FastQCFastQC Report
Thu 2 Feb 2017
SRR4050264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3553092
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT150300.4230118443316413No Hit
TATCAACGCAGAGTACTTTTTTTTT99880.28110727220122644No Hit
GGTATCAACGCAGAGTACTTTTTTT56530.15910086200976503No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45120.126987986801355No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43930.12363879122747173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.016.1268621
CTCGACG953.092282E-1014.98897713
GTATCAA58250.013.1894651
CGCGAAC802.0180523E-613.052912
TCGACGC1102.7812348E-912.94502514
CGAACGA1900.012.48975916
ACGCGAA1052.7495662E-711.753262511
AACGAAC1950.011.68371614
ACGAACG2050.011.57603715
ACGCTTA1157.1648174E-811.55574317
CGTCGTA2800.011.52723810
CGACCAT3300.011.21902310
GACGCTT1451.2369128E-911.12880616
GCGTTAT2100.010.9073491
GATAACG2100.010.84916511
ACGAAAC700.001502456310.848248517
CCGATAA2400.010.6796469
CGACCGT803.8014352E-410.67889419
GCGAACC909.593285E-510.54779913
ACGGTAT4700.010.5029149
Produced by FastQC (version 0.11.5)