FastQCFastQC Report
Thu 2 Feb 2017
SRR4050263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175259
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT142680.6559218925194654No Hit
TATCAACGCAGAGTACTTTTTTTTT91500.42063956521958995No Hit
GTACTTTTTTTTTTTTTTTTTTTTT66730.30676806761861464No Hit
GGTATCAACGCAGAGTACTTTTTTT56100.2579003235936502No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN48290.22199655305414207No Hit
GAGTACTTTTTTTTTTTTTTTTTTT46820.21523873708831914No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46430.21344584713820286No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN37060.17037051679822954No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24200.11125111998157461No Hit
GTACAAAGGGCAGGGACTTAATCAA24150.11102126229566227No Hit
GAATAGGACCGCGGTTCTATTTTGT23900.10987197386610054No Hit
GCAGAGTACTTTTTTTTTTTTTTTT23830.10955017310582327No Hit
GTATCTGATCGTCTTCGAACCTCCG23340.10729756778388229No Hit
CTATTGGAGCTGGAATTACCGCGGC23220.10674590933769267No Hit
GTCCTATTCCATTATTCCTAGCTGC22690.10430941786702182No Hit
GATTAAGAGGGACGGCCGGGGGCAT22210.10210278408226332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG508.7126464E-515.2007781
CGCAGAT1550.012.8701368
AACGGAC604.093007E-412.66672815
ACTCTAA3600.011.87423310
GTATCAA78600.011.8091281
GTTCTAA1459.822543E-1111.7937081
TAAGGGT2500.011.780334
GCGTTAT1552.5465852E-1111.6457571
CTCGTAG2000.011.39926310
CGGTCCA4100.011.35292510
GTATAGA1354.7275535E-911.2598361
CGAGACT1354.7293724E-911.2595754
GCTCGTA1950.011.2044069
AGGCCCG5200.011.14351110
CGAGTGT600.005879741611.0826188
CGAACGA1900.011.00030816
CTTACTC951.3603423E-511.0003083
CACTCTA4350.010.9188369
ACCGGTC1053.4703335E-610.85644158
CTGTCGA1855.456968E-1210.7830889
Produced by FastQC (version 0.11.5)