FastQCFastQC Report
Thu 2 Feb 2017
SRR4050266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3998439
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT163100.40790918656005504No Hit
TATCAACGCAGAGTACTTTTTTTTT114340.2859615965130392No Hit
GGTATCAACGCAGAGTACTTTTTTT63780.15951224965542804No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63250.1581867323723083No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54720.13685340704209817No Hit
GAGTACTTTTTTTTTTTTTTTTTTT42020.1050910117673422No Hit
CTATTGGAGCTGGAATTACCGCGGC41520.10384052376439905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.015.9268221
GTATCAA70950.012.7602351
TACCGAA550.003085651212.0826487
ATACCGT6100.011.5213266
CGTCGTA5950.011.32881410
CGCGCTA2350.011.31141516
TACCGTC6100.011.0498227
GCGCGCT2450.010.84972515
CGAGCCG7650.010.7965415
TAGATCG1158.6930595E-710.7519655
AGGCCCG8200.010.53587510
ACCGTCG6750.010.408098
CCGTCGT6650.010.2790729
ATCGCCA4350.010.25735416
ACGCATC2600.010.22416210
CGAACGA2700.010.19673216
GCTCGTA4250.09.8289439
CGACCAT6150.09.72542310
CGGTTCT7550.09.682363512
GCCGCCT8800.09.6012718
Produced by FastQC (version 0.11.5)