FastQCFastQC Report
Thu 2 Feb 2017
SRR4050265_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050265_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594478
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93750.26081673055169624No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN80550.2240937348900174No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN61190.17023334125288847No Hit
TATCAACGCAGAGTACTTTTTTTTT53180.14794915979455153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15300.015.7111551
GTATCAA52250.013.2926041
TAGATCG701.0903662E-412.2158435
CGAACGA1950.011.69216116
ACGCTTA1604.5474735E-1111.28047717
ATACCGT3800.011.2504896
CGTTAAG600.005872609611.0847472
TACCGTC3750.010.8927397
CGTCGTA3500.010.8565510
ACCGTCG3900.010.7173638
ATTATAC4750.010.6013523
GTATTAG4000.010.4513321
CGCTTAC1752.3646862E-1010.31372318
CCGTCGT3700.010.2694239
GCGTTAT2151.8189894E-1210.1640871
TATCACG750.002646208510.1346262
CGGTTCT4900.010.08108312
CTTACAC4450.010.0349873
CGACCAT3700.010.01296710
CGTTATT2002.5465852E-119.9762722
Produced by FastQC (version 0.11.5)