FastQCFastQC Report
Thu 2 Feb 2017
SRR4050264_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050264_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3553092
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN119120.3352572913957758No Hit
GTATCAACGCAGAGTACTTTTTTTT86950.2447164328984445No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN52760.1484903852756979No Hit
TATCAACGCAGAGTACTTTTTTTTT52350.14733646075024232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.015.3087531
GTATCAA53500.013.6135681
CGAGCCG5200.012.05693715
AACCGCG2450.011.6317597
GTCTTAA4400.011.4530231
CGGTTCT5400.011.43430612
CGTTATT2000.011.4012262
CGTCGTA3400.011.17545310
CGACCAT3250.011.10668110
GCGTGAA600.005881529811.08248111
AGGCCCG6200.011.03125310
CGAACGT2250.010.9777134
CGTTCAA1303.273817E-810.96085214
ATACCGT3950.010.8217996
GTATTAG4500.010.7759261
CCGTCGT3700.010.5259199
CAACGAC1002.4024042E-510.44934614
AGAACCG3200.010.3905635
CCGAGCC6250.010.33535214
CGGTCCA4600.010.32514610
Produced by FastQC (version 0.11.5)