##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050264_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3553092
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.028021509153156	31.0	31.0	34.0	26.0	34.0
2	30.007634758683423	31.0	31.0	34.0	26.0	34.0
3	30.181170653616626	31.0	31.0	34.0	26.0	34.0
4	33.648411299228954	37.0	35.0	37.0	28.0	37.0
5	33.716250522080486	37.0	35.0	37.0	28.0	37.0
6	33.77326452565821	37.0	35.0	37.0	30.0	37.0
7	33.71787755566138	37.0	35.0	37.0	29.0	37.0
8	33.675325603727686	37.0	35.0	37.0	29.0	37.0
9	35.180870633240005	39.0	35.0	39.0	29.0	39.0
10	35.05788000986183	39.0	35.0	39.0	28.0	39.0
11	35.0882003618257	39.0	35.0	39.0	28.0	39.0
12	35.01478346184113	39.0	35.0	39.0	28.0	39.0
13	35.007466173124705	39.0	35.0	39.0	28.0	39.0
14	36.08708837260617	40.0	36.0	41.0	27.0	41.0
15	36.07662706172539	40.0	36.0	41.0	27.0	41.0
16	36.076581467634384	40.0	36.0	41.0	27.0	41.0
17	36.04565769757721	40.0	36.0	41.0	27.0	41.0
18	35.979338277759204	40.0	36.0	41.0	27.0	41.0
19	36.02063892519529	40.0	37.0	41.0	27.0	41.0
20	35.995041501880614	40.0	37.0	41.0	27.0	41.0
21	35.915634044938884	40.0	37.0	41.0	27.0	41.0
22	35.82672500458755	40.0	36.0	41.0	27.0	41.0
23	35.755065165776735	40.0	36.0	41.0	27.0	41.0
24	35.70200996765634	40.0	36.0	41.0	27.0	41.0
25	35.363017338138164	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1784205650592767
1101	2	1.1568032074206194
1101	3	1.1754128663072478
1101	4	1.3031144829525445
1101	5	1.272050333913171
1101	6	1.2816439968374311
1101	7	1.2478538305601532
1101	8	1.2650362122016858
1101	9	1.4022360871887543
1101	10	1.3751593199173229
1101	11	1.4174123671416794
1101	12	1.4488279928912888
1101	13	1.4867466079910727
1101	14	1.646195306193718
1101	15	1.723073591656295
1101	16	1.7180153714084199
1101	17	1.7090945298827336
1101	18	1.6940947204988746
1101	19	1.6496839478339638
1101	20	1.6916502885523172
1101	21	1.6501916611746168
1101	22	1.691706950765898
1101	23	1.6725507481366932
1101	24	1.727970748088886
1101	25	1.6992023803268026
1102	1	1.0534857008842344
1102	2	1.1180929381036009
1102	3	1.0770069526848118
1102	4	1.1643427021152135
1102	5	1.1444910299861846
1102	6	1.1165980290494062
1102	7	1.0864212476752897
1102	8	1.0575842580538293
1102	9	1.211183489059195
1102	10	1.3439256952521887
1102	11	1.2333615189584393
1102	12	1.26862194597485
1102	13	1.2296901793610004
1102	14	1.4346329588102975
1102	15	1.435636786256623
1102	16	1.3860769023009993
1102	17	1.4355679268012693
1102	18	1.4730658675614876
1102	19	1.4446707063860984
1102	20	1.4381623779213442
1102	21	1.4402301302043696
1102	22	1.5084290286682744
1102	23	1.5046549095329098
1102	24	1.5113973340688318
1102	25	1.4412259974061428
1103	1	0.9209051232009529
1103	2	0.9943147677530284
1103	3	1.0372661139605484
1103	4	1.0228961261698828
1103	5	1.0320326486327147
1103	6	1.0359994362067297
1103	7	1.0743429805630385
1103	8	1.074906297868047
1103	9	1.2418873453683545
1103	10	1.2788949810488788
1103	11	1.2359746103605502
1103	12	1.1412315952754
1103	13	1.1830327169244867
1103	14	1.3506812054855075
1103	15	1.337518698375078
1103	16	1.39030796250794
1103	17	1.3954998642440088
1103	18	1.3883357181491007
1103	19	1.3528596315450514
1103	20	1.3625586262375862
1103	21	1.478112001279733
1103	22	1.498500566092595
1103	23	1.5484550482861579
1103	24	1.4982099549239365
1103	25	1.5569721807001216
1104	1	0.8131285850549297
1104	2	0.9556445462600003
1104	3	0.8959453592435693
1104	4	0.9913519095989258
1104	5	1.0307913032676623
1104	6	0.9954153508909016
1104	7	0.9373487229748463
1104	8	0.9691327129623843
1104	9	1.1199616685841036
1104	10	1.2069596241990013
1104	11	1.1454105414516107
1104	12	1.1177551391638332
1104	13	1.168132306752213
1104	14	1.3644434696364485
1104	15	1.2552166475137128
1104	16	1.3375378230493666
1104	17	1.3155942038666524
1104	18	1.3026187370170206
1104	19	1.2849917070167507
1104	20	1.277719577837253
1104	21	1.3446083097292814
1104	22	1.4067821083403373
1104	23	1.3340757209686913
1104	24	1.3591211936441994
1104	25	1.2998225498551648
1105	1	0.6401828052027341
1105	2	0.7755982607679748
1105	3	0.8167660515811797
1105	4	0.8942813533951153
1105	5	0.8278931313501232
1105	6	0.8205020875692384
1105	7	0.7771441333210163
1105	8	0.7372742921144706
1105	9	0.9908316024619737
1105	10	0.9954010088742251
1105	11	0.8639902380677142
1105	12	0.9063510940452062
1105	13	0.9442942670789307
1105	14	1.0833599223785768
1105	15	1.034335978513326
1105	16	1.0845083403578073
1105	17	1.02490770056054
1105	18	1.0557307556868025
1105	19	1.1628520726332425
1105	20	1.0197807213611725
1105	21	1.043487734077786
1105	22	1.1440295606935038
1105	23	1.0858126326761948
1105	24	1.0242603612832468
1105	25	0.9677527715587786
1106	1	0.5424939974456571
1106	2	0.6397802361117684
1106	3	0.6636116458266166
1106	4	0.7562730802564701
1106	5	0.7276810030015994
1106	6	0.6128908354975167
1106	7	0.5979925846244143
1106	8	0.6622197521109996
1106	9	0.7505517864100284
1106	10	0.8295330943624393
1106	11	0.8650579758871828
1106	12	0.9234382254428155
1106	13	0.8597995055359462
1106	14	0.979436324064423
1106	15	1.0195282400640764
1106	16	1.0127157781994995
1106	17	1.0454858800354074
1106	18	1.0153177107912512
1106	19	1.0371464010494407
1106	20	1.1123266473925568
1106	21	1.0261586708087194
1106	22	1.1192926366565672
1106	23	1.0391428437356964
1106	24	1.032013934047086
1106	25	0.9820515882436069
1107	1	0.43406141807556864
1107	2	0.4461192179297946
1107	3	0.480729599393662
1107	4	0.4963552867096155
1107	5	0.437249839853024
1107	6	0.38913767734493376
1107	7	0.3497727379842317
1107	8	0.2822959399792708
1107	9	0.3178824941000542
1107	10	0.42276525271682175
1107	11	0.3223959073052711
1107	12	0.28853300032706386
1107	13	0.3213127945570875
1107	14	0.3810787416814776
1107	15	0.36869394582515724
1107	16	0.3937997367454287
1107	17	0.3856411081818436
1107	18	0.39224913101327274
1107	19	0.4656990240899219
1107	20	0.4868783020398837
1107	21	0.46250136789063134
1107	22	0.5246687875148197
1107	23	0.43501155586550055
1107	24	0.37055533998565693
1107	25	0.29505294385140957
1108	1	0.5345446337544324
1108	2	0.6274672726942399
1108	3	0.5866721624164128
1108	4	0.6678491610285491
1108	5	0.7224217773499362
1108	6	0.564516623718788
1108	7	0.5367794553120575
1108	8	0.5952334323868484
1108	9	0.653464085112418
1108	10	0.7729922381078751
1108	11	0.7775094899850927
1108	12	0.7315072513202665
1108	13	0.7352882558390945
1108	14	0.7804007099980055
1108	15	0.7232199310407381
1108	16	0.6348042314610964
1108	17	0.7349624016161442
1108	18	0.7653748705717192
1108	19	0.7827096557346991
1108	20	0.7335689045297471
1108	21	0.8279082543889373
1108	22	0.7716009978441178
1108	23	0.638904276585734
1108	24	0.7012080410893162
1108	25	0.7256772999012782
1109	1	0.2747403561327424
1109	2	0.382762979190133
1109	3	0.2780305731811019
1109	4	0.37064860575346614
1109	5	0.3762253527350765
1109	6	0.23596093673671703
1109	7	0.24320284826822558
1109	8	0.28666800814202276
1109	9	0.2413011522454127
1109	10	0.37035828821777983
1109	11	0.3772530400975782
1109	12	0.3687393962499499
1109	13	0.24498220745631016
1109	14	0.3267990933952518
1109	15	0.27391356031385783
1109	16	0.36848759737686265
1109	17	0.36430425983343184
1109	18	0.4398563250293037
1109	19	0.4407799359389415
1109	20	0.38695099144982237
1109	21	0.4540993730981171
1109	22	0.5065742729909957
1109	23	0.38364791811618915
1109	24	0.34295856501086774
1109	25	0.3567962635218933
1110	1	0.14138058987337843
1110	2	0.34835509521887076
1110	3	0.2902200107665429
1110	4	0.30206187346056623
1110	5	0.3472464400771642
1110	6	0.2819267947043542
1110	7	0.18236231788105783
1110	8	0.1906732395498949
1110	9	0.2376884970129467
1110	10	0.24311773812087267
1110	11	0.30448202656604906
1110	12	0.23153386915867458
1110	13	0.259729002673744
1110	14	0.2867945623915702
1110	15	0.1847757251251423
1110	16	0.17246971084836815
1110	17	0.2423615416742564
1110	18	0.22538435395042455
1110	19	0.2678240938692724
1110	20	0.19898738028089724
1110	21	0.23493338286643706
1110	22	0.2498678422931846
1110	23	0.2115975932798193
1110	24	0.2116775178568986
1110	25	0.2812943447269589
1111	1	-0.14547577589777205
1111	2	-0.016072662730270082
1111	3	-0.18599694199762595
1111	4	-0.08366734568453893
1111	5	-0.03395314490409618
1111	6	-0.1461888087870591
1111	7	-0.2362326255659113
1111	8	-0.18359724342808903
1111	9	-0.1934591298056958
1111	10	-0.19060132850143674
1111	11	-0.1278090492897519
1111	12	-0.11390463943395446
1111	13	-0.16185212800122883
1111	14	-0.20512506441252754
1111	15	-0.14789177247957497
1111	16	-0.1485697918449631
1111	17	-0.1558600061299984
1111	18	-0.13768283963510441
1111	19	-0.20525686728717574
1111	20	-0.17643298841567656
1111	21	-0.11972949637605268
1111	22	-0.05167639551743264
1111	23	-0.16230415155730782
1111	24	-0.14246860843564235
1111	25	-0.18382233441186457
1112	1	-0.30889236290662225
1112	2	-0.17914928867536517
1112	3	-0.36139305640111274
1112	4	-0.2931316170832119
1112	5	-0.25591814320234363
1112	6	-0.3118945596162277
1112	7	-0.42626480039273673
1112	8	-0.43597699867168416
1112	9	-0.4402842048000508
1112	10	-0.5168668259170062
1112	11	-0.4346070919590659
1112	12	-0.4791008767460099
1112	13	-0.4029068712391677
1112	14	-0.602993255587279
1112	15	-0.5810754970007039
1112	16	-0.5009201152999836
1112	17	-0.5085970457190285
1112	18	-0.49042832960545013
1112	19	-0.4760845276534553
1112	20	-0.3910313668097416
1112	21	-0.39420077214012395
1112	22	-0.37855882636301175
1112	23	-0.5302659429358556
1112	24	-0.5613476164320375
1112	25	-0.6398618386102015
1113	1	-0.45515884506805193
1113	2	-0.3843984181226858
1113	3	-0.4943069966101312
1113	4	-0.419867448331118
1113	5	-0.4159934276060042
1113	6	-0.45482933845585194
1113	7	-0.5663928518534576
1113	8	-0.5231573365504687
1113	9	-0.5445998072184821
1113	10	-0.5853778937693761
1113	11	-0.5146206995642046
1113	12	-0.4101224842190092
1113	13	-0.5290009946373431
1113	14	-0.5613076563817572
1113	15	-0.6288150087712268
1113	16	-0.6751551652911516
1113	17	-0.6163344017873982
1113	18	-0.631474698536401
1113	19	-0.7239063923284021
1113	20	-0.7386792082991462
1113	21	-0.73034313572672
1113	22	-0.6977042037503836
1113	23	-0.7515496423918009
1113	24	-0.9009387328851091
1113	25	-0.9111177791657568
1114	1	-0.6314140860582995
1114	2	-0.7293630764174637
1114	3	-0.8019250552395185
1114	4	-0.769534036089496
1114	5	-0.7706726319395827
1114	6	-0.8732544918913803
1114	7	-0.7541713659563882
1114	8	-0.9049590767787592
1114	9	-1.0348380455654862
1114	10	-0.9561307998124349
1114	11	-0.8894316373636997
1114	12	-1.2616602332357232
1114	13	-1.0544170342038015
1114	14	-1.228537410147574
1114	15	-1.2175242449747756
1114	16	-1.0915572380908571
1114	17	-1.4314258056616964
1114	18	-1.2007071324446272
1114	19	-1.156321140370892
1114	20	-1.2133024326439283
1114	21	-1.1432536880474373
1114	22	-1.2106385046499781
1114	23	-1.3252240725838007
1114	24	-1.1812234784218631
1114	25	-1.3522307771259392
1115	1	-1.0244638055601634
1115	2	-0.9695697847482414
1115	3	-1.1626088576239013
1115	4	-1.1463213941423547
1115	5	-1.0756033701629022
1115	6	-1.1604971411826597
1115	7	-1.0933944602845074
1115	8	-1.1631412248943036
1115	9	-1.837289948410188
1115	10	-1.49505457998616
1115	11	-1.5185648548705188
1115	12	-1.502722096092981
1115	13	-1.4643415042813928
1115	14	-1.6593958455189082
1115	15	-1.6485597633930738
1115	16	-1.7785025066906428
1115	17	-1.6023734562474914
1115	18	-1.5199957208143005
1115	19	-1.5664521737695551
1115	20	-1.5882662179775835
1115	21	-1.57733018107605
1115	22	-1.7463704598270695
1115	23	-1.580669237813808
1115	24	-1.7971542660894713
1115	25	-1.6773377545202308
1116	1	-1.561423422189261
1116	2	-1.6509346870632697
1116	3	-1.451537284885621
1116	4	-1.782552467098931
1116	5	-1.405753701259954
1116	6	-1.552367927104875
1116	7	-1.5684641354616176
1116	8	-1.5628998858906158
1116	9	-2.087771564135487
1116	10	-2.846668990349748
1116	11	-2.1870237477122885
1116	12	-1.9238504750830714
1116	13	-1.9453065100301572
1116	14	-2.1100139405657856
1116	15	-2.9161605332891867
1116	16	-2.39723320019241
1116	17	-2.239360519948626
1116	18	-2.2953731132697257
1116	19	-2.1243542030345637
1116	20	-2.2321996494491927
1116	21	-2.1721870419157554
1116	22	-2.2741989963985674
1116	23	-2.286115404899107
1116	24	-2.1042659479193375
1116	25	-2.3202481413612546
1201	1	1.0206070833484446
1201	2	1.0310558090586355
1201	3	0.9923702739193345
1201	4	1.0589723874146273
1201	5	1.0180622930287555
1201	6	1.0502242246110356
1201	7	1.0053930915755274
1201	8	0.981584931392419
1201	9	1.1446213955465652
1201	10	1.2570190098645284
1201	11	1.2056057632946136
1201	12	1.1832810777303848
1201	13	1.24369271630853
1201	14	1.4484659952337182
1201	15	1.45993132328163
1201	16	1.4547207582029529
1201	17	1.3248860127403717
1201	18	1.436093139747399
1201	19	1.3734541643546336
1201	20	1.443363263844084
1201	21	1.3900929513675067
1201	22	1.44044456567822
1201	23	1.4504914206273938
1201	24	1.5441464496168962
1201	25	1.5427365430292213
1202	1	0.8997446654569394
1202	2	0.9603463888060659
1202	3	0.8413138684562966
1202	4	0.8160691625302476
1202	5	0.8760117387785513
1202	6	0.8769245636212162
1202	7	0.8409467129245982
1202	8	0.926325342778135
1202	9	0.8840005295556281
1202	10	0.9651415337467384
1202	11	0.8957351645671849
1202	12	1.0410908175557978
1202	13	0.957121921492579
1202	14	1.186025379184187
1202	15	1.149547635788423
1202	16	1.087660771123069
1202	17	1.0258370849386793
1202	18	1.15036915577749
1202	19	1.1089079549229481
1202	20	1.1182043349561823
1202	21	1.259403954620403
1202	22	1.255462845358764
1202	23	1.2247695233137463
1202	24	1.2532830026894217
1202	25	1.31912774248498
1203	1	0.8260966611220972
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2211	7	-0.48873233072322364
2211	8	-0.4647842069970878
2211	9	-0.3986129345904956
2211	10	-0.4918803622920507
2211	11	-0.5007480536176203
2211	12	-0.4778212877189745
2211	13	-0.4673866395497157
2211	14	-0.5931005357671282
2211	15	-0.4966449532968227
2211	16	-0.41718382504249263
2211	17	-0.616457467865871
2211	18	-0.6825751164108738
2211	19	-0.6916752973736564
2211	20	-0.7546454339830788
2211	21	-0.6263376544720032
2211	22	-0.5623326501871873
2211	23	-0.612895032155258
2211	24	-0.47106293333992966
2211	25	-0.5470121006007602
2212	1	-0.605185394149558
2212	2	-0.49229587295108956
2212	3	-0.4235842661071487
2212	4	-0.48019184349564625
2212	5	-0.5704935292129463
2212	6	-0.5059640846595244
2212	7	-0.5137478276110556
2212	8	-0.48178856117525726
2212	9	-0.5740321587801489
2212	10	-0.5663361011682184
2212	11	-0.6457788297317535
2212	12	-0.584798780624908
2212	13	-0.5016218440977624
2212	14	-0.5967726056234923
2212	15	-0.6749602463631632
2212	16	-0.8005749348314737
2212	17	-0.7375160453493947
2212	18	-0.7291886569423767
2212	19	-0.7177495044042743
2212	20	-0.6937052777494728
2212	21	-0.743071010290528
2212	22	-0.6371636252790722
2212	23	-0.6631759047121193
2212	24	-0.7102283359811565
2212	25	-0.8091707823778762
2213	1	-0.972403999214734
2213	2	-0.7546190915839972
2213	3	-0.8438315500545919
2213	4	-0.9206813748824274
2213	5	-0.9147640134560007
2213	6	-0.9208103452497909
2213	7	-0.9416621873003521
2213	8	-1.036317213759645
2213	9	-0.9430527264655595
2213	10	-1.020469328740262
2213	11	-0.991098756708297
2213	12	-1.0341070656887936
2213	13	-1.0909915492972928
2213	14	-1.18441694397697
2213	15	-1.2017933182899014
2213	16	-1.2834253425588429
2213	17	-1.2498194095513568
2213	18	-1.1942187487344853
2213	19	-1.217791390198684
2213	20	-1.2405505410358941
2213	21	-1.2381878146804226
2213	22	-1.2510086097837458
2213	23	-1.1964131896321888
2213	24	-1.23570883403859
2213	25	-1.254987643924352
2214	1	-1.0118133671105518
2214	2	-1.0378868047419374
2214	3	-0.9843002774726557
2214	4	-0.9185697452168498
2214	5	-1.0256847148318826
2214	6	-0.9908574351465944
2214	7	-1.1861173403735705
2214	8	-1.1122544880718763
2214	9	-1.102969036005426
2214	10	-1.6207911191752729
2214	11	-1.46031098408168
2214	12	-1.3739246281276962
2214	13	-1.3706803629310471
2214	14	-1.584458017112098
2214	15	-1.5705101786013458
2214	16	-1.650478725108357
2214	17	-1.619754858835357
2214	18	-1.5287923812764461
2214	19	-1.5536941365514494
2214	20	-1.6467572159571873
2214	21	-1.5799663509042077
2214	22	-1.4379803997398213
2214	23	-1.5422725660384202
2214	24	-1.5475371127552862
2214	25	-1.4416640694079277
2215	1	-1.7645912189596125
2215	2	-1.4564880600304981
2215	3	-1.5496987058741816
2215	4	-2.179583889357925
2215	5	-1.7778438160621945
2215	6	-1.5594880788546348
2215	7	-1.5414693582642869
2215	8	-1.7052146870692226
2215	9	-1.9069445745010825
2215	10	-1.8137099506389944
2215	11	-1.8517744119228183
2215	12	-1.9440107214336066
2215	13	-1.9585092611314607
2215	14	-2.048136678933176
2215	15	-2.1155681478079202
2215	16	-2.1782351499236086
2215	17	-2.1671203527952727
2215	18	-2.4226875692892804
2215	19	-2.8156052583696862
2215	20	-2.455757520313526
2215	21	-2.2843464871728827
2215	22	-3.099985964996158
2215	23	-2.4123606311729304
2215	24	-2.4761319120931162
2215	25	-2.333239217175027
2216	1	-2.9061120375371488
2216	2	-5.666445298091979
2216	3	-3.305492458602018
2216	4	-5.7823351916493415
2216	5	-3.3992411824774464
2216	6	-2.309642131001535
2216	7	-2.125153049255079
2216	8	-1.883351799289116
2216	9	-2.167683669680649
2216	10	-2.1108820771417314
2216	11	-2.051606626062643
2216	12	-2.190444614265047
2216	13	-2.1066632549676214
2216	14	-2.8963551434009958
2216	15	-2.68362258811581
2216	16	-2.683301936543792
2216	17	-2.501164156435401
2216	18	-2.4406222862531237
2216	19	-2.529854802460825
2216	20	-2.816521013933432
2216	21	-2.9902628568603333
2216	22	-2.929326876094862
2216	23	-2.723868430672553
2216	24	-2.8446952341566956
2216	25	-3.1289265023870954
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	129267.0
3	9.0
4	876.0
5	5277.0
6	7123.0
7	6603.0
8	5965.0
9	5492.0
10	5058.0
11	4972.0
12	4721.0
13	4559.0
14	4494.0
15	4605.0
16	4716.0
17	5147.0
18	5738.0
19	6936.0
20	9221.0
21	11796.0
22	14806.0
23	17438.0
24	20244.0
25	23429.0
26	26004.0
27	29244.0
28	33562.0
29	39805.0
30	50474.0
31	64040.0
32	82996.0
33	109174.0
34	149617.0
35	210521.0
36	312967.0
37	561307.0
38	1574889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.958589071475565	17.953552301699798	12.4927376019279	25.595121024896745
2	16.024015390527843	20.714379645868217	38.45418495160985	24.80742001199409
3	18.367521641288224	24.91000926056369	29.16618360747002	27.556285490678068
4	12.64265696163705	16.756682744071043	38.090237952010455	32.51042234228146
5	14.973512947256957	35.89917767517815	33.996731113533556	15.130578264031334
6	31.79833315566362	37.31157559770738	17.079427434774423	13.810663811854576
7	28.229612565587548	31.22219689081559	22.18645578837765	18.361734755219214
8	26.080284936360265	33.49363177328483	20.56080542948707	19.865277860867835
9	26.859485857072148	15.223542005802662	19.37357435306885	38.543397784056346
10	15.4940565130882	27.045905722319407	32.411754817593504	25.04828294699889
11	35.15598642082624	22.647162055882823	23.34715746296035	18.849694060330588
12	24.12631041741978	24.42868990154592	29.510252975241176	21.934746705793128
13	28.851936593262963	21.00454484884054	25.232133187036265	24.91138537086023
14	23.147330580635504	20.711811137934887	26.19696137092745	29.94389691050216
15	24.278042570324395	27.633198842032737	23.326036967622848	24.762721620020017
16	24.418474428695408	27.09659804386601	24.376938406757585	24.107989120680998
17	23.918312954102426	26.15658337763601	25.606634768740033	24.31846889952153
18	24.790464122075935	25.45062370931882	26.393793898755302	23.365118269849944
19	25.73831813861485	25.586191369520883	25.705652707487868	22.969837784376395
20	24.930704803597454	25.543530652633727	25.706788320753972	23.81897622301485
21	25.22806306236063	25.456161565756585	25.544537331460155	23.771238040422627
22	24.371868843310878	25.409226654628807	25.966412336686467	24.252492165373845
23	23.794124426608917	25.02628972768407	26.09021211252599	25.089373733181024
24	23.87774938004801	25.91504233479619	26.015272396102397	24.191935889053404
25	24.135193376431893	25.43226154020642	25.975275036860612	24.45727004650108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16660.0
1	16660.0
2	13516.0
3	10372.0
4	10372.0
5	10372.0
6	8030.5
7	5689.0
8	5689.0
9	5689.0
10	8000.0
11	10311.0
12	10311.0
13	10311.0
14	14762.5
15	19214.0
16	19214.0
17	19214.0
18	31524.5
19	43835.0
20	43835.0
21	43835.0
22	62495.0
23	81155.0
24	81155.0
25	81155.0
26	110161.5
27	139168.0
28	139168.0
29	139168.0
30	166319.0
31	193470.0
32	193470.0
33	193470.0
34	227723.0
35	261976.0
36	261976.0
37	261976.0
38	289976.5
39	317977.0
40	317977.0
41	317977.0
42	347684.0
43	377391.0
44	377391.0
45	377391.0
46	400101.0
47	422811.0
48	422811.0
49	422811.0
50	425942.5
51	429074.0
52	429074.0
53	429074.0
54	401618.0
55	374162.0
56	374162.0
57	374162.0
58	338670.5
59	303179.0
60	303179.0
61	303179.0
62	262195.5
63	221212.0
64	221212.0
65	221212.0
66	182061.0
67	142910.0
68	142910.0
69	142910.0
70	111457.0
71	80004.0
72	80004.0
73	80004.0
74	63091.0
75	46178.0
76	46178.0
77	46178.0
78	38158.0
79	30138.0
80	30138.0
81	30138.0
82	22617.5
83	15097.0
84	15097.0
85	15097.0
86	11366.0
87	7635.0
88	7635.0
89	7635.0
90	5386.0
91	3137.0
92	3137.0
93	3137.0
94	1701.0
95	265.0
96	265.0
97	265.0
98	168.5
99	72.0
100	72.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4029166708883418
2	0.6948314313279814
3	0.7100576061638708
4	0.7197674588780701
5	0.7288581325786104
6	0.7302935021102747
7	0.7460544224579605
8	0.7353876567226517
9	0.7246083129848594
10	0.7162493963004617
11	0.7296743230966155
12	0.7336426976841579
13	0.7323761951562189
14	0.7421704813722808
15	0.7380050952803924
16	0.7326857846630485
17	0.7378643727772881
18	0.74214233687166
19	0.7438028624082912
20	0.7360349802369316
21	0.7347684777089927
22	0.7441405964157415
23	0.7331360966729823
24	0.7375547832704585
25	0.7399752103238532
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3553092.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.707443516763504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.25100790251993	52.96785634022915
2	7.633852064307396	9.268632860185395
3	1.938156116015134	3.5298150881897543
4	0.8491572949606679	2.0620067408268974
5	0.49243528249626217	1.4947243548901656
6	0.33140994821514974	1.2071430427298833
7	0.22303089002824206	0.9477744611218096
8	0.17755286807375179	0.8623024567861318
9	0.1400437849601286	0.7651530148806703
>10	0.8217038618557474	9.425574941339475
>50	0.07548366976455137	3.1798863601011487
>100	0.05504501933771987	6.923576201517041
>500	0.0072428617996329134	2.988426763844839
>1k	0.0036915231036961527	3.494388767618638
>5k	1.4018442165934756E-4	0.5448254277853564
>10k+	4.6728140553115855E-5	0.337913177953721
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	11912	0.3352572913957758	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8695	0.2447164328984445	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5276	0.1484903852756979	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5235	0.14733646075024232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	0.0
3	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	0.0
4	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	0.0
5	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	0.0
6	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	0.0
7	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	0.0
8	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	0.0
9	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	2.8144500620867684E-5
10	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	2.8144500620867684E-5
11	2.8144500620867684E-5	2.8144500620867684E-5	0.0	0.0	2.8144500620867684E-5
12	5.628900124173537E-5	2.8144500620867684E-5	0.0	0.0	2.8144500620867684E-5
13	5.628900124173537E-5	2.8144500620867684E-5	0.0	0.0	2.8144500620867684E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1590	0.0	15.308753	1
GTATCAA	5350	0.0	13.613568	1
CGAGCCG	520	0.0	12.056937	15
AACCGCG	245	0.0	11.631759	7
GTCTTAA	440	0.0	11.453023	1
CGGTTCT	540	0.0	11.434306	12
CGTTATT	200	0.0	11.401226	2
CGTCGTA	340	0.0	11.175453	10
CGACCAT	325	0.0	11.106681	10
GCGTGAA	60	0.0058815298	11.082481	11
AGGCCCG	620	0.0	11.031253	10
CGAACGT	225	0.0	10.977713	4
CGTTCAA	130	3.273817E-8	10.960852	14
ATACCGT	395	0.0	10.821799	6
GTATTAG	450	0.0	10.775926	1
CCGTCGT	370	0.0	10.525919	9
CAACGAC	100	2.4024042E-5	10.449346	14
AGAACCG	320	0.0	10.390563	5
CCGAGCC	625	0.0	10.335352	14
CGGTCCA	460	0.0	10.325146	10
>>END_MODULE