##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050265_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3594478
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.96334989392062	31.0	30.0	34.0	26.0	34.0
2	30.01562758208563	31.0	31.0	34.0	26.0	34.0
3	30.152684757007833	31.0	31.0	34.0	26.0	34.0
4	33.557927743611174	37.0	35.0	37.0	28.0	37.0
5	33.669961813648605	37.0	35.0	37.0	28.0	37.0
6	33.741127640786786	37.0	35.0	37.0	29.0	37.0
7	33.672898818688	37.0	35.0	37.0	29.0	37.0
8	33.60510538665142	37.0	35.0	37.0	28.0	37.0
9	35.088385017240334	39.0	35.0	39.0	28.0	39.0
10	34.98420632982035	39.0	35.0	39.0	28.0	39.0
11	35.01223932932682	39.0	35.0	39.0	28.0	39.0
12	34.90391149980609	39.0	35.0	39.0	27.0	39.0
13	34.89258162103093	39.0	35.0	39.0	27.0	39.0
14	35.74632950876316	40.0	36.0	41.0	27.0	41.0
15	35.87651085915674	40.0	36.0	41.0	27.0	41.0
16	35.90391817671439	40.0	36.0	41.0	27.0	41.0
17	35.895550063180245	40.0	36.0	41.0	27.0	41.0
18	35.72421725769361	40.0	36.0	41.0	27.0	41.0
19	35.853565107367466	40.0	36.0	41.0	27.0	41.0
20	35.82180277636975	40.0	36.0	41.0	27.0	41.0
21	35.78862827926614	40.0	36.0	41.0	27.0	41.0
22	35.71769280546438	40.0	36.0	41.0	27.0	41.0
23	35.611675464420706	40.0	36.0	41.0	27.0	41.0
24	35.54780193396649	40.0	36.0	41.0	27.0	41.0
25	35.26699871302593	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1810460597961026
1101	2	1.13245555783541
1101	3	1.0652300342207006
1101	4	1.2113857256536775
1101	5	1.1418697126025634
1101	6	1.1277399081943642
1101	7	1.2118923881810204
1101	8	1.1997780695880067
1101	9	1.3679352058381866
1101	10	1.4326927543376726
1101	11	1.3305014856752422
1101	12	1.3277149801213923
1101	13	1.3715998768887445
1101	14	1.7673901132670835
1101	15	1.683782803025231
1101	16	1.675997643371268
1101	17	1.648269161675394
1101	18	1.7353531810112415
1101	19	1.6278534942188898
1101	20	1.665421983009189
1101	21	1.638919371346418
1101	22	1.669485922218982
1101	23	1.6577908720971166
1101	24	1.7519750308446191
1101	25	1.7478693259744134
1102	1	0.9708973574315429
1102	2	1.0284899584587102
1102	3	0.9323428462588659
1102	4	1.0430192628685901
1102	5	0.9662892729852999
1102	6	0.9810814921374416
1102	7	0.967473555076829
1102	8	0.9477031508500744
1102	9	1.0570806953018703
1102	10	1.1181022417385265
1102	11	1.106007213950754
1102	12	1.193995684586362
1102	13	1.143058595827597
1102	14	1.5591120652827044
1102	15	1.427159348535639
1102	16	1.365916692745131
1102	17	1.3019171073570845
1102	18	1.4068909610439277
1102	19	1.22646098198652
1102	20	1.2736298955910073
1102	21	1.3373871624761975
1102	22	1.4008464515942478
1102	23	1.341189817090843
1102	24	1.3395398030028716
1102	25	1.3286789331500444
1103	1	0.7863837942900993
1103	2	0.7780257543100042
1103	3	0.7954651683504252
1103	4	0.8961744584469287
1103	5	0.825742517970987
1103	6	0.7720604084926492
1103	7	0.8240308588154548
1103	8	0.9032311559618975
1103	9	1.025406669734437
1103	10	1.0248224671865955
1103	11	1.014547214409788
1103	12	1.0273079854878517
1103	13	1.0343562903381809
1103	14	1.4223595609735042
1103	15	1.2827600646021438
1103	16	1.2603313528156974
1103	17	1.1646540254499342
1103	18	1.3394348471528588
1103	19	1.2379249642124392
1103	20	1.1871620152182487
1103	21	1.2021562218821558
1103	22	1.234846206719567
1103	23	1.1207986824691503
1103	24	1.1635774140551334
1103	25	1.1870426573264083
1104	1	0.7362298218942271
1104	2	0.6641087411376851
1104	3	0.6637259329266954
1104	4	0.7652827984055861
1104	5	0.758559430756172
1104	6	0.7314138746997045
1104	7	0.724279298337656
1104	8	0.774354539659285
1104	9	0.8475388365625847
1104	10	0.9221589187649926
1104	11	0.9454997008581856
1104	12	0.9996714885156308
1104	13	1.0108652679117966
1104	14	1.2933303185218676
1104	15	1.2059743164464152
1104	16	1.091670920965207
1104	17	1.0413342216720736
1104	18	1.2980199675983002
1104	19	1.2185621102031163
1104	20	1.1477048142211217
1104	21	1.0717456953984268
1104	22	1.038020938782644
1104	23	1.0109922611768667
1104	24	1.0526358575086903
1104	25	1.182690629498147
1105	1	0.6193883794761561
1105	2	0.6181419959614196
1105	3	0.568140642065039
1105	4	0.6932544763531894
1105	5	0.6664159403092
1105	6	0.5799004797202443
1105	7	0.6820046210399084
1105	8	0.6275035334621251
1105	9	0.7761232626611019
1105	10	0.7941860956327389
1105	11	0.6659204179029103
1105	12	0.689341729620871
1105	13	0.7197438993834169
1105	14	1.0935083129361942
1105	15	0.9680994980678648
1105	16	0.9477693973267733
1105	17	0.9119617821171886
1105	18	0.9806343468193575
1105	19	0.887585638765394
1105	20	0.9400435735236101
1105	21	0.8951748050850341
1105	22	0.8700855215453984
1105	23	0.820890419310949
1105	24	0.8581074307908736
1105	25	0.8261232595121157
1106	1	0.44319722501519365
1106	2	0.3990744798491477
1106	3	0.4077423460364997
1106	4	0.5973727159909998
1106	5	0.4691833449290712
1106	6	0.3674674917842822
1106	7	0.4018608097230896
1106	8	0.47693227286438145
1106	9	0.6596274786123857
1106	10	0.6408458992195136
1106	11	0.6047013654138809
1106	12	0.6146168552056182
1106	13	0.6845323469041844
1106	14	0.885046427421905
1106	15	0.7880406146940473
1106	16	0.6521556318069486
1106	17	0.6657345234879202
1106	18	0.9031851193075511
1106	19	0.7941574591397682
1106	20	0.77199364372715
1106	21	0.7966322540490367
1106	22	0.790481616925355
1106	23	0.8377951750264927
1106	24	0.7996616940489787
1106	25	0.8971423200488218
1107	1	0.2757835840552012
1107	2	0.3151598419785273
1107	3	0.3051542345271123
1107	4	0.4136659643703098
1107	5	0.2877673014678308
1107	6	0.2848821828487189
1107	7	0.2841628658964481
1107	8	0.30086132987403147
1107	9	0.3163449967301304
1107	10	0.45089254107158183
1107	11	0.3707624374224707
1107	12	0.3355623072721414
1107	13	0.21035847784084183
1107	14	0.6291140431305067
1107	15	0.5500081730084148
1107	16	0.5031290097657006
1107	17	0.4943424671665184
1107	18	0.4868429119604656
1107	19	0.48621928928933755
1107	20	0.42151384928595803
1107	21	0.3482808319494026
1107	22	0.41746341362306794
1107	23	0.4198644523101862
1107	24	0.5504664011335336
1107	25	0.48744865100800183
1108	1	0.4092293215752143
1108	2	0.4232461471737743
1108	3	0.3353470512780241
1108	4	0.49119080596829434
1108	5	0.3170007258532621
1108	6	0.3903642722337324
1108	7	0.4564453210382595
1108	8	0.3464793811251141
1108	9	0.4660509832197235
1108	10	0.47715079339652533
1108	11	0.4563472306179932
1108	12	0.3569309576815698
1108	13	0.44875058902267284
1108	14	0.7372635600098079
1108	15	0.5684517984135979
1108	16	0.568150078787454
1108	17	0.5584626707160609
1108	18	0.742471424151411
1108	19	0.5984059081280293
1108	20	0.6489638068127164
1108	21	0.6160641327949179
1108	22	0.6367425021818462
1108	23	0.743386800015891
1108	24	0.6855906793953253
1108	25	0.6089048872101372
1109	1	0.0956924352365327
1109	2	0.16574293450056743
1109	3	0.18961447235612638
1109	4	0.29805789056944576
1109	5	0.21743121674285248
1109	6	0.1998789280004729
1109	7	0.20038487536128713
1109	8	0.21902468333409075
1109	9	0.2533172763215603
1109	10	0.21145312375181646
1109	11	0.21670376939536595
1109	12	0.2372017929231376
1109	13	0.28313705452964655
1109	14	0.4836275535766319
1109	15	0.3449736418225413
1109	16	0.2868894111179259
1109	17	0.3190212407927504
1109	18	0.40644894431842005
1109	19	0.3751742755515437
1109	20	0.3412273638353511
1109	21	0.40523606989634686
1109	22	0.3778858898546389
1109	23	0.4062047725522362
1109	24	0.39236153497369486
1109	25	0.2704734037319909
1110	1	-0.08077278507471775
1110	2	-0.0533611617484091
1110	3	-0.0786476383535053
1110	4	-0.0366369231454442
1110	5	0.03453243374114123
1110	6	-0.028696718301716828
1110	7	-0.020994184767168633
1110	8	-0.07662994021819003
1110	9	-0.014306534735453624
1110	10	-0.04739579642043168
1110	11	-0.028942337352830805
1110	12	0.008172682308568824
1110	13	-0.07123150109740806
1110	14	0.21737630879262326
1110	15	0.04935614241360753
1110	16	-0.03637932227910312
1110	17	-0.13360749140325368
1110	18	0.05395819371264565
1110	19	0.06513236997560767
1110	20	-0.11206558543799616
1110	21	-0.0921968816805645
1110	22	-0.07043849577477346
1110	23	0.029037914993764957
1110	24	-0.03389567383867842
1110	25	-0.03957061631915337
1111	1	-0.32254841305723403
1111	2	-0.36802122467354437
1111	3	-0.4335845685113071
1111	4	-0.29878239253162064
1111	5	-0.3480314627571772
1111	6	-0.411515161967948
1111	7	-0.41323112531322437
1111	8	-0.3687214032687933
1111	9	-0.6094903278294055
1111	10	-0.43554723115290983
1111	11	-0.46344716649262097
1111	12	-0.44309364301378906
1111	13	-0.5524530478734206
1111	14	-0.39140435211002966
1111	15	-0.5188782836996921
1111	16	-0.4826516239931351
1111	17	-0.5068908884153203
1111	18	-0.3450374033735528
1111	19	-0.4453145360515194
1111	20	-0.4105926205535084
1111	21	-0.4035889649924016
1111	22	-0.4892980556735367
1111	23	-0.45313999426602436
1111	24	-0.5992325182060014
1111	25	-0.6544549844302097
1112	1	-0.6861921661243713
1112	2	-0.6951870827542521
1112	3	-0.7315882531097806
1112	4	-0.6027434746706248
1112	5	-0.6342653148834216
1112	6	-0.7733554998228129
1112	7	-0.7700809059594746
1112	8	-0.7747498811257145
1112	9	-0.9388803997674984
1112	10	-0.9160271726391898
1112	11	-0.850896904395519
1112	12	-0.8210957785953283
1112	13	-0.8032498333725044
1112	14	-0.7751861976579733
1112	15	-0.8561017554299326
1112	16	-1.0761715521885336
1112	17	-1.0486682584674085
1112	18	-0.9780121410484597
1112	19	-0.9784884122774002
1112	20	-0.9383534491665486
1112	21	-0.9562723394544292
1112	22	-0.9459647694803692
1112	23	-1.0280619387475127
1112	24	-0.8301842291890438
1112	25	-0.8621370510831383
1113	1	-1.0655550626017174
1113	2	-1.225633341140366
1113	3	-1.0837345473073832
1113	4	-1.0243366961454399
1113	5	-1.1334324228457007
1113	6	-1.1704880187325628
1113	7	-1.289885158275574
1113	8	-1.2224140078129864
1113	9	-1.2448327343291083
1113	10	-1.311418446370297
1113	11	-1.25925983203215
1113	12	-1.2020378715515179
1113	13	-1.2705987939679062
1113	14	-1.269184819759154
1113	15	-1.419917464869826
1113	16	-1.3451162509898893
1113	17	-1.4314868243282461
1113	18	-1.4173820538517106
1113	19	-1.388386081458222
1113	20	-1.5257367048811048
1113	21	-1.4830834499522183
1113	22	-1.3950778156996435
1113	23	-1.7313542655935663
1113	24	-1.769822568645651
1113	25	-1.7338000185735396
1114	1	-2.5192401740773427
1114	2	-2.6658344669081053
1114	3	-2.273653724288671
1114	4	-5.410161244321678
1114	5	-3.150734406750953
1114	6	-2.6044251353522796
1114	7	-2.3879738281610514
1114	8	-2.584320075201326
1114	9	-3.201440234594642
1114	10	-2.9323868396773562
1114	11	-2.7058719539317835
1114	12	-2.85977750029371
1114	13	-2.7678792462120754
1114	14	-2.758531186607499
1114	15	-3.390730637362566
1114	16	-3.3526277425341604
1114	17	-3.220256617293643
1114	18	-3.0646595364971247
1114	19	-3.1504777368505756
1114	20	-3.3644709567551203
1114	21	-3.4009096464502306
1114	22	-3.545776660020131
1114	23	-3.6220842471669954
1114	24	-3.6192733208423817
1114	25	-3.521287878284223
1115	1	-2.62294002078713
1115	2	-2.0840851664014544
1115	3	-1.614466666785333
1115	4	-1.4965722783794675
1115	5	-1.513912722609291
1115	6	-1.5291284655737911
1115	7	-1.597444927999021
1115	8	-1.5550900613715726
1115	9	-1.8262064611712958
1115	10	-1.73096568341429
1115	11	-1.7167844451358363
1115	12	-2.136870043011747
1115	13	-2.17668382027108
1115	14	-2.403953443575652
1115	15	-2.2406330125496012
1115	16	-2.097812906178767
1115	17	-2.087584626024423
1115	18	-1.9481616914827669
1115	19	-1.948213593843036
1115	20	-1.9711552924937124
1115	21	-1.947576525764923
1115	22	-2.014595068107667
1115	23	-2.11594651328069
1115	24	-2.1298572934886693
1115	25	-2.2302802810897617
1116	1	-1.4026720080308266
1116	2	-1.3517191765885919
1116	3	-1.4138497514919095
1116	4	-1.2880116342968009
1116	5	-1.3874823066819175
1116	6	-1.4674541310778224
1116	7	-1.592643382039654
1116	8	-1.6067608647571134
1116	9	-1.8687068975116858
1116	10	-2.303017833182416
1116	11	-1.8937314331828077
1116	12	-2.0148189440397886
1116	13	-1.955860904914637
1116	14	-2.7991039261716253
1116	15	-2.6975346381252194
1116	16	-2.5933880627842782
1116	17	-2.2761062849648113
1116	18	-2.2003646693157606
1116	19	-2.1273804341308917
1116	20	-2.2751571765264984
1116	21	-2.4393379815410725
1116	22	-2.283097043181236
1116	23	-2.61096103567062
1116	24	-2.683408755982903
1116	25	-2.570809992151773
1201	1	1.0222719970550251
1201	2	1.0071827701449116
1201	3	0.9987706631225812
1201	4	1.0910166372962067
1201	5	1.0746111050792138
1201	6	1.005505372731342
1201	7	1.064383964468739
1201	8	1.1089178906087511
1201	9	1.276929824105828
1201	10	1.2245230403657956
1201	11	1.224701310224873
1201	12	1.299406567343695
1201	13	1.2853619559649516
1201	14	1.7288917688979808
1201	15	1.5776624004763349
1201	16	1.622982840145852
1201	17	1.5478263997841282
1201	18	1.6685978128583585
1201	19	1.5518322200629555
1201	20	1.5470515626205952
1201	21	1.5321105870413803
1201	22	1.5491774533482854
1201	23	1.573253758237918
1201	24	1.5956015175308806
1201	25	1.5848461301008712
1202	1	0.8859060465718862
1202	2	0.8640277478026412
1202	3	0.8041409847714043
1202	4	0.9839721579698448
1202	5	0.8646670039037545
1202	6	0.8268254539582003
1202	7	0.8263367602032616
1202	8	0.8612517157875743
1202	9	1.043399892055831
1202	10	1.0189275263593558
1202	11	1.091256182729488
1202	12	1.1128048152160375
1202	13	1.1610625219621298
1202	14	1.4897039573210122
1202	15	1.3715857635906445
1202	16	1.361383454839391
1202	17	1.2670440812548023
1202	18	1.357260922406347
1202	19	1.237342444817692
1202	20	1.338321993779573
1202	21	1.3053080936061505
1202	22	1.287258307447317
1202	23	1.3723471654683834
1202	24	1.3801078090908234
1202	25	1.4335225670751726
1203	1	0.7883874727938434
1203	2	0.8006930105992751
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2211	9	-0.14026243665748694
2211	10	-0.01421098285547373
2211	11	-0.002782891231220219
2211	12	0.08100434373584875
2211	13	-0.030057109323969655
2211	14	0.2499877801033108
2211	15	0.07168440985493163
2211	16	0.0423009947581221
2211	17	-0.14166354035651807
2211	18	-0.009009876461981037
2211	19	-0.06198202206986991
2211	20	-0.02385634066627773
2211	21	-0.052653974558161565
2211	22	-0.07699574492277605
2211	23	-0.05824561558316077
2211	24	-0.07069480207101009
2211	25	-0.14100625608467254
2212	1	-0.3188167553748116
2212	2	-0.2241774150393141
2212	3	-0.35399982327985313
2212	4	-0.2695026307753139
2212	5	-0.23811419150489854
2212	6	-0.2896089967113227
2212	7	-0.42346473813891095
2212	8	-0.39385724544987255
2212	9	-0.4596409490196436
2212	10	-0.5698294768612655
2212	11	-0.5821382821679464
2212	12	-0.519637249011133
2212	13	-0.44225347563894957
2212	14	-0.4893282804886212
2212	15	-0.5176865259716337
2212	16	-0.5440744667802022
2212	17	-0.6579887817408689
2212	18	-0.432993302945043
2212	19	-0.6286260182462655
2212	20	-0.6352537818567043
2212	21	-0.6736467935532033
2212	22	-0.8595182679654982
2212	23	-0.840806110665973
2212	24	-0.7255236508679133
2212	25	-0.8190876864510059
2213	1	-0.6995573381213163
2213	2	-0.503864600514035
2213	3	-0.5368221538941285
2213	4	-0.4250639221661032
2213	5	-0.5593360421721343
2213	6	-0.5315457796925571
2213	7	-0.5859065886558668
2213	8	-0.4772222584339332
2213	9	-0.5084488524850101
2213	10	-0.6753052206717385
2213	11	-0.6835385099872298
2213	12	-0.5908293402727836
2213	13	-0.75569967808174
2213	14	-0.5485439214258108
2213	15	-0.7074304395305475
2213	16	-0.7471762471110992
2213	17	-0.8050099476319943
2213	18	-0.7211033954766961
2213	19	-0.7784194820393893
2213	20	-0.8547955674236718
2213	21	-0.7632280487264964
2213	22	-0.9305952236132384
2213	23	-0.8654788527773576
2213	24	-0.9661413177120579
2213	25	-0.9333592427736619
2214	1	-1.009780454709965
2214	2	-0.96093308958298
2214	3	-0.9603088616420088
2214	4	-0.8112409804204077
2214	5	-0.8037137832266197
2214	6	-0.9422659428470155
2214	7	-0.9144315554790907
2214	8	-1.0321929625968238
2214	9	-1.0627396791025774
2214	10	-1.0904707603042851
2214	11	-1.1319111638475121
2214	12	-1.0151116193319538
2214	13	-1.2338112580055451
2214	14	-1.0018436305433127
2214	15	-1.2340599820598825
2214	16	-1.1967236823249294
2214	17	-1.2996276257959138
2214	18	-1.078596703271252
2214	19	-1.2310840111812809
2214	20	-1.222236368571707
2214	21	-1.2058615447318886
2214	22	-1.2726308448252865
2214	23	-1.2347741109154171
2214	24	-1.2810282761449088
2214	25	-1.224849862808206
2215	1	-1.4813183758655235
2215	2	-1.6050357959004415
2215	3	-1.447795964360104
2215	4	-1.5457511875154637
2215	5	-1.4876033309827434
2215	6	-1.2987644061931576
2215	7	-1.4092921045900084
2215	8	-1.4891459541874212
2215	9	-1.8428533253177832
2215	10	-1.5113774065540184
2215	11	-1.6858237863075516
2215	12	-1.7490767928921755
2215	13	-1.8041272566404842
2215	14	-2.4035572054623486
2215	15	-2.132347458587475
2215	16	-1.9386061984306409
2215	17	-1.8311180225749695
2215	18	-1.781419215693866
2215	19	-1.9866980158235208
2215	20	-2.2243190669719795
2215	21	-2.012943937555484
2215	22	-2.3180797263464115
2215	23	-1.9966614353674288
2215	24	-1.9598059734858992
2215	25	-1.9592391029909138
2216	1	-1.9440943882319637
2216	2	-1.7551790111624044
2216	3	-1.8615685904610935
2216	4	-1.6906874287154778
2216	5	-2.246412380969822
2216	6	-1.960612870652266
2216	7	-2.064810991972884
2216	8	-2.4658319471973016
2216	9	-2.472687690375203
2216	10	-2.502801652059233
2216	11	-2.3748355524305254
2216	12	-2.5269895219216423
2216	13	-2.4810931394817928
2216	14	-2.476719707996054
2216	15	-2.508211590919281
2216	16	-2.559109715186146
2216	17	-2.751250338841409
2216	18	-2.9606432564300746
2216	19	-2.770443617855406
2216	20	-2.9062817798439937
2216	21	-2.916062682208789
2216	22	-3.129494076625747
2216	23	-2.843980301897794
2216	24	-2.9750244164254767
2216	25	-3.0239961680721024
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	131791.0
3	17.0
4	921.0
5	5607.0
6	7658.0
7	7021.0
8	6497.0
9	5884.0
10	5609.0
11	5248.0
12	5045.0
13	4874.0
14	4971.0
15	5054.0
16	5101.0
17	5225.0
18	6180.0
19	7454.0
20	9672.0
21	12222.0
22	15346.0
23	18453.0
24	21457.0
25	24739.0
26	27380.0
27	31328.0
28	35476.0
29	42570.0
30	52903.0
31	67546.0
32	87595.0
33	115385.0
34	156984.0
35	217036.0
36	320629.0
37	565329.0
38	1552271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97974360790424	17.93768713962812	12.516193904120934	25.566375348346703
2	16.211931226013192	20.742774066331133	38.371078456354304	24.67421625130137
3	18.347870401223073	24.97226523452047	29.175573245821585	27.50429111843487
4	12.467387289604789	16.76208605975172	38.296709928125324	32.473816722518166
5	14.912996364917575	35.92124650922578	34.045785962291376	15.119971163565266
6	31.707334836622366	37.36794632978053	17.13460972842757	13.79010910516954
7	28.16690465800113	31.292368573043543	22.208316830795724	18.3324099381596
8	26.107372418334375	33.468027104565664	20.569915142083865	19.854685335016097
9	26.87976689560182	15.255242202336925	19.408170472714726	38.456820429346536
10	15.510110229763436	27.089556711558078	32.41703920581245	24.983293852866034
11	35.07384617711487	22.640559254096335	23.451101257671915	18.83449331111688
12	24.114847196796724	24.448036379687785	29.495673246051364	21.94144317746413
13	28.82369663335168	21.04498993828535	25.274607088992614	24.856706339370362
14	22.901502833320546	20.675846509473434	26.641698793689343	29.78095186351668
15	24.222864898504014	27.642455396828332	23.396282746942575	24.738396957725083
16	24.343823621761413	27.16325261029452	24.425415895115975	24.06750787282809
17	23.849895075788556	26.19318379159015	25.641673454342563	24.31524767827873
18	24.641542176665357	25.427160955782966	26.586207727127853	23.345089140423823
19	25.694937299500964	25.580554471041033	25.79164736479776	22.932860864660242
20	24.894543959343547	25.591394114852694	25.74707131909401	23.766990606709747
21	25.167748673737268	25.446431197206117	25.655317052485582	23.730503076571033
22	24.3472405872489	25.403273579037084	26.028843727339364	24.220642106374655
23	23.787809867818112	25.032742970204737	26.14600395716581	25.03344320481134
24	23.8908344084889	25.950343218788884	26.049138017482225	24.10968435523999
25	24.164698224845445	25.45177070140759	25.996483411594884	24.387047662152085
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13487.0
1	13487.0
2	12525.5
3	11564.0
4	11564.0
5	11564.0
6	8830.5
7	6097.0
8	6097.0
9	6097.0
10	8355.0
11	10613.0
12	10613.0
13	10613.0
14	15035.5
15	19458.0
16	19458.0
17	19458.0
18	32262.5
19	45067.0
20	45067.0
21	45067.0
22	64110.5
23	83154.0
24	83154.0
25	83154.0
26	111962.5
27	140771.0
28	140771.0
29	140771.0
30	168955.5
31	197140.0
32	197140.0
33	197140.0
34	232148.5
35	267157.0
36	267157.0
37	267157.0
38	295511.5
39	323866.0
40	323866.0
41	323866.0
42	354094.5
43	384323.0
44	384323.0
45	384323.0
46	406078.5
47	427834.0
48	427834.0
49	427834.0
50	431440.5
51	435047.0
52	435047.0
53	435047.0
54	405896.5
55	376746.0
56	376746.0
57	376746.0
58	340227.5
59	303709.0
60	303709.0
61	303709.0
62	263240.5
63	222772.0
64	222772.0
65	222772.0
66	182775.0
67	142778.0
68	142778.0
69	142778.0
70	111665.5
71	80553.0
72	80553.0
73	80553.0
74	63561.5
75	46570.0
76	46570.0
77	46570.0
78	38216.0
79	29862.0
80	29862.0
81	29862.0
82	22423.5
83	14985.0
84	14985.0
85	14985.0
86	11235.5
87	7486.0
88	7486.0
89	7486.0
90	5298.5
91	3111.0
92	3111.0
93	3111.0
94	1694.0
95	277.0
96	277.0
97	277.0
98	164.0
99	51.0
100	51.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2295743637880104
2	0.6466585690606536
3	0.6439878057398043
4	0.6510263799082927
5	0.6675517279560481
6	0.682380028477014
7	0.6765933746151735
8	0.6720586410599815
9	0.6646027601226103
10	0.6659103213317762
11	0.6718917183524284
12	0.671418770681028
13	0.6680524960787074
14	0.6609026401051835
15	0.6674682666022715
16	0.6756752997236316
17	0.6755361974673374
18	0.6689149300677316
19	0.6680246756274486
20	0.6752023520522312
21	0.670667618497039
22	0.6714465911322868
23	0.6745346612220189
24	0.6802656741813415
25	0.6669118575770947
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3594478.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.204538411417495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.41275640052174	53.50057406763613
2	7.601978996728585	9.305512310161273
3	1.8980824824418252	3.485137866139481
4	0.8191805572280142	2.005502715229935
5	0.47420272174539196	1.4511679348932283
6	0.3247378809559221	1.1925259265165433
7	0.21178259398684135	0.9073439135976087
8	0.16160184877243286	0.7912613248438758
9	0.1306217966480308	0.719518209328149
>10	0.827367810230638	9.504666436713787
>50	0.07257833318408453	3.0649359600075523
>100	0.054138998641809766	6.818137911085207
>500	0.007160059885588122	2.9525903193511143
>1k	0.0036259277506378207	3.4902063697776193
>5k	1.835912785133074E-4	0.8109187347185421
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9375	0.26081673055169624	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	8055	0.2240937348900174	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	6119	0.17023334125288847	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5318	0.14794915979455153	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.78204512588476E-5	0.0	0.0	0.0	0.0
3	2.78204512588476E-5	0.0	0.0	0.0	0.0
4	2.78204512588476E-5	0.0	0.0	5.56409025176952E-5	0.0
5	2.78204512588476E-5	0.0	0.0	1.112818050353904E-4	0.0
6	2.78204512588476E-5	0.0	0.0	1.112818050353904E-4	2.78204512588476E-5
7	2.78204512588476E-5	0.0	0.0	1.112818050353904E-4	2.78204512588476E-5
8	2.78204512588476E-5	0.0	0.0	1.112818050353904E-4	2.78204512588476E-5
9	2.78204512588476E-5	0.0	0.0	1.112818050353904E-4	2.78204512588476E-5
10	5.56409025176952E-5	0.0	0.0	1.39102256294238E-4	2.78204512588476E-5
11	5.56409025176952E-5	0.0	0.0	1.669227075530856E-4	2.78204512588476E-5
12	5.56409025176952E-5	0.0	0.0	1.669227075530856E-4	1.39102256294238E-4
13	5.56409025176952E-5	0.0	0.0	1.669227075530856E-4	1.669227075530856E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1530	0.0	15.711155	1
GTATCAA	5225	0.0	13.292604	1
TAGATCG	70	1.0903662E-4	12.215843	5
CGAACGA	195	0.0	11.692161	16
ACGCTTA	160	4.5474735E-11	11.280477	17
ATACCGT	380	0.0	11.250489	6
CGTTAAG	60	0.0058726096	11.084747	2
TACCGTC	375	0.0	10.892739	7
CGTCGTA	350	0.0	10.85655	10
ACCGTCG	390	0.0	10.717363	8
ATTATAC	475	0.0	10.601352	3
GTATTAG	400	0.0	10.451332	1
CGCTTAC	175	2.3646862E-10	10.313723	18
CCGTCGT	370	0.0	10.269423	9
GCGTTAT	215	1.8189894E-12	10.164087	1
TATCACG	75	0.0026462085	10.134626	2
CGGTTCT	490	0.0	10.081083	12
CTTACAC	445	0.0	10.034987	3
CGACCAT	370	0.0	10.012967	10
CGTTATT	200	2.5465852E-11	9.976272	2
>>END_MODULE