FastQCFastQC Report
Thu 2 Feb 2017
SRR4050262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2150854
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT218551.0161080203491264No Hit
TATCAACGCAGAGTACTTTTTTTTT165720.7704846540025497No Hit
GTACTTTTTTTTTTTTTTTTTTTTT118300.5500140874275985No Hit
ACGCAGAGTACTTTTTTTTTTTTTT97240.45209949164378427No Hit
GGTATCAACGCAGAGTACTTTTTTT88140.40979071568781517No Hit
GAGTACTTTTTTTTTTTTTTTTTTT82160.38198780577389263No Hit
GCAGAGTACTTTTTTTTTTTTTTTT47350.2201451144522129No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA27690.12873956112316318No Hit
ATCAACGCAGAGTACTTTTTTTTTT26670.12399725876326333No Hit
GAATAGGACCGCGGTTCTATTTTGT24940.1159539420155901No Hit
GATTAAGAGGGACGGCCGGGGGCAT24530.11404772243955191No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG23800.11065372173099616No Hit
CTATTGGAGCTGGAATTACCGCGGC23580.10963087220239032No Hit
GTACAAAGGGCAGGGACTTAATCAA22810.10605089885226986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26450.014.3902251
ACGACTA751.46144575E-512.6815585
GTATCAA76150.012.6398411
ACTGTCG1001.9459894E-611.3924398
TCGCAGT1105.0137896E-711.21982716
GTATTAG4850.011.2112361
CGAGCCG4850.011.15754415
CGGTCCA3700.011.03321810
TGTCGAG951.3707975E-510.99270410
AGGCCCG5200.010.95426810
ATACCGT3750.010.890166
CGACCAT3150.010.84994310
GCGAAAC1252.2609493E-710.63319119
GCCGCCT5300.010.568459518
AGAACCG2800.010.5302215
ATCGCCA4150.010.52313616
TATTGGG1002.3668079E-510.4630162
TAATACC1553.594323E-910.4316034
TAGACAG2750.010.3758195
CTGTCGA1106.0956027E-610.3567639
Produced by FastQC (version 0.11.5)