FastQCFastQC Report
Thu 2 Feb 2017
SRR4050265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594478
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT152180.4233716272571427No Hit
TATCAACGCAGAGTACTTTTTTTTT100220.27881656251617065No Hit
GGTATCAACGCAGAGTACTTTTTTT55460.15429222268156878No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45910.12772369172936932No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45230.12583190104376768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.015.8841571
GTATCAA61550.013.37830451
GTCGTAT500.001464417413.3426911
GTATACG603.9851357E-412.7073251
AACCGCG3200.012.1686397
ACGCTTA1103.84116E-812.08601417
CGTCGTA4100.011.815083510
CGACCAT3750.011.3980810
CGGTATA600.005872179811.084862
ATACCGT4650.011.0288656
TCGGCGT2500.011.01814413
GCGTTAT1752.0008883E-1110.8919931
AGAACCG4300.010.82737355
CGGTTCT4650.010.82613412
CCGACCA4000.010.68579
CACGCAA803.7791487E-410.68569911
CGAGCCG5400.010.55333615
GTCGTAG4550.010.43780211
GAACCGC3850.010.3604496
TACCGTC4500.010.3416957
Produced by FastQC (version 0.11.5)