##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3998439
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.7921909024997	31.0	31.0	34.0	27.0	34.0
2	30.797277387500472	31.0	31.0	34.0	27.0	34.0
3	31.006192916785775	31.0	31.0	34.0	27.0	34.0
4	34.61198857854278	37.0	35.0	37.0	31.0	37.0
5	34.52803031382997	37.0	35.0	37.0	32.0	37.0
6	34.55967966498926	37.0	35.0	37.0	31.0	37.0
7	34.453681049029385	37.0	35.0	37.0	31.0	37.0
8	34.4225433975609	37.0	35.0	37.0	31.0	37.0
9	36.03601105331356	39.0	37.0	39.0	31.0	39.0
10	35.92995116344153	39.0	37.0	39.0	31.0	39.0
11	35.92435297874996	39.0	37.0	39.0	31.0	39.0
12	35.8332756858364	39.0	37.0	39.0	30.0	39.0
13	35.83104556553195	39.0	37.0	39.0	30.0	39.0
14	36.99822455713342	40.0	37.0	41.0	31.0	41.0
15	36.96901665875108	40.0	37.0	41.0	31.0	41.0
16	36.93833093364685	40.0	37.0	41.0	31.0	41.0
17	36.93024552831743	40.0	37.0	41.0	31.0	41.0
18	36.90737910469561	40.0	37.0	41.0	31.0	41.0
19	36.940463015691876	40.0	37.0	41.0	31.0	41.0
20	36.91366905935041	40.0	37.0	41.0	31.0	41.0
21	36.837715668539644	40.0	37.0	41.0	31.0	41.0
22	36.76800596432758	40.0	37.0	41.0	30.0	41.0
23	36.67362413181744	40.0	37.0	41.0	30.0	41.0
24	36.60611103483134	40.0	37.0	41.0	30.0	41.0
25	36.321891868301606	40.0	36.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9009048162891524
1101	2	0.9947726688897482
1101	3	1.0136147729240363
1101	4	0.8003846945970778
1101	5	0.9377004399141455
1101	6	0.9211286181944374
1101	7	0.9791968639264823
1101	8	1.0065739974078554
1101	9	1.1140656090157606
1101	10	1.0454060128815073
1101	11	1.1641227483943197
1101	12	1.1888610340646935
1101	13	1.227790754506735
1101	14	1.409755146423329
1101	15	1.4136353270952569
1101	16	1.3913346474244292
1101	17	1.3672855769917618
1101	18	1.413244278053618
1101	19	1.4877379825289552
1101	20	1.4679952680437083
1101	21	1.4153721180665073
1101	22	1.382180172765608
1101	23	1.480424488782866
1101	24	1.4764905210459673
1101	25	1.389852557102067
1102	1	0.8031191036234482
1102	2	0.8766653664618431
1102	3	0.8336332997375955
1102	4	0.7315995404290447
1102	5	0.8200386598658369
1102	6	0.7144741326359991
1102	7	0.787520888965318
1102	8	0.83210825571161
1102	9	0.8980370093990544
1102	10	0.9508869445803612
1102	11	0.9643834278903682
1102	12	0.9752411328365653
1102	13	0.9723916838241706
1102	14	1.1102340510874953
1102	15	1.1608008805466028
1102	16	1.1731759603759073
1102	17	1.2398962984460127
1102	18	1.1731389714752538
1102	19	1.1302334181571396
1102	20	1.2194473619847201
1102	21	1.1819939442688394
1102	22	1.2414498165745087
1102	23	1.2576093628515608
1102	24	1.278645414846359
1102	25	1.2787755212788312
1103	1	0.6187453751055578
1103	2	0.6845391001448
1103	3	0.6380076147409248
1103	4	0.531557053557485
1103	5	0.5464291052182233
1103	6	0.5050736373890317
1103	7	0.6308734596876349
1103	8	0.5799158165086595
1103	9	0.7029057049721246
1103	10	0.7500989547358898
1103	11	0.7215382003989959
1103	12	0.7313170720380171
1103	13	0.7588387716740286
1103	14	0.8755568330145493
1103	15	0.8898580352191416
1103	16	0.9173811009874555
1103	17	0.9170103701978434
1103	18	0.9188960991894533
1103	19	0.9429557202991106
1103	20	0.9590062716149745
1103	21	0.8937224201697873
1103	22	0.9618635753896996
1103	23	1.0457144717062548
1103	24	0.9993474845734767
1103	25	1.053065365716904
1104	1	0.5906719217387071
1104	2	0.6034232616825221
1104	3	0.5813523122891837
1104	4	0.527453544099636
1104	5	0.5225827939770653
1104	6	0.4859229659589346
1104	7	0.5813169970869154
1104	8	0.6282492090806429
1104	9	0.6329483471953239
1104	10	0.7004057949429878
1104	11	0.7044752911919971
1104	12	0.7548870749935475
1104	13	0.6933091348966229
1104	14	0.9040938528492504
1104	15	0.9093528351862261
1104	16	0.9693750259836378
1104	17	0.9017979804177543
1104	18	0.929977665689961
1104	19	0.9186408473307992
1104	20	0.9467571593141813
1104	21	0.9654477154904768
1104	22	0.9608333671994558
1104	23	0.9968571212622379
1104	24	0.9481266399968078
1104	25	0.9290793793405143
1105	1	0.5397359187245492
1105	2	0.5395194686135412
1105	3	0.5086590257060202
1105	4	0.46177678154898416
1105	5	0.3991280486040196
1105	6	0.4374168953569537
1105	7	0.5420909892285835
1105	8	0.5484166792658769
1105	9	0.6156057253411547
1105	10	0.6349158792932172
1105	11	0.624093600650454
1105	12	0.6737995183820118
1105	13	0.6681644631089654
1105	14	0.8079059909775523
1105	15	0.7867094513350281
1105	16	0.7659065938828817
1105	17	0.635791506134197
1105	18	0.8358746461517796
1105	19	0.7602789487202273
1105	20	0.7796718940761664
1105	21	0.8100327692762406
1105	22	0.7203063512905601
1105	23	0.759311197677242
1105	24	0.7768197112831317
1105	25	0.7529800967403375
1106	1	0.4570194135714978
1106	2	0.5190922815873691
1106	3	0.47051148796457554
1106	4	0.45680260175246445
1106	5	0.45614213434907214
1106	6	0.4859004253633259
1106	7	0.5657519644631606
1106	8	0.5428425917628559
1106	9	0.5705607871997742
1106	10	0.5597590902278
1106	11	0.5887016829235066
1106	12	0.647309527831105
1106	13	0.6622875650493256
1106	14	0.6941098716991974
1106	15	0.6950061968906525
1106	16	0.7160773368455011
1106	17	0.7107571158206198
1106	18	0.6987511266932316
1106	19	0.7063808671064109
1106	20	0.7111694658783065
1106	21	0.7131448179720437
1106	22	0.699613710597923
1106	23	0.8027889044036769
1106	24	0.7357825082154719
1106	25	0.7779182381251815
1107	1	0.4040037593877983
1107	2	0.4395267736732684
1107	3	0.49268575617309196
1107	4	0.41370075705758325
1107	5	0.34136784907647666
1107	6	0.35276586324710735
1107	7	0.4197938289084533
1107	8	0.4419040672315475
1107	9	0.4826872675300393
1107	10	0.47068897289955913
1107	11	0.4744256350663605
1107	12	0.4784874648173343
1107	13	0.4925163335716718
1107	14	0.5217251323952965
1107	15	0.6064340635508998
1107	16	0.5329142641955968
1107	17	0.5709023463673546
1107	18	0.543220294346149
1107	19	0.5548983615922651
1107	20	0.5139917130490019
1107	21	0.5226882017120005
1107	22	0.5967513374909146
1107	23	0.5539723660977671
1107	24	0.562255151196851
1107	25	0.4869346627268598
1108	1	0.20168449989073522
1108	2	0.26960382598396393
1108	3	0.2791227266342702
1108	4	0.112622789656605
1108	5	0.1267360884858988
1108	6	0.13577610745699076
1108	7	0.18798563248263633
1108	8	0.22820245176558274
1108	9	0.2647921053832789
1108	10	0.23587143555439383
1108	11	0.3455644036860477
1108	12	0.3673911101643128
1108	13	0.38699848971805295
1108	14	0.37040093694437815
1108	15	0.2914239381522066
1108	16	0.3982930501324162
1108	17	0.3882160660893206
1108	18	0.38397570564647765
1108	19	0.35401427614748826
1108	20	0.30464908477462416
1108	21	0.3644548101530489
1108	22	0.28942626153516215
1108	23	0.336307990615353
1108	24	0.39924628226715697
1108	25	0.34521456154204344
1109	1	0.12055781013467737
1109	2	0.15036771946179073
1109	3	0.10734270556617176
1109	4	0.06333756310976213
1109	5	0.04599953429903536
1109	6	0.06582989200524025
1109	7	0.020317129807665424
1109	8	0.04563675648595478
1109	9	0.11698825378250888
1109	10	0.15774934683256703
1109	11	0.134632833542625
1109	12	0.20348065865138665
1109	13	0.14219470419803315
1109	14	0.21050793593243355
1109	15	0.19965461957431074
1109	16	0.13238151800486264
1109	17	0.14482887046568038
1109	18	0.15549308812315843
1109	19	0.20065238556425413
1109	20	0.16905255051564438
1109	21	0.20669647652818668
1109	22	0.16792319655446164
1109	23	0.1408938651422602
1109	24	0.22565927659328366
1109	25	0.21642939783669846
1110	1	0.05887738723092539
1110	2	0.11892776035767838
1110	3	0.08438416888003175
1110	4	0.08238116460761802
1110	5	-0.1132896234261338
1110	6	-0.06383390264304012
1110	7	0.007504527968279717
1110	8	-0.007548259992930184
1110	9	0.0834787350010302
1110	10	0.07125286129220143
1110	11	0.13083519776879626
1110	12	0.06263469468758842
1110	13	0.1503154516969829
1110	14	0.16352815137904742
1110	15	0.06412509632871632
1110	16	0.1049735979229709
1110	17	0.13708610676141575
1110	18	0.07399243794018417
1110	19	0.0863900675977618
1110	20	0.12016052636242591
1110	21	0.08834366541275784
1110	22	0.10680526321466033
1110	23	0.1152209642651556
1110	24	0.09793788983941454
1110	25	0.10070548222675768
1111	1	-0.04978429457413469
1111	2	-0.11283375803177975
1111	3	-0.11296078369404938
1111	4	-0.12410575336436835
1111	5	-0.21830923496992938
1111	6	-0.22955471573256858
1111	7	-0.13320182276811465
1111	8	-0.1396056095960745
1111	9	-0.07804948947529056
1111	10	-0.11263166918331535
1111	11	-0.10067174566022885
1111	12	-0.136845230645946
1111	13	-0.08699076427492969
1111	14	-0.1699181381462651
1111	15	-0.17610264203535309
1111	16	-0.16442323409768278
1111	17	-0.14739648943432826
1111	18	-0.16788893878197086
1111	19	-0.2027032886344955
1111	20	-0.1916833607627879
1111	21	-0.15692287148020512
1111	22	-0.1654004326207783
1111	23	-0.24139242503621006
1111	24	-0.21160229993476776
1111	25	-0.1997907947174653
1112	1	-0.3204472596049399
1112	2	-0.45168353027234787
1112	3	-0.2910616771799397
1112	4	-0.25887800716759557
1112	5	-0.304657502287327
1112	6	-0.28906772853950713
1112	7	-0.2755692034884447
1112	8	-0.2605169927164681
1112	9	-0.3376034030413919
1112	10	-0.3152422536454438
1112	11	-0.3388614778860912
1112	12	-0.33547237362378013
1112	13	-0.26770586106797367
1112	14	-0.40334179941334725
1112	15	-0.4305291208379671
1112	16	-0.48264378898985427
1112	17	-0.42662327329603045
1112	18	-0.4761451731428892
1112	19	-0.43994389848017335
1112	20	-0.4823921237092179
1112	21	-0.45126527592154275
1112	22	-0.5054965585170095
1112	23	-0.44632998325394624
1112	24	-0.4513482656927721
1112	25	-0.3656735084711187
1113	1	-0.508015898983178
1113	2	-0.5390102091708648
1113	3	-0.4677161242945047
1113	4	-0.4325716728222275
1113	5	-0.5757595887668998
1113	6	-0.5990972679556776
1113	7	-0.5842432103981778
1113	8	-0.6372744437200879
1113	9	-0.7081570543340092
1113	10	-0.6510134589928285
1113	11	-0.6069780972908276
1113	12	-0.6506874259541746
1113	13	-0.6658568069498472
1113	14	-0.7023665497373202
1113	15	-0.6495886250256291
1113	16	-0.7076202547485053
1113	17	-0.7516211456903434
1113	18	-0.7337512961039465
1113	19	-0.8130110884127859
1113	20	-0.6971864469380549
1113	21	-0.8736141351870472
1113	22	-0.7354927228160122
1113	23	-0.7041081800814908
1113	24	-0.689255413042595
1113	25	-0.7219190788955743
1114	1	-0.6119948968087634
1114	2	-1.272772446811178
1114	3	-0.8031702611629932
1114	4	-0.6544945993591753
1114	5	-0.8205712796878117
1114	6	-0.7159311054882096
1114	7	-0.7152032356536111
1114	8	-0.7425124596995829
1114	9	-0.7822370448285128
1114	10	-0.8125121712789891
1114	11	-0.8439137306485947
1114	12	-0.849981947976076
1114	13	-0.83657702506612
1114	14	-0.9987101624490435
1114	15	-1.0478661735697656
1114	16	-0.9784221647591593
1114	17	-1.0701145382565258
1114	18	-1.0733648544157575
1114	19	-1.0218535650013862
1114	20	-1.1000273359408865
1114	21	-1.1299877455853604
1114	22	-1.114924692731627
1114	23	-1.220168683017171
1114	24	-1.2351485115647733
1114	25	-1.2447661921580462
1115	1	-0.7828330444497951
1115	2	-1.1350469272476964
1115	3	-1.0515864705764706
1115	4	-0.9165126065666414
1115	5	-0.9892507829530572
1115	6	-0.930339957282186
1115	7	-1.0320938524618626
1115	8	-1.1104950760191983
1115	9	-1.5013115334090656
1115	10	-1.271607522566505
1115	11	-1.37319946129508
1115	12	-1.2449395863100108
1115	13	-1.6458405696012193
1115	14	-1.5114961259638022
1115	15	-1.6512151502218586
1115	16	-1.605975285448551
1115	17	-1.5333315307691109
1115	18	-1.534394412144188
1115	19	-1.4983001478185116
1115	20	-1.6202886255984836
1115	21	-1.5093610245233862
1115	22	-1.6937065435126897
1115	23	-1.6750130748364924
1115	24	-1.5909941415307216
1115	25	-1.4770033055076368
1116	1	-0.812445717357658
1116	2	-0.7220353309527319
1116	3	-0.8524446634665033
1116	4	-0.8700752542418684
1116	5	-0.9897733113489622
1116	6	-1.0474195046993344
1116	7	-1.2683444771533487
1116	8	-1.198723446382857
1116	9	-1.2433667362316854
1116	10	-1.2590255011873595
1116	11	-1.3375837022888035
1116	12	-1.4226372889152188
1116	13	-1.3016819938120605
1116	14	-1.47763099593611
1116	15	-1.616517519272442
1116	16	-1.660373856068695
1116	17	-1.8587178595797553
1116	18	-1.6330518766561966
1116	19	-1.601338261348225
1116	20	-1.5640805166743377
1116	21	-1.6116322833257115
1116	22	-1.7244689626243357
1116	23	-1.933068264797825
1116	24	-1.7381192439617479
1116	25	-1.9850800457939854
1201	1	0.7153065243871062
1201	2	0.8466679228364953
1201	3	0.7798509925557084
1201	4	0.7349574096404723
1201	5	0.7819680671580613
1201	6	0.7521020250767947
1201	7	0.722288763556584
1201	8	0.7535090310874466
1201	9	0.8760828500179443
1201	10	0.8443940998279231
1201	11	0.8936878407757476
1201	12	0.9751942717489896
1201	13	0.9299600616431647
1201	14	1.1493426290251847
1201	15	1.1428390013996506
1201	16	1.216914628820355
1201	17	1.1912763765524517
1201	18	1.0808812139881425
1201	19	1.1347537614536307
1201	20	1.0918683459010623
1201	21	1.1457570387516824
1201	22	1.196936535393064
1201	23	1.2237550193212883
1201	24	1.232213777403409
1201	25	1.2668330893097917
1202	1	0.7321663954239455
1202	2	0.7884498133117042
1202	3	0.7199655198486639
1202	4	0.664956007417409
1202	5	0.8089580691834897
1202	6	0.8078703116443151
1202	7	0.7837272638038684
1202	8	0.7958492393582475
1202	9	0.8798808477206137
1202	10	0.8855029508060781
1202	11	0.9184115359603027
1202	12	0.9132394514589777
1202	13	0.9710741519995523
1202	14	1.0775580802549172
1202	15	1.0596484985723862
1202	16	1.082218797476493
1202	17	1.1592213435023169
1202	18	1.1156395249173912
1202	19	1.186825287514914
1202	20	1.159636428684344
1202	21	1.1600003574069433
1202	22	1.1595818712640948
1202	23	1.1835668856581307
1202	24	1.207407768829306
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2211	4	-0.285294877426729
2211	5	-0.3090143736913262
2211	6	-0.35555194489824515
2211	7	-0.3983515167911378
2211	8	-0.3776745608283605
2211	9	-0.43437811390805336
2211	10	-0.48750966118329586
2211	11	-0.4655990854740182
2211	12	-0.5155641114913649
2211	13	-0.4679625383253807
2211	14	-0.5599699411862389
2211	15	-0.5221636445845448
2211	16	-0.5360805943411862
2211	17	-0.5863009541291788
2211	18	-0.5543502414044426
2211	19	-0.5550980381110335
2211	20	-0.5148921226167857
2211	21	-0.5468432083193804
2211	22	-0.5289719965485062
2211	23	-0.5718197303652346
2211	24	-0.5932055358335688
2211	25	-0.5911266810395972
2212	1	-0.4215602546884121
2212	2	-0.3849725843456646
2212	3	-0.46794096797426477
2212	4	-0.39887983677186867
2212	5	-0.4612182717422968
2212	6	-0.43957365667009896
2212	7	-0.48140823660641274
2212	8	-0.38067612573934184
2212	9	-0.40366624356585135
2212	10	-0.5180983084654969
2212	11	-0.5470215129464151
2212	12	-0.6318155478169984
2212	13	-0.5251119927952246
2212	14	-0.6765873259245225
2212	15	-0.6644543240623264
2212	16	-0.6575837843520205
2212	17	-0.680455847485419
2212	18	-0.6721545509285178
2212	19	-0.6493971659193889
2212	20	-0.5735944502850714
2212	21	-0.603569151916922
2212	22	-0.6340602553388166
2212	23	-0.6042954633016819
2212	24	-0.6819795429905753
2212	25	-0.5807444269111883
2213	1	-0.7403461071566362
2213	2	-0.6416780866689287
2213	3	-0.6170252750195786
2213	4	-0.6642119107228552
2213	5	-0.6965667719596595
2213	6	-0.7272812881674895
2213	7	-0.7590873204586401
2213	8	-0.7121120225785802
2213	9	-0.8692330237955233
2213	10	-0.8192876209210098
2213	11	-0.9369396143111786
2213	12	-0.9233451579338023
2213	13	-0.9034464772717143
2213	14	-1.061053937660688
2213	15	-1.0440257391856989
2213	16	-1.093228972767541
2213	17	-1.066755474007337
2213	18	-1.1365699270984777
2213	19	-1.1161570029048278
2213	20	-1.0385953123202967
2213	21	-1.1283533733933382
2213	22	-1.0403611722693
2213	23	-1.141996336019801
2213	24	-1.134194402362482
2213	25	-0.9339221214587923
2214	1	-0.8873849644449656
2214	2	-1.4478429966820556
2214	3	-0.9956102409948571
2214	4	-0.8798845561002011
2214	5	-0.9551045070874977
2214	6	-0.9568635557016165
2214	7	-0.9407537850447625
2214	8	-0.9637153224709891
2214	9	-1.169574605978994
2214	10	-1.223918052732202
2214	11	-1.1018864780347926
2214	12	-1.1788085422503656
2214	13	-1.1648580766612895
2214	14	-1.358962520450234
2214	15	-1.4087915420990953
2214	16	-1.4211427093974223
2214	17	-1.3865861567297912
2214	18	-1.3358665609693716
2214	19	-1.3802213210201586
2214	20	-1.3464150281201839
2214	21	-1.3844534739638021
2214	22	-1.3543883157445293
2214	23	-1.3609945958200456
2214	24	-1.3985820703192573
2214	25	-1.3158823882923585
2215	1	-1.050774207664972
2215	2	-1.2819288987644732
2215	3	-1.311949645354911
2215	4	-1.004579240987944
2215	5	-1.189014395216816
2215	6	-1.0549871160420352
2215	7	-1.0723778919739715
2215	8	-1.1404849718468313
2215	9	-1.305626409358318
2215	10	-1.2109219679516627
2215	11	-1.4408797509560642
2215	12	-1.5221925632382138
2215	13	-1.507285949125361
2215	14	-1.662346570531291
2215	15	-1.8591492795488307
2215	16	-1.741678082747022
2215	17	-1.6879588473649036
2215	18	-1.7669394241999825
2215	19	-1.6554768867340997
2215	20	-1.692839581048375
2215	21	-1.6660654708420992
2215	22	-1.7656889933982498
2215	23	-1.6676922176678843
2215	24	-1.8495189082213201
2215	25	-1.8728607960352264
2216	1	-1.1002784649558954
2216	2	-1.1121140279564337
2216	3	-1.182755500481644
2216	4	-1.0687484947476165
2216	5	-1.078671852644156
2216	6	-1.0533000344162389
2216	7	-1.1658827309411492
2216	8	-1.1701904486334485
2216	9	-1.3643418769657956
2216	10	-1.34380620926553
2216	11	-1.4571456198991157
2216	12	-1.573615450194481
2216	13	-1.4212514808986256
2216	14	-1.6253706416457234
2216	15	-1.6908869752269169
2216	16	-1.70030131798746
2216	17	-1.8098112637516977
2216	18	-1.824731245234517
2216	19	-1.6784936277655973
2216	20	-1.80573759057107
2216	21	-1.7676742907538667
2216	22	-1.815957046533633
2216	23	-1.8265812160191643
2216	24	-1.929591796144713
2216	25	-1.8691174427437716
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54325.0
3	3.0
4	1019.0
5	7840.0
6	9600.0
7	7999.0
8	6523.0
9	5544.0
10	5135.0
11	4772.0
12	4475.0
13	4223.0
14	4232.0
15	4319.0
16	4402.0
17	4828.0
18	5411.0
19	6800.0
20	9090.0
21	11795.0
22	15187.0
23	18176.0
24	22231.0
25	25276.0
26	28182.0
27	31736.0
28	37068.0
29	44863.0
30	57882.0
31	75746.0
32	99235.0
33	131098.0
34	177294.0
35	244169.0
36	354351.0
37	617789.0
38	1855821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34573647811713	18.610871564438085	13.146326997997186	25.8970649594476
2	16.501940967865295	21.41282351861296	36.81788816793692	25.267347345584824
3	17.911965144397602	25.185678711117017	29.533500448550047	27.36885569593534
4	12.989815518828285	17.414837528512546	36.89340809956381	32.70193885309536
5	15.098806822722398	36.06960705990988	33.40936459658714	15.422221520780585
6	31.941526393814858	37.45488437263475	16.686895982285275	13.916693251265114
7	28.34302812698988	31.388347139806612	21.969324469045606	18.299300264157907
8	26.511730273120726	32.943904021968706	20.313524233977713	20.230841470932855
9	27.05454861942166	15.055097834576694	19.364686115785858	38.52566743021579
10	16.013932792558506	26.844960792449463	31.49041714092634	25.65068927406569
11	35.27158935476473	22.434411414048373	23.28265686200705	19.011342369179847
12	24.596788413176984	24.43664675421144	28.861638657091014	22.10492617552056
13	29.062306867519027	20.671712736670532	25.198369177137764	25.067611218672674
14	23.501136060077616	20.71446222987933	25.970528470535974	29.81387323950708
15	24.516877734745762	27.464119260238373	23.188067973633895	24.83093503138197
16	24.747900168466714	26.719405884730353	24.156741069463507	24.375952877339426
17	24.160742463946423	25.921433103885377	25.444086973290375	24.473737458877824
18	25.12391138304061	24.77217262209942	26.509232892376044	23.59468310248393
19	26.110975918111222	25.011185727087266	25.736701114621123	23.14113724018038
20	25.212354555699896	24.987657149374254	25.644616941444117	24.155371353481733
21	25.693473193473192	24.91813980011405	25.54043238592595	23.847954620486806
22	24.654660482835062	24.85961828578746	25.948858338768865	24.536862892608614
23	23.956684218871846	24.27346425707753	26.330133519809884	25.439718004240742
24	24.23009327193071	25.47116793624505	25.984109782410513	24.31462900941373
25	24.35869762863002	24.909902147316522	26.11140694215554	24.619993281897916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1183.0
1	1183.0
2	1860.5
3	2538.0
4	2538.0
5	2538.0
6	5928.5
7	9319.0
8	9319.0
9	9319.0
10	11160.0
11	13001.0
12	13001.0
13	13001.0
14	16225.5
15	19450.0
16	19450.0
17	19450.0
18	33381.0
19	47312.0
20	47312.0
21	47312.0
22	68520.0
23	89728.0
24	89728.0
25	89728.0
26	123331.0
27	156934.0
28	156934.0
29	156934.0
30	185372.5
31	213811.0
32	213811.0
33	213811.0
34	252024.5
35	290238.0
36	290238.0
37	290238.0
38	317662.5
39	345087.0
40	345087.0
41	345087.0
42	380446.5
43	415806.0
44	415806.0
45	415806.0
46	443725.5
47	471645.0
48	471645.0
49	471645.0
50	477491.0
51	483337.0
52	483337.0
53	483337.0
54	453026.0
55	422715.0
56	422715.0
57	422715.0
58	386902.5
59	351090.0
60	351090.0
61	351090.0
62	307822.0
63	264554.0
64	264554.0
65	264554.0
66	218039.5
67	171525.0
68	171525.0
69	171525.0
70	133499.0
71	95473.0
72	95473.0
73	95473.0
74	76722.0
75	57971.0
76	57971.0
77	57971.0
78	49111.5
79	40252.0
80	40252.0
81	40252.0
82	30303.0
83	20354.0
84	20354.0
85	20354.0
86	15279.0
87	10204.0
88	10204.0
89	10204.0
90	7328.0
91	4452.0
92	4452.0
93	4452.0
94	2420.5
95	389.0
96	389.0
97	389.0
98	230.0
99	71.0
100	71.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.43867119143245653
2	0.004401717770359883
3	0.0
4	0.004976942251713731
5	0.1665650019920274
6	0.04411721674383428
7	0.0018257124842969968
8	6.002342414127114E-4
9	1.5005856035317785E-4
10	0.0025509955260040232
11	0.0017006636840026822
12	0.002601015046121749
13	0.00530206913247895
14	0.01027901138419268
15	0.003101210247299008
16	5.752244813538483E-4
17	0.007302849937187987
18	4.2516592100067056E-4
19	0.001000390402354519
20	0.0043266884901832945
21	0.003976551849359212
22	0.0013755368032374634
23	0.002025790564767901
24	0.0032512688076521863
25	0.007152791376834809
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3998439.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.15338280291818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.49615302537616	45.4440974993469
2	8.351759705871329	8.878485614483322
3	2.307759023993668	3.6799559645767284
4	1.0623808399539596	2.258765418742343
5	0.6008391777342668	1.5968317408550041
6	0.3871988622479188	1.2348557607550807
7	0.271997251790392	1.0120301832029504
8	0.20367301570582813	0.866072781634932
9	0.16815728632082813	0.8044315749819805
>10	0.9572799921448761	9.628555749559105
>50	0.0957619323606738	3.5306503660317032
>100	0.07675686869590857	8.426346342449103
>500	0.012499999361043646	4.6281299882259646
>1k	0.007547169399325832	6.859493820962176
>5k	1.4150942623735935E-4	0.45568994325769974
>10k+	9.433961749157291E-5	0.6956072509348898
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	16310	0.40790918656005504	No Hit
TATCAACGCAGAGTACTTTTTTTTT	11434	0.2859615965130392	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6378	0.15951224965542804	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6325	0.1581867323723083	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5472	0.13685340704209817	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4202	0.1050910117673422	No Hit
CTATTGGAGCTGGAATTACCGCGGC	4152	0.10384052376439905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5009760058862972E-5
2	0.0	0.0	0.0	2.5009760058862972E-5	2.5009760058862972E-5
3	0.0	0.0	0.0	2.5009760058862972E-5	2.5009760058862972E-5
4	0.0	0.0	0.0	2.5009760058862972E-5	2.5009760058862972E-5
5	0.0	0.0	0.0	2.5009760058862972E-5	2.5009760058862972E-5
6	0.0	0.0	0.0	2.5009760058862972E-5	2.5009760058862972E-5
7	0.0	0.0	0.0	2.5009760058862972E-5	2.5009760058862972E-5
8	0.0	0.0	0.0	2.5009760058862972E-5	2.5009760058862972E-5
9	0.0	0.0	0.0	5.0019520117725944E-5	2.5009760058862972E-5
10	2.5009760058862972E-5	0.0	0.0	5.0019520117725944E-5	2.5009760058862972E-5
11	2.5009760058862972E-5	0.0	0.0	1.0003904023545189E-4	2.5009760058862972E-5
12	2.5009760058862972E-5	0.0	0.0	1.0003904023545189E-4	1.0003904023545189E-4
13	2.5009760058862972E-5	0.0	0.0	1.2504880029431487E-4	1.750683204120408E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2360	0.0	15.926822	1
GTATCAA	7095	0.0	12.760235	1
TACCGAA	55	0.0030856512	12.082648	7
ATACCGT	610	0.0	11.521326	6
CGTCGTA	595	0.0	11.328814	10
CGCGCTA	235	0.0	11.311415	16
TACCGTC	610	0.0	11.049822	7
GCGCGCT	245	0.0	10.849725	15
CGAGCCG	765	0.0	10.79654	15
TAGATCG	115	8.6930595E-7	10.751965	5
AGGCCCG	820	0.0	10.535875	10
ACCGTCG	675	0.0	10.40809	8
CCGTCGT	665	0.0	10.279072	9
ATCGCCA	435	0.0	10.257354	16
ACGCATC	260	0.0	10.224162	10
CGAACGA	270	0.0	10.196732	16
GCTCGTA	425	0.0	9.828943	9
CGACCAT	615	0.0	9.725423	10
CGGTTCT	755	0.0	9.6823635	12
GCCGCCT	880	0.0	9.60127	18
>>END_MODULE