##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3594478
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.722758353229594	31.0	31.0	34.0	27.0	34.0
2	30.72213656614396	31.0	31.0	34.0	27.0	34.0
3	30.90845875256435	31.0	31.0	34.0	27.0	34.0
4	34.553523766176895	37.0	35.0	37.0	31.0	37.0
5	34.4977523857428	37.0	35.0	37.0	32.0	37.0
6	34.46946149065316	37.0	35.0	37.0	31.0	37.0
7	34.41781059725501	37.0	35.0	37.0	31.0	37.0
8	34.391778444603084	37.0	35.0	37.0	31.0	37.0
9	35.98037795752262	39.0	37.0	39.0	31.0	39.0
10	35.866166380765165	39.0	37.0	39.0	30.0	39.0
11	35.85460920890321	39.0	37.0	39.0	30.0	39.0
12	35.78032776942855	39.0	37.0	39.0	30.0	39.0
13	35.779616678694374	39.0	37.0	39.0	30.0	39.0
14	36.941328615726675	40.0	37.0	41.0	31.0	41.0
15	36.91268467911057	40.0	37.0	41.0	31.0	41.0
16	36.89754006005879	40.0	37.0	41.0	31.0	41.0
17	36.876009812829565	40.0	37.0	41.0	31.0	41.0
18	36.84108986061398	40.0	37.0	41.0	31.0	41.0
19	36.89549358766419	40.0	37.0	41.0	31.0	41.0
20	36.84988195782531	40.0	37.0	41.0	31.0	41.0
21	36.797003069708595	40.0	37.0	41.0	30.0	41.0
22	36.73853032345726	40.0	37.0	41.0	30.0	41.0
23	36.64144167804059	40.0	37.0	41.0	30.0	41.0
24	36.58616271959378	40.0	37.0	41.0	30.0	41.0
25	36.36601364648775	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.846224449717365
1101	2	1.023388245457486
1101	3	0.9545158362585155
1101	4	0.8507519749856485
1101	5	0.8822831406934739
1101	6	0.9708634028344676
1101	7	0.9852461156727941
1101	8	0.9801069728275991
1101	9	1.1372114395881496
1101	10	1.1719464205458578
1101	11	1.1100868329522697
1101	12	1.1612876427603567
1101	13	1.1581724723137796
1101	14	1.230152586706815
1101	15	1.3665361086533778
1101	16	1.3104236014484556
1101	17	1.3543798986235274
1101	18	1.4459228170695582
1101	19	1.3143025308749472
1101	20	1.3350244581892596
1101	21	1.340008269245324
1101	22	1.4039015257455816
1101	23	1.3919657361144644
1101	24	1.4184945073648763
1101	25	1.4529139418905999
1102	1	0.6119015651844926
1102	2	0.8253595183028182
1102	3	0.7132417869142316
1102	4	0.6549853798985055
1102	5	0.7040589217610531
1102	6	0.8141079942657328
1102	7	0.7397128760853136
1102	8	0.7644299095574993
1102	9	0.8614771753382797
1102	10	0.9590632900327591
1102	11	0.9613737680021188
1102	12	0.952393582886188
1102	13	0.8796688079086366
1102	14	1.0919998498602013
1102	15	1.0646637491703785
1102	16	1.114768794989807
1102	17	1.0467102765613063
1102	18	1.1127075360077185
1102	19	1.1211505717640122
1102	20	1.0857299879768831
1102	21	1.1277758616436273
1102	22	1.1855715486809544
1102	23	1.2567651760055867
1102	24	1.1988635194526651
1102	25	1.2373472753283252
1103	1	0.5958999685166155
1103	2	0.8025773514853292
1103	3	0.6387539797212725
1103	4	0.5586759550085034
1103	5	0.6446619048378253
1103	6	0.7015695365661969
1103	7	0.6229330140956435
1103	8	0.6495744538222752
1103	9	0.8089082659446021
1103	10	0.758826085041207
1103	11	0.7772635374760455
1103	12	0.8378120796807238
1103	13	0.7712450289924107
1103	14	0.8918726261093539
1103	15	0.8571520590180413
1103	16	0.9333342981913475
1103	17	0.9534010157651096
1103	18	0.954885803306162
1103	19	0.9074308428446969
1103	20	0.9240186439326123
1103	21	0.9722381693612547
1103	22	0.8684408657280827
1103	23	0.9517310001416206
1103	24	0.9827890974790279
1103	25	0.9494047886969881
1104	1	0.46779196838388515
1104	2	0.5576073604285057
1104	3	0.5455775892829529
1104	4	0.48792224258313155
1104	5	0.5090329238246198
1104	6	0.5603369237691567
1104	7	0.5402072076483861
1104	8	0.5319551266157987
1104	9	0.6368185165153335
1104	10	0.5494419757836013
1104	11	0.57675778131469
1104	12	0.6149344050001773
1104	13	0.7455857186022428
1104	14	0.810447753810621
1104	15	0.8191987528849438
1104	16	0.7966539312257055
1104	17	0.6287765539306704
1104	18	0.7454183696740984
1104	19	0.7717125498455246
1104	20	0.7429164189091324
1104	21	0.6544407821772253
1104	22	0.6775025015443177
1104	23	0.7234864940552512
1104	24	0.7913687112533552
1104	25	0.7798372530167157
1105	1	0.4066014433442362
1105	2	0.5712166902032401
1105	3	0.44925142344222024
1105	4	0.41471471497209933
1105	5	0.39189566682592414
1105	6	0.5373964956054422
1105	7	0.45666565272708226
1105	8	0.48030131266384046
1105	9	0.5391761319523596
1105	10	0.5395432673659784
1105	11	0.583912768597564
1105	12	0.5851768190256195
1105	13	0.5289812178629703
1105	14	0.6409168489578434
1105	15	0.6658116796549223
1105	16	0.6276521165542093
1105	17	0.6274918277863364
1105	18	0.6845358175066636
1105	19	0.5890185654186979
1105	20	0.6762833666343511
1105	21	0.6508101006577647
1105	22	0.6655587089916963
1105	23	0.6942756222075843
1105	24	0.6820518756186615
1105	25	0.7519048980329757
1106	1	0.3293921150156578
1106	2	0.49259093470953985
1106	3	0.4257742776050968
1106	4	0.4244708880940635
1106	5	0.5357629969157358
1106	6	0.5338357892186067
1106	7	0.5050527517986438
1106	8	0.45269802194641073
1106	9	0.5024804948272035
1106	10	0.5597641654798622
1106	11	0.5105565694096654
1106	12	0.6063195325107884
1106	13	0.5607413842006608
1106	14	0.7474554459482192
1106	15	0.6572441060337866
1106	16	0.6315145753054026
1106	17	0.654125978505121
1106	18	0.6787278856997077
1106	19	0.6276768392342191
1106	20	0.6876326953831224
1106	21	0.7545979581340774
1106	22	0.6642008945704632
1106	23	0.7285544272910158
1106	24	0.7207603227975738
1106	25	0.694802447158537
1107	1	0.27024165983841897
1107	2	0.4665463820488682
1107	3	0.3791143499693419
1107	4	0.42618586358187116
1107	5	0.41873271293651015
1107	6	0.4159556780934608
1107	7	0.47766940204810027
1107	8	0.43057345752456655
1107	9	0.4125459352702734
1107	10	0.42841052576622474
1107	11	0.44715074673546695
1107	12	0.4379362147798318
1107	13	0.45781241911818427
1107	14	0.5620115117129956
1107	15	0.5597661814670971
1107	16	0.5629679577262436
1107	17	0.5398764399485572
1107	18	0.5688057759056093
1107	19	0.5405066021550624
1107	20	0.618414492976008
1107	21	0.5449125522101781
1107	22	0.5642483730631227
1107	23	0.4985252963608957
1107	24	0.5259132713703494
1107	25	0.5623611058205356
1108	1	0.10399077385210731
1108	2	0.37824408028578915
1108	3	0.24146642110214955
1108	4	0.24716390801295063
1108	5	0.28463473377049553
1108	6	0.3253066920471426
1108	7	0.3731306711671607
1108	8	0.36932997944615664
1108	9	0.414544493410439
1108	10	0.4009969116586447
1108	11	0.4132249435280855
1108	12	0.41497427362423167
1108	13	0.34595571512319623
1108	14	0.4344649155302136
1108	15	0.47067861639304454
1108	16	0.40658924557390463
1108	17	0.41110896089518434
1108	18	0.4184944616607922
1108	19	0.4396988782415292
1108	20	0.429953673728825
1108	21	0.4462914378977203
1108	22	0.4728128040464554
1108	23	0.42445255172145124
1108	24	0.4200542212849214
1108	25	0.44427389401929673
1109	1	0.11126141820146529
1109	2	0.28282681086164985
1109	3	0.29936945715391516
1109	4	0.2145161528932178
1109	5	0.31089138722959575
1109	6	0.34792605012101063
1109	7	0.37614799652863695
1109	8	0.3803379800511024
1109	9	0.34171265556913966
1109	10	0.3728910734824069
1109	11	0.3611269337782659
1109	12	0.3844736580143646
1109	13	0.40105437342276673
1109	14	0.43229164689357447
1109	15	0.5178983722788928
1109	16	0.4486098462739392
1109	17	0.43107718329408584
1109	18	0.3732901812197085
1109	19	0.3850501755988205
1109	20	0.3692581628401115
1109	21	0.33570810402864737
1109	22	0.35622263535795895
1109	23	0.4016231422021619
1109	24	0.38770874929073074
1109	25	0.36664266708397975
1110	1	-0.08566781402866042
1110	2	0.05791627506299335
1110	3	-0.007525263518019187
1110	4	0.005545467645269753
1110	5	0.0320235219635947
1110	6	-0.10309048534699627
1110	7	-0.03669196086649862
1110	8	0.07503144171012366
1110	9	0.07587986542938552
1110	10	0.04220923165004109
1110	11	0.1255953285813547
1110	12	0.07151462243738393
1110	13	0.06608766840254532
1110	14	0.025034115502904797
1110	15	-0.037642955879171325
1110	16	0.06767765208693532
1110	17	0.03105982647328176
1110	18	0.02623471935050503
1110	19	0.04287301948883737
1110	20	0.04044001945869269
1110	21	-0.006217912839851181
1110	22	-0.01843651501388166
1110	23	-0.0320290207655205
1110	24	-0.08253495092489516
1110	25	0.12039586813514092
1111	1	-0.3778663753603908
1111	2	-0.24023395865873098
1111	3	-0.32945738362077037
1111	4	-0.2521585269668236
1111	5	-0.2823206989027014
1111	6	-0.42664870969105095
1111	7	-0.37909380620064326
1111	8	-0.35289579129952386
1111	9	-0.36472704906494613
1111	10	-0.3963537554915888
1111	11	-0.41064394743426647
1111	12	-0.34632744786466674
1111	13	-0.3138606252184388
1111	14	-0.2976115923377378
1111	15	-0.3253403134737596
1111	16	-0.276291351489391
1111	17	-0.3547329294045696
1111	18	-0.3684878520596442
1111	19	-0.40081183808652554
1111	20	-0.45721504550004965
1111	21	-0.37706964757334305
1111	22	-0.41419243104044057
1111	23	-0.42817826431749495
1111	24	-0.44768145116698577
1111	25	-0.34860106698049975
1112	1	-0.46683236558334684
1112	2	-0.35670911511010317
1112	3	-0.3902640963094548
1112	4	-0.3772447372929193
1112	5	-0.4441017723212113
1112	6	-1.4588099227034803
1112	7	-0.6426476977818751
1112	8	-0.4633424434602347
1112	9	-0.5884304974383383
1112	10	-0.587075672719898
1112	11	-0.5328206757281038
1112	12	-0.5531880068668116
1112	13	-0.4842666830906097
1112	14	-0.5787675766249976
1112	15	-0.5642581480379647
1112	16	-0.458207847581356
1112	17	-0.559358066645224
1112	18	-0.6021767764167336
1112	19	-0.6299994408228926
1112	20	-0.652107836213986
1112	21	-0.6491163813614378
1112	22	-0.7232033876918891
1112	23	-0.6004620162004031
1112	24	-0.6557148750402746
1112	25	-0.628969828172707
1113	1	-0.7607554040878277
1113	2	-0.8684695251632348
1113	3	-0.8002549626322661
1113	4	-0.7048098514590393
1113	5	-0.7253022270477274
1113	6	-1.24017940570765
1113	7	-0.9636280714896586
1113	8	-0.8058366832669464
1113	9	-0.9127592821539849
1113	10	-0.9349531703337632
1113	11	-0.9639673443220005
1113	12	-1.0392891409244456
1113	13	-0.9615348359964955
1113	14	-1.1848807528149834
1113	15	-1.0684097208890222
1113	16	-1.0728246505105687
1113	17	-1.1742436917797292
1113	18	-1.121510704711234
1113	19	-1.2033317217328587
1113	20	-1.2045520142010275
1113	21	-1.1688385630289488
1113	22	-1.1977928373109634
1113	23	-1.1010651563571443
1113	24	-1.1816837150913386
1113	25	-1.1938352559145429
1114	1	-0.8636400198825953
1114	2	-0.8913249723972321
1114	3	-0.993749852676693
1114	4	-0.8553739535290248
1114	5	-1.1003715835511088
1114	6	-1.5101452401414406
1114	7	-1.2123006909535334
1114	8	-1.4219773395229325
1114	9	-1.501505132650962
1114	10	-1.693444849021219
1114	11	-1.4692926365790697
1114	12	-1.4487941503377115
1114	13	-1.5789182574153813
1114	14	-1.6936253366846188
1114	15	-1.6199954903910978
1114	16	-1.5191565235841722
1114	17	-1.6727023985115679
1114	18	-1.6832656500090124
1114	19	-1.6206088749246845
1114	20	-1.532488983131195
1114	21	-1.5984150741576428
1114	22	-1.4873199093249738
1114	23	-1.5740394113786564
1114	24	-1.6661405441133823
1114	25	-1.6057485133199876
1115	1	-1.2642655773761575
1115	2	-1.9246470352175642
1115	3	-1.470244695127775
1115	4	-1.1618778562449705
1115	5	-1.2757633638480002
1115	6	-1.8315910206432449
1115	7	-1.4442570553776761
1115	8	-1.471927681261974
1115	9	-1.4818523296879604
1115	10	-1.4762360862757689
1115	11	-1.4854660784792841
1115	12	-1.427385189975496
1115	13	-1.5314135859717979
1115	14	-1.7701466024974764
1115	15	-1.6303722695689444
1115	16	-1.7643269335143046
1115	17	-1.6753406343386104
1115	18	-1.8999771886790526
1115	19	-1.8840451380354608
1115	20	-1.7366601160562283
1115	21	-2.019105809109213
1115	22	-2.129374873087059
1115	23	-2.0398128942924316
1115	24	-1.957542403826011
1115	25	-2.002102788718787
1116	1	-1.0016540100910696
1116	2	-1.087361938450364
1116	3	-1.124797185467525
1116	4	-0.9985350146372483
1116	5	-0.9673457900766067
1116	6	-1.2169343706476852
1116	7	-1.2467192267762428
1116	8	-1.2282399008798706
1116	9	-1.386861178745967
1116	10	-1.5208156479784094
1116	11	-1.5404693930217022
1116	12	-1.3542218444252754
1116	13	-1.4471210635703144
1116	14	-1.627339389300559
1116	15	-1.5654254486819994
1116	16	-1.5991654043783115
1116	17	-1.688434860476086
1116	18	-1.6892282525164575
1116	19	-1.7152026542183876
1116	20	-1.6432433213734043
1116	21	-1.7464131767090763
1116	22	-1.7259533308370365
1116	23	-1.6452102283754044
1116	24	-1.6446639887294126
1116	25	-1.7869213307317295
1201	1	0.7057414961300594
1201	2	0.9175381010890256
1201	3	0.8662006994217855
1201	4	0.7211507961807087
1201	5	0.8508612116695744
1201	6	0.931255624227127
1201	7	0.9765932386524199
1201	8	0.9899800500005611
1201	9	1.1294920849132097
1201	10	1.1818933416617057
1201	11	1.145077412524266
1201	12	1.189528513181628
1201	13	1.2024225675476146
1201	14	1.4188397479077892
1201	15	1.3957686357741608
1201	16	1.3362521180620988
1201	17	1.380008362055328
1201	18	1.40077585519753
1201	19	1.3620437050149405
1201	20	1.318491314152034
1201	21	1.3354652691718343
1201	22	1.4151681410765065
1201	23	1.3275425762398427
1201	24	1.4415080786745165
1201	25	1.4701592539540371
1202	1	0.5966340902201352
1202	2	0.7471544354505113
1202	3	0.6949329846586956
1202	4	0.6318337640123417
1202	5	0.6976584885088428
1202	6	0.7638042887733292
1202	7	0.806483566347751
1202	8	0.811327940286759
1202	9	0.9076604390405691
1202	10	0.9162683552829165
1202	11	0.9212561548608633
1202	12	0.9276632418846447
1202	13	0.9375537434907386
1202	14	1.1081214564684956
1202	15	1.1384195034080946
1202	16	1.1343722953914934
1202	17	1.1557440773086967
1202	18	1.1822698764241224
1202	19	1.1560643562490611
1202	20	1.1867106329573573
1202	21	1.2126037117354969
1202	22	1.1692576855501713
1202	23	1.2474373167708563
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2211	5	-0.21293965093399692
2211	6	-0.1669937655371001
2211	7	-0.26914220854060034
2211	8	-0.24726389833136864
2211	9	-0.29027692699531116
2211	10	-0.36263199437846083
2211	11	-0.3217540670609864
2211	12	-0.3232102181932319
2211	13	-0.31872969488522784
2211	14	-0.395186490376652
2211	15	-0.4240707590261934
2211	16	-0.3974386806989756
2211	17	-0.43942318486190146
2211	18	-0.4812550450530466
2211	19	-0.42832756791749915
2211	20	-0.4548681468700977
2211	21	-0.38339088383319364
2211	22	-0.4510158031917655
2211	23	-0.5443444585831614
2211	24	-0.5055139643912554
2211	25	-0.48127241923713626
2212	1	-0.3391622905568532
2212	2	-0.4037351649089871
2212	3	-0.4056578874215191
2212	4	-0.3283742647747303
2212	5	-0.3807147244756095
2212	6	-0.36908222774233934
2212	7	-0.4329123179459913
2212	8	-0.4711825991211782
2212	9	-0.5427090554106471
2212	10	-0.4784468541493112
2212	11	-0.481049057432557
2212	12	-0.5092609514234212
2212	13	-0.39529367344216126
2212	14	-0.6267779079256997
2212	15	-0.5807577218995164
2212	16	-0.5809744605480276
2212	17	-0.4709055805869298
2212	18	-0.5533145113025455
2212	19	-0.5328457870040708
2212	20	-0.597452305050453
2212	21	-0.5969258951677574
2212	22	-0.553187647157749
2212	23	-0.6147306804242945
2212	24	-0.7926281759565583
2212	25	-0.5980212647125143
2213	1	-0.5429894226188772
2213	2	-0.8265187021771894
2213	3	-0.5971579420628217
2213	4	-0.47934508051991287
2213	5	-0.6188818245596224
2213	6	-0.41116190860023494
2213	7	-0.6134066523220127
2213	8	-0.5150718627581341
2213	9	-0.571997583492653
2213	10	-0.6365807555236813
2213	11	-0.6702685990599377
2213	12	-0.6539122008504847
2213	13	-0.6912694504065229
2213	14	-0.7540326063854721
2213	15	-0.7809748370313088
2213	16	-0.8303972467217875
2213	17	-0.8346940194895822
2213	18	-0.7602852384615915
2213	19	-0.6798263772803992
2213	20	-0.7468657184944831
2213	21	-0.8101933566225696
2213	22	-0.7789082630897823
2213	23	-0.8901596673640313
2213	24	-0.8236759825466535
2213	25	-0.7803513885999678
2214	1	-0.7853707900066276
2214	2	-0.8259452558297582
2214	3	-0.8585395876052395
2214	4	-0.7550146285351076
2214	5	-0.8054032686288153
2214	6	-0.785519551396284
2214	7	-0.7674826832666639
2214	8	-0.8047598154204039
2214	9	-0.9067043175891314
2214	10	-0.9129360869177958
2214	11	-0.9784717943632515
2214	12	-1.0674280695351328
2214	13	-1.0010651007398863
2214	14	-1.2252034184635932
2214	15	-1.3506094736449867
2214	16	-1.3128278839049514
2214	17	-1.2283373133486464
2214	18	-1.3335610991163236
2214	19	-1.2596249535252468
2214	20	-1.2396620274797243
2214	21	-1.3397949468162196
2214	22	-1.15924642052169
2214	23	-1.2486027543472957
2214	24	-1.298175892836035
2214	25	-1.3049846331783783
2215	1	-1.3440511163129791
2215	2	-3.23805566642017
2215	3	-1.7739541186683851
2215	4	-1.3772953942046087
2215	5	-1.2599485485458999
2215	6	-1.1308016825815415
2215	7	-1.2453968952309253
2215	8	-1.3569768032694753
2215	9	-1.522230145288674
2215	10	-1.5164231034981484
2215	11	-1.5176663124440708
2215	12	-1.5501459518973633
2215	13	-1.5969012002287712
2215	14	-1.8622665885175422
2215	15	-1.8179894036216737
2215	16	-1.883929232289205
2215	17	-1.8158421642835876
2215	18	-1.7600534291923111
2215	19	-1.7308947557911054
2215	20	-1.786668642342761
2215	21	-1.7288249413026975
2215	22	-1.7825330111549533
2215	23	-1.9484835415310116
2215	24	-2.040473325956569
2215	25	-2.0249267439645564
2216	1	-1.2734100183573673
2216	2	-2.0220476777090184
2216	3	-1.4396913809935405
2216	4	-1.2324886716679089
2216	5	-1.4194443064858788
2216	6	-1.3645181105345188
2216	7	-1.426376885245034
2216	8	-1.4708379553768367
2216	9	-1.7050292249290493
2216	10	-1.5442317319019807
2216	11	-1.6486070906828587
2216	12	-1.7899115490204949
2216	13	-1.8923429851513802
2216	14	-1.8952439829776395
2216	15	-1.9658786703452904
2216	16	-1.9174982032439942
2216	17	-1.9562371573511044
2216	18	-2.145379223675981
2216	19	-2.0158999268237423
2216	20	-2.136437030593086
2216	21	-2.127247015427521
2216	22	-2.13730211499589
2216	23	-2.2472759742735846
2216	24	-2.127936955496928
2216	25	-2.1800088296553994
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51667.0
3	2.0
4	955.0
5	7910.0
6	9571.0
7	7566.0
8	6254.0
9	5307.0
10	4784.0
11	4354.0
12	4058.0
13	3869.0
14	3751.0
15	3893.0
16	4148.0
17	4357.0
18	4931.0
19	6088.0
20	7885.0
21	10623.0
22	13078.0
23	15942.0
24	19015.0
25	22072.0
26	25262.0
27	28548.0
28	33473.0
29	40973.0
30	52291.0
31	68290.0
32	88693.0
33	117844.0
34	160019.0
35	218774.0
36	318873.0
37	556847.0
38	1662511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.13756621436119	18.733619115511786	13.026218905278226	26.10259576484879
2	16.092256240748778	21.43773719297074	37.28921126729213	25.180795298988347
3	18.218305968210128	25.13716873493175	29.326288824135244	27.318236472722884
4	12.928497545401585	17.47611196952659	37.20075627114702	32.3946342139248
5	14.71369783135414	36.35676402355269	33.91749320515087	15.012044939942301
6	31.737016735672963	37.759956019962395	16.788004978914177	13.715022265450463
7	28.01690092725852	31.514798605258964	22.060706790835305	18.407593676647213
8	26.092324675675144	33.703773048966646	20.31886785996366	19.88503441539455
9	26.795467280852638	15.099297911541418	19.306458802036875	38.798776005569074
10	15.397830002988378	27.390049088433194	32.30128533674353	24.91083557183489
11	35.20562717568398	22.659414757703367	23.25792260574603	18.877035460866622
12	24.07152104361249	24.62887412840545	29.462643403927125	21.836961424054937
13	28.963120433183263	21.001235440445647	25.260666803937827	24.774977322433262
14	23.063408347890828	20.680097118576306	26.40110127813811	29.85539325539476
15	24.2089267582644	27.688516502367854	23.46001691266271	24.642539826705033
16	24.32671338415386	27.09823860642177	24.467500221196712	24.10754778822766
17	23.813450698014364	26.176714195133528	25.731950353159625	24.277884753692476
18	24.62493090081962	25.3773921113844	26.706628040676918	23.291048947119062
19	25.551861777971048	25.387436278163435	26.184913863017144	22.87578808084838
20	24.778929960916287	25.353111117046375	26.126578038335868	23.741380883701467
21	25.06828689342348	25.274524718213975	26.033679112529125	23.623509275833424
22	24.265720919699607	25.200766286509474	26.3932064683662	24.14030632542472
23	23.695842024010332	24.82636034308108	26.497780277984816	24.98001735492377
24	23.818622816764886	25.73419966269021	26.429129054102553	24.018048466442348
25	24.057166624239617	25.254972658896257	26.438144838992823	24.249715877871303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	828.0
1	828.0
2	1353.0
3	1878.0
4	1878.0
5	1878.0
6	4524.0
7	7170.0
8	7170.0
9	7170.0
10	9362.5
11	11555.0
12	11555.0
13	11555.0
14	15248.5
15	18942.0
16	18942.0
17	18942.0
18	31951.0
19	44960.0
20	44960.0
21	44960.0
22	65427.5
23	85895.0
24	85895.0
25	85895.0
26	116450.5
27	147006.0
28	147006.0
29	147006.0
30	174990.0
31	202974.0
32	202974.0
33	202974.0
34	235262.5
35	267551.0
36	267551.0
37	267551.0
38	295970.0
39	324389.0
40	324389.0
41	324389.0
42	353939.5
43	383490.0
44	383490.0
45	383490.0
46	406456.5
47	429423.0
48	429423.0
49	429423.0
50	432395.5
51	435368.0
52	435368.0
53	435368.0
54	407259.5
55	379151.0
56	379151.0
57	379151.0
58	342033.5
59	304916.0
60	304916.0
61	304916.0
62	263965.0
63	223014.0
64	223014.0
65	223014.0
66	183249.0
67	143484.0
68	143484.0
69	143484.0
70	111379.5
71	79275.0
72	79275.0
73	79275.0
74	62825.5
75	46376.0
76	46376.0
77	46376.0
78	38414.5
79	30453.0
80	30453.0
81	30453.0
82	22953.0
83	15453.0
84	15453.0
85	15453.0
86	11460.5
87	7468.0
88	7468.0
89	7468.0
90	5295.5
91	3123.0
92	3123.0
93	3123.0
94	1714.5
95	306.0
96	306.0
97	306.0
98	168.0
99	30.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32817004304936626
2	0.009959721550667442
3	0.0
4	0.0
5	0.04682181946864051
6	0.003338454151061712
7	0.0033106336998028644
8	3.060249638473236E-4
9	0.009570235233043574
10	0.015468170899919266
11	0.0057310129593226055
12	0.009653696586820117
13	0.017193038877967817
14	0.003032429187214388
15	0.020058545357629118
16	0.01090561689346826
17	0.005870115215616843
18	1.947431588119332E-4
19	0.004033965432532902
20	0.003950504078756359
21	0.0023369179057431983
22	0.0
23	0.003032429187214388
24	0.004339990396380225
25	0.0020308929418958745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3594478.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.5717752539799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.16256242858847	52.79591141985453
2	7.764403429785455	9.406073990603906
3	1.9133153620606111	3.476787243021675
4	0.8550641460893855	2.0717101313865
5	0.4791689437161224	1.4512056783729954
6	0.31857617564476615	1.157803470745632
7	0.22682236411569134	0.9617323283254436
8	0.16654576919850828	0.80703783211146
9	0.13790739691606677	0.7517966266675257
>10	0.8338754595002877	9.53115245916276
>50	0.07408491281481797	3.1195645233585387
>100	0.05607343539117559	6.95406423485479
>500	0.00796361301979543	3.2541026778954056
>1k	0.0034984658238000135	3.40266153089153
>5k	4.603244505000017E-5	0.15463728315912995
>10k+	9.206489010000034E-5	0.7037585695882509
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	15218	0.4233716272571427	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10022	0.27881656251617065	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5546	0.15429222268156878	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4591	0.12772369172936932	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4523	0.12583190104376768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.56409025176952E-5	0.0
6	2.78204512588476E-5	0.0	0.0	5.56409025176952E-5	0.0
7	2.78204512588476E-5	0.0	0.0	5.56409025176952E-5	0.0
8	2.78204512588476E-5	0.0	0.0	5.56409025176952E-5	0.0
9	2.78204512588476E-5	0.0	0.0	5.56409025176952E-5	0.0
10	2.78204512588476E-5	0.0	0.0	8.34613537765428E-5	0.0
11	2.78204512588476E-5	0.0	0.0	1.112818050353904E-4	0.0
12	2.78204512588476E-5	0.0	0.0	1.112818050353904E-4	2.78204512588476E-5
13	2.78204512588476E-5	0.0	0.0	1.112818050353904E-4	8.34613537765428E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1830	0.0	15.884157	1
GTATCAA	6155	0.0	13.3783045	1
GTCGTAT	50	0.0014644174	13.342691	1
GTATACG	60	3.9851357E-4	12.707325	1
AACCGCG	320	0.0	12.168639	7
ACGCTTA	110	3.84116E-8	12.086014	17
CGTCGTA	410	0.0	11.8150835	10
CGACCAT	375	0.0	11.39808	10
CGGTATA	60	0.0058721798	11.08486	2
ATACCGT	465	0.0	11.028865	6
TCGGCGT	250	0.0	11.018144	13
GCGTTAT	175	2.0008883E-11	10.891993	1
AGAACCG	430	0.0	10.8273735	5
CGGTTCT	465	0.0	10.826134	12
CCGACCA	400	0.0	10.6857	9
CACGCAA	80	3.7791487E-4	10.685699	11
CGAGCCG	540	0.0	10.553336	15
GTCGTAG	455	0.0	10.437802	11
GAACCGC	385	0.0	10.360449	6
TACCGTC	450	0.0	10.341695	7
>>END_MODULE