FastQCFastQC Report
Thu 2 Feb 2017
SRR4050262_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050262_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2150854
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT133110.6188704579669284No Hit
TATCAACGCAGAGTACTTTTTTTTT85710.3984928777127597No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN68600.31894308028345947No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63260.29411573263457214No Hit
GGTATCAACGCAGAGTACTTTTTTT54200.2519929293201677No Hit
GAGTACTTTTTTTTTTTTTTTTTTT43760.20345406987178116No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42450.19736346586053727No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN32630.15170718235640357No Hit
CTATTGGAGCTGGAATTACCGCGGC24090.11200202338234022No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG23770.11051424224982262No Hit
GTATCTGATCGTCTTCGAACCTCCG23690.11014229696669324No Hit
GAATAGGACCGCGGTTCTATTTTGT23630.1098633380043462No Hit
GTACAAAGGGCAGGGACTTAATCAA23290.10828257055104623No Hit
GATTAAGAGGGACGGCCGGGGGCAT22700.1055394740879669No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22590.105028049323664No Hit
GTCCTATTCCATTATTCCTAGCTGC22160.10302884342684349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG602.569924E-514.2495435
TTACCGG1051.373337E-913.57067511
GTAACAC1001.4440047E-712.3496043
CCGGTAG701.0920731E-412.2133229
GCTCGTA1650.012.0899569
TTAGAAC1603.6379788E-1211.87461953
CTCGTAG1850.011.80987810
CGGTAGC658.030893E-411.69138410
GTATCAA80900.011.681911
CGGTCCA4400.011.4422810
ATCGATG1001.9323343E-611.3991018
CGTCAAT2850.011.33270519
CGACCAT3450.011.28896510
GTAAGGC855.3265278E-511.1761133
GGTCCAA4800.011.08271911
GGTATCA31550.011.0596841
GTATTAT1900.011.0089291
CCGTCGT3600.010.8185919
CGCCAGT2200.010.79485518
CCGACCA3700.010.7829329
Produced by FastQC (version 0.11.5)