##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050262_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2150854
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.76199965223116	31.0	30.0	34.0	25.0	34.0
2	29.77614938066461	31.0	30.0	34.0	25.0	34.0
3	29.92305428448421	31.0	30.0	34.0	25.0	34.0
4	33.27953268794628	37.0	35.0	37.0	27.0	37.0
5	33.36902039840919	37.0	35.0	37.0	27.0	37.0
6	33.40529808159921	37.0	35.0	37.0	28.0	37.0
7	33.23270012748424	37.0	35.0	37.0	27.0	37.0
8	33.15447863964732	37.0	35.0	37.0	26.0	37.0
9	34.55409990636277	39.0	35.0	39.0	26.0	39.0
10	34.47071023881677	39.0	35.0	39.0	26.0	39.0
11	34.50421692964748	39.0	35.0	39.0	26.0	39.0
12	34.425568634598164	39.0	35.0	39.0	25.0	39.0
13	34.39531507020002	39.0	35.0	39.0	25.0	39.0
14	35.43817990435427	40.0	36.0	41.0	25.0	41.0
15	35.418172037711535	40.0	36.0	41.0	25.0	41.0
16	35.45137187368366	40.0	36.0	41.0	25.0	41.0
17	35.43902003576254	40.0	36.0	41.0	25.0	41.0
18	35.369405361777225	40.0	36.0	41.0	25.0	41.0
19	35.40725265406206	40.0	36.0	41.0	25.0	41.0
20	35.389464835828	40.0	36.0	41.0	25.0	41.0
21	35.3151204126361	40.0	36.0	41.0	25.0	41.0
22	35.230905026561544	40.0	36.0	41.0	25.0	41.0
23	35.1441599476301	40.0	36.0	41.0	24.0	41.0
24	35.0963561450475	40.0	36.0	41.0	24.0	41.0
25	34.77712992141726	39.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.350998138231457
1101	2	1.4687046935751198
1101	3	1.4428722492961832
1101	4	1.5692602536071973
1101	5	1.5063385317563913
1101	6	1.4304844678043622
1101	7	1.4752190681027244
1101	8	1.5726880405977823
1101	9	1.7040975942287417
1101	10	1.742526462923955
1101	11	1.6170070776135717
1101	12	1.6547944106465025
1101	13	1.6816602827453337
1101	14	1.9055172228713744
1101	15	1.8841847081447156
1101	16	1.889582932858751
1101	17	1.8814303928443223
1101	18	1.9231533970481962
1101	19	1.9123081509397792
1101	20	1.883040615663738
1101	21	1.965725995356138
1101	22	2.0431212668122996
1101	23	2.184241973644866
1101	24	2.1357631552257956
1101	25	2.199454849135975
1102	1	1.0377814995464547
1102	2	0.9976654121684803
1102	3	1.068669120602756
1102	4	1.2229686826539776
1102	5	1.1137202359912166
1102	6	0.9473940799603255
1102	7	0.9456080031226293
1102	8	1.0518624097981473
1102	9	1.1366840090806463
1102	10	1.242911943168096
1102	11	1.113656551214845
1102	12	1.237956318410049
1102	13	1.1954915078573052
1102	14	1.3524330778677651
1102	15	1.3499181812853536
1102	16	1.2898126451426037
1102	17	1.2846281817878307
1102	18	1.4492302870226794
1102	19	1.4476462643897676
1102	20	1.378853669867354
1102	21	1.470437824629606
1102	22	1.4339600500039111
1102	23	1.4761596166392437
1102	24	1.4731353436747199
1102	25	1.4045303390171213
1103	1	0.8467187254204909
1103	2	0.9330476631959144
1103	3	0.9542211714390909
1103	4	0.9286917996028805
1103	5	0.925298856180703
1103	6	0.887625122818946
1103	7	0.8613326166122164
1103	8	0.8045435200011042
1103	9	0.8615133458828836
1103	10	1.0671950426189767
1103	11	1.092115574779605
1103	12	1.0220567247784018
1103	13	1.0232816928527626
1103	14	1.2459789583517207
1103	15	1.2478126636549476
1103	16	1.2813335909563222
1103	17	1.2234881631386259
1103	18	1.2520800458493895
1103	19	1.2424762919030954
1103	20	1.241288771804797
1103	21	1.2088379075149334
1103	22	1.3127788189265175
1103	23	1.254676199461258
1103	24	1.2276738911093048
1103	25	1.2768305220555547
1104	1	0.9343654833068307
1104	2	1.0179669355195884
1104	3	0.9654692854810243
1104	4	1.164476011315763
1104	5	1.0529782172961504
1104	6	0.9510405712000605
1104	7	0.9006593946154453
1104	8	0.8950205156482269
1104	9	1.0915659792873171
1104	10	1.1372213159658386
1104	11	1.1555579034825882
1104	12	1.235000033312474
1104	13	1.1623244932620338
1104	14	1.263672279892397
1104	15	1.1838544061934897
1104	16	1.3290422602936758
1104	17	1.261835977161482
1104	18	1.2582946028443445
1104	19	1.4456407994729403
1104	20	1.3191003667617025
1104	21	1.387190143784295
1104	22	1.4598011729490281
1104	23	1.4757187223962447
1104	24	1.4558574750366091
1104	25	1.3621941867972467
1105	1	0.4875928636844371
1105	2	0.6187987023716204
1105	3	0.5578706461752923
1105	4	0.7259264994779002
1105	5	0.574796932724297
1105	6	0.5184894369205466
1105	7	0.4919983256175584
1105	8	0.53049490418951
1105	9	0.6876890095443002
1105	10	0.729986929515924
1105	11	0.6878599937854304
1105	12	0.6764765369098669
1105	13	0.6573512725356281
1105	14	0.8318082937672955
1105	15	0.8304499950474948
1105	16	0.8062777332729993
1105	17	0.7849958007352527
1105	18	0.8199498364440814
1105	19	0.9068703872208062
1105	20	0.9145380869779345
1105	21	0.9728050867496663
1105	22	0.9465757409718947
1105	23	1.006424921577512
1105	24	0.9027137288302711
1105	25	0.8208669584520649
1106	1	0.6284719905842806
1106	2	0.8014654427396728
1106	3	0.754017428529739
1106	4	0.9366953053467952
1106	5	0.8096589751359318
1106	6	0.8478681449606
1106	7	0.7999397766906142
1106	8	0.828985322375658
1106	9	0.8322564228664504
1106	10	0.9707957768798678
1106	11	0.9197145127089001
1106	12	0.9971161713509886
1106	13	0.9944964103547846
1106	14	1.0349136178247846
1106	15	1.1803599984410766
1106	16	1.1952242216798012
1106	17	1.2061979541805599
1106	18	1.094252551628486
1106	19	1.170489131121066
1106	20	1.2011623870463026
1106	21	1.2165360575831272
1106	22	1.2712029518550523
1106	23	1.1924159259138989
1106	24	1.1680814183181454
1106	25	1.183287382158987
1107	1	0.2926552646569718
1107	2	0.3930969811113485
1107	3	0.41331972340291045
1107	4	0.4898389576349871
1107	5	0.35839033876602144
1107	6	0.287221026834132
1107	7	0.26006898565996295
1107	8	0.22036278855770774
1107	9	0.28135782364686435
1107	10	0.3213968840120742
1107	11	0.2717776110306218
1107	12	0.18639901279421167
1107	13	0.2150268229387322
1107	14	0.46032523516616664
1107	15	0.32138820229618403
1107	16	0.31562590648461253
1107	17	0.26595097014827473
1107	18	0.3276781099524584
1107	19	0.36836093584656027
1107	20	0.39885562509269334
1107	21	0.42414425747780626
1107	22	0.4668911680425367
1107	23	0.4555864637651936
1107	24	0.4001280659496018
1107	25	0.3221884809361555
1108	1	0.6098771980963313
1108	2	0.7248375201226089
1108	3	0.6019770502186681
1108	4	0.6948844095976625
1108	5	0.6967966227380984
1108	6	0.6183296696930398
1108	7	0.6030981947988323
1108	8	0.7085823538962401
1108	9	0.6562712535994919
1108	10	0.7128390739778396
1108	11	0.7181508464496389
1108	12	0.7357536616274842
1108	13	0.6624426961591041
1108	14	0.7236450360154834
1108	15	0.8313482526612148
1108	16	0.7363714798946859
1108	17	0.8569300624009983
1108	18	0.8379875972427513
1108	19	0.8128213723856064
1108	20	0.7764406600057328
1108	21	0.8031806588502661
1108	22	0.7864162376028077
1108	23	0.7958438758951658
1108	24	0.7458940277552912
1108	25	0.6855597869033687
1109	1	0.3303013134782695
1109	2	0.49010368067387233
1109	3	0.3574436695922749
1109	4	0.4360676699642241
1109	5	0.42552283178701344
1109	6	0.18473033634236202
1109	7	0.19218309521272658
1109	8	0.2218265780147135
1109	9	0.2965097308380109
1109	10	0.3466643383139285
1109	11	0.37363562256106775
1109	12	0.4380474042496658
1109	13	0.353441383530658
1109	14	0.3741774871856336
1109	15	0.418322542158009
1109	16	0.41719483610351205
1109	17	0.24141603513460552
1109	18	0.22316125861836156
1109	19	0.3845330166724352
1109	20	0.3760765058003699
1109	21	0.43756336843315324
1109	22	0.3751501311878016
1109	23	0.2229380701683965
1109	24	0.19278477868141408
1109	25	0.19945795238735542
1110	1	0.14976261028187565
1110	2	0.334834991119795
1110	3	0.2602663570187467
1110	4	0.26299986758164096
1110	5	0.3444214558359491
1110	6	0.1843296296871486
1110	7	0.0073942791936545404
1110	8	0.1630288048138695
1110	9	0.2449249202344248
1110	10	0.2663919743340557
1110	11	0.1749021178192791
1110	12	0.0776266193664128
1110	13	0.21213494548342027
1110	14	0.3423364833130833
1110	15	0.24287809850492437
1110	16	0.25124756913171353
1110	17	0.34878898902978506
1110	18	0.2974239939839478
1110	19	0.23382530931341705
1110	20	0.22005115970830502
1110	21	0.1853585510828708
1110	22	0.23111328721849134
1110	23	0.18054935994688748
1110	24	0.14940534937962724
1110	25	0.23016182668897045
1111	1	-0.4592336010611149
1111	2	-0.27330767728869176
1111	3	-0.45555850691839694
1111	4	-0.3807556941881103
1111	5	-0.2694509804723424
1111	6	-0.35342126771662663
1111	7	-0.5035781694115045
1111	8	-0.389432042928199
1111	9	-0.30384566052837414
1111	10	-0.381100749892866
1111	11	-0.3599457082398132
1111	12	-0.3133363053854197
1111	13	-0.2813237762046583
1111	14	-0.2578768534140181
1111	15	-0.31572500131632353
1111	16	-0.3782254546215995
1111	17	-0.24995400105754584
1111	18	-0.3378763583579101
1111	19	-0.3649681198007855
1111	20	-0.3862548194123505
1111	21	-0.24291809514254226
1111	22	-0.22753768067175173
1111	23	-0.32718194766264475
1111	24	-0.3141118159862657
1111	25	-0.36242307844079846
1112	1	-0.37743021498813434
1112	2	-0.29747621066001173
1112	3	-0.40412517060736164
1112	4	-0.43256946002689034
1112	5	-0.4399188028304408
1112	6	-0.473749140573986
1112	7	-0.4991437739316851
1112	8	-0.4486321754972167
1112	9	-0.565212225202643
1112	10	-0.5501361851741535
1112	11	-0.5467899547987685
1112	12	-0.5298306553814669
1112	13	-0.537695970794438
1112	14	-0.6486857790412941
1112	15	-0.703098066994059
1112	16	-0.635189044204509
1112	17	-0.6376684231335901
1112	18	-0.6778136147119298
1112	19	-0.6460128984901417
1112	20	-0.4893651288170844
1112	21	-0.5889838725116476
1112	22	-0.47985112634633964
1112	23	-0.5831673844965479
1112	24	-0.5737401046965189
1112	25	-0.6824490065159239
1113	1	-0.6565412916811226
1113	2	-0.6066173682544118
1113	3	-0.767303049649712
1113	4	-0.5563585403934752
1113	5	-0.6261626589938771
1113	6	-0.5645823307225513
1113	7	-0.6394749300498646
1113	8	-0.5924977770305446
1113	9	-0.6588865238191133
1113	10	-0.5866700812437884
1113	11	-0.5075770918858851
1113	12	-0.4998622850983949
1113	13	-0.5359910637112648
1113	14	-0.6790558733496397
1113	15	-0.6749967454188166
1113	16	-0.7485429800109458
1113	17	-0.7396349348380369
1113	18	-0.7578462366388052
1113	19	-0.9628243229406124
1113	20	-0.973419589042777
1113	21	-0.9397131468189812
1113	22	-0.9346789537211748
1113	23	-0.9914581455699789
1113	24	-1.139092859531118
1113	25	-1.0926951471822548
1114	1	-0.8543336657387215
1114	2	-0.8876048544610669
1114	3	-0.8929888499952519
1114	4	-0.7941691582663353
1114	5	-0.8685537036421991
1114	6	-0.8794520759582412
1114	7	-0.8268538973201061
1114	8	-0.8441879884847907
1114	9	-0.9471974310242501
1114	10	-0.8547558340808337
1114	11	-0.8757065553022656
1114	12	-1.066955538477508
1114	13	-0.9255622580942173
1114	14	-1.0234876427235093
1114	15	-1.0041831900292948
1114	16	-0.9724191761823704
1114	17	-1.4242260924571397
1114	18	-1.1629907577628202
1114	19	-1.0981769285192726
1114	20	-0.9659057887911473
1114	21	-1.104084190942494
1114	22	-1.128861786857243
1114	23	-1.3196061013926013
1114	24	-1.2911215690263305
1114	25	-1.1502768956330485
1115	1	-1.38030043440002
1115	2	-1.3287154851294112
1115	3	-1.5017378963775627
1115	4	-1.6522318753262475
1115	5	-1.499834153997618
1115	6	-1.4232848892199286
1115	7	-1.329671235122472
1115	8	-1.4864013730082277
1115	9	-2.0763965849553543
1115	10	-1.8050484604472246
1115	11	-1.8309531739185374
1115	12	-1.6320908626799806
1115	13	-1.6111945100641094
1115	14	-1.7834748084753471
1115	15	-1.9585082765778523
1115	16	-2.0766396057716463
1115	17	-1.956467458946534
1115	18	-1.7729578183619523
1115	19	-1.835708979713047
1115	20	-1.9645882127564889
1115	21	-1.8431566273289661
1115	22	-2.0136080350429637
1115	23	-1.9960544297730891
1115	24	-2.129435930990681
1115	25	-1.957064037148534
1116	1	-1.0308939859772295
1116	2	-1.154559385632556
1116	3	-1.0229797875004714
1116	4	-1.1992704927452564
1116	5	-0.7396852555450977
1116	6	-0.8118161119714102
1116	7	-0.8013871484787245
1116	8	-0.925966150149371
1116	9	-1.2429648512796092
1116	10	-1.8039949336604977
1116	11	-1.3725248909910377
1116	12	-1.1070816695815964
1116	13	-1.157176702830455
1116	14	-1.2221574142180884
1116	15	-1.9417273450237644
1116	16	-1.6450665328029288
1116	17	-1.5557908225025727
1116	18	-1.6201950616129395
1116	19	-1.4590695311250812
1116	20	-1.5125296679335918
1116	21	-1.4534561919322329
1116	22	-1.4342118465907703
1116	23	-1.5319184948475666
1116	24	-1.3571731903324533
1116	25	-1.6148744950586362
1201	1	1.0069853552438772
1201	2	1.1645798128608114
1201	3	0.9850344087925578
1201	4	1.0502879722729688
1201	5	1.016722489896054
1201	6	1.0083445503118398
1201	7	0.8982994623434735
1201	8	1.0546779355277351
1201	9	1.1747827811034952
1201	10	1.2891150770892992
1201	11	1.1801465044011366
1201	12	1.1419912030635047
1201	13	1.1578248439835832
1201	14	1.3631230265055478
1201	15	1.3306978671750471
1201	16	1.3213554627510717
1201	17	1.2635517177970428
1201	18	1.4472595865885367
1201	19	1.410499446968032
1201	20	1.364345249743323
1201	21	1.3971264278669437
1201	22	1.5150051053580427
1201	23	1.5333608006873902
1201	24	1.4812532313960318
1201	25	1.5083240331108811
1202	1	0.8155212517440269
1202	2	0.8388333212958372
1202	3	0.774241089466976
1202	4	0.9047167960378744
1202	5	0.7992609797276771
1202	6	0.7674060245122121
1202	7	0.8254233563392575
1202	8	0.8518147961808893
1202	9	0.8991186285302746
1202	10	0.9251289037263817
1202	11	0.9073530961121321
1202	12	1.018922510390503
1202	13	1.0543328863244312
1202	14	1.114410724156194
1202	15	1.1640014663192702
1202	16	1.019280654082209
1202	17	1.1230918725838208
1202	18	1.277655851655524
1202	19	1.1715023325395464
1202	20	1.1446056064483656
1202	21	1.188544519104191
1202	22	1.22792135012606
1202	23	1.241725801330702
1202	24	1.2819378670788737
1202	25	1.2623906418397226
1203	1	0.8533104869706811
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2211	8	-0.5518485010226755
2211	9	-0.556717960095618
2211	10	-0.673934671226057
2211	11	-0.661336205343602
2211	12	-0.6558092171768735
2211	13	-0.6445206488354671
2211	14	-0.7866455374603518
2211	15	-0.7157152925368777
2211	16	-0.6333450510790399
2211	17	-0.6832368392193331
2211	18	-0.7766446569085943
2211	19	-0.8046387637355821
2211	20	-0.7587963922631786
2211	21	-0.7415738575173094
2211	22	-0.7833396044378418
2211	23	-0.8458392234647931
2211	24	-0.7265307726636934
2211	25	-0.7367607754897563
2212	1	-0.6671606772878
2212	2	-0.7159322098768577
2212	3	-0.6144455633698875
2212	4	-0.6134162254321183
2212	5	-0.6350956148007114
2212	6	-0.5780633670318025
2212	7	-0.5924651613399732
2212	8	-0.5490867008712499
2212	9	-0.644335363580808
2212	10	-0.760593879266203
2212	11	-0.8067525935732789
2212	12	-0.7214468791590036
2212	13	-0.6847961828593085
2212	14	-0.8829501000249422
2212	15	-0.9071564624016375
2212	16	-0.9597758449896006
2212	17	-0.8214880448511792
2212	18	-0.8831513855224742
2212	19	-0.8795634873888218
2212	20	-0.8455427566698646
2212	21	-0.8157805715851509
2212	22	-0.7996856573298459
2212	23	-0.8871201657547658
2212	24	-0.9264339039453873
2212	25	-1.0349484851231168
2213	1	-0.921514861701624
2213	2	-0.7719337994223032
2213	3	-0.764405451141247
2213	4	-0.8202971030306685
2213	5	-0.8088208464335054
2213	6	-0.8026807484558987
2213	7	-0.9136254482677231
2213	8	-0.9674559035588288
2213	9	-0.9387993101990091
2213	10	-1.122751627723538
2213	11	-1.112053769707785
2213	12	-1.0568159410421032
2213	13	-1.085500335822445
2213	14	-1.214034092108264
2213	15	-1.216201543265555
2213	16	-1.2272941236378188
2213	17	-1.1522617012547371
2213	18	-1.0663387360718701
2213	19	-1.1941414370304386
2213	20	-1.141809450360931
2213	21	-1.1670593787412642
2213	22	-1.134302818950971
2213	23	-1.0316154469912178
2213	24	-1.005167618304391
2213	25	-0.9110980709831225
2214	1	-1.3736379715844507
2214	2	-1.2919020990416499
2214	3	-1.2901998583550132
2214	4	-1.3944209499409794
2214	5	-1.4508992922243138
2214	6	-1.5301028499228408
2214	7	-1.5008916091927418
2214	8	-1.5145345592625254
2214	9	-1.5563344861576098
2214	10	-2.1640736520527923
2214	11	-1.9477637834574324
2214	12	-1.8139620614772554
2214	13	-1.8014027428660242
2214	14	-1.8650891765024369
2214	15	-1.9132661411664529
2214	16	-1.87810897438834
2214	17	-1.904452746582777
2214	18	-1.8928435394018663
2214	19	-1.934519970051717
2214	20	-1.9224441873683489
2214	21	-1.8839207413826813
2214	22	-1.8619925770252976
2214	23	-2.0076677078096097
2214	24	-1.9868831452005935
2214	25	-1.9149329745638397
2215	1	-1.6802054270489641
2215	2	-1.4387948911550303
2215	3	-1.581427454729667
2215	4	-2.2402894428682956
2215	5	-1.7724851597660631
2215	6	-1.602291271375968
2215	7	-1.6874319391154238
2215	8	-1.6891078882476194
2215	9	-1.7613646011141952
2215	10	-1.8441752751691212
2215	11	-1.9007306077477892
2215	12	-1.9256477007364268
2215	13	-1.8427751390178315
2215	14	-1.9772775809702523
2215	15	-2.053716404778484
2215	16	-2.0199129672952694
2215	17	-2.054571049274614
2215	18	-2.4488585476227556
2215	19	-2.88980444004207
2215	20	-2.4482669558381787
2215	21	-2.19561521300362
2215	22	-3.131936304689617
2215	23	-2.5166784153497304
2215	24	-2.5898901397517022
2215	25	-2.40797451723774
2216	1	-2.6133153042609543
2216	2	-5.433449248925584
2216	3	-2.9219911359623616
2216	4	-5.507263079560243
2216	5	-3.252370346957587
2216	6	-2.0819620309763067
2216	7	-1.8363274489264256
2216	8	-1.6571845103735363
2216	9	-1.8759705057729121
2216	10	-1.9338699603743734
2216	11	-1.7028327505189438
2216	12	-1.885805549538638
2216	13	-1.8175291115710976
2216	14	-2.5301734820175596
2216	15	-2.234192960695175
2216	16	-2.401921363828315
2216	17	-2.0875289462289857
2216	18	-2.020891170711721
2216	19	-2.052987598823819
2216	20	-2.4869692551038582
2216	21	-2.650295753511628
2216	22	-2.408754801138393
2216	23	-2.4520868058637504
2216	24	-2.4607182064424435
2216	25	-2.6437357728053286
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87898.0
3	3.0
4	555.0
5	3737.0
6	6659.0
7	6757.0
8	5656.0
9	4211.0
10	4235.0
11	4828.0
12	4574.0
13	3870.0
14	3380.0
15	3307.0
16	3231.0
17	3525.0
18	4337.0
19	6281.0
20	8463.0
21	10440.0
22	11821.0
23	13418.0
24	14546.0
25	15974.0
26	17135.0
27	18770.0
28	20731.0
29	24454.0
30	30408.0
31	38950.0
32	49713.0
33	65296.0
34	89397.0
35	125596.0
36	187804.0
37	332913.0
38	917981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.93618570438427	18.260013208590717	12.64248825578926	24.16131283123575
2	16.427272744295085	21.70405031672649	38.17719831403764	23.691478624940785
3	18.398840369089246	26.396304612866434	29.310879218222986	25.893975799821334
4	12.19368067355953	16.965648220509177	38.49517638514749	32.345494720783805
5	14.969448861965425	36.31155417441684	32.92255071095552	15.79644625266222
6	30.7248437303902	38.487046059495775	16.160347511393585	14.627762698720433
7	27.540028430314116	32.35692924505104	21.766613818444107	18.336428506190735
8	26.323356546417582	32.76547916208103	20.05011028946888	20.86105400203251
9	27.356981505276444	15.153582626872605	19.512083194413744	37.9773526734372
10	16.301407642181786	26.716311615073096	30.613209071128466	26.369071671616645
11	34.6524031115205	23.961620646814513	22.825824814708497	18.56015142695649
12	24.57452532897864	25.57910496829282	28.484054622232442	21.362315080496096
13	28.85839565370712	21.89916626743226	25.160946492286694	24.08149158657392
14	23.375291314154683	22.135988444850767	25.790248188419373	28.69847205257518
15	24.093822123310254	28.643257500824266	23.507890477475048	23.755029898390433
16	23.662974261183287	28.12579904035546	24.17225272949844	24.038973968962814
17	23.58664823286537	26.54617255087092	25.10991903364918	24.757260182614527
18	25.228272435705296	24.761423925424136	26.94143973682633	23.068863902044242
19	26.243923640790044	25.50766491235383	26.04271705698839	22.20569438986773
20	25.34840035798936	25.417244465425505	25.755329915480978	23.479025261104155
21	25.55147962746236	25.749812321541555	25.85710398674473	22.841604064251364
22	24.263198173767485	25.699613977010976	26.213124781626767	23.82406306759477
23	23.09631108099362	25.407230039604915	27.103446241139	24.39301263826246
24	23.33273543272939	26.60303318254686	26.637970559873814	23.426260824849933
25	23.382068906086374	26.105846957375338	26.768549585143468	23.743534551394813
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11883.0
1	11883.0
2	10006.0
3	8129.0
4	8129.0
5	8129.0
6	8896.5
7	9664.0
8	9664.0
9	9664.0
10	10718.5
11	11773.0
12	11773.0
13	11773.0
14	12788.5
15	13804.0
16	13804.0
17	13804.0
18	22415.5
19	31027.0
20	31027.0
21	31027.0
22	43403.5
23	55780.0
24	55780.0
25	55780.0
26	74650.0
27	93520.0
28	93520.0
29	93520.0
30	108784.0
31	124048.0
32	124048.0
33	124048.0
34	140615.0
35	157182.0
36	157182.0
37	157182.0
38	169965.0
39	182748.0
40	182748.0
41	182748.0
42	200295.5
43	217843.0
44	217843.0
45	217843.0
46	230134.0
47	242425.0
48	242425.0
49	242425.0
50	247229.0
51	252033.0
52	252033.0
53	252033.0
54	234057.5
55	216082.0
56	216082.0
57	216082.0
58	197367.5
59	178653.0
60	178653.0
61	178653.0
62	156438.5
63	134224.0
64	134224.0
65	134224.0
66	110873.5
67	87523.0
68	87523.0
69	87523.0
70	68103.5
71	48684.0
72	48684.0
73	48684.0
74	40001.0
75	31318.0
76	31318.0
77	31318.0
78	26816.0
79	22314.0
80	22314.0
81	22314.0
82	16900.0
83	11486.0
84	11486.0
85	11486.0
86	8685.5
87	5885.0
88	5885.0
89	5885.0
90	4229.5
91	2574.0
92	2574.0
93	2574.0
94	1386.0
95	198.0
96	198.0
97	198.0
98	126.0
99	54.0
100	54.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38631166969027186
2	0.6800089638813235
3	0.6974903921884051
4	0.7073934353517254
5	0.7168315469111339
6	0.7179473827605221
7	0.7350103726240832
8	0.7236195483282454
9	0.7125541761551458
10	0.7046038457282549
11	0.7176219306377839
12	0.722178260356119
13	0.7214343697898602
14	0.7300356044622276
15	0.7263161516309335
16	0.7197141228553867
17	0.7274319874803217
18	0.7292917138959688
19	0.7315698787551363
20	0.7250608363003719
21	0.7231081235639425
22	0.7321742898402216
23	0.7212483971482955
24	0.7255257679042836
25	0.7279899054050158
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2150854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.37372800025991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.0144459851056	45.216302081323825
2	7.304198505757007	7.504878145893308
3	1.6434357855492858	2.5328826909810744
4	0.7344725666001797	1.5093037544068166
5	0.42137018384079616	1.0823678606028289
6	0.27130737983289416	0.8362842921598983
7	0.20607021694475447	0.7410616691992057
8	0.15132748441037408	0.6219405618449705
9	0.12234197545306796	0.5656647032946349
>10	0.8517503177290655	8.850114117789175
>50	0.13253563288671302	4.789186985292888
>100	0.1256507542702194	13.703315766620017
>500	0.014398409671129292	5.0161552861279
>1k	0.006236253870298037	5.1229641943685005
>5k	3.668384629587081E-4	1.280434803376614
>10k+	9.170961573967703E-5	0.6271430867184056
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13311	0.6188704579669284	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8571	0.3984928777127597	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6860	0.31894308028345947	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6326	0.29411573263457214	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5420	0.2519929293201677	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4376	0.20345406987178116	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4245	0.19736346586053727	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	3263	0.15170718235640357	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2409	0.11200202338234022	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2377	0.11051424224982262	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2369	0.11014229696669324	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2363	0.1098633380043462	No Hit
GTACAAAGGGCAGGGACTTAATCAA	2329	0.10828257055104623	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2270	0.1055394740879669	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2259	0.105028049323664	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2216	0.10302884342684349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	4.649316039117485E-5	0.0	0.0	0.0
5	0.0	4.649316039117485E-5	0.0	0.0	0.0
6	4.649316039117485E-5	4.649316039117485E-5	0.0	0.0	0.0
7	4.649316039117485E-5	4.649316039117485E-5	4.649316039117485E-5	0.0	0.0
8	1.859726415646994E-4	4.649316039117485E-5	4.649316039117485E-5	0.0	0.0
9	1.859726415646994E-4	4.649316039117485E-5	4.649316039117485E-5	0.0	0.0
10	1.859726415646994E-4	4.649316039117485E-5	4.649316039117485E-5	0.0	0.0
11	1.859726415646994E-4	4.649316039117485E-5	4.649316039117485E-5	4.649316039117485E-5	0.0
12	1.859726415646994E-4	4.649316039117485E-5	4.649316039117485E-5	4.649316039117485E-5	0.0
13	2.3246580195587427E-4	4.649316039117485E-5	4.649316039117485E-5	1.3947948117352455E-4	4.649316039117485E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	60	2.569924E-5	14.249543	5
TTACCGG	105	1.373337E-9	13.570675	11
GTAACAC	100	1.4440047E-7	12.349604	3
CCGGTAG	70	1.0920731E-4	12.213322	9
GCTCGTA	165	0.0	12.089956	9
TTAGAAC	160	3.6379788E-12	11.8746195	3
CTCGTAG	185	0.0	11.809878	10
CGGTAGC	65	8.030893E-4	11.691384	10
GTATCAA	8090	0.0	11.68191	1
CGGTCCA	440	0.0	11.44228	10
ATCGATG	100	1.9323343E-6	11.399101	8
CGTCAAT	285	0.0	11.332705	19
CGACCAT	345	0.0	11.288965	10
GTAAGGC	85	5.3265278E-5	11.176113	3
GGTCCAA	480	0.0	11.082719	11
GGTATCA	3155	0.0	11.059684	1
GTATTAT	190	0.0	11.008929	1
CCGTCGT	360	0.0	10.818591	9
CGCCAGT	220	0.0	10.794855	18
CCGACCA	370	0.0	10.782932	9
>>END_MODULE