9249925834420551 1104 9 1.144541649041976 1104 10 1.2044586407860365 1104 11 1.1931981631934505 1104 12 1.1458417078197272 1104 13 1.119550859246715 1104 14 1.2326535097185385 1104 15 1.2304752073647762 1104 16 1.2723811363540207 1104 17 1.2628233310031334 1104 18 1.282879526539432 1104 19 1.2984897634283783 1104 20 1.2705286083578713 1104 21 1.3847948792341072 1104 22 1.4037961178930942 1104 23 1.4000782255230035 1104 24 1.3900423193246354 1104 25 1.3588473549248619 1105 1 0.7310208148301491 1105 2 0.8917925583188833 1105 3 0.9044700616149193 1105 4 0.9358352911686154 1105 5 0.8813807709723562 1105 6 0.863928805984095 1105 7 0.8493467869410267 1105 8 0.830774995228019 1105 9 1.0482099996374998 1105 10 1.0788661515701037 1105 11 0.983155488283991 1105 12 1.0156887701712307 1105 13 1.065472598845183 1105 14 1.1658238325869092 1105 15 1.2280901797222015 1105 16 1.2322916343817454 1105 17 1.2301754105399425 1105 18 1.206750717515419 1105 19 1.2974677185697487 1105 20 1.2614856797057072 1105 21 1.332938174659823 1105 22 1.3597028636044541 1105 23 1.338926387426227 1105 24 1.2737878692158304 1105 25 1.2259181645178785 1106 1 0.6063142861642277 1106 2 0.7308278715673957 1106 3 0.692884151754896 1106 4 0.7820734523306356 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0.8522893392140247 1107 18 0.806513584038143 1107 19 0.8877989317067545 1107 20 0.93067920543443 1107 21 0.9079663683000945 1107 22 0.8615806962062038 1107 23 0.9003031102208254 1107 24 0.873784497649801 1107 25 0.8568986202412177 1108 1 0.41430662437512566 1108 2 0.5314877286742004 1108 3 0.48655200575065294 1108 4 0.583927671485938 1108 5 0.5363402857851725 1108 6 0.4583072392867322 1108 7 0.4409745560749059 1108 8 0.4637118383360104 1108 9 0.45466815435971597 1108 10 0.47821881194965954 1108 11 0.42882955342015805 1108 12 0.3989066281079161 1108 13 0.457956854605726 1108 14 0.45260078831252315 1108 15 0.46444577197021175 1108 16 0.46967645005506853 1108 17 0.557072181250895 1108 18 0.45692313678318897 1108 19 0.4882144453555952 1108 20 0.5424420827159011 1108 21 0.5864882757404857 1108 22 0.5746445966880032 1108 23 0.49692594288299574 1108 24 0.48176974878695233 1108 25 0.5719307175719948 1109 1 0.29442772058999367 1109 2 0.5020084579465482 1109 3 0.32132991612183304 1109 4 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2216 22 -3.281579290418165 2216 23 -3.1692226444251617 2216 24 -3.2217196238960213 2216 25 -3.542130459574306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 147736.0 3 10.0 4 1005.0 5 6211.0 6 8374.0 7 7859.0 8 7301.0 9 6673.0 10 6128.0 11 6086.0 12 5857.0 13 5551.0 14 5477.0 15 5629.0 16 5734.0 17 6109.0 18 6955.0 19 8474.0 20 11118.0 21 14121.0 22 17590.0 23 21166.0 24 24557.0 25 27880.0 26 31217.0 27 35307.0 28 40527.0 29 48068.0 30 59875.0 31 77019.0 32 98445.0 33 129614.0 34 175438.0 35 242592.0 36 355679.0 37 628552.0 38 1712505.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88720928480673 17.967843672942582 12.634227429552885 25.51071961269781 2 16.25552121893303 20.86734291708734 37.98729581343084 24.88984005054879 3 18.107782711745728 25.03268089093524 29.233340301898387 27.626196095420646 4 12.661441949414687 16.852962632322445 37.74063893630185 32.744956481961026 5 15.357762318095764 35.65210928928709 33.468212970765585 15.52191542185156 6 32.01915143667266 37.067930664846074 16.92864061832789 13.984277280153373 7 28.52708193595237 31.158878372800995 22.076763018131224 18.237276673115407 8 26.428142586376612 32.82929708787176 20.463730477917363 20.278829847834263 9 27.094933295311613 15.18255589079269 19.381176377529467 38.34133443636623 10 16.026743061018504 26.63562831256091 31.540731101604642 25.796897524815947 11 35.213638910386635 22.54436933668698 23.301182443326564 18.940809309599818 12 24.632041077671637 24.30772378032082 28.874316906849916 22.18591823515763 13 28.932802659616545 20.75877977292687 25.171705598023227 25.136711969433357 14 23.558939867736687 20.83733003166368 25.788448741855742 29.815281358743896 15 24.479792473799087 27.466811252390688 23.091421832125192 24.96197444168503 16 24.796883868892124 26.809767310502835 24.0345795623943 24.358769258210742 17 24.210251027576906 25.933083164065117 25.311976731940117 24.544689076417857 18 25.260437794509 24.992479013044942 26.15421323925507 23.59286995319099 19 26.213516153716874 25.2176678105119 25.403846071472397 23.164969964298827 20 25.264505140950995 25.176450136176626 25.274557772397337 24.284486950475042 21 25.742820711984223 25.11901129734974 25.21210401896018 23.92606397170586 22 24.77564428018582 25.034109729140404 25.59536122801576 24.59488476265802 23 23.964353854405772 24.578779246489944 25.895354699152506 25.561512199951782 24 24.296604118513457 25.716648249402507 25.566337171481106 24.420410460602927 25 24.444998180900157 25.151675637869126 25.645982006532638 24.757344174698083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18731.0 1 18731.0 2 15277.0 3 11823.0 4 11823.0 5 11823.0 6 9560.5 7 7298.0 8 7298.0 9 7298.0 10 9546.5 11 11795.0 12 11795.0 13 11795.0 14 15927.0 15 20059.0 16 20059.0 17 20059.0 18 33282.5 19 46506.0 20 46506.0 21 46506.0 22 66511.0 23 86516.0 24 86516.0 25 86516.0 26 118404.0 27 150292.0 28 150292.0 29 150292.0 30 179593.5 31 208895.0 32 208895.0 33 208895.0 34 248419.0 35 287943.0 36 287943.0 37 287943.0 38 317182.5 39 346422.0 40 346422.0 41 346422.0 42 380954.0 43 415486.0 44 415486.0 45 415486.0 46 442183.5 47 468881.0 48 468881.0 49 468881.0 50 475617.0 51 482353.0 52 482353.0 53 482353.0 54 452400.0 55 422447.0 56 422447.0 57 422447.0 58 386242.0 59 350037.0 60 350037.0 61 350037.0 62 306630.0 63 263223.0 64 263223.0 65 263223.0 66 217245.0 67 171267.0 68 171267.0 69 171267.0 70 133485.5 71 95704.0 72 95704.0 73 95704.0 74 76672.5 75 57641.0 76 57641.0 77 57641.0 78 48571.0 79 39501.0 80 39501.0 81 39501.0 82 29815.0 83 20129.0 84 20129.0 85 20129.0 86 15311.0 87 10493.0 88 10493.0 89 10493.0 90 7486.5 91 4480.0 92 4480.0 93 4480.0 94 2456.5 95 433.0 96 433.0 97 433.0 98 258.5 99 84.0 100 84.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40063134638292597 2 0.6908195923459131 3 0.7060005167016428 4 0.7150540498429513 5 0.7246327879454957 6 0.7258832759484388 7 0.7429149225485245 8 0.7322857745235078 9 0.7197308749739586 10 0.713078278798301 11 0.726058344268851 12 0.7297347789975037 13 0.7289094569155613 14 0.7387382926186945 15 0.7352369262104536 16 0.7292345837963266 17 0.7351118774101593 18 0.7389133609391064 19 0.7418395028659934 20 0.7335112527663921 21 0.7322357550033901 22 0.7410391905441098 23 0.7303350132389165 24 0.733711330846863 25 0.7363623654131024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3998439.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.891461445356384 #Duplication Level Percentage of deduplicated Percentage of total 1 85.83221538284882 46.256235260743225 2 8.178683965030638 8.81522463150406 3 2.2460844516777394 3.63134320891816 4 1.031984487729047 2.224606089306232 5 0.579598379476574 1.5617701860676414 6 0.3895662048012443 1.2596575263876042 7 0.2791753281657217 1.0531616504036394 8 0.20036789066925548 0.8638494763911653 9 0.1476130357125976 0.7159574000643721 >10 0.9264982548226078 9.451993964394594 >50 0.0933544620808581 3.4895231060385488 >100 0.0751981951662822 8.286635057347919 >500 0.012123643136477277 4.513865753313202 >1k 0.007349080950882507 6.969569145007348 >5k 9.361886561633768E-5 0.304229026461328 >10k+ 9.361886561633768E-5 0.6023785176509163 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 13375 0.3345055407872922 No Hit GTATCAACGCAGAGTACTTTTTTTT 10504 0.2627025196582966 No Hit TATCAACGCAGAGTACTTTTTTTTT 6171 0.1543352293232434 No Hit GNNNNNNNNNNNNNNNNNNNNNNNN 5889 0.14728247698664404 No Hit CTATTGGAGCTGGAATTACCGCGGC 4064 0.1016396648792191 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5009760058862972E-5 2 0.0 0.0 0.0 0.0 2.5009760058862972E-5 3 0.0 0.0 0.0 0.0 2.5009760058862972E-5 4 0.0 0.0 0.0 0.0 2.5009760058862972E-5 5 0.0 2.5009760058862972E-5 0.0 0.0 2.5009760058862972E-5 6 0.0 2.5009760058862972E-5 0.0 0.0 5.0019520117725944E-5 7 0.0 2.5009760058862972E-5 0.0 0.0 5.0019520117725944E-5 8 0.0 2.5009760058862972E-5 0.0 0.0 5.0019520117725944E-5 9 0.0 2.5009760058862972E-5 0.0 0.0 5.0019520117725944E-5 10 0.0 2.5009760058862972E-5 0.0 0.0 5.0019520117725944E-5 11 0.0 2.5009760058862972E-5 0.0 2.5009760058862972E-5 5.0019520117725944E-5 12 0.0 2.5009760058862972E-5 0.0 2.5009760058862972E-5 1.5005856035317782E-4 13 0.0 2.5009760058862972E-5 0.0 5.0019520117725944E-5 1.5005856035317782E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACTA 25 0.006035243 18.999155 15 GGTATCA 2085 0.0 14.998813 1 GTATCAA 6830 0.0 13.1794195 1 CGCCAGT 530 0.0 10.933752 18 CGAACGA 270 0.0 10.906786 16 ATCGCCA 555 0.0 10.783169 16 GTTTTCG 600 0.0 10.766189 15 TATCACG 80 3.771492E-4 10.688103 2 CGAGCCG 685 0.0 10.400998 15 GTATTAG 650 0.0 10.382741 1 ATCACGC 120 1.5258483E-6 10.291469 3 AACCGCG 500 0.0 10.259544 7 GTCTAAA 325 0.0 10.236505 1 CGTCGTA 475 0.0 10.199547 10 CATCGCC 600 0.0 10.132883 15 ACCGTCG 500 0.0 10.069427 8 CGGTTCT 685 0.0 9.985209 12 TCGCCAG 600 0.0 9.974557 17 GCTCGTA 365 0.0 9.889971 9 ATACCGT 570 0.0 9.832773 6 >>END_MODULE