##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2175259
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.432833055741867	31.0	30.0	34.0	26.0	34.0
2	30.383621904334152	31.0	30.0	34.0	26.0	34.0
3	30.57282236276232	31.0	31.0	34.0	26.0	34.0
4	34.16155271625126	37.0	35.0	37.0	30.0	37.0
5	34.101674329355724	37.0	35.0	37.0	30.0	37.0
6	34.04374467592135	37.0	35.0	37.0	30.0	37.0
7	33.951443483281764	37.0	35.0	37.0	30.0	37.0
8	33.901381398720794	37.0	35.0	37.0	30.0	37.0
9	35.433593424966865	39.0	35.0	39.0	30.0	39.0
10	35.32965085996656	39.0	35.0	39.0	30.0	39.0
11	35.30223941149077	39.0	35.0	39.0	30.0	39.0
12	35.24464121283948	39.0	35.0	39.0	29.0	39.0
13	35.21131092895145	39.0	35.0	39.0	29.0	39.0
14	36.3193693256757	40.0	37.0	41.0	29.0	41.0
15	36.275595227970555	40.0	36.0	41.0	29.0	41.0
16	36.28091827226091	40.0	36.0	41.0	29.0	41.0
17	36.28443969200909	40.0	36.0	41.0	29.0	41.0
18	36.241056352369995	40.0	36.0	41.0	29.0	41.0
19	36.28208916731295	40.0	37.0	41.0	29.0	41.0
20	36.23249737157736	40.0	37.0	41.0	29.0	41.0
21	36.16388393290178	40.0	37.0	41.0	29.0	41.0
22	36.09189848197387	40.0	36.0	41.0	29.0	41.0
23	35.96494348489077	40.0	36.0	41.0	28.0	41.0
24	35.90051667410639	40.0	36.0	41.0	28.0	41.0
25	35.68155975909076	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9397462792985074
1101	2	1.14852458975054
1101	3	1.1474585078443695
1101	4	1.112366409853074
1101	5	1.1198631435131432
1101	6	1.238561678181405
1101	7	1.160217518085389
1101	8	1.095944140521702
1101	9	1.2836054885105028
1101	10	1.3366207945049382
1101	11	1.35210030650412
1101	12	1.311120582434313
1101	13	1.323945244675258
1101	14	1.553183929228382
1101	15	1.5023502575162908
1101	16	1.5094182111850714
1101	17	1.5941825727284353
1101	18	1.5658825418067863
1101	19	1.5137487509635221
1101	20	1.5244194704439522
1101	21	1.5080352136325246
1101	22	1.5278996826643407
1101	23	1.6687635041450974
1101	24	1.674292286189356
1101	25	1.7020044455085568
1102	1	0.7382683963791905
1102	2	0.9672902432252464
1102	3	0.8446281322259459
1102	4	0.760115720369285
1102	5	0.8750306174199594
1102	6	0.9478025475969147
1102	7	0.9120041663008465
1102	8	1.0152258104855463
1102	9	1.1718230307972206
1102	10	1.0931193213322743
1102	11	1.194239379201214
1102	12	1.139909332397771
1102	13	1.1520867407170172
1102	14	1.2285848693301915
1102	15	1.3436098120236792
1102	16	1.315978621009691
1102	17	1.2389035145194356
1102	18	1.3020417029267222
1102	19	1.2022003441664637
1102	20	1.2295778486871214
1102	21	1.2719832514055156
1102	22	1.2201373458584186
1102	23	1.2963496774369787
1102	24	1.3370072118485439
1102	25	1.433589787196901
1103	1	0.6330140596750731
1103	2	0.8204027347682832
1103	3	0.71333304580358
1103	4	0.6444800092226899
1103	5	0.7464869799315821
1103	6	0.7562657885113566
1103	7	0.7593372760176749
1103	8	0.8637713226646682
1103	9	0.8699157195471869
1103	10	0.8991817946333001
1103	11	0.8830263357439492
1103	12	0.8723534242461994
1103	13	0.8773577257287144
1103	14	0.8605109626107605
1103	15	0.9068220134067317
1103	16	0.972442089345634
1103	17	0.9669860966227688
1103	18	1.0398293219772654
1103	19	1.0793924042584209
1103	20	1.0432319414619258
1103	21	1.0353863024537446
1103	22	0.9976584207630665
1103	23	1.1045409345290835
1103	24	1.191821632830326
1103	25	1.1806591011435046
1104	1	0.7604129854809507
1104	2	0.8969413372424206
1104	3	0.819759152691045
1104	4	0.7472850211539495
1104	5	0.8330921971445449
1104	6	0.920055590820418
1104	7	0.8611041836416362
1104	8	0.8751341841358595
1104	9	1.0497714417408304
1104	10	0.9800683008332598
1104	11	0.935313961124983
1104	12	1.101431226096615
1104	13	0.9894531498722756
1104	14	1.1278343608719936
1104	15	1.1879020428842608
1104	16	1.077184840289675
1104	17	1.0929959404956833
1104	18	1.1094349415457145
1104	19	1.0083100888837748
1104	20	1.1365626460309741
1104	21	1.1780262828466093
1104	22	1.1976862191099755
1104	23	1.2804756487225006
1104	24	1.283551582854919
1104	25	1.3293816146403756
1105	1	0.5752938214040952
1105	2	0.71460864919068
1105	3	0.6410382063392106
1105	4	0.5700319347645362
1105	5	0.6141370420852468
1105	6	0.718449891630442
1105	7	0.6263843305652728
1105	8	0.6744889353124606
1105	9	0.719959038766568
1105	10	0.7342422633767782
1105	11	0.8490152000066615
1105	12	0.7608644138892942
1105	13	0.7978623259142097
1105	14	0.9173692960374211
1105	15	0.9694262773210625
1105	16	0.9486684406660331
1105	17	0.9838309099734417
1105	18	0.9792501170351073
1105	19	0.8940311443665223
1105	20	0.9511490251163792
1105	21	0.9177557364939943
1105	22	0.9779380460696387
1105	23	0.9438697971425114
1105	24	1.019530865678007
1105	25	1.0457744544725713
1106	1	0.586015040974587
1106	2	0.7110568277425742
1106	3	0.614903054785696
1106	4	0.5783767075547246
1106	5	0.5940945468255734
1106	6	0.6525990558494001
1106	7	0.7525150417515079
1106	8	0.6712862967895319
1106	9	0.8111399024841361
1106	10	0.7324527953611195
1106	11	0.8242052618978235
1106	12	0.7819997118804523
1106	13	0.7393738080426715
1106	14	0.7477190174752266
1106	15	0.8445941373758004
1106	16	0.859028289920289
1106	17	0.7952108155164339
1106	18	0.7230772979403923
1106	19	0.7832265970525185
1106	20	0.8423029632025418
1106	21	0.9452544587074527
1106	22	0.8757240033608653
1106	23	0.9171980224788641
1106	24	0.9147909153156419
1106	25	0.8726459511881117
1107	1	0.2980059253873115
1107	2	0.42834316699578423
1107	3	0.4771507447606531
1107	4	0.3806291111015696
1107	5	0.4230087084396601
1107	6	0.37089393127525483
1107	7	0.3309448189465556
1107	8	0.33842666707003843
1107	9	0.3471492696569456
1107	10	0.3710083141255396
1107	11	0.37068294565810334
1107	12	0.3937004867309639
1107	13	0.4334447087206925
1107	14	0.31750730715621955
1107	15	0.4794202115097903
1107	16	0.4667638501268385
1107	17	0.39365288676011545
1107	18	0.48366313385799486
1107	19	0.4054693723334353
1107	20	0.49813624610297325
1107	21	0.44228461587709944
1107	22	0.43600195783155726
1107	23	0.41132662693065214
1107	24	0.4070278745096019
1107	25	0.45766622704360316
1108	1	0.3906188988034742
1108	2	0.5041167331260112
1108	3	0.38691023936294755
1108	4	0.43226103693172035
1108	5	0.5310273069631251
1108	6	0.4933552923872355
1108	7	0.5021325245797215
1108	8	0.5355312469739673
1108	9	0.437726496626901
1108	10	0.5009949293554499
1108	11	0.5008727851459795
1108	12	0.4935785521761886
1108	13	0.46282620620316806
1108	14	0.5105237508017808
1108	15	0.5478060502828725
1108	16	0.6098999508139471
1108	17	0.6525099605410318
1108	18	0.7273409323085858
1108	19	0.5744577009540706
1108	20	0.5510630258043747
1108	21	0.6218837034702602
1108	22	0.5510030615495154
1108	23	0.7299551084600182
1108	24	0.7391387088152612
1108	25	0.6972364427766635
1109	1	0.041299088652021254
1109	2	0.165797692176902
1109	3	0.22352007238893634
1109	4	0.162642850166705
1109	5	0.1513853051560048
1109	6	0.24688253860924902
1109	7	0.2889116493013475
1109	8	0.24222481892797276
1109	9	0.26772152536282334
1109	10	0.18873925186956342
1109	11	0.2378040708559439
1109	12	0.1924983099278279
1109	13	0.18136011996516999
1109	14	0.2921179814482784
1109	15	0.45224441500570833
1109	16	0.4777530029349677
1109	17	0.27378881822516377
1109	18	0.3742448088868784
1109	19	0.4319658212452282
1109	20	0.44482157016450685
1109	21	0.2847040225044921
1109	22	0.3451715672479949
1109	23	0.4772410669902669
1109	24	0.4769552490170028
1109	25	0.36295453851380444
1110	1	-0.12303241698599976
1110	2	0.018352844237121246
1110	3	-0.09921914660196407
1110	4	-0.08237712096301664
1110	5	0.07210917830816044
1110	6	-0.05704675439638862
1110	7	-0.021058264893959233
1110	8	-0.007341560043577999
1110	9	0.0996965258666691
1110	10	0.09713893520340378
1110	11	0.09171708339159323
1110	12	0.12756978024636112
1110	13	0.07702576760667768
1110	14	0.1364192186380393
1110	15	0.21447271694954395
1110	16	0.18488167596014904
1110	17	0.14302531141822783
1110	18	0.12679268669455013
1110	19	0.1413802155739532
1110	20	0.20831814276343863
1110	21	0.04353940522464228
1110	22	0.1991938510231961
1110	23	0.1712014534892603
1110	24	0.17356853337666678
1110	25	0.33935998078835894
1111	1	0.055404466073607495
1111	2	0.07109096572800055
1111	3	0.018978538602667072
1111	4	0.23628506347853317
1111	5	0.1675870489624458
1111	6	0.05480803971178716
1111	7	0.14994339253172484
1111	8	0.2419334256048984
1111	9	0.15948877009456197
1111	10	0.1681336793962842
1111	11	0.07568341507275278
1111	12	0.24474557588069956
1111	13	0.15293765801705916
1111	14	0.27166589853610645
1111	15	0.17621716186070557
1111	16	0.19427382777823965
1111	17	0.0954826156424744
1111	18	0.001075548177865926
1111	19	-0.01133265949080453
1111	20	0.0852768191331279
1111	21	0.02928348784740109
1111	22	-0.03703547053488876
1111	23	0.02839090932157262
1111	24	0.06379252211947062
1111	25	0.023281070173837293
1112	1	-0.5994422289760166
1112	2	-0.4676423573723909
1112	3	-0.5989663460804167
1112	4	-0.48581652244354245
1112	5	-0.5911868147378527
1112	6	-1.4753441377722467
1112	7	-0.9284332345170085
1112	8	-0.6136939070138254
1112	9	-0.7514641366780808
1112	10	-0.7187088096582173
1112	11	-0.8094446306631227
1112	12	-0.8306893632169832
1112	13	-0.7761605120575936
1112	14	-0.9535974828452893
1112	15	-0.8691643859301905
1112	16	-0.8156646710942184
1112	17	-0.851701220047623
1112	18	-0.8644946769596586
1112	19	-0.9354772767850719
1112	20	-0.9496138350567165
1112	21	-0.8811063235418644
1112	22	-1.028600449091769
1112	23	-0.923298185492321
1112	24	-0.9481291411506234
1112	25	-1.0358647794673672
1113	1	-0.7492908834200804
1113	2	-0.8547141002148955
1113	3	-0.7421915804377193
1113	4	-0.6184408083079944
1113	5	-0.603825706807271
1113	6	-1.193938658872753
1113	7	-0.7627824817346109
1113	8	-0.7510973519703157
1113	9	-0.8049403941456887
1113	10	-0.803523984881636
1113	11	-0.9382792767799373
1113	12	-0.8653101247206152
1113	13	-0.9689185492639609
1113	14	-1.0896085928313397
1113	15	-1.0279091958853641
1113	16	-1.0254133886368706
1113	17	-1.0435129164338974
1113	18	-0.9694001435815522
1113	19	-1.0023421875986571
1113	20	-1.1386025112473064
1113	21	-1.0561051578596619
1113	22	-1.1079056031728811
1113	23	-1.0312668442294068
1113	24	-1.0796743777592823
1113	25	-1.10481564065131
1114	1	-0.8941737118622619
1114	2	-0.9867379975195902
1114	3	-1.0497238956434423
1114	4	-0.8689170739082925
1114	5	-0.9929431047172841
1114	6	-1.4656126019623201
1114	7	-1.0215737610017825
1114	8	-1.2150519992209894
1114	9	-1.3611747852754235
1114	10	-1.4933069954344518
1114	11	-1.3471334888170716
1114	12	-1.3237352621609801
1114	13	-1.540288700268313
1114	14	-1.6411171616338365
1114	15	-1.515555167330696
1114	16	-1.5897290333462308
1114	17	-1.5954498396216223
1114	18	-1.6742298821447648
1114	19	-1.5852758492569947
1114	20	-1.5948465051975234
1114	21	-1.4887216177798877
1114	22	-1.5051489077777802
1114	23	-1.517873790498193
1114	24	-1.7109856086007937
1114	25	-1.5506772708672685
1115	1	-1.616818533640192
1115	2	-2.069309724012097
1115	3	-1.820208465772719
1115	4	-1.5218505453415005
1115	5	-1.497550060626459
1115	6	-2.0924557993230835
1115	7	-1.702883966094035
1115	8	-1.6934549230324123
1115	9	-1.7448308049999923
1115	10	-1.6209099513296792
1115	11	-1.8348981192081126
1115	12	-1.6715194218267513
1115	13	-1.8149113842183837
1115	14	-1.8576700354014832
1115	15	-1.823933923664633
1115	16	-2.012410960746152
1115	17	-1.8920689036811353
1115	18	-2.001521904087305
1115	19	-2.149230666884961
1115	20	-2.0981775717400453
1115	21	-2.2887507024497538
1115	22	-2.393851571082621
1115	23	-2.281193418465513
1115	24	-2.2718892560415895
1115	25	-2.3334694689220115
1116	1	-1.4150527826262795
1116	2	-1.4942345919066682
1116	3	-1.5540928694777207
1116	4	-1.4514589486957519
1116	5	-1.4418200682275923
1116	6	-1.7430309016523253
1116	7	-1.7564433451926575
1116	8	-1.7895152294129062
1116	9	-1.913809697673031
1116	10	-1.8790978151207582
1116	11	-1.8813107458096354
1116	12	-1.9322373232202281
1116	13	-2.0845492448911784
1116	14	-2.24861348081987
1116	15	-2.2604886178210464
1116	16	-2.1937082831874974
1116	17	-2.203357780940898
1116	18	-2.1806842508630595
1116	19	-2.3392144283153797
1116	20	-2.371061036205802
1116	21	-2.52787336953282
1116	22	-2.357642327524239
1116	23	-2.4880790315055066
1116	24	-2.4772600351506213
1116	25	-2.743894508674387
1201	1	0.8893031093297914
1201	2	1.1075867092768164
1201	3	1.030168349722647
1201	4	0.7479071704218683
1201	5	0.9635933006251207
1201	6	1.0369176433297724
1201	7	0.9476289472005206
1201	8	0.9984012361928194
1201	9	1.1597049180062342
1201	10	1.1531197129158386
1201	11	1.1783536731201067
1201	12	1.2218006437178914
1201	13	1.2212403981075042
1201	14	1.382233868740947
1201	15	1.3606245840474571
1201	16	1.4322859320612977
1201	17	1.4120561724394989
1201	18	1.3869827043364182
1201	19	1.4059990776733358
1201	20	1.4186101524664636
1201	21	1.4573561135457282
1201	22	1.4532046199147004
1201	23	1.462848211795638
1201	24	1.5107365231835814
1201	25	1.502680511514619
1202	1	0.6216250752274846
1202	2	0.7760752988817572
1202	3	0.7839003324492246
1202	4	0.7433486858433653
1202	5	0.7446729221713255
1202	6	0.7543133126468504
1202	7	0.7350013063636709
1202	8	0.9065134919545201
1202	9	0.905137794849054
1202	10	0.9762414290885673
1202	11	0.9504487058906719
1202	12	0.9835379039156109
1202	13	0.9421774177324878
1202	14	1.0641401555716428
1202	15	1.0238086193937193
1202	16	1.1194889517348443
1202	17	1.0613672341161475
1202	18	1.1248949980144758
1202	19	1.2058207960874228
1202	20	1.1368804195059425
1202	21	1.2166874128439957
1202	22	1.22078961418989
1202	23	1.2832099020806425
1202	24	1.2863927516455078
1202	25	1.2584035698889977
1203	1	0.6056273102430332
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2211	10	-0.3674715650606828
2211	11	-0.36746761426589813
2211	12	-0.37484535543661934
2211	13	-0.34741960768060665
2211	14	-0.5256212632114199
2211	15	-0.5270694011610288
2211	16	-0.44669481790877796
2211	17	-0.43076770065834324
2211	18	-0.3884685262442247
2211	19	-0.38118350403834
2211	20	-0.40940856951155524
2211	21	-0.4559045425720214
2211	22	-0.5098922605637455
2211	23	-0.49637947630675683
2211	24	-0.5574324587293162
2211	25	-0.5690386156314915
2212	1	-0.40176017841491074
2212	2	-0.4428348507142559
2212	3	-0.42178053423234374
2212	4	-0.3699407668536878
2212	5	-0.5255270583010656
2212	6	-0.4365160808702555
2212	7	-0.5015329141579059
2212	8	-0.484038695581539
2212	9	-0.612113072798131
2212	10	-0.6195413604169815
2212	11	-0.506677567001482
2212	12	-0.49837498411829273
2212	13	-0.5381342601275847
2212	14	-0.4649477786520464
2212	15	-0.5570019270216093
2212	16	-0.709682929691148
2212	17	-0.583350507201132
2212	18	-0.654355868018861
2212	19	-0.5652166390928812
2212	20	-0.5904746142195307
2212	21	-0.504537941954645
2212	22	-0.4937288406113609
2212	23	-0.6363570583877802
2212	24	-0.6885602650877161
2212	25	-0.7300579261572011
2213	1	-0.6794336210311442
2213	2	-0.9866773965719098
2213	3	-0.7564521474959207
2213	4	-0.7491223578609905
2213	5	-0.7944122207308411
2213	6	-0.7372449608999077
2213	7	-0.71613767320774
2213	8	-0.76755569011398
2213	9	-0.8675571477281423
2213	10	-0.9375000607738002
2213	11	-0.8654027763692724
2213	12	-0.9537870752682238
2213	13	-0.9914089038650005
2213	14	-1.1203325832392679
2213	15	-1.2114547609809563
2213	16	-1.1498990956344457
2213	17	-1.1420676458954375
2213	18	-1.3126104589083099
2213	19	-1.1350508102389867
2213	20	-1.258841246553807
2213	21	-1.2126257389255386
2213	22	-1.325835132662128
2213	23	-1.2849441354033573
2213	24	-1.3131827796192042
2213	25	-1.362637099891046
2214	1	-0.9643897011277254
2214	2	-1.0092372400422036
2214	3	-0.9806817799008982
2214	4	-0.9580795203438228
2214	5	-1.081466673085032
2214	6	-0.927852137953316
2214	7	-0.9970418820250231
2214	8	-0.9514966168031265
2214	9	-1.190140720435359
2214	10	-1.119152989461199
2214	11	-1.247042591531006
2214	12	-1.163146594022642
2214	13	-1.0385876224862045
2214	14	-1.277711543315867
2214	15	-1.3484409397412023
2214	16	-1.350960042306106
2214	17	-1.3727497573812215
2214	18	-1.3243549741241623
2214	19	-1.2205474590046492
2214	20	-1.3279703419286903
2214	21	-1.4680853367774205
2214	22	-1.4241672170882609
2214	23	-1.5516719985231262
2214	24	-1.4703900149549511
2214	25	-1.417073842741054
2215	1	-1.925159977918529
2215	2	-3.629406560566835
2215	3	-2.1627671420099475
2215	4	-1.9303811389778502
2215	5	-1.781774608339667
2215	6	-1.849487440844051
2215	7	-1.9243481065282495
2215	8	-1.9578974590192075
2215	9	-2.1749211740038135
2215	10	-2.144082585460673
2215	11	-2.148166423614178
2215	12	-2.05883637776612
2215	13	-2.2823039009438943
2215	14	-2.662332326752285
2215	15	-2.5451422600903726
2215	16	-2.6236327624008524
2215	17	-2.5414438838013638
2215	18	-2.444235660179345
2215	19	-2.4768425855563905
2215	20	-2.553450887614524
2215	21	-2.5297776647153043
2215	22	-2.57058648278111
2215	23	-2.7545776440027936
2215	24	-2.8313287349619642
2215	25	-2.758202938674728
2216	1	-1.7908585469379332
2216	2	-2.515145024975048
2216	3	-1.915296425910558
2216	4	-1.7495492744394454
2216	5	-1.8542984104311344
2216	6	-1.8689904234811792
2216	7	-2.207380765167617
2216	8	-2.1376937184458242
2216	9	-2.338532239644671
2216	10	-2.4702192423945917
2216	11	-2.2802762729786323
2216	12	-2.315015252244109
2216	13	-2.5169685017806316
2216	14	-2.677671875340529
2216	15	-2.818418288215099
2216	16	-2.7049335500302405
2216	17	-2.6459043253154135
2216	18	-3.0165475751199438
2216	19	-2.760320869597855
2216	20	-2.852569306112585
2216	21	-2.832859507364546
2216	22	-2.7613711809199586
2216	23	-2.9858643844962316
2216	24	-2.8816223543645236
2216	25	-2.961450073097062
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44298.0
3	3.0
4	746.0
5	5828.0
6	7711.0
7	6540.0
8	4907.0
9	3923.0
10	3929.0
11	3972.0
12	3426.0
13	2997.0
14	2766.0
15	2999.0
16	2990.0
17	3175.0
18	3865.0
19	5575.0
20	7401.0
21	8947.0
22	10100.0
23	11630.0
24	13078.0
25	14474.0
26	16415.0
27	18318.0
28	21270.0
29	25442.0
30	32496.0
31	41746.0
32	54153.0
33	71913.0
34	97763.0
35	133659.0
36	195374.0
37	342252.0
38	949178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.67706664809527	19.145202330587136	13.466921316164632	24.71080970515296
2	16.10451660243802	22.462738650563992	36.86094337488655	24.571801372111434
3	17.77232044551936	26.91555350420341	29.47350177611034	25.838624274166893
4	12.408959117052268	17.721016210023727	37.17060818964546	32.69941648327854
5	14.366853922765369	37.0830546875503	32.96688538252585	15.583206007158475
6	30.43132466534204	39.00283467404926	16.15033242828495	14.415508232323743
7	26.819775412309564	32.63730090208378	22.03774757652909	18.505176109077567
8	26.186057437916414	33.02084860933418	20.18882401265508	20.604269940094326
9	26.833999378398808	14.98003703860364	19.942593842470075	38.24336974052748
10	16.014435480125925	27.132596662220525	30.875126843804296	25.97784101384925
11	34.64836079780682	23.574085576243625	23.134844028792987	18.64270959715656
12	24.08736035295032	25.65023031792103	29.079781171538695	21.182628157589964
13	28.81745901835323	21.59672551446611	25.77346938400166	23.812346083178994
14	23.086679925229795	21.532614502220028	26.916905955227982	28.463799617322195
15	23.923629120363728	28.182074003774126	24.431582006319637	23.462714869542506
16	23.476800806984425	27.477792066338974	25.03826417429613	24.00714295238047
17	23.359414617012465	26.007402799749713	26.27800818620372	24.355174397034098
18	24.64096179943878	24.154950038064484	28.44593923657721	22.758148925919524
19	25.5244662011692	24.834954973510044	27.831142847160027	21.809435978160728
20	24.677612590785674	24.687083080097352	27.56946787803113	23.065836451085843
21	25.01156210204992	24.90867548420612	27.58023363261478	22.49952878112918
22	23.74086200349568	24.849466132293273	27.889887084658465	23.519784779552584
23	22.687326336849964	24.39508676951142	28.78399476275154	24.133592130887077
24	22.998584480406844	25.66287830898968	28.219364620653163	23.119172589950317
25	23.03008765586419	24.993805198668085	28.465008681916377	23.511098463551352
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4401.0
1	4401.0
2	4742.0
3	5083.0
4	5083.0
5	5083.0
6	10176.0
7	15269.0
8	15269.0
9	15269.0
10	15443.0
11	15617.0
12	15617.0
13	15617.0
14	14911.5
15	14206.0
16	14206.0
17	14206.0
18	23517.5
19	32829.0
20	32829.0
21	32829.0
22	47676.5
23	62524.0
24	62524.0
25	62524.0
26	83764.0
27	105004.0
28	105004.0
29	105004.0
30	118892.0
31	132780.0
32	132780.0
33	132780.0
34	147032.5
35	161285.0
36	161285.0
37	161285.0
38	172059.5
39	182834.0
40	182834.0
41	182834.0
42	199775.0
43	216716.0
44	216716.0
45	216716.0
46	228632.0
47	240548.0
48	240548.0
49	240548.0
50	244027.5
51	247507.0
52	247507.0
53	247507.0
54	230396.5
55	213286.0
56	213286.0
57	213286.0
58	196052.5
59	178819.0
60	178819.0
61	178819.0
62	157137.5
63	135456.0
64	135456.0
65	135456.0
66	111691.5
67	87927.0
68	87927.0
69	87927.0
70	68447.5
71	48968.0
72	48968.0
73	48968.0
74	40052.0
75	31136.0
76	31136.0
77	31136.0
78	26962.5
79	22789.0
80	22789.0
81	22789.0
82	17203.5
83	11618.0
84	11618.0
85	11618.0
86	8751.0
87	5884.0
88	5884.0
89	5884.0
90	4219.0
91	2554.0
92	2554.0
93	2554.0
94	1366.5
95	179.0
96	179.0
97	179.0
98	109.5
99	40.0
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3240993371364054
2	0.00997582356859574
3	0.0
4	0.0
5	0.048408028653139694
6	0.002436491470670849
7	0.003355922214320226
8	2.758292230948131E-4
9	0.010619425089150304
10	0.015630322642039406
11	0.006711844428640452
12	0.010205681254508084
13	0.017607098740885566
14	0.0028042637681306
15	0.02073316326929345
16	0.011400941221252275
17	0.006252129056815763
18	1.3791461154740654E-4
19	0.004367296032334541
20	0.0037236945117799765
21	0.0022526053219409734
22	4.597153718246885E-5
23	0.0032639791399552884
24	0.003953552197692321
25	0.0022066337847585047
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2175259.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.442705984983704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.71984199556006	44.24826198831263
2	7.557101251223863	7.624012730284756
3	1.6687835521345045	2.525338742186926
4	0.7174520262474153	1.447608864733167
5	0.4359075101221791	1.0994177184869693
6	0.27455935684759486	0.8309710147733669
7	0.19612601226910997	0.6925188741028613
8	0.16107709672649553	0.6500131704871516
9	0.11973640571032078	0.5435845478049991
>10	0.8729776130565685	8.958535930280451
>50	0.12926892222771832	4.609099633456804
>100	0.1251082556431197	13.075776715446677
>500	0.014890501506111724	5.0923427181220315
>1k	0.006618000669382989	5.030166906329354
>5k	2.7575002789095783E-4	1.2364687165452226
>10k+	2.7575002789095783E-4	2.3358817286466294
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	21763	1.0004785637020694	No Hit
TATCAACGCAGAGTACTTTTTTTTT	16560	0.7612886557416841	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	12057	0.5542788238090268	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	9627	0.44256798845562756	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8906	0.40942251014706754	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	8135	0.37397845497938403	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4719	0.21693968396407048	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2693	0.12380134963238862	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2500	0.1149288429561721	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2478	0.1139174691381578	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2476	0.11382552606379287	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2436	0.1119866645764941	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2403	0.11046960384947263	No Hit
GTACAAAGGGCAGGGACTTAATCAA	2375	0.1091824008083635	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.597153718246885E-5
2	0.0	0.0	0.0	0.0	4.597153718246885E-5
3	0.0	0.0	0.0	4.597153718246885E-5	4.597153718246885E-5
4	0.0	0.0	0.0	4.597153718246885E-5	4.597153718246885E-5
5	0.0	0.0	0.0	4.597153718246885E-5	4.597153718246885E-5
6	0.0	0.0	0.0	4.597153718246885E-5	4.597153718246885E-5
7	0.0	0.0	0.0	4.597153718246885E-5	4.597153718246885E-5
8	0.0	0.0	0.0	4.597153718246885E-5	4.597153718246885E-5
9	0.0	0.0	0.0	4.597153718246885E-5	4.597153718246885E-5
10	0.0	0.0	0.0	4.597153718246885E-5	4.597153718246885E-5
11	0.0	0.0	0.0	9.19430743649377E-5	4.597153718246885E-5
12	0.0	0.0	0.0	1.3791461154740654E-4	9.19430743649377E-5
13	4.597153718246885E-5	0.0	0.0	1.838861487298754E-4	9.19430743649377E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	25	0.006024027	19.004808	5
GGTATCA	2585	0.0	15.408475	1
TTGACGT	40	0.0052711456	14.253606	4
AACCGCG	260	0.0	14.246396	7
ACCGAGT	50	0.0015027447	13.296637	8
ACGACTA	90	5.391721E-7	12.669872	5
GTATCAA	7575	0.0	12.617177	1
CGACCAT	330	0.0	12.088129	10
TAACGCT	165	0.0	12.0861845	19
AACCGTG	160	3.6379788E-12	11.871997	7
ACCGTGT	200	0.0	11.871997	8
CGCGGTC	290	0.0	11.790391	10
CGGTCCA	305	0.0	11.52194	10
CGGTTCT	540	0.0	11.432556	12
GAACCGC	325	0.0	11.396854	6
GATACGC	150	1.7826096E-10	11.396331	16
GTTAGGT	170	1.0913936E-11	11.179298	4
GACCATA	340	0.0	11.173901	11
AGAACCG	350	0.0	11.131388	5
TAATACG	120	1.2715645E-7	11.086138	4
>>END_MODULE