FastQCFastQC Report
Thu 2 Feb 2017
SRR4050263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175259
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT217631.0004785637020694No Hit
TATCAACGCAGAGTACTTTTTTTTT165600.7612886557416841No Hit
GTACTTTTTTTTTTTTTTTTTTTTT120570.5542788238090268No Hit
ACGCAGAGTACTTTTTTTTTTTTTT96270.44256798845562756No Hit
GGTATCAACGCAGAGTACTTTTTTT89060.40942251014706754No Hit
GAGTACTTTTTTTTTTTTTTTTTTT81350.37397845497938403No Hit
GCAGAGTACTTTTTTTTTTTTTTTT47190.21693968396407048No Hit
ATCAACGCAGAGTACTTTTTTTTTT26930.12380134963238862No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25000.1149288429561721No Hit
GAATAGGACCGCGGTTCTATTTTGT24780.1139174691381578No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA24760.11382552606379287No Hit
GATTAAGAGGGACGGCCGGGGGCAT24360.1119866645764941No Hit
CTATTGGAGCTGGAATTACCGCGGC24030.11046960384947263No Hit
GTACAAAGGGCAGGGACTTAATCAA23750.1091824008083635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG250.00602402719.0048085
GGTATCA25850.015.4084751
TTGACGT400.005271145614.2536064
AACCGCG2600.014.2463967
ACCGAGT500.001502744713.2966378
ACGACTA905.391721E-712.6698725
GTATCAA75750.012.6171771
CGACCAT3300.012.08812910
TAACGCT1650.012.086184519
AACCGTG1603.6379788E-1211.8719977
ACCGTGT2000.011.8719978
CGCGGTC2900.011.79039110
CGGTCCA3050.011.5219410
CGGTTCT5400.011.43255612
GAACCGC3250.011.3968546
GATACGC1501.7826096E-1011.39633116
GTTAGGT1701.0913936E-1111.1792984
GACCATA3400.011.17390111
AGAACCG3500.011.1313885
TAATACG1201.2715645E-711.0861384
Produced by FastQC (version 0.11.5)