##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050263_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175259 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.68451572893159 31.0 30.0 34.0 25.0 34.0 2 29.757280857130116 31.0 30.0 34.0 25.0 34.0 3 29.875108205505644 31.0 30.0 34.0 25.0 34.0 4 33.182321737319555 37.0 35.0 37.0 26.0 37.0 5 33.31194446270536 37.0 35.0 37.0 27.0 37.0 6 33.36495883938419 37.0 35.0 37.0 28.0 37.0 7 33.19243685464581 37.0 35.0 37.0 27.0 37.0 8 33.078566276475584 37.0 35.0 37.0 26.0 37.0 9 34.45892512110052 39.0 35.0 39.0 25.0 39.0 10 34.38566441973117 39.0 35.0 39.0 25.0 39.0 11 34.41302667866217 39.0 35.0 39.0 25.0 39.0 12 34.30345949608759 39.0 35.0 39.0 25.0 39.0 13 34.26578076449747 39.0 35.0 39.0 25.0 39.0 14 35.0893135024381 40.0 36.0 41.0 21.0 41.0 15 35.204316359569134 40.0 36.0 41.0 24.0 41.0 16 35.25715788326816 40.0 36.0 41.0 25.0 41.0 17 35.268303222742674 40.0 36.0 41.0 25.0 41.0 18 35.08766312425325 40.0 36.0 41.0 23.0 41.0 19 35.22121963407576 40.0 36.0 41.0 24.0 41.0 20 35.1998267792479 40.0 36.0 41.0 24.0 41.0 21 35.16809308684621 40.0 36.0 41.0 24.0 41.0 22 35.10123116373728 40.0 36.0 41.0 24.0 41.0 23 34.979860788991104 40.0 35.0 41.0 23.0 41.0 24 34.92358794975679 40.0 35.0 41.0 23.0 41.0 25 34.64608306413167 39.0 35.0 41.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0759806891365606 1101 2 0.9978797687340588 1101 3 1.015922045227061 1101 4 1.1004235641552285 1101 5 1.013943366302371 1101 6 0.9696505034557177 1101 7 1.0511074496375201 1101 8 1.0154690741683439 1101 9 1.0913778755778623 1101 10 1.122818523568398 1101 11 1.1282897831510184 1101 12 1.1618959799608177 1101 13 1.1546358091444162 1101 14 1.5703472671957996 1101 15 1.4613246649457423 1101 16 1.4296451881834216 1101 17 1.5199952041096907 1101 18 1.5774710757385861 1101 19 1.495488175608493 1101 20 1.5416045146864832 1101 21 1.4917496911266284 1101 22 1.5468281969767617 1101 23 1.516640358564807 1101 24 1.6350217085572893 1101 25 1.557953136375211 1102 1 1.2086977912581247 1102 2 1.183605099182767 1102 3 1.078439302624595 1102 4 1.238533095436189 1102 5 1.16210604224662 1102 6 1.2179215152508434 1102 7 1.1317396957142023 1102 8 1.1962851825886531 1102 9 1.3634982047109432 1102 10 1.505322040016928 1102 11 1.4097485976611992 1102 12 1.4567440986202485 1102 13 1.5091051908666842 1102 14 1.750383747303907 1102 15 1.6445170154926245 1102 16 1.6696675700128836 1102 17 1.654278059758262 1102 18 1.7121413092047817 1102 19 1.5953886105761228 1102 20 1.6917122008471068 1102 21 1.7009970574447308 1102 22 1.7171777953057585 1102 23 1.794489331566922 1102 24 1.8269710716984164 1102 25 1.7379197747803516 1103 1 0.8083988505242559 1103 2 0.8124670389652415 1103 3 0.7133664377415059 1103 4 0.8302594748815011 1103 5 0.7530767281419628 1103 6 0.8023445034376522 1103 7 0.7568106551739078 1103 8 0.8196243580496088 1103 9 0.9421696899621352 1103 10 0.8939873611795548 1103 11 0.9172941181820349 1103 12 1.0350189133573409 1103 13 0.9961087984390247 1103 14 1.1669827289999901 1103 15 1.0749371218363208 1103 16 1.043128029623837 1103 17 1.0253300685969648 1103 18 1.054775398985349 1103 19 0.904352683686426 1103 20 1.0153167526138205 1103 21 0.9591004850555436 1103 22 0.9481674651889591 1103 23 0.9335267371354163 1103 24 1.017210694055791 1103 25 0.9945314622259431 1104 1 0.8423431174493707 1104 2 0.8574238162951566 1104 3 0.7865897061900995 1104 4 1.0207800715037862 1104 5 0.9460000751924156 1104 6 0.8129621770668933 1104 7 0.8291860844844976 1104 8 0.8461908894038359 1104 9 0.9696322010927005 1104 10 0.9798111545386305 1104 11 1.013786754178554 1104 12 1.0436652226255418 1104 13 1.0220696558185765 1104 14 1.3868980411586094 1104 15 1.2429564081664992 1104 16 1.095058829492551 1104 17 1.037210781316503 1104 18 1.2991311179939729 1104 19 1.217523617726549 1104 20 1.2324162029229129 1104 21 1.1825577368474782 1104 22 1.3223523246135969 1104 23 1.2463036698123489 1104 24 1.181103669793643 1104 25 1.2664719758520917 1105 1 0.5435562402944036 1105 2 0.6014749524856242 1105 3 0.5464980558848751 1105 4 0.6173837404654776 1105 5 0.6134180082528928 1105 6 0.6266514213795489 1105 7 0.6000282703668844 1105 8 0.6674266839506373 1105 9 0.7887142822880691 1105 10 0.8483073207007692 1105 11 0.7079278004785792 1105 12 0.7443525507566378 1105 13 0.7258499113815446 1105 14 1.0911772511294373 1105 15 0.9835012958747242 1105 16 1.0492975823719846 1105 17 1.0003212876741188 1105 18 1.0345999434208863 1105 19 0.8933795334578178 1105 20 0.857849506728634 1105 21 0.8898023582172812 1105 22 0.9375758905989642 1105 23 0.9471909729711498 1105 24 0.9483014730178212 1105 25 0.9105055134535931 1106 1 0.5371428383211807 1106 2 0.5064799077465594 1106 3 0.5317778604331345 1106 4 0.717667923971689 1106 5 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2215 9 -2.4368809137557363 2215 10 -1.9244534996287754 2215 11 -2.2345402527971316 2215 12 -2.2555119743540075 2215 13 -2.2697288697600158 2215 14 -2.979437580461976 2215 15 -2.751213936738992 2215 16 -2.4506463160634055 2215 17 -2.3616198264614 2215 18 -2.270410549649533 2215 19 -2.462418667136447 2215 20 -2.7978128034091228 2215 21 -2.5662086127553536 2215 22 -2.815782930852613 2215 23 -2.614861928726441 2215 24 -2.617967772935593 2215 25 -2.577148817794985 2216 1 -1.4069750627722435 2216 2 -1.31680043301861 2216 3 -1.299318865384631 2216 4 -1.1031310027336474 2216 5 -1.727790071838566 2216 6 -1.4093107104355909 2216 7 -1.4272152580263509 2216 8 -1.829432033862549 2216 9 -1.8497608050331849 2216 10 -1.7303648694338989 2216 11 -1.855321410600908 2216 12 -1.9919363882001448 2216 13 -2.0168712102641138 2216 14 -1.8550862421772507 2216 15 -1.8851294331282276 2216 16 -1.9782026854307517 2216 17 -1.9893492534981405 2216 18 -2.3231606286408777 2216 19 -2.0263000164228586 2216 20 -2.091969880082125 2216 21 -2.1843433510560715 2216 22 -2.3419513365978517 2216 23 -2.061590849457197 2216 24 -2.2079762621261523 2216 25 -2.331824001568826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90172.0 3 8.0 4 593.0 5 3924.0 6 6826.0 7 6996.0 8 5871.0 9 4569.0 10 4600.0 11 5026.0 12 4965.0 13 4188.0 14 3720.0 15 3569.0 16 3445.0 17 3703.0 18 4613.0 19 6367.0 20 8521.0 21 10463.0 22 12236.0 23 13863.0 24 15386.0 25 16600.0 26 18530.0 27 20285.0 28 22528.0 29 25987.0 30 32230.0 31 41035.0 32 52525.0 33 69156.0 34 93131.0 35 130557.0 36 193041.0 37 336225.0 38 899805.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.92370654065053 18.275043496210667 12.668547816342778 24.132702146796024 2 16.554047176184262 21.65482172436449 38.135393242830574 23.655737856620675 3 18.35192285537279 26.426809627162374 29.3938999964374 25.827367521027433 4 12.083305763097096 16.91226927182649 38.66826273270629 32.33616223237013 5 14.894077846708488 36.342301524677374 32.96274270693289 15.800877921681241 6 30.61910931091637 38.49587157088591 16.26294198537658 14.622077132821138 7 27.567518031961374 32.362233223240864 21.80367748337505 18.26657126142271 8 26.308915486224148 32.758627871232534 20.08496316704625 20.84749347549707 9 27.41804412876737 15.097336335844371 19.594161208186748 37.89045832720151 10 16.325740098582624 26.66497595596501 30.644517122379533 26.364766823072834 11 34.69081168239135 23.871881713612396 22.865829107122526 18.57147749687373 12 24.556406768710424 25.573712678303906 28.534687951675075 21.33519260131059 13 28.779902656558697 21.90380150342257 25.24126447436439 24.075031365654343 14 23.16706965766975 22.046207064104493 26.228119902356628 28.55860337586913 15 24.132446199747594 28.72219165250921 23.547621108127156 23.597741039616036 16 23.637133613807084 28.183724191555243 24.227840953795884 23.95130124084179 17 23.508731818642538 26.516977906298116 25.234268587251773 24.740021687807573 18 24.99330108908788 24.75783263505768 27.33803560627342 22.91083066958102 19 26.132792795294357 25.508443592911917 26.215770937750193 22.14299267404353 20 25.314662400047393 25.424305382317996 25.955673503655614 23.305358713978993 21 25.44256644339532 25.785271681254972 26.02227283224475 22.74988904310496 22 24.18070911876189 25.738113860721707 26.273076905276504 23.808100115239895 23 23.02482223712547 25.402075253991597 27.207636016426896 24.365466492456044 24 23.23178885190724 26.601238306694047 26.79295043672134 23.37402240467737 25 23.25417052129723 26.13483486137908 26.893805641466834 23.717188975856864 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10179.0 1 10179.0 2 9568.0 3 8957.0 4 8957.0 5 8957.0 6 9493.5 7 10030.0 8 10030.0 9 10030.0 10 11159.5 11 12289.0 12 12289.0 13 12289.0 14 13300.0 15 14311.0 16 14311.0 17 14311.0 18 22897.0 19 31483.0 20 31483.0 21 31483.0 22 44421.5 23 57360.0 24 57360.0 25 57360.0 26 76539.0 27 95718.0 28 95718.0 29 95718.0 30 111299.0 31 126880.0 32 126880.0 33 126880.0 34 143700.0 35 160520.0 36 160520.0 37 160520.0 38 172912.0 39 185304.0 40 185304.0 41 185304.0 42 203175.0 43 221046.0 44 221046.0 45 221046.0 46 232832.0 47 244618.0 48 244618.0 49 244618.0 50 249269.0 51 253920.0 52 253920.0 53 253920.0 54 235877.0 55 217834.0 56 217834.0 57 217834.0 58 198421.5 59 179009.0 60 179009.0 61 179009.0 62 157187.5 63 135366.0 64 135366.0 65 135366.0 66 111631.0 67 87896.0 68 87896.0 69 87896.0 70 68467.0 71 49038.0 72 49038.0 73 49038.0 74 40186.0 75 31334.0 76 31334.0 77 31334.0 78 26745.0 79 22156.0 80 22156.0 81 22156.0 82 16781.0 83 11406.0 84 11406.0 85 11406.0 86 8627.0 87 5848.0 88 5848.0 89 5848.0 90 4169.5 91 2491.0 92 2491.0 93 2491.0 94 1349.5 95 208.0 96 208.0 97 208.0 98 133.0 99 58.0 100 58.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22778896673913313 2 0.6435555490173814 3 0.6397399114312365 4 0.6487503327190004 5 0.6653920291790542 6 0.6781721165157805 7 0.673528991260351 8 0.6692536383023815 9 0.6620361069647338 10 0.6634612246173904 11 0.6682882360215496 12 0.6689778090792866 13 0.6645645415097696 14 0.6589560139735084 15 0.6639209399892151 16 0.6725176174423367 17 0.6710924997896802 18 0.6647484276584995 19 0.6642427407494923 20 0.6716441582358699 21 0.667047004517623 22 0.6688398944677393 23 0.6737588489462634 24 0.6770687996234012 25 0.6637370538404852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2175259.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.37951525596065 #Duplication Level Percentage of deduplicated Percentage of total 1 87.82427124876905 45.12368484469755 2 7.552311701224462 7.760682285416648 3 1.6480598971224638 2.540295558808217 4 0.6996683141933577 1.4379447529283955 5 0.4013085486780188 1.0309519349574847 6 0.2768898210662177 0.8535878871415168 7 0.20038134354731632 0.7206847408459462 8 0.15005302315669028 0.6167721273985748 9 0.11815675303084232 0.5463753025448643 >10 0.8517783343757357 8.883062350302225 >50 0.13111078797428513 4.753278800160419 >100 0.12498306899927825 13.6173703619442 >500 0.014170475950675204 4.944972337704755 >1k 0.006490992209664126 5.493375096626265 >5k 2.7426727646468136E-4 1.0067594288620743 >10k+ 9.142242548822712E-5 0.6702021896609937 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 14268 0.6559218925194654 No Hit TATCAACGCAGAGTACTTTTTTTTT 9150 0.42063956521958995 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 6673 0.30676806761861464 No Hit GGTATCAACGCAGAGTACTTTTTTT 5610 0.2579003235936502 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 4829 0.22199655305414207 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4682 0.21523873708831914 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4643 0.21344584713820286 No Hit GNNNNNNNNNNNNNNNNNNNNNNNN 3706 0.17037051679822954 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 2420 0.11125111998157461 No Hit GTACAAAGGGCAGGGACTTAATCAA 2415 0.11102126229566227 No Hit GAATAGGACCGCGGTTCTATTTTGT 2390 0.10987197386610054 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2383 0.10955017310582327 No Hit GTATCTGATCGTCTTCGAACCTCCG 2334 0.10729756778388229 No Hit CTATTGGAGCTGGAATTACCGCGGC 2322 0.10674590933769267 No Hit GTCCTATTCCATTATTCCTAGCTGC 2269 0.10430941786702182 No Hit GATTAAGAGGGACGGCCGGGGGCAT 2221 0.10210278408226332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.19430743649377E-5 2 0.0 0.0 0.0 0.0 9.19430743649377E-5 3 0.0 0.0 0.0 0.0 9.19430743649377E-5 4 0.0 0.0 0.0 0.0 9.19430743649377E-5 5 0.0 0.0 0.0 0.0 9.19430743649377E-5 6 1.838861487298754E-4 0.0 0.0 0.0 9.19430743649377E-5 7 1.838861487298754E-4 0.0 0.0 0.0 9.19430743649377E-5 8 1.838861487298754E-4 0.0 0.0 0.0 9.19430743649377E-5 9 1.838861487298754E-4 0.0 0.0 0.0 9.19430743649377E-5 10 1.838861487298754E-4 0.0 0.0 0.0 9.19430743649377E-5 11 1.838861487298754E-4 0.0 0.0 9.19430743649377E-5 9.19430743649377E-5 12 1.838861487298754E-4 0.0 0.0 1.3791461154740654E-4 1.3791461154740654E-4 13 1.838861487298754E-4 0.0 0.0 1.838861487298754E-4 1.838861487298754E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 50 8.7126464E-5 15.200778 1 CGCAGAT 155 0.0 12.870136 8 AACGGAC 60 4.093007E-4 12.666728 15 ACTCTAA 360 0.0 11.874233 10 GTATCAA 7860 0.0 11.809128 1 GTTCTAA 145 9.822543E-11 11.793708 1 TAAGGGT 250 0.0 11.78033 4 GCGTTAT 155 2.5465852E-11 11.645757 1 CTCGTAG 200 0.0 11.399263 10 CGGTCCA 410 0.0 11.352925 10 GTATAGA 135 4.7275535E-9 11.259836 1 CGAGACT 135 4.7293724E-9 11.259575 4 GCTCGTA 195 0.0 11.204406 9 AGGCCCG 520 0.0 11.143511 10 CGAGTGT 60 0.0058797416 11.082618 8 CGAACGA 190 0.0 11.000308 16 CTTACTC 95 1.3603423E-5 11.000308 3 CACTCTA 435 0.0 10.918836 9 ACCGGTC 105 3.4703335E-6 10.8564415 8 CTGTCGA 185 5.456968E-12 10.783088 9 >>END_MODULE